Fig 2: HOXA5 arrests cell cycle transition from G0/G1 to S phase through cyclinD1 and p21.a Volcano plots of the data from RNA-seq. The expression of cyclinD1 and p21 in HOXA5-modified cervical cancer cells and xenograft was determined by real-time PCR and western blot (b–e). The expression of cyclinD1 and p21 in xenograft was determined by western blot (f) and IHC (g, h). *p < 0.05, **p < 0.01, ***p < 0.001.

Fig 3: p53 mutational status affects the basal expression of its target genes.(A) Gene set enrichment analysis (GSEA) of direct p53 target genes identified by GRO-seq (left) or genes upregulated upon Nutlin treatment in the microarray experiment only (right) relative to a ranked list of 12,624 genes analyzed via Oncomine for their relative expression in WT p53 vs mutant p53 cell lines. (B) Scatter plot comparing relative transcription as measured by GRO-seq in HCT116 p53 +/+ over p53 −/− cells vs relative mRNA expression in p53 WT vs p53 mutant cell lines. Red dots are genes whose transcription was lesser than twofold in p53 +/+ cells, green dots are genes whose transcription was greater than twofold in p53 +/+ cells, all other direct p53 targets in HCT116 cells are highlighted in black. (C) Oncomine gene expression analysis of 247 breast carcinomas of varied p53 status shows that CDKN1A, DDB2 and GDF15 are more highly expressed in wild type p53 tumors, whereas GJB5 is more highly expressed in mutant p53 tumors. (D) Model depicting the differential effects of MDM2-p53 complexes on the basal expression of p53 target genes.DOI: http://dx.doi.org/10.7554/eLife.02200.010

Fig 4: Overexpression of miR-494 regulates the p53 signaling pathway in spinal cord injury. (A) p53, (B) p21, (C) Bax and (D) cyclin E protein expression levels were semi-quantified following (E) western blotting. (F and G) Caspase-3/9 activity levels were detected. **P<0.01 compared with the negative mimics group. Bax, B-cell lymphoma 2-associated X protein; miR-494, microRNA-494.

Fig 5: Knockdown of miR-494 regulates the p53 signaling pathway in spinal cord injury. (A) p53, (B) p21, (C) Bax and (D) cyclin E protein expression levels were semi-quantified following (E) western blotting. (F and G) Caspase-3/9 activity levels were detected. **P<0.01 compared with the negative mimics group. Bax, B-cell lymphoma 2-associated X protein; miR-494, microRNA-494.