Alkaline Phosphatase Substrates

Alkaline Phosphatase Substrates Alkaline phosphatase (AP) substrates are used to facilitate the detection of protein targets in applications such as immunohistochemistry, western blotting, and ELISA. Some of the most widely used compounds include BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate along with nitro blue tetrazolium), pNPP (para-Nitrophenylphosphate), and Fast Red. These substrates produce a detectable color change when added to alkaline phosphatase labels, typically conjugated to antibodies. When choosing a proper AP substrate, consider the colors present in the final staining to ensure easy differentiation.

Alkaline Phosphatase Substrates

Your search returned 6 Alkaline Phosphatase Substrates across 1 supplier.
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4-Aminophenyl phosphate monosodium salt, Alkaline phosphatase substrate
  • Quantity:
    10 mg, 50 mg
  • Detection Method:
    Colorimetric
  • Format:
    Solid
BCIP disodium salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 mg, 500 mg
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(2)
BCIP toluidine salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 mg, 500 mg
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Nitrotetrazolium blue chloride (NBT), Chromogenic substrate
  • Quantity:
    1 g, 5 g, 250 mg
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(9)
pNPP-Na hexahydrate, Alkaline phosphatase substrate
  • Quantity:
    5 g, 25 g
  • Applications:
    ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(1)
Nuclear fast red, nucleic acid stain
  • Quantity:
    5 g
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(2)
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