Novus Biologicals
ASC/TMS1 Antibody
NBP1-78977
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Browse Similar Products: ASC Western Blot Antibodies
NBP1-78977 was used to detect ASC protein in primary bone marrow derived macrophages. This product was used because of reasonable pricing and citations in literature for its use in microscopy.
Western Blot
Primary bone marrow derived macrophages (murine)
1:1000 dilution, overnight at 4 deg on a shaker
5% Skim non-fat milk
Goat antirabbit HRP secondary Ab, 1:1000 dilution, 2h at ambient temperature on a shaker
NA
Chemiluminiscence using SuperSignal West Femto Maximum Sensitivity Substrate
ASC Band is seen at ~21 kDa (bottom band is ~21 kD size).
None
Works great for Western Blotting
ASC/TMS1 Antibody [NBP1-78977] works well for western blotting on murine primary macrophage lysates.