Santa Cruz Biotechnology, Inc.
p21
sc-187
187
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The resistance to endoreduplication observed in some cell lines was associated with a prolonged 4N arrest after Nutlin-3a removal. Knockdown of either p53 or p21 immediately after Nutlin-3a removal could drive endoreduplication in otherwise resistant 4N cells. Finally, 4N-arrested cells retained persistent p21 expression; expressed senescence-associated β-galactosidase; displayed an enlarged, flattened phenotype; and underwent a proliferation block that lasted at least 2 weeks after Nutlin-3a removal.
Western Blot
Cell lysate
1:1000 dilution in 5% skim milk prepared in 1x TBST
5% skim milk
1:4000 5% milk
None
ECL
The resistance to endoreduplication observed in some cell lines was associated with a prolonged 4N arrest after Nutlin-3a removal. Knockdown of either p53 or p21 immediately after Nutlin-3a removal could drive endoreduplication in otherwise resistant 4N cells.
N/A
No background and strong signal
This antibody is highly specific and works great for western blot. Highly recommended.