PureLink RNA Mini Kit 12183025 from Thermo Fisher Scientific

Product Specs
ItemPureLink RNA Mini Kit
CompanyThermo Fisher Scientific
Price $1,050.00 Inquire
Catalog Number12183025
Quantity250 preps
ApplicationsRT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
SourceBacterial, Plant, Fungal, Cell/Tissue, Blood, Plasma/Serum
Nucleic Acid TypeTotal RNA
Sample TypeCell and tissue samples
Assay Time20 min. (After sample homogenization)
YieldUp to 350 µg
FormatColumn-based

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.microcentrifuge tube. An equal volume of 70% absolute ethanol was added. PureLink RNA Mini Kit (Invitrogen, 12183025) was used to purify the extracted total RNA following the manufacturer’s protocol. Total RNA concentration

(2) Prolonged KRAS-MAPK Inhibition Induces Interferon Signaling that Promotes Cell State Transition and Confers Therapeutic VulnerabilitiesCancer ResApril 20, 2026Ashenafi Bulle, Yali Chen, Huaping Li, Timothy Hung-Po Hung-Po Chen, Iftikhar Ali Khawar, Lin Li, Yu Wang, Peng Liu, Vikas Kumar Somani, Richard Kurupi, et al.Quantitative (real-time) PCR Total RNA was isolated using PureLinkTM RNA Mini Kit (Thermo Fisher Scientific Cat. #12183025), cDNA was generated using High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific, 4368814)

(3) Caspase-6 Controls Lipid and Energy Metabolism in Diet-Induced ObesityAdv Sci (Weinh)April 13, 2026Abhishek Gupta, Wenjing You, Linmeng Han, Jianfei Ji, Meixia Pan, Xianlin Han, Xiaoli Sun, Peng Zhaocultured cells, lysis was performed using the lysis buffer from the PureLink RNA Mini Kit (Life Technologies #12183025), and RNA was isolated following the manufacturer's instructions. For RNA isolation from mouse tissues,

(4) The plant hormone, 6-benzylaminopurine, ameliorates obesity in male and female mice while on a high-fat dietMol MetabApril 11, 2026Calvin V. Lieu, Cindy X. Zhang, Neruja Loganathan, Leon French, Andre Krunic, Sarah B. Cash, Jin Shi, Juliette Lee, Kacey J. Prentice, Carolyn L. Cummins, et al.culture experiments, total RNA was isolated using the Purelink RNA isolation mini kit (Invitrogen, 12183025) according to the manufacturer’s instructions with the on-column DNase step (Invitrogen, 12185010) to

(5) Expression profiling of inhibitory immune checkpoints in colorectal cancer stem cells and their association with tumor immunity and immunotherapy biomarkersFront ImmunolApril 8, 2026Ola J. Hussein, Muhammad Ammar Zahid, Hanan H. Abunada, Abdelali Agouni, Cristina Maccalli, Hesham M. Korashypolymerase chain reaction Total RNA was extracted from cells using PureLink™ RNA Mini Kit (Invitrogen, cat. 12183025) according to the manufacturer’s instructions. The purity and concentration of RNA were determined by

(6) Low‐dose lithium supplementation promotes musculoskeletal and metabolic health in ovariectomized female miceThe Journal of PhysiologyApril 1, 2026Bianca M. Marcella, Jessica L. Braun, Amélie A.T. Marais, Briana L. Hockey, Mia S. Geromella, Ryan W. Baranowski, Rene Vandenboom, Rebecca E.K. MacPherson, Val A. FajardoThermoFisher Scientific, MI, USA), and RNA was extracted using the Invitrogen PureLink RNA Mini Kit (12183025, ThermoFisher Scientific). Total RNA sequencing with purified mRNA was performed by Novogene (Novogene

(7) Disease context dictates the cellular targets of IL-17 in inflammatory skin diseasebioRxivMarch 27, 2026Kellen J. Cavagnero, Fengwu Li, Haley Jo, Carlos Aguilera, Jennifer Fox, Joseph Kirma, Rachael Bogel, J. Michelle Kahlenberg, Lam C. Tsoi, Johann E. Gudjonsson, et al.(AM7020; Thermo Fisher Scientific). Samples were lysed and RNA was isolated using PureLink Isolation kit (12183025; Thermo Fisher Scientific) and made into cDNA using Verso cDNA synthesis kit (AB1453/B; Thermo Fisher Scientific

(8) Acid Ceramidase Inhibition Disrupts Ceramide Homeostasis and Induces Mitochondrial Apoptosis in IDH1-Mutant OligodendrogliomaResearch SquareMarch 25, 2026Mioara Larion, Helena Muley, Faris Zaibaq, Meili Zhang, Dionne Davis, Michael Kruhlak, Samarth Mathur, Adrian Lita, Hua Song, Wei Zhang, et al.and stored at −80 °C until RNA extraction. Total RNA was purified using the PureLink™ RNA Mini Kit (12183025, Invitrogen), including an on-column DNase digestion step using the PureLink™ DNase Set (12185010, Invitrogen). RNA Sequencing

(9) Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cellsCell ReportsMarch 24, 2026Kevin C. Corn, Shannon E. Martello, Vinay K. Menon, Lucy S. Britto, Kara M. Simmons, Youssef K. Mohamed, Yoanna I. Ivanova, Abtin A. Ghelmansaraei, Sara A. Weidenbach, Tian Zhu, et al.lysed in Trizol (Invitrogen 155960), and RNA was isolated using the PureLink RNA Mini Kit (Invitrogen 12183025) according to the manufacturer’s protocol. For RT-qPCR, 500ng of total RNA was reverse transcribed using

(10) Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programsiScienceMarch 20, 2026Maya R. MacLean, Bianca Laura Bernardoni, Wasundara Fernando, Giovanni Petrarolo, Ilaria D’Agostino, Cheryl A. Dean, Jaganathan Venkatesh, Christopher S. Hughes, Kerry B. Goralski, Geetha Subramanian, et al.Kit,BioRad,Cat#1610183 Aldefluor Assay Kit,Stemcell,Cat#01700 Purelink RNA Mini Kit,Invitrogen,Cat#12183025 iScript cDNA Synthesis Kit,BioRad,Cat#1708841 Creatinine (serum) Colorimetric Assay Kit,Cayman Chemical,Cat

(11) Inflammatory Mediators Both Directly and Indirectly Promote Microglial ProliferationGliaMarch 19, 2026Brady P. Hammond, Eugene Hahn, Kelly V. Lee, Bradley J. Kerr, Jason R. Plemelng/mL. After 72 h, we extracted RNA following the protocol of the PureLink RNA Mini Kit (Invitrogen 12183025). Briefly, we replaced treatment media with warm PBS followed by a solution of RLT buffer and β‐mercaptoethanol

(12) Non-invasive host transcriptome and HPV oncogene expression map the molecular landscape of HPV-driven cervical lesionsPLoS OneMarch 19, 2026Mohamad Ammar Ayass, Naila Zaman, Natalya Griko, Victor Pashkov, Kevin Zhu, Ghulam Abbas, Melesse Ghelan, Ramya Ramankutty Nair, Tutku Okyay, Jin Zhang, et al.RNA was subsequently isolated using the PureLinkTM RNA Mini Kit (Thermo Fisher Scientific, Cat. No. 12183025), following the manufacturer’s “TRIzol Plus Total Transcriptome Isolation” protocol. RNA yield and purity

(13) VHL synthetic lethality screens uncover CBF-β as a negative regulator of STINGNature CommunicationsMarch 12, 2026James A. C. Bertlin, Tekle Pauzaite, Qian Liang, Niek Wit, James C. Williamson, Jia Jhing Sia, Nicholas J. Matheson, Brian M. Ortmann, Thomas J. Mitchell, Anneliese O. Speak, et al.(ACTB). RNA sequencing Total RNA was extracted using the PureLink RNA Mini Kit (ThermoFisher Cat#12183025), including on-column DNA digestion with PureLink DNase. Library preparation and sequencing were undertaken

(14) A druggable redox switch on SHP1 controls macrophage inflammationNature Chemical BiologyMarch 12, 2026Mei Ying Ng, Meredith N. Nix, Guangyan Du, Ivan Davidek, Nils Burger, Sanghee Shin, Sean Toenjes, Haruna Takeda, Megan Cheah Xin Cheah Xin Yan, Bingsen Zhang, et al.extracted using TRIzol (Invitrogen, #15596026), purified with PureLink™ RNA Mini Kit (Invitrogen, #12183025) according to manufacturer’s protocol, and quantified using a Nanodrop™ One/OneC Microvolume UV-Vis

(15) Lafora disease gene therapy: EPM2A but not EPM2B overexpression results in Lafora body formationNeurotherapeuticsMarch 12, 2026Esther O. Alao, Mehrnaz Sheibani, Jun Wu, Ummay Marriam, Doretha Evans, Sahba Kasiri, Mayank Verma, Silvia Nitschke, Felix Nitschke, Steven J. Gray, et al.(Invitrogen, Cat. #520809) followed by purification with the PureLink RNA Mini Kit (Invitrogen, Cat. #12183025), according to the manufacturer's protocol. Complementary DNA (cDNA) was synthesized from 1 μg of total

(16) Sex-specific lymphatic responses to estrogen shape atherosclerosis in high-risk miceFront Cardiovasc MedMarch 11, 2026Mona Mesples, Élizabeth Lacroix, Nolwenn Tessier, Maya Farhat, Andreea Milasan, Sara Babran, Cristina Fernandez, Tally Latendresse, Justin Benoit, Valérie Long, et al.ensure sufficient RNA yield. RNA was extracted according to the manufacturer's protocol (Invitrogen, 12183025) and stored at −80 °C. Reverse transcription was carried out using the High-Capacity cDNA Reverse Transcription

(17) Longitudinal plasma interleukin-6 and post-stroke cognitive outcomes: The Stroke-IMPaCT studyAlzheimers DementMarch 10, 2026Natasha S. Carmichael, Harry R. Deijnen, Siew Yan Wong, Thomas O. Williams, Evangelos Kontopantelis, Luke Cowie, Eileen Jones, Lauren Drag, Marion S. Buckwalter, John R. Grainger, et al.analyses Total RNA was extracted from PBMCs using the PureLink RNA Mini Kit (Cat. Nos. 12183018A, 12183020, 12183025; ThermoFisher Scientific), following the manufacturer's protocol. An on‐column DNase digestion step

(18) Dual phagocytosis-checkpoint blockade revitalizes immune surveillance in mouse models of glioblastomaNature CommunicationsMarch 9, 2026JongHoon Ha, Yifan Wang, Yifan Ma, Annette Wu, Shiyan Dong, Seong Dong Jeong, Jared L. Edwards, Mengyu Chang, Yen-Tzu Chang, Xiaotian Wang, et al.described for western blot. RNA samples were extracted using a PureLink RNA Mini kit (ThermoFisher, #12183025) according to the manufacturer’s protocol. Isolated RNA was reverse transcribed into complementary DNA

(19) Liver exerkine reverses aging- and Alzheimer’s-related memory loss via vasculatureCellMarch 5, 2026Gregor Bieri, Karishma J.B. Pratt, Yasuhiro Fuseya, Turan Aghayev, Juliana Sucharov, Alana M. Horowitz, Amber R. Philp, Karla Fonseca-Valencia, Rebecca Chu, Mason Phan, et al.(Sigma-Aldrich, Cat#T9424) in combination with the PureLink RNA Mini Kit (Thermo Fisher Scientific Cat# 12183025) or Direct-zol™ RNA Purification Miniprep Plus Kit (Zymo Research Cat# R2072) following the manufacturer’s

(20) Therapeutic Targeting of Microglial Hexokinase-2 Recalibrates Inflammasome Activation and Improves Functional Recovery After Traumatic Brain InjurybioRxivMarch 3, 2026Claudia Mera-Reina, Juan F. Codocedo, Paul B. Fallen, Jack Scott, Cristian A. Lasagna-Reeves, Gary E. Landrethquantitative real-time PCR RNA was extracted from homogenized tissue using the PureLink RNA Mini Kit (Invitrogen, Ref 12183025) following the manufacturer’s instructions. The RNA was then reverse-transcribed into cDNA with the

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1/11/2024Easy and Fast Way to Isolate RNA Using PureLink™ RNA Mini Kit From ThermoFisher Kateryna MurlanovaWe use the PureLink™ RNA Mini Kit from ThermoFisher to isolate RNA from primary mouse astrocytes for subsequent RNA-seq or qRT-PCR.

PureLink RNA Mini Kit from Thermo Fisher Scientific

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PureLink RNA Mini Kit from Thermo Fisher Scientific

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