Alkaline Phosphatase Substrates

Alkaline phosphatase (AP) substrates are used to facilitate the detection of protein targets in applications such as immunohistochemistry, western blotting, and ELISA. Some of the most widely used compounds include BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate along with nitro blue tetrazolium), pNPP (para-Nitrophenylphosphate), and Fast Red. These substrates produce a detectable color change when added to alkaline phosphatase labels, typically conjugated to antibodies. When choosing a proper AP substrate, consider the colors present in the final staining to ensure easy differentiation.

Alkaline Phosphatase Substrates

Your search returned 10 Alkaline Phosphatase Substrates across 1 supplier.
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4-Aminophenyl phosphate monosodium salt, Alkaline phosphatase substrate
  • Quantity:
    10 MG, 50 MG
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Alkaline Phosphatase chromogen (BCIP/TNBT)
  • Quantity:
    100 ml
  • Applications:
    Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(1)
BCIP disodium salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 MG, 500 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
BCIP toluidine salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 MG, 500 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Fast red TR 1,5-naphthalenedisulfonate salt, Phosphatase detection reagent
  • Quantity:
    1 G, 5 G
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(2)
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
  • Quantity:
    100 ml
  • Applications:
    Dot Blot, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(4)
Nitrotetrazolium blue chloride (NBT), Chromogenic substrate
  • Quantity:
    1 G, 5 G, 250 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(4)
Nuclear fast red, nucleic acid stain
  • Quantity:
    5 g
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(2)
pNPP-Na hexahydrate, Alkaline phosphatase substrate
  • Quantity:
    5 G, 25 G
  • Applications:
    ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Fast Red Substrate Kit
  • Quantity:
    60 ML, 125 ML
  • Applications:
    Staining, Blotting, Immunohistochemistry, Immunohistochemistry - fixed, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(9)
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