SOX9 Antibody AF3075 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSOX9 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3075
ApplicationsIF/ICC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunofluorescence,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
HostGoat
TargetSOX9
IsotypeIgG
ReactivityAvian,Avian - Chicken,Chicken,Human,Monkey,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Rat,Transgenic Mouse,Turtle,Xenograft
NCBI Full Gene NameSRY-box transcription factor 9
ConcentrationLYOPH
PurityAntigen Affinity-purified
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
ImmunogenE. coli-derived recombinant human SOX9, Met1-Lys151, Accession # P48436
ClonalityPolyclonal
Conjugate/TagUnconjugated
NCBI Gene AliasesSRXY10, CMPD1, CMD1, SRXX2, SRA1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human SOX10 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study SOX9 in mouse models, and if so, how has it been applied in research?

Yes, the SOX9 antibody has been shown to work effectively in mouse models. For instance, in a study by Caramello et al. (2021), the antibody was utilized to investigate the role of SOX9 in the development of the dentate gyrus, highlighting its importance in regulating astroglial potential acquisition during brain morphogenesis.

What applications is the SOX9 antibody suitable for, and are there any specific protocols that have utilized it?

The SOX9 antibody is suitable for various applications, including Western blotting, immunocytochemistry, immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation. It has been specifically used in immunostaining protocols to analyze astrocyte nuclei in mouse brain tissue, as described in the STAR Protocols article by Labarta-Bajo et al. (2023).

Is there any evidence of cross-reactivity with other proteins when using this antibody, and how might that affect experimental results?

The SOX9 antibody exhibits approximately 25% cross-reactivity with recombinant human SOX10 in direct ELISAs. This cross-reactivity could potentially affect experimental results, particularly in assays where specificity is crucial, such as in distinguishing between SOX9 and SOX10 expression levels.

How has the SOX9 antibody contributed to our understanding of human embryogenesis?

The SOX9 antibody has been instrumental in studies like that of Blain et al. (2023), where it was used in advanced whole-mount immunostaining to create a 3D cellular atlas of human head embryogenesis. This research provided valuable insights into the development of various head tissues and has implications for understanding craniofacial defects and neurological disorders.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Brain injury reactivates a developmental program driving genesis and integration of transient LGE-class interneuronsStem Cell ReportsAugust 11, 2026G. Nato, M. Fogli, N. Marichal, V. Cerrato, G. Turrini, V. Proserpio, I. Ghia, G. Zanotto, I. Molineris, M. Bergami, et al.(1:1000),FUJIFILM Wako,Cat# 019–19741;RRID:AB_839504 Goat polyclonal anti-SOX9 (1:1200),R&D Systems,Cat# AF3075; RRID:AB_2194160 Goat polyclonal SOX10 (clone N-20) (1:750),Santa Cruz Biotechnology,Cat#sc-17342;RRID:

(2) Spatio-Temporal Dynamics of Macroglial Cell Organization and Proximity to Blood Vessels During Postnatal DevelopmentInt J Dev NeurosciJuly 28, 2026Naomie Guille, Héloïse Monnet, Tristan Hourcade, Philippe Mailly, Martine Cohen-Salmon, Anne-Cécile BoulayAL). 2.4 Primary and Secondary Antibodies Primary antibodies used were polyclonal goat IgG anti‐Sox9 (AF3075 Biotechne/R&D, 1:500), monoclonal mouse/IgG2a anti‐Sox10 (66786‐1‐Ig Proteintech, 1:500), AlexaF647‐coupled

(3) Clec3b+ extraskeletal cells regulate fracture healing and heterotopic ossificationBone ResJuly 6, 2026Ezgi Aydin, Jacob A. Moore, Mark Bubnovich, Gabriel Cuilan, Kristin Gebauer, Brenda Kim, Emily Chu, Ugur M. AyturkRRID:AB_394816), perilipin (Cell Signaling Technology Cat#9349, RRID:AB_10829911), Sox9 (R&D Systems, Cat#AF3075, RRID:AB_2194160) and/or laminin (Abcam, Cat#11575, RRID:AB_298179) (1:100 dilution) were then applied

(4) TGFβ signalling maintains Sox4+ astrocyte progenitors and restricts the early differentiation of BLBP+ astrocytes in the mouse dentate gyrusFrontiers in Cellular NeuroscienceJuly 1, 2026Kyoji Ohyama, Shoichiro Omura, Natsumi Takayama, Tokiharu TakahashiT. Edlund, 1:1,000); rabbit anti-Sox4 (Millipore, AB5803, 1:500–1,000); goat anti-Sox9 (R&D systems, AF3075, 1:1,000); rabbit anti-TGFβ1 (Santa cruz, sc-146, 1:100). Species-appropriate Alexa Fluor-conjugated

(5) Astrocyte-microglia crosstalk via CSF1 and IFN-β promotes central nervous system repairCell ReportsJune 23, 2026Tuoxin Cao, Matin Hemati-Gourabi, Yongliang Liu, Anna E. Mills, Lauren Baur, Ellie P. Rice, Theo Klippel, Yiwen Yan, Xiu Xu, William K. Fenske, et al.(rabbit, 1:2000, Dako 0334 or rat, 1:500, Invitrogen, 13–0300), SOX9 (goat, Biotechne-R&D Systems, AF3075), neurofilament 200 (rabbit, 1:300, Millipore Sigma, N4142-25UL), CD45 (rat, 1:300, BD Bioscience, 550539),

(6) L1CAM signaling through planar cell polarity drives SOX2 expression and lung adenocarcinoma metastasisNature CommunicationsJune 18, 2026Jin Suk Park, Yasemin Kaygusuz, Carson Kenum, Lan He, Mohamed I. Gatie, Xueqian Zhuang, Roshan Sharma, Ronan Chaligné, Umesh K. Bhanot, Richard P. Koche, et al.rabbit antibodies [Seven Hills Bioreagents Cat# WRAB-1231]; anti-SOX9 goat antibodies [R&D Systems Cat# AF3075]) diluted in PBS, 0.2% Triton-X, 0.001% heparin (PTwH), 3% donkey serum, and 5% DMSO. Samples were washed

(7) TGF-β signaling promotes astroglial activation and TDP-43 proteinopathy in organoid models of frontotemporal lobar degenerationJ Clin InvestJune 16, 2026Arren C. Ramsey, Xiao-Yan Tang, Magdalena J. Macias, Patricia R. Nano, Rufei Lu, Brian Benito, Cameron M. Lau, Jisu Park, Jiasheng Zhang, Wandy Beatty, et al.Invitrogen; RRID:AB_86543), anti-S100B (PA5-24996; Thermo Fisher Scientific; RRID:AB_2542496), anti-SOX9 (AF3075; R&D Systems; RRID:AB_2194160), anti-NES clone 10CS (MAB5326; Millipore; RRID:AB_2251134), anti-TDP-43

(8) Scg2 drives corticospinal circuit reorganization with spinal premotor interneurons and astrocytes for motor recovery after stroke in miceNat CommunJune 9, 2026Tokiharu Sato, Yuka Nakamura, Kana Hoshina, Ken-Ichi Inoue, Masahiko Takada, Masato Yano, Hitoshi Matsuzawa, Masaki Ueno(1:1000; ABN78, Merck), goat anti-Iba1 (1:200; NB100-1028, Novus Biologicals), goat anti-SOX9 (1:200; AF3075, R&D systems), rabbit anti-SCG2 (1:1000; MSRF105440, Nittobo Medical), rabbit anti-cFos (1:1000; 2250,

(9) ATM-kinase deficiency triggers early multi-compartment remodeling of the cerebellar microenvironmentActa Neuropathologica CommunicationsMay 29, 2026Francesca Montarolo, Luna Berrino, Anita Maria Rominto, Matilde Loddo, Ilaria Balbo, Anastasia Ricci, Giulia Pia Servetto, Ginevra Mango, Kenta Yamamoto, Roberta Parolisi, et al.Agilent, Santa Clara, CA, USA; 1:500, RRID: AB_10013382), SRY-box transcription factor 9 (SOX9; goat, AF3075, R&D system; 1:1000, RRID: AB_3750679), SRY-box transcription factor 10 (SOX10; rabbit polyclonal, HPA068898,

(10) Astrocyte glucocorticoid receptor signalling restricts neuronal plasticityNatureMay 20, 2026Bruno Gegenhuber, Takuma Sonoda, Lisa Traunmüller, Christopher P. Davis, Shon A. Koren, Eric C. Griffith, Chinfei Chen, Michael E. Greenbergbuffer as follows: rabbit anti-GR (Cell Signaling Technology 12041S, 1:300), goat anti-SOX9 (R&D Systems AF3075, 1:300), chicken anti-GFP (Aves Labs GFP-1020, 1:1,000), rabbit anti-Acan (Sigma AB1031, 1:500), guinea pig

(11) Pericytes are organ-specific regulators of tissue morphogenesisNat CommunMay 12, 2026Seyed Javad Rasouli, Kai Kruse, Rodrigo Diéguez-Hurtado, Parisa Ghanbari, Anusha Aravamudhan, Mara E. Pitulescu, Ralf H. Adams(1:100, Millipore; AB769), Rabbit anti-SOX2 (1:100, Abcam, ab97959), goat anti-SOX9 (1:100, R&D Systems, AF3075), rat anti-Nestin (1:100, Cosmo Bio, BAM-73-100-EX), rabbit anti-FOSB (1:100, Cell Signaling, #2251),

(12) Tracing motor neurons and primary sensory afferents of the monkey spinal cord with cholera toxin subunit BNeural Regeneration ResearchMay 1, 2026Ziyu He, Zhixian Liu, Wenjie Xu, Ruoying Zhang, Shu Fan, Wei Wang, Xiaolong Zheng,Species/dilution,Supplier,Cat#/RRID SOX9,Goat/1:200,"R&D Systems, Minneapolis, MN, USA",AF3075/AB_2194160 GFAP,Rabbit/1:1000,"Cell Signaling Technology, Danvers, MA, USA",12389/AB_2631098 OLIG2,Goat/1:200,R&D Systems

(13) TAR syndrome causal gene RBM8A is critical for embryonic bone development and proper Hedgehog signalingbioRxivApril 27, 2026Jennifer Mott, Zifei Pei, Jiawan Sun, Choong Heon Lee, Twishi Puri, Zander M. Sachs, Ana Aviles Aviles Vargas, Nicholas Blaha, Mabel Tong, Aimin Liu, et al.MNCD2-s), rabbit anti-Ki67 (Invitrogen, catalog #: 701198), or goat anti-SOX9 (R&D Systems, catalog #: AF3075). After staining, coverslips were mounted onto microscope slides using ProLong Diamond Antifade Mountant with DAPI

(14) CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegenerationNature CommunicationsApril 22, 2026Ioana I. N. Da Silva, Desiree Ramirez, Sarah L. Parylak, Leslie A. Wallace, James K. Tucker, Joel M. Erberich, Rutvi Katariya, Aidan H. McDonald, Iryna S. Gallina, Ariana L. Tucker, et al.used: Chicken anti-GFP (1:300; Aves Labs: GFP-1020), Goat polyclonal anti-hSox9 (1:250; R&D Biosystems: AF3075), Rabbit monoclonal anti- NF-κB p65/RelA(1:400; CTS: 8242), Mouse monoclonal anti- Phospho-RelA/NFkB p65 (S529

(15) Hippo signaling differentially regulates distal progenitor subpopulations and their transitional states to construct the mammalian lungsNature CommunicationsApril 3, 2026Kuan Zhang, Madhuri Basak, Youssef Zaher, Erica Yao, Shao-An Wang, Thin Aung, Pao-Tien ChuangCell Signaling Technology, Cat# 23064S; RRID:AB_2714146), goat anti-SOX9 (1:200, R&D Systems, Cat# AF3075; RRID:AB_2194160). Immunofluorescence and Immunohistochemistry Dissected mouse lungs were fixed in

(16) Glycogen and lactate metabolism in mouse fetal Sertoli cells sustain the germ lineCell ReportsMarch 24, 2026Martín A. Estermann, Joseph Sheheen, Sara A. Grimm, Boris Tezak, Yu-Ying Chen, Tsuyoshi Morita, Humphrey H.-C. H.-C. Yao, Blanche CapelAB_2918304 PYGB,Proteintech,Cat# 12075-1-AP; RRID: AB_2174885 SOX9,Milipore,Cat# AB5535; RRID: AB_2239761 SOX9,R&D systems,Cat# AF3075; RRID: AB_2194160 SRY,Dagmar Williams,RRID: AB_2687957 Ki-67,BD,Cat# 550609; RRID: AB_393778 CC3,Cell Signaling

(17) Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivityeLifeMarch 9, 2026Kawanabe-Kobayashi R, Uchiyama S, Yoshihara K, Koga K, Kojima D, McHugh TJ, Hatada I, Matsui K, Tanaka KF, Tsuda M.#13-0300; Invitrogen); polyclonal goat anti-SRY-related high-mobility group box 9 (SOX9; 1:1000; # AF3075; R&D Systems); polyclonal goat anti-paired box 2 (PAX2; 1:500; # AF3364; R&D Systems); monoclonal rat

(18) Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivityeLifeMarch 9, 2026Riku Kawanabe-Kobayashi, Sawako Uchiyama, Kohei Yoshihara, Keisuke Koga, Daiki Kojima, Thomas J McHugh, Izuho Hatada, Ko Matsui, Kenji F Tanaka, Makoto Tsuda#13-0300; Invitrogen); polyclonal goat anti-SRY-related high-mobility group box 9 (SOX9; 1:1000; # AF3075; R&D Systems); polyclonal goat anti-paired box 2 (PAX2; 1:500; # AF3364; R&D Systems); monoclonal rat

(19) Hippo signaling differentially regulates distal progenitor subpopulations and their transitional states to construct the mammalian lungsbioRxiv : the preprint server for biologyMarch 8, 2026Zhang K, Basak M, Zaher Y, Yao E, Wang SA, Aung T, Chuang PT.

(20) Flexibility of cell fates and functions across sex determination systems revealed by comparative single-cell analysesbioRxivJanuary 31, 2026Rafael D. Acemel, Boris M. Tezak, Vicky Wai Yee Chung, Alicia Hurtado, Scarlett Albertson, Ceri J. Weber, Blanche Capel, Darío G. Lupiáñez,Santa Cruz,SC-1237 Gata4,Mouse,1:500,Santa Cruz,Sc-25310 HuC/ HuD,Mouse,1:50,Thermo Fisher,A-21271 Sox9,Goat,1:500,R&D Systems,AF3075 Lhx9,Goat,1:200,Santa Cruz,SC-19348 Twist1,Rabbit,1:100,Cell Signaling,90445 CTNNB1,Mouse,1:200,Sigma Aldrich

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2/1/2017Detect Sox9 In Mouse Embryonic Stem Cells Sadaf AtarodThe aim of this experiment was to detect the expression of Sox9 in fixed mouse embryonic stem cells. The goal was to observe Sox9 positive-stained cells. The specific reason for selecting this ... Read Review

Human SOX9 Antibody

SOX9 Antibody

SOX9 Antibody

SOX9 Antibody

SOX9 Antibody

SOX9 Antibody from R&D Systems, a Bio-Techne Brand

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Questions and Answers

R&D Systems, a Bio-Techne Brand

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SOX9 Antibody from R&D Systems, a Bio-Techne Brand

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