DMEM Media

DMEM, or Dulbecco’s Modified Eagle Medium is a widely used modification to Harry Eagle’s Minimal Essential Media (MEM). It contains four times the amount of vitamins and amino acids, increased glucose, and additional supplements such as iron. The original recipe includes 1000 mg/L of glucose, but different varieties now exist containing up to 4500 mg/L. Depending on the application, amounts of other components may also differ, such as sodium pyruvate, sodium bicarbonate, L-glutamine, HEPES, and phenol red. First developed to grow mouse embryonic cells, DMEM is now extensively used in culturing a wide variety of mammalian cell types and cell lines.