Spectral Flow Cytometers

Spectral Flow Cytometers Spectral flow cytometers capture the full fluorescence spectrum of labeled cells and particles, unlike conventional cytometers, which rely on optical filters to isolate specific wavelengths. Multiple specialized detectors enable the identification of multiple fluorophores simultaneously based on unique spectral fingerprints. Spectral unmixing, analogous to compensation in conventional cytometry, is used to distinguish fluorophores with even closely overlapping emission spectra. As a result, spectral cytometers can accommodate larger, more complex panels, enhancing multiparametric analysis. Selecting a spectral cytometer requires balancing cost with experimental needs, considering factors such as laser count, detector sensitivity, sample format, acquisition speed, and throughput. These specifications directly impact the resolution and efficiency of high-parameter experiments. Consult the instrument manufacturer for more specifications and features.

Spectral Flow Cytometers

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FP7000 Spectral Cell Sorter

FP7000 Spectral Cell Sorter

Sony Corporation

  • Excitation Lasers:
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  • Detection:
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ID7000&#8482; Spectral Cell Analyzer
  • Excitation Lasers:
    320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
  • Detection:
    32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
  • Sample Type:
    5-mL (12 x 75-mm) tubes, 96-well and 384-well plates (Autosampler)
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SA3800 Spectral Analyzer

SA3800 Spectral Analyzer

Sony Corporation

  • Excitation Lasers:
    405 nm, 488 nm, 561nm, 638 nm
  • Detection:
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  • Sample Type:
    96-well plates: standard, half deep, and deep; 384 standard flat, and 12 x 75-mm 5-mL tubes
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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer

Sony Corporation

  • Excitation Lasers:
    405 nm, 488 nm, 638 nm (2- or 3-laser configurations)
  • Detection:
    FSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
  • Sample Type:
    5-mL tubes
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Sony Corporation
ID7000
5
8 Figures from 319 Publications
Sony Corporation
SA3800
4
7 Figures from 311 Publications
Sony Corporation
SP6800
2
2 Figures from 186 Publications
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