Luciferase Reporter Assay Substrate Kit - Firefly ab228530 from Abcam

Product Specs
ItemLuciferase Reporter Assay Substrate Kit - Firefly
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228530
Quantity1 Unit, 10 Unit, 100 Unit
Sample TypeCell culture extracts, Adherent cells, Cell culture media
ApplicationsEnzyme activity assay
Research AreaImmunology & Infectious Disease

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) HIF‐1α‐induced expression of the m6A reader YTHDF1 inhibits the ferroptosis of nucleus pulposus cells by promoting SLC7A11 translationAging CellSeptember 1, 2024Xiao Lu, Dachuan Li, Zhidi Lin, Tian Gao, Zhaoyang Gong, Yuxuan Zhang, Hongli Wang, Xinlei Xia, Feizhou Lu, Jian Song, et al.plasmids into NPCs, and luciferase activity was detected using a dual‐luciferase reporter assay kit (ab228530, Abcam). 2.11 Chromatin immunoprecipitation (ChIP)‐PCR Experiments were performed according to the

(2) Small Extracellular Vesicles Released from miR-211-5p-Overexpressed Bone Marrow Mesenchymal Stem Cells Ameliorate Spinal Cord Injuries in RatseNeuroFebruary 1, 2024Wang X, Ye L, Zhang K, Gao L, Xiao J, Zhang Y.detection of EVs. Dual-luciferase reporter assay Luciferase Reporter Assay Substrate Kit - Firefly (ab228530; Abcam) was used for the dual-luciferase reporter assay in this study following the operating instructions. Osteogenic

(3) LINC00857 regulated by ZNF460 enhances the expression of CLDN12 by sponging miR-150-5p and recruiting SRSF1 for alternative splicing to promote epithelial-mesenchymal transformation of pancreatic adenocarcinoma cellsRNA BiologyDecember 31, 2022Yong Zhang, Yuan Fang, Lijie Ma, Jing Xu, Chentao Lv, Li Deng, Guanghui Zhuco-transfected with pcDNA3.1 or pcDNA3.1-ZNF460. After that, Luciferase Reporter Assay Substrate Kit (ab228530, Abcam, Shanghai, China) was utilized and the luciferase activities were detected via GloMax20/20 Luminometer

(4) miR-29b Regulates Lung Cancer Progression by Downregulating FEM1B and Inhibiting the FOX01/AKT PathwayComputational and Mathematical Methods in MedicineAugust 14, 2022Huanrong Zhang, Rong Wang, Qiuhua Dengab81283)), AKT (Abcam; ab8805), VEGF (Abcam; ab32152), GAPDH (Abcam; ab8245), and secondary antibody (Abcam; ab228530). 2.8. Prediction of the miRNA Target Interaction At least two prediction databases, such as miRTarBase

(5) Role of TRIM22 in ulcerative colitis and its underlying mechanismsMolecular medicine reportsAugust 1, 2022Ye B, Lu Z.activity was detected according to the instructions of a dual-luciferase reporter gene assay kit (cat. no. ab228530; Abcam). In brief, the pGL3-KLF2 or pGL3 vector (Promega Corporation) was co-transfected into HT-29 cells

(6) Cardioprotective Effect of Paeonol on Chronic Heart Failure Induced by Doxorubicin via Regulating the miR-21-5p/S-Phase Kinase-Associated Protein 2 AxisFrontiers in Cardiovascular MedicineJuly 5, 2022Cong Chen, Shuhong Liu, Gaozhen Cao, Yang Hu, Run Wang, Min Wu, Mingya Liu, Kai Hang YiuStates), pGL3-basic vectors (VT1554, YouBio, Hunan, China), commercial dual-luciferase reporter assay kit (ab228530, Abcam), TRIzol (R0016, Beyotime), isopropanol (H822173, Macklin, Shanghai, China), miRNA isolation

(7) Comprehensive analysis of the correlations of S100B with hypoxia response and immune infiltration in hepatocellular carcinomaPeerJMarch 29, 2022Jia Yan, Ya jun Huang, Qing yu Huang, Peng Xia Liu, Chang Shan WangSubsequently, the cells were washed with PBS and treated using the Luciferase Reporter Assays Substrate Kit (ab228530; Abcam, Cambridge, MA, USA). Then, the Dual Glo Luciferase Assay System (Promega, Madison, WI, USA)

(8) Long noncoding RNA endogenous bornavirus-like nucleoprotein acts as an oncogene by regulating microRNA-655-3p expression in T-cell acute lymphoblastic leukemiaBioengineeredMarch 1, 2022Jinhua Yang, Yingying Yangcontrol for 48 h. Luciferase activity was measured using a luciferase reporter assay kit (Abcam, cat no. ab228530). RNA pull-down assay The interaction between lncRNA EBLN3P and miR-655-3p was verified using an RNA pull-down

(9) MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin PathwayInternational journal of stem cellsNovember 30, 2021Li Q, Huang L.or inhibitor by Lipofectamine 2000 reagent for 48 h. After being administrated with the working kit (ab228530, Abcam, USA), the cells were added into the SpectraMax reader (Molecular Devices, Shanghai, China) for

(10) Exosomal miRNA-205 promotes breast cancer chemoresistance and tumorigenesis through E2F1AgingJuly 22, 2021Zhao Y, Jin LJ, Zhang XY.luciferase activities were quantified using the Luciferase Reporter Assay Substrate Kit (Catalog #: ab228530; Abcam, Cambridge, MA, USA). Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from exosomes,

(11) Circular RNA CHST15 Sponges miR-155-5p and miR-194-5p to Promote the Immune Escape of Lung Cancer Cells Mediated by PD-L1Frontiers in OncologyMarch 11, 2021Jianru Yang, Yang Jia, Bing Wang, Shengrong Yang, Kun Du, Yujie Luo, Yunhe Li, Bing Zhumimic, miR-194-5p mimic, or MC for 48 h, and then performed with dual-luciferase reporter experiment (ab228530, Abcam, CA, USA). The absorbance of the 48-well plates was detected by SpectraMax reader (Molecular

(12) LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axisCancer cell internationalJanuary 6, 2021Zhao P, Cheng J, Li B, Nie D, Wang H, Li C, Gui S, Zhang Y.mimic. After transfection, the cells were treated with a Luciferase Reporter Assays Substrate Kit (ab228530, Abcam, CA, USA) for the detection of luciferase activity under a SpectraMax reader (Molecular Devices,

Be the First to Write a Review!

Luciferase Reporter Assay Substrate Kit - Firefly from Abcam

Abcam

Citations (12)

Reviews

Luciferase Reporter Assay Substrate Kit - Firefly from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map