ASC/TMS1 Antibody

Product Specs
ItemASC/TMS1 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $469.00Supplier Page View Company Product Page
Catalog NumberNBP1-78977
Concentration1.0 mg/ml
ImmunogenThis ASC/TMS1 Antibody was developed against a synthetic peptide made to an N-terminal portion of the human ASC/TMS1 protein (between residues 1-50) [Uniprot: Q9ULZ3]
Quantity0.1 ml (also 0.025 ml)
FormatPBS
HostRabbit
ReactivityHuman,Mouse,Rat
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaApoptosis, Cancer, Cell Biology, Inflammation, Innate Immunity
IsotypeIgG
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene NamePYD and CARD domain containing
NCBI Gene AliasesASC, TMS, TMS1, CARD5, TMS-1
ApplicationsWB, FCM, ICC, IF, IHC, IHC-p, IM, IP
TargetASC/TMS1
Conjugate/TagUnconjugated

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this ASC/TMS1 antibody be used for immunofluorescence applications?

Yes, the ASC/TMS1 antibody has been successfully utilized in immunofluorescence applications, as demonstrated in studies where it was employed to visualize ASC in human keratinocytes and microglial cells under various experimental conditions (, ).

What species does this antibody react with?

The ASC/TMS1 antibody shows reactivity with multiple species, including human, mouse, and rat, making it versatile for studies involving these organisms.

Has this antibody been cited in any relevant research studies?

Yes, the ASC/TMS1 antibody has been cited in at least 37 scientific articles, indicating its utility and relevance in various research contexts, particularly in studies related to inflammasome activation and inflammation (, ).

What specific applications has this antibody been shown to work in?

The ASC/TMS1 antibody has been shown to work in several applications, including Western blotting, ELISA, immunocytochemistry, and immunohistochemistry, as evidenced by its use in various studies examining inflammasome pathways and inflammatory responses (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) LXR pathway drives hormonal response intensity in polycystic ovary syndromeEMBO Molecular MedicineMay 21, 2025Sarah Dallel, Manon Despalles, Margaux Tore, Yoan Renaud, Ayhan Kocer, Christelle Damon-Soubeyrand, Pierre Pouchin, Caroline Vachias, Katia Boutourlinsky, Céline Gonthier-Gueret, et al.for western blot,,Clone,Reference,MW (kDa),Dil.,Host ACTIN,Sigma-Aldrich,,A2066,42,1/5000,Rabbit ASC/PYCARD,Novus Biologicals,,NBP1-78977,22,1/1000,Rabbit IL1B,R&D Systems,IL-IF2,AF-401-NA,17,1/800,Mouse NLRP3,Novus Biologicals,,NBP1-77080,110,1/1000,Rabbit TXNIP,Novus

(2) DAG-MAG-ΒHB: A Novel Ketone Diester Modulates NLRP3 Inflammasome Activation in Microglial Cells in Response to Beta-Amyloid and Low Glucose AD-like ConditionsNutrientsDecember 31, 2024Valentina Gentili, Giovanna Schiuma, Latha Nagamani Dilliraj, Silvia Beltrami, Sabrina Rizzo, Djidjell Lara, Pier Paolo Giovannini, Matteo Marti, Daria Bortolotti, Claudio Trapella, et al.overnight at 4 °C. After washing with PBS, the cells were incubated with anti-ASC rabbit antibody (1:500, NBP1-78977, Novus) and Alexa Fluor 594-conjugated anti-rabbit IgG secondary antibody for 1 h. Cell nuclei were

(3) Dihydromyricetin treats pulmonary hypertension by modulating CKLF1/CCR5 axis-induced pulmonary vascular cell pyroptosisBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieNovember 1, 2024Yan Q, Li P, Liu S, Sun Y, Chen C, Long J, Lin Y, Liang J, Wang H, Zhang L, et al.

(4) Combined exposure to UV and PM affect skin oxinflammatory responses and it is prevented by antioxidant mix topical application: Evidences from clinical studyJournal of cosmetic dermatologyAugust 1, 2024Ferrara F, Yan X, Pecorelli A, Guiotto A, Colella S, Pasqui A, Lynch S, Ivarsson J, Anderias S, Choudhary H, et al.

(5) Long Non-coding RNA NEAT1 , NOD-Like Receptor Family Protein 3 Inflammasome, and Acute Kidney InjuryJournal of the American Society of NephrologyAugust 1, 2024Rui Xue, Wai Han Yiu, Kam Wa Chan, Sarah W.Y. Lok, Yixin Zou, Jingyuan Ma, Hongyu Li, Loretta Y.Y. Chan, Xiao Ru Huang, Kar Neng Lai, et al.permeabilized with 0.25% Triton X-100, and blocked with 5% BSA. Cells were incubated with anti-ASC antibody (NBP1-78977, Novus Biologicals, Littleton, CO) overnight at 4°C, followed by Alexa Fluor 594–conjugated secondary

(6) NINJ1 mediates plasma membrane rupture by cutting and releasing membrane disksCellApril 25, 2024David L, Borges JP, Hollingsworth LR, Volchuk A, Jansen I, Garlick E, Steinberg BE, Wu H.cells were incubated overnight at 4 °C with primary antibodies (human ASC 1:1000, Novus Biologicals, NBP1–78977 and human ASC-Phycoerythrin (PE) 1:1000, BioLegend 653903). NINJ1 was detected by the GFP signal, and

(7) MCC950 Ameliorates Diabetic Muscle Atrophy in Mice by Inhibition of Pyroptosis and Its Synergistic Effect with Aerobic ExerciseMolecules (Basel, Switzerland)February 4, 2024Yan X, Fu P, Zhang Y, Ling D, Reynolds L, Hua W, Wang Z, Ma F, Li B, Yu J, et al.blocking buffer (LI-COR), and then incubated with anti-NLRP3 (CST, 15101S), anti-ASC (Novus Biologicals, NBP1-78977, Centennial, CO, USA), anti-Caspase-1 (CST, 2225S), anti-GSDMDC1 (Santa Cruz, sc-393656, Santa Cruz,

(8) L-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitusJournal of reproductive immunologyFebruary 1, 2024McElwain CJ, Musumeci A, Manna S, McCarthy FP, McCarthy CM.

(9) Ginsenoside Rg1 treats ischemic stroke by regulating CKLF1/CCR5 axis-induced neuronal cell pyroptosisPhytomedicine : international journal of phytotherapy and phytopharmacologyJanuary 1, 2024Long J, Sun Y, Liu S, Chen C, Yan Q, Lin Y, Zhang Z, Chu S, Yang Y, Yang S, et al.

(10) Vitamin C compounds mixture prevents skin barrier alterations and inflammatory responses upon real life multi pollutant exposureExperimental dermatologyJanuary 1, 2024Ferrara F, Pecorelli A, Pambianchi E, White S, Choudhary H, Casoni A, Valacchi G.

(11) Toxoplasma gondii Induces Pyroptosis in Human Placental Trophoblast and Amniotic Cells by Inducing ROS Production and Activation of Cathepsin B and NLRP1/NLRP3/NLRC4/AIM2 InflammasomeThe American journal of pathologyDecember 1, 2023Quan JH, Gao FF, Ma TZ, Ye W, Gao X, Deng MZ, Yin LL, Choi IW, Yuk JM, Cha GH, et al.

(12) Comparison of Pollutant Effects on Cutaneous Inflammasomes ActivationInternational journal of molecular sciencesNovember 23, 2023Ivarsson J, Ferrara F, Vallese A, Guiotto A, Colella S, Pecorelli A, Valacchi G.Littleton, CO, USA) 1:200, NLRC4 (NB100-561425, Novus Biological, Littleton, CO, USA) 1:200, ASC (cat. NBP1-78977, Novus Biological, Littleton, CO, USA) 1:150 or ASC (sc-271054, Santa Cruz Biotechnology Inc., Dallas,

(13) Site-specific Atg13 methylation-mediated autophagy regulates epithelial inflammation in PM2.5-induced pulmonary fibrosisJournal of hazardous materialsSeptember 5, 2023Ning J, Pei Z, Wang M, Hu H, Chen M, Liu Q, Wu M, Yang P, Geng Z, Zheng J, et al.

(14) Asiatic acid alleviates cisplatin-induced renal fibrosis in tumor-bearing mice by improving the TFEB-mediated autophagy-lysosome pathwayBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieSeptember 1, 2023Chen XC, Huang LF, Tang JX, Wu D, An N, Ye ZN, Lan HY, Liu HF, Yang C.

(15) TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4NeuronJanuary 18, 2023Gratuze M, Schlachetzki JCM, D'Oliveira Albanus R, Jain N, Novotny B, Brase L, Rodriguez L, Mansel C, Kipnis M, O'Brien S, et al.

(16) Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB PathwayCellsNovember 13, 2022Li T, Li L, Peng R, Hao H, Zhang H, Gao Y, Wang C, Li F, Liu X, Chen F, et al.Abclonal, Wuhan, China); phospho-NF-κB p65 (1:1000, #3033; Cell Signaling, Danvers, MA, USA); ASC (1:1000, NBP1-78977; NOVUS, Colorado, USA); caspase-1 (1:1000, A0964; Abclonal, Wuhan, China); GSDMD (1:1000 A20728, Abclonal,

(17) Ubiquitination as a key regulatory mechanism for O(3)-induced cutaneous redox inflammasome activationRedox biologyOctober 1, 2022Ferrara F, Cordone V, Pecorelli A, Benedusi M, Pambianchi E, Guiotto A, Vallese A, Cervellati F, Valacchi G.for 30 min as previously described [44]. Coverslips were incubated with primary antibody ASC (Cat. NBP1-78977 Novus Biological, Littleton, CO, USA) 1:100, NLRP1 (sc-166368 Santa Cruz Biotechnology Inc., Dallas,

(18) RvD1 disrupts nociceptor neuron and macrophage activation and neuroimmune communication, reducing pain and inflammation in gouty arthritis in miceBritish journal of pharmacologySeptember 1, 2022Zaninelli TH, Fattori V, Saraiva-Santos T, Badaro-Garcia S, Staurengo-Ferrari L, Andrade KC, Artero NA, Ferraz CR, Bertozzi MM, Rasquel-Oliveira F, et al.

(19) Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathwayAging cellMay 1, 2022Jung ES, Suh K, Han J, Kim H, Kang HS, Choi WS, Mook-Jung I.MA1‐19332, Thermo Fisher Scientific), pASC (1:1000, AP5631, ECM Biosciences, Kentucky, USA), ASC (1:1000, NBP1‐78977, Novus Biologicals, USA), Mfn2 (1:500, sc‐100560, Santa Cruz, Texas, USA), Drp1 (1:500, sc‐101270, Santa Cruz

(20) Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathwayLupus science & medicineApril 1, 2022Chen XC, Wu D, Wu HL, Li HY, Yang C, Su HY, Liu ZJ, Huang XR, Lu X, Huang LF, et al.(ab205421; Abcam, USA), phospho-RIP (Ser166) (44 590S; Cell Signaling Technology, USA), ASC/TMS1 Antibody (NBP1-78977; Novus, USA), anti-caspase-1 antibody (ab1872; Abcam, USA), phospho-AMPKα (Thr172) (2535; Cell Signaling Technology

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(1)

6/6/2016ASC Antibody for Immunocytochemistry KAVITHA SUBRAMANIANNBP1-78977 was used to detect ASC protein in primary bone marrow derived macrophages. This product was used because of reasonable pricing and citations in literature for its use in microscopy.