Luciferase assay kits are available from several suppliers to quantify the enzymatic activity of the luciferases. Luciferases are a class of oxidative enzymes that produce luminescence. Notable for their expression in fireflies, luciferase has become widely applied in research as a reporter for gene expression. Luciferase-based assays are also used in measuring cellular ATP levels when studying cell viability, metabolism, or kinase activity. Luciferase assays generally incorporate a luciferase substrate that reacts with luciferase to produce light, which can be measured by a luminometer. The amount of light generated is proportional to the activity of luciferase. Visit the luciferase assay kit suppliers for more details, including kit components.
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