Ion exchange HPLC columns are packed with stationary phase particles that can trap and release ionic analytes. Anion exchange columns exchange negatively charged molecules while cation exchange columns exchange positively charged molecules. Common ion exchange media include diethyl aminoethyl (DEAE), carboxymethyl (CM), quaternary ammonium (Q), sulfonate (S), and sulfopropyl (SP). Ion exchange HPLC, which can operate under high pressure, allows for fast, high-resolution separation of proteins and other biomolecules. Consider column length, diameter, and particle size when looking for HPLC columns.
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Phenomenex
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- Hydrophilic Interaction, Ion Exchange, Normal Phase, Reversed Phase
Bio-Rad
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- Affinity Chromatography, Ion Exchange, Mixed Mode, Size Exclusion
MilliporeSigma
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- Anion Exchange, Cation Exchange, Ion Exchange
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- Anion Exchange, Ion Exchange
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- Cation Exchange, Ion Exchange
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