Science Leader for Nucleic Acid Innovation
National Measurement Laboratory at LGC
For simple and absolute nucleic acid quantification, scientists in a variety of fields are turning to digital PCR (dPCR). By partitioning samples prior to amplification, dPCR allows for the detection of even rare events in a sample and makes multiplexing easy.
Scientists must carefully craft their dPCR experiments and report their protocols. The dMIQE (Minimum Information for Publication of Quantitative Digital PCR Experiments) guidelines were developed to provide a practical framework that supports researchers using digital PCR (dPCR) from experimental design through to publication. Their aim is to strengthen the scientific field by promoting robust, reproducible, and transparently reported research.
In this webinar, brought to you by Bio-Rad, Alexandra Whale, a co-author of the dMIQE guidelines, will explain the principles behind the guidelines and demonstrate how they can be applied in practice using a multiplex dPCR experiment as a worked example. She will explore key aspects of multiplex assay design and optimization, as well as approaches for visualizing and interpreting complex dPCR scatter plots. Through this example, attendees will gain practical insights into generating, analyzing, and reporting multiplex dPCR data to support accurate, reliable, and reproducible results.
Whether you are new to dPCR or are looking to take your experiments to the next level, this event will guide you toward rigorous and publication-ready experiments.
Topics to be covered in this webinar
- Planning and executing dPCR experiments
- Utilizing the dMIQE guidelines
- Real-world examples of multiplexing with dPCR
- Reporting dPCR results
- Comparison of dPCR technologies, including Droplet Digital PCR (ddPCR)
The content presented in these sessions is intended for research use only and is not intended for use in diagnostic procedures. The views and opinions expressed in the recordings are those of the individual presenters and do not necessarily reflect the official policy, position, or claims of Bio-Rad Laboratories.
Any clinical applications, test validations, or performance claims discussed are the sole responsibility of the presenting institution or speaker and do not reflect the views or endorsements of Bio-Rad Laboratories.
Any reference to clinical use, patient testing, or performance claims reflects the practices and experience of the individual institutions or researchers and should not be interpreted as endorsement by Bio-Rad.
For Research Use Only. Not for use in diagnostic procedures.