Fig 2: (A) Caspase 1 gene expression on HC69.5. HIV (GFP+) cells was assessed by RT PCR. H69.5 cells treated with/without 100 µg/ml Poly IC, 1 µM concentration of CBD or/and ? (9)-THC. After 6 h and 24 h of treatments, RNAs were extracted, and reverse transcribed followed by real time PCR for Caspase 1 gene (Hs00354836_m1; Thermofisher Scientific). GAPDH (Hs99999905_m1) was used as a housekeeping gene. Data represent the means ± standard error of three independent experiments. Graph represents the transcript accumulation index respect to negative control C20. Data were analyzed using GraphPad Prism software. The normality distribution was evaluated by Kolmogorov–Smirnov test, while statistical significance was calculated by ANOVA and Tukey Multiple Comparison Test as a post hoc test. Differences were considered significant at p = 0.05. (B) Caspase 1 Activation. HIV infection in HC69.5. HIV (GFP+) with and without 100 µg/ml poly IC and cells were treated with 1 µM of ?(9)-THC or CBD. After 24 h. of treatment, Caspase 1 activation was analyzed using Caspase-Glo® 1 Inflammasome Assay kit (Promega; cat: G9951). (Ct = Control). GraphPad Prism 5 software was used to graph and conduct statistical analysis. Normality distribution was checked by the Kolmogorov–Smirnov test. Then, statistical significances (p = 0.05) were calculated by ANOVA followed by Dunn ‘s as a post-hoc test.