Integrin beta 1/CD29 Antibody MAB17781 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIntegrin beta 1/CD29 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberMAB17781
ApplicationsBioassay,Blocking,Flow Cytometery,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunofluorescence,Immunocytochemistry,CyTOF-ready, ELISA Capture
ReactivityHuman,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Papio cynocephalus (Yellow Baboon)
TargetIntegrin beta 1/CD29
CloneP5D2
HostMouse
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
IsotypeIgG1
ClonalityMonoclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenHuman skin keratinocytes
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameintegrin subunit beta 1
NCBI Gene AliasesCD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Integrin ß1/CD29.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to block integrin β1 in cell adhesion studies?

Yes, the Integrin beta 1/CD29 Antibody has been shown to effectively block integrin β1 activity in various studies. For instance, in a study investigating monocyte adhesion, the antibody was used at a concentration of 10 mg/ml to inhibit THP-1 cell adhesion to VCAM-1, demonstrating its utility in cell adhesion assays ().

Is this antibody suitable for use in flow cytometry applications?

The Integrin beta 1/CD29 Antibody has been utilized in flow cytometry for identifying integrin expression in human dental pulp stem cells, confirming its applicability in this technique ().

What experimental setups have utilized this antibody for studying cancer cell behavior?

This antibody has been employed in studies examining the role of integrin β1 in cancer cell invasion and response to therapies. For example, it was used to assess the impact of integrin β1 signaling on pancreatic cancer cells in coculture assays, where blocking this integrin altered the radioprotection of cancer cells ().

Does this antibody have any documented applications in 3D culture systems?

Yes, the Integrin beta 1/CD29 Antibody has been referenced in studies involving 3D culture systems, where it was used to block integrin β1 signaling, thereby influencing the behavior of carcinoma cell aggregates in a microfluidic environment ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Tissue engineering polymeric formulations enable lymphangiogenesis for myocardial repairBioact MaterOctober 12, 2026Zhuo Liu, Ruizhi Li, Wenxin Huang, Jinyi Zhang, Ying Qu, Wenke Dong, Yonghang Liu, Qingze Li, Xiaoyang Lin, Huitang Xia, et al.adhesion assay 96-well plates were coated with recombinant human Tenascin-C (15 μg/mL; R&D Systems, MAB17781) overnight at 4 °C and then blocked with 1% BSA for 1 h. LECs were pre-treated with 3,4-DHPLA (50 μM)

(2) Hepatokine FGL1 drives endothelial injury and atherogenesis via integrin β1-mediated endothelial–mesenchymal transitionActa Pharmaceutica Sinica BJune 8, 2026Yixiu Zhao, Huan Chen, Liuqing Yin, Ruirui Meng, Jiangfei Zheng, Yue Zhang, Zhiqi Wang, Yi Chen, Rui Xu, Jing Liu, et al.(IgG) served as controls. The intravenous injection of integrin β1 antibody (15 μg/kg, R&D Systems, MAB17781, once weekly) and RGD peptide (Gly–Arg–Gly–Asp–Asn–Pro, GRGDNP, 5 mg/kg, MCE, HY-P1740A, twice weekly)

(3) Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal AdenocarcinomaAdvanced ScienceNovember 1, 2025Shan Zhang, Luju Jiang, Shuqi Cai, Zheqi Weng, Yuheng Zhu, Zhi‐Wei Cai, Hui Li, Qing Li, Li‐Peng Hu, Hong‐Fei Yao, et al.indicated groups, recombinant human GC protein (50 ng mL−1) or anti‐ITGB1 (5 µg mL−1, R&D systems, Cat. MAB17781), or defactinib (5 µm, Selleck, Cat. S7654) was added into the medium. The neural invasion index was

(4) CYR61 Expression Is Induced by IGF1 and Promotes the Proliferation of Prostate Cancer Cells Through the PI3/AKT Signaling PathwayInternational Journal of Molecular SciencesSeptember 15, 2025Greisha L. Ortiz-Hernández, Carmina Patrick, Stefan Hinz, Mark A. LaBarge, Yun R. Li, Susan L. NeuhausenDallas, TX, USA, Cat# sc-374129), Beta 1 (β1) Integrin (R&D Systems Inc., Minneapolis, MN, USA, Cat# MAB17781), and β1 Integrin (Developmental Studies Hybridoma Bank, Iowa City, IA, USA, Cat# P5D2). 4.3. Immunoblotting Whole-cell

(5) Negative regulation of lymphangiogenesis by Tenascin-C delays the resolution of inflammationiScienceFebruary 21, 2025Daisuke Katoh, Yoshiyuki Senga, Kento Mizutani, Kazuaki Maruyama, Daishi Yamakawa, Tadashi Yamamuro, Michiaki Hiroe, Keiichi Yamanaka, Akihiro Sudo, Naoyuki Katayama, et al.anti-integrin β1 (12G10),Abcam,Ab30394; RRID:AB_775726 Mouse monoclonal anti-integrin β1 (P5D2),R&D system,MAB17781; RRID:AB_2129940 Mouse monoclonal anti-α-tubulin (DM1A),Cedarlane,CTL9002; RRID:AB_3665220 Rabbit

(6) Fitness Screens Map State-Specific Glioblastoma Stem Cell VulnerabilitiesCancer researchDecember 2, 2024MacLeod G, Molaei F, Haider S, Almeida MP, Lin S, Kushida M, Sureshkumar H, Bhatti JK, Lu JQ, Schramek D, et al.

(7) Infection of human organoids supports an intestinal niche for Chlamydia trachomatisPLoS pathogensAugust 1, 2024Hovhannisyan P, Stelzner K, Keicher M, Paprotka K, Neyazi M, Pauzuolis M, Ali WM, Rajeeve K, Bartfeld S, Rudel T.surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 μg/ml, R&D Systems, MAB17781) for 1h at 37°C, followed by infection with C. trachomatis L2 at MOI of 0.5 in the presence of antibodies.

(8) Defining the mechanism of galectin-3-mediated TGF-β1 activation and its role in lung fibrosisThe Journal of biological chemistryJune 1, 2024Calver JF, Parmar NR, Harris G, Lithgo RM, Stylianou P, Zetterberg FR, Gooptu B, Mackinnon AC, Carr SB, Borthwick LA, et al.

(9) β1-Integrin-Mediated Uptake of Chondrocyte Extracellular Vesicles Regulates Chondrocyte HomeostasisInternational journal of molecular sciencesApril 26, 2024Hussain MT, Austin-Williams S, Wright TD, Dhawan UK, Pinto AL, Cooper D, Norling LV.

(10) Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote MetastasisCancer ResearchApril 1, 2024Ying Liu, Ileana Murazzi, Ashley M. Fuller, Hehai Pan, Valerie M. Irizarry-Negron, Ann Devine, Rohan Katti, Nicolas Skuli, Gabrielle E. Ciotti, Koreana Pak, et al.Sigma, 25322-68-3). Integrin β1 stimulating antibody and IgG control were purchased from R&D Systems (MAB17781(RRID:AB_2129940) and MAB002 (RRID:AB_357344) respectively). Recombinant human PLOD2 protein was a kind

(11) Sarcoma cells secrete hypoxia-modified collagen VI to weaken the lung endothelial barrier and promote metastasisCancer ResApril 1, 2024Ying Liu, Ileana Murazzi, Ashley M. Fuller, Hehai Pan, Valerie M Irizarry-Negron, Ann Devine, Rohan Katti, Nicolas Skuli, Gabrielle E. Ciotti, Koreana Pak, et al.Sigma, 25322-68-3). Integrin β1 stimulating antibody and IgG control were purchased from R&D Systems (MAB17781(RRID:AB_2129940) and MAB002 (RRID:AB_357344) respectively). Recombinant human PLOD2 protein was a kind

(12) Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein AnalysisACS sensorsDecember 23, 2022Martel R, Shen ML, DeCorwin-Martin P, de Araujo LOF, Juncker D.CD9-BC51, ITG α6 (MAB1350, R&D Systems)-BC09, and Alix (MA1-83977, Invitrogen)-BC60; CD81-BC50, ITG β1 (MAB17781, R&D Systems)-BC27, and HSP90-BC60; CD82 (342102, Biolegend)-BC43, ITG β4 (MAB4060, R&D Systems)-BC79,

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(14) MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin PathwayInternational journal of stem cellsNovember 30, 2021Li Q, Huang L.antibodies for 25 min at 4℃: CD44 (ab6124, Abcam, Cambridge, MA, USA), CD73 (ab202122, Abcam, USA), CD29 (MAB17781, R&D System, Minneapolis, MN, USA), CD31 (ab9498, Abcam, USA), CD34 (ab81289, Abcam, USA), and CD45

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(16) Irisin supports integrin-mediated cell adhesion of lymphocytesBiochemistry and biophysics reportsJuly 1, 2021Myint PK, Ito A, Appiah MG, Obeng G, Darkwah S, Kawamoto E, Gaowa A, Park EJ, Shimaoka M.

(17) In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cellsNature communicationsJuly 14, 2020Luthria G, Li R, Wang S, Prytyskach M, Kohler RH, Lauffenburger DA, Mitchison TJ, Weissleder R, Miller MA.

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(20) Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4⁺ T Cell Engulfment ProcessCell reportsNovember 5, 2019Davies SP, Reynolds GM, Wilkinson AL, Li X, Rose R, Leekha M, Liu YS, Gandhi R, Buckroyd E, Grove J, et al.(Biolegend, 324201), anti-β2 integrin (Novus Biologicals, AF1730), anti-β1 integrin (Novus Biologicals, MAB17781), Cells were incubated with primary antibody for one hour at room temperature (20-22°C), or 24 hours

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8/9/2018Excellent Antibody to Precipitate Human Beta 1 IntegrinDr. Elena DeryuginaIntegrins are cell surface transmembrane molecules that primarily mediate adhesion of cells to matrix proteins and induce cell signaling. Among integrins, beta 1 integrins play crucial roles in cell ... Read Review