Fig 1: (A) Schematic representation of the promoter-Ruby construct T-DNAs. The codA-nptII selection marker gene is under the control of the Arabidopsis Ubiquitin10 promoter (Ubi10p) and nopaline synthase transcriptional terminator (T). The test promoters from citrus (CitWaxp, CitUNKp, and CitVO1p) plum (PamMybAp) and tomato (SlE8p) are shown upstream of the Ruby (CsmybA1) gene. LB and RB designate the Agrobacterium left and right borders. Blue arrows indicate the approximate position of the codA and Ruby primers used for molecular validation. (B) PCR confirmation of the presence of codA transgene in 10 independent events. (C) PCR confirmation of the presence of the CitWaxp-Ruby sequence. P designates a sample with a plasmid DNA template. N indicates genomic DNA from wildtype Mexican lime. MW indicates molecular marker (Promega, 1 kb DNA ladder, G7541).
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Fragment Sizes:250, 253, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000 bp