KLF4 Antibody AF3158 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemKLF4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3158
ApplicationsCo-Immunoprecipitation,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Immunocytochemistry/ Immunofluorescence,In Situ Hybridization
TargetKLF4
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse KLF4, Ala11-Phe483, Accession # Q60793
NCBI Full Gene NameKLF transcription factor 4, Kruppel-like transcription factor 4 (gut)
NCBI Gene AliasesGKLF, Gklf, EZF, Zie
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse KLF4 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this KLF4 antibody be used for ChIP assays in mouse embryonic stem cells?

Yes, the KLF4 antibody has been utilized in chromatin immunoprecipitation (ChIP) assays, as indicated in the literature where it was employed to study transcription factor binding in mouse embryonic stem cells ().

What applications has this antibody been shown to work in?

The KLF4 antibody is suitable for various applications including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation, as supported by multiple studies (, ).

Is this antibody effective for detecting KLF4 in human samples?

Yes, the KLF4 antibody has been shown to react with both mouse and human KLF4, making it applicable for studies involving human samples ().

How has this antibody been utilized in studies related to pluripotency?

The KLF4 antibody has been used in research focusing on pluripotency factors, including its role in the reprogramming of somatic cells to pluripotent states, as demonstrated in studies examining the interactions of KLF4 with other transcription factors like Sox2 ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Novel Netrin-1-Unc5b-Klf4 axis balances AT2 survival in experimental bronchopulmonary dysplasiaAmerican Journal of Respiratory Cell and Molecular BiologySeptember 1, 2026Oleksiy Klymenko, Jasmine Mohr, Soula Danopoulos, Ivana Mižík, Celien Kuiper-Makris, Stephanie Stephan, Mohammad A Mahjoub, Cristina M Alvira, Daniela Rheindorf, Thomas Imhof, et al.and IF staining for anti-SFTPC (#WRAB-9337, Seven Hills Bioreagents) was combined with anti-KLF4 (#AF3158, R&D Systems). The KLF4 signal was amplified with the Alexa Fluor 594 Tyramide SuperBoost Kit, goat

(2) Oncogenic Ras drives EED degradation and PRC2 dysfunction to promote aggressive squamous cell carcinomaNature CommunicationsJuly 13, 2026Meng-Yen Li, Aubrey Houser, Pradeep Cheema, Xiang Yu Zheng, Paula Restrepo, Pooja Flora, Yudong Zhou, Gil Segal-Dalal, Eldad Silberstein, Xiaotao Zhang, et al.BioLegend, 905901), chicken anti-KRT14 (1:500, BioLegend, 906001), mouse anti-KLF4 (1:250, R&D systems, AF3158), rabbit anti-KRT17 (1:500, Abcam, 109725), rabbit anti-LEPR (1:200, Sigma-Aldrich, HPA030899), rabbit

(3) Promoter reinforcement supports transcriptional resilience in drug-resistant cancerNat Struct Mol BiolJuly 6, 2026Vasumathi Kameswaran, Sayantanee Paul, Daniel Le, Alissa D. Guarnaccia, Jonathan Hoover, Liang-Fu Chen, Thijs J. Hagenbeek, Minyi Shi, Luke Y. Zhao, Jessica M. Lund, et al.antibodies against FOSL1 (PA5-66880, Invitrogen; 5 μg), YAP (14074S, Cell Signaling; 2 μg) and KLF4 (AF3158, R&D Systems; 5 μg). Antibody chromatin complexes were pulled down with Protein A magnetic beads and

(4) KLF4 Promotes a KRT13+ Hillock-Like State in Lung Squamous Cell CarcinomaCancer ResJune 18, 2026Luke T. Izzo, Tony Reyes, Srijan Meesala, Abbie S. Ireland, Lisa B. Earnest-Noble, Steven Yang, Hari Shankar Sunil, Xiao Chun Cheng, Nomi Tserentsoodol, Sarah B. Hawgood, et al.beads (EpiCypher Cat # 21-1401), and subject to antibody binding overnight (anti-KLF4, R&D Systems AF3158, RRID: AB_2130245, 0.5 μg; IgG, CST 2729S, RRID: AB_1031062, 0.5 μg). pAG-MNase was used to digest the

(5) Targeted neuronal reprogramming rescues memory and neural synchrony in Alzheimer's diseaseMol BiomedJune 10, 2026Marcos Galán-Ganga, Irene Rodríguez-Navarro, Sofía Zaballa, Alba Ramón-Lainez, Nerea Gómez-Rivada, Christian Peters, Joaquín Fernández-Irigoyen, Enrique Santamaría, M Ángeles Rabadán, Jordi Alberch, et al.1:200, ab1793, Abcam), anti‑NANOG (rabbit, 1:300, NB100‑58,842, Novus), and anti‑Klf4 (goat, 1:300, #AF3158, R&D Systems). Imaging was performed using a Leica TCS SP5 confocal microscope with a 40 × objective

(6) A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitroDevelopmental CellDecember 15, 2025Daniel Medina-Cano, Mohammed T. Islam, Veronika Petrova, Sanjana Dixit, Zerina Balic, Marty G. Yang, Matthias Stadtfeld, Emily S. Wong, Thomas VierbuchenRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit pAb anti-GFP,Abcam,Cat#AB290;RRID:AB_303395 Goat pAb anti-KLF4,R&D Systems,Cat#AF3158;RRID:AB_2130245 Rabbit mAb anti-NANOG (D2A3),Cell Signaling Technology,Cat#8822S;RRID:AB_11217637 Mouse

(7) KRT6A and KRT17 Mark Distinct Stem Cell Populations in the Adult Palpebral Conjunctiva and Meibomian GlandCellsDecember 12, 2025Xuming Zhu, Mingang Xu, David M. Owens, Sarah E. MillarUSA, 5292S, 1:200), mouse anti-MYC-tag (Cell Signaling, 2276S, 1:1000); goat anti-KLF4 (R&D Systems, AF3158, 1:100); chicken anti-KRT14 (BioLegend, 906004, 1:500); rat anti-Ki-67 (eBioscience, San Diego, California,

(8) Peptide Family Promotes Brain Cell Rejuvenation and Improved Cognition through Peripheral DeliveryACS OmegaApril 8, 2025Alejandro Anton-Fernandez, Indalo Domene-Serrano, Raquel Cuadros, Rocio Peinado-Cahuchola, Margarita Sanchez-Pece, Felix Hernandez, Jesus Avilaantibody in PBSM. The following primary antibody dilutions were used: anti-Klf4 (1/1000; R&D System, ref: AF3158) and anti-Sox2 (1/1000; R&D Systems, ref: AF2018) for total cell lysate. β-actin (1/5000; SIGMA, ref:

(9) Hippocampal rejuvenation by a single intracerebral injection of one-carbon metabolites in C57BL6 old wild-type miceAging cellJanuary 1, 2025Antón-Fernández A, Cauchola RP, Hernández F, Ávila J.ref: PA5‐101588) for the nuclear fraction, anti‐Krüppel‐like factor 4 (Klf4, 1/500; R&D system, ref: af3158) for the cytosolic fraction, and Lamin B1 (1/1000; Santa Cruz SC‐377000) and β‐actin (1/10000; SIGMA,

(10) Metabolic regulator ERRγ governs gastric stem cell differentiation into acid-secreting parietal cellsCell Stem CellJune 6, 2024Mahliyah Adkins-Threats, Sumimasa Arimura, Yang-Zhe Huang, Margarita Divenko, Sarah To, Heather Mao, Yongji Zeng, Jenie Y. Hwang, Joseph R. Burclaff, Shilpa Jain, et al.conjugated GSII lectin; Alexa Fluor 647,Invitrogen,Cat# L32451 goat polyclonal anti-KLF4,R&D systems,Cat# AF3158; RRID:AB_2130245 Mouse monoclonal anti-Muc5ac,Abcam,Cat# ab3649; RRID: AB_2146844 Phalloidin-iFluor 647 Conjugate

(11) Hypoxia Increases the Efficiencies of Cellular Reprogramming and Oncogenic Transformation in Human Blood Cell Subpopulations In Vitro and In VivoCellsJune 4, 2024Adrián Moratilla, Diana Martín, Marta Cadenas-Martín, Martha Stokking, Maria Angustias Quesada, Francisco Arnalich, Maria P. De Miguelcontrols. Anti-Oct3/4 (sc-9081, Santa Cruz Biotechnology) was employed at a 1:350 dilution, anti-Klf4 (AF3158, R&D Systems, Minneapolis, MN, USA) at a 1:100 dilution, anti-LIFR (sc-659, Santa Cruz Biotechnology)

(12) In vivo cyclic overexpression of Yamanaka factors restricted to neurons reverses age-associated phenotypes and enhances memory performanceCommunications BiologyMay 24, 2024Alejandro Antón-Fernández, Marta Roldán-Lázaro, Laura Vallés-Saiz, Jesús Ávila, Félix HernándezDoublecortin (1:250, Merck, ab2253), mouse H4K20me3 (1:200, Abcam, ab78517), goat Klf4 (1:250, R&D system, af3158), rabbit Tbr2 (1:200, Abcam, ab23345). Secondary antibodies were used as follows: donkey anti-goat Alexa-488

(13) Role of endothelial PDGFB in arterio-venous malformations pathogenesisAngiogenesisMay 1, 2024Yanzhu Lin, Johannes Gahn, Kuheli Banerjee, Gergana Dobreva, Mahak Singhal, Alexandre Dubrac, Roxana OlaLife Technologies), anti-GOLPH4 (#ab28049; 1:200, Abcam), anti-ERG (#92513; 1:200, Abcam), anti-KLF4 (#AF3158, 1:200, R&D systems), anti-SOX17 (#AF1924, 1:200, R&D systems), anti-VE-cadherin (#555289, 1:400, BD),

(14) The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomesNature geneticsJanuary 1, 2024Brahma S, Henikoff S.(Bethyl Laboratories cat. no. A300-397A, lot no. 3), 1:100; KLF4: goat polyclonal (R&D Systems cat. no. AF3158, lot. no. WRR0719011), 1:100 for CUT&RUN, 1:50 for immunofluorescence; OCT4: rabbit monoclonal (EPR17929,

(15) A reversible epigenetic memory of inflammatory injury controls lineage plasticity and tumor initiation in the mouse pancreasDevelopmental CellDecember 5, 2023David J. Falvo, Adrien Grimont, Paul Zumbo, William B. Fall, Julie L. Yang, Alexa Osterhoudt, Grace Pan, Andre F. Rendeiro, Yinuo Meng, John E. Wilkinson, et al.anti-F4/80,Cell Signaling Technology,"Cat# 70076, RRID:AB_2799771" Goat Polyclonal anti-KLF4,R&D Systems,"Cat# AF3158, RRID:AB_2130245" Goat Polyclonal anti-KLF5,R&D Systems,"Cat# AF3758, RRID:AB_2130246" Rabbit Polyclonal

(16) Trained immunity of alveolar macrophages enhances injury resolution via KLF4-MERTK-mediated efferocytosisThe Journal of experimental medicineNovember 6, 2023Chakraborty S, Singh A, Wang L, Wang X, Sanborn MA, Ye Z, Maienschein-Cline M, Mukhopadhyay A, Ganesh BB, Malik AB, et al.PBS/Tw-20 for 1 h at room temperature. Afterward, cells were incubated with anti-KLF4 primary antibodies (#AF3158; R & D system) and incubated overnight at 4°C. The next day, slides were washed and incubated with the

(17) SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformationsThe Journal of clinical investigationSeptember 15, 2023Banerjee K, Lin Y, Gahn J, Cordero J, Gupta P, Mohamed I, Graupera M, Dobreva G, Schwartz MA, Ola R.anti-ERG (Abcam no. 92513; 1:200), anti-ERG-647 (Abcam no. 196149; 1:100), anti-KLF4 (R&D Systems no. AF3158; 1:200), anti-phospho S6 (pS6; Cell Signaling no. 5364; 1:200), anti-SOX17 (R&D Systems no. AF1924;

(18) The circadian clock CRY1 regulates pluripotent stem cell identity and somatic cell reprogrammingCell reportsJune 27, 2023Sato S, Hishida T, Kinouchi K, Hatanaka F, Li Y, Nguyen Q, Chen Y, Wang PH, Kessenbrock K, Li W, et al.

(19) Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers cerebral cavernous malformationJCI insightMarch 8, 2023Yang X, Wu ST, Gao R, Wang R, Wang Y, Dong Z, Wang L, Qi C, Wang X, Schmitz ML, et al.

(20) RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomesbioRxiv : the preprint server for biologyJanuary 22, 2023Brahma S, Henikoff S.(Bethyl Laboratories cat. no. A300–397A, lot no. 3), 1:100; KLF4: goat polyclonal (R&D Systems cat. no. AF3158, lot. no. WRR0719011), 1:100 for CUT&RUN, 1:50 for immunofluorescence; OCT4: rabbit monoclonal (abcam

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1/4/2018KLF4 Antibody Amy CWas trying to knock out KLF4 in ESCs and needed to verify knock out by Western. Used this antibody and it was very clean. It had one prominent band corresponding to the size of the KLF4, and the ... Read Review

KLF4 Antibody

KLF4 Antibody

KLF4 Antibody

KLF4 Antibody

KLF4 Antibody

KLF4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (106)

Reviews

KLF4 Antibody from R&D Systems, a Bio-Techne Brand

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