Trypsin-EDTA solution is a mixture commonly used for cell and tissue dissociation. Trypsin is a digestive protease, usually of porcine origin, that is used for its strong proteolytic action. As an endopeptidase, it cleaves proteins internally at lysine and arginine residues. EDTA is a chelator that sequesters metal ions such as calcium and magnesium. EDTA enhances the cleavage ability of trypsin to help weaken cell adhesion in cell suspensions. In some formulations, phenol red is added as a pH indicator. Among its applications, Trypsin-EDTA can be used to generate single-cell lines for stem cell research.
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- Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide ...
- 100 mL
- Cell Culture
- Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide ...
- 100 mL
- Cell Culture
- Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide ...
- 100 mL
- Cell Culture
- Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide ...
- 100 mL
- Cell Culture
- Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide ...
- 100 mL
- Supplements & Reagents
- The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin ...
- 100 mL
- Cell Assay
Select up to 5 products from above to compare or request more information.
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