KLF4 Antibody

January 04, 2018

Urology
University of California, San Francisco
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

KLF4 antibody

Catalog Number:

af3158

Was trying to knock out KLF4 in ESCs and needed to verify knock out by Western. Used this antibody and it was very clean. It had one prominent band corresponding to the size of the KLF4, and the knockout line had no band.

Experimental Design and Results Summary

Applications

Western Blot

Sample

ESC lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

Odyssey blocking buffer

Secondary Incubation

1:10000 licor secondary, 1 hr at room temp

Tertiary Incubation

None

Detection

Infrared (Licor)

Results Summary

Clean, specific bands

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean specific band

The Bad

None

The Bottom Line

Great antibody for western

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