Alkaline Phosphatase Substrates

Alkaline Phosphatase Substrates Alkaline phosphatase (AP) substrates are used to facilitate the detection of protein targets in applications such as immunohistochemistry, western blotting, and ELISA. Some of the most widely used compounds include BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate along with nitro blue tetrazolium), pNPP (para-Nitrophenylphosphate), and Fast Red. These substrates produce a detectable color change when added to alkaline phosphatase labels, typically conjugated to antibodies. When choosing a proper AP substrate, consider the colors present in the final staining to ensure easy differentiation.

Alkaline Phosphatase Substrates

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pNpp substrate, (100 ml)

pNpp substrate, (100 ml)

Enzo Life Sciences, Inc.

  • Quantity:
    100 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
HIGHDEF&#174; Green AP Chromogen/Substrate

HIGHDEF® Green AP Chromogen/Substrate

Enzo Life Sciences, Inc.

  • Quantity:
    30 ml
  • Applications:
    In Situ Hybridization, Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
HIGHDEF&#174; blue IHC chromogen (AP)

HIGHDEF® blue IHC chromogen (AP)

Enzo Life Sciences, Inc.

  • Quantity:
    30 ml
  • Applications:
    In Situ Hybridization, Staining, Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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HIGHDEF&#174; red IHC chromogen (AP, plus)

HIGHDEF® red IHC chromogen (AP, plus)

Enzo Life Sciences, Inc.

  • Quantity:
    30 ml
  • Applications:
    In Situ Hybridization, Staining, Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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