Hydrolases are a class of enzymes that catalyze the hydrolysis of chemical bonds using water molecules. This broad enzyme category functions in a wide range of biological processes and is classified based on the type of bond they act upon. Proteases, for example, cleave peptide bonds and are responsible for protein turnover, digestion, blood clotting, apoptosis, and extracellular reorganization. Protein phosphatases oppose kinases by removing phosphate groups, making them essential to signal transduction pathways. Lipases, classified as a type of esterase, hydrolyze triglycerides and function in pathways ranging from fat metabolism to signaling. In addition to essential roles in breaking down complex molecules, hydrolases are versatile enzymes with broad applications across medicine and diagnostics, serving as biomarkers and therapeutic targets for disease. A variety of assays have been developed to measure the activity of both broad and specific hydrolase enzyme targets. These enzyme assay kits, which contain ready-to-use reagent sets, offer a convenient and versatile toolset for protein and biomedical research.
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We used the PureLink RNA Mini Kit to isolate total RNA from immunomagnetically purified adult mouse astrocytes for quantitative real-time PCR analysis of genes involved in mitochondrial glutamate transport and astrocyte metabolism. The kit ...
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We used the Lactate-Glo Assay Kit to quantify lactate concentrations in cultured primary astrocytes, mouse prefrontal cortex, cerebrospinal fluid, and serum while investigating astrocyte-specific regulation of brain energy metabolism following Npas3 ...
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