MTS Assay Kit (Cell Proliferation) (Colorimetric) ab197010 from Abcam

Product Specs
ItemMTS Assay Kit (Cell Proliferation) (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab197010
Sample TypeAdherent cells, Suspension cells
Assay Time4h 0m
ApplicationsCellular Assay
TypeQuantitative
Detection TargetCell viability/proliferation
Quantity250 TEST, 500 TEST, 2500 TEST, 5000 TEST, 10000 TEST

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Upregulated LAMA3 modulates proliferation, adhesion, migration and epithelial‑to‑mesenchymal transition of cholangiocarcinoma cellsSci RepDecember 18, 2023Kittiya Islam, Brinda Balasubramanian, Simran Venkatraman, Parichut Thummarati, Janpen Tunganuntarat, Nut Phueakphud, Phongthon Kanjanasirirat, Tanawadee Khumpanied, Pornparn Kongpracha, Yingpinyapat Kittirat, et al.reagent (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium) (ab197010; Abcam) to each well and further incubation for four hours according to the manufacturer’s instructions.

(2) Anti-tumor activity of all-trans retinoic acid in gastric-cancer: gene-networks and molecular mechanismsJournal of Experimental & Clinical Cancer ResearchNovember 11, 2023Luca Guarrera, Mami Kurosaki, Silvio-Ken Garattini, Maurizio Gianni’, Gianpiero Fasola, Luca Rossit, Michele Prisciandaro, Maria Di Bartolomeo, Marco Bolis, Paola Rizzo, et al.(BD Life Sciences). Cell growth studies were performed with the colorimetric MTS-assay kit (Abcam, ab197010), the Sulforhodamine assay [16] or an automatic counting of viable cells with the use of the Vi-cell

(3) Effect of Oleoylethanolamide-Based Dietary Supplement on Systemic Inflammation in the Development of Alimentary-Induced Obesity in MiceNutrientsOctober 12, 2023Darya Ivashkevich, Arina Ponomarenko, Igor Manzhulo, Ruslan Sultanov, Inessa Dyuizenovernight. Cells cultured in standard medium were used as a negative control. The next day, MTS reagent (ab197010, Abcam, Cambridge, MA, USA) was added to each well and incubated for 2 h at 37 °C. The optical density

(4) L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5MoleculesOctober 10, 2023Junhe Shi, Trevor R. Leonardo, Chen Han, Hiba I. Bangash, Dandan Chen, Harsh M. Trivedi, Lin Chenproliferation was examined using an MTS Cell Proliferation Assay Kit (Abcam, Waltham, MA USA, Catalog # ab197010), according to the manufacturer’s instructions, at 24, 72, and 96 h post-treatment for low-glucose (6

(5) Paeonol accelerates skin wound healing by regulating macrophage polarization and inflammation in diabetic ratsThe Korean Journal of Physiology & PharmacologySeptember 1, 2023Zuyang Zhang, Tianhua Chen, Wei Liu, Jiepeng Xiong, Liangdong Jiang, Mingjiang Liuwas later examined by a chemiluminescence imaging system (Bio-Rad). Cell viability test A MTS kit (ab197010, Abcam) was used to detect the effect of paeonol on macrophage viability. RAW264.7 macrophages were

(6) Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsitySci AdvAugust 18, 2023Yuying Tan, Haonan Lin, Ji-Xin Chengmetal-oxide semiconductor camera (Thorlabs, CS165CU). Cell viability assay The MTS assay (Abcam, #ab197010) was used to measure cell viability. After overnight seeding of cells in 96-well plates at a density

(7) BRD4 Inhibition as a Strategy to Prolong the Response to Standard of Care in Estrogen Receptor-Positive Breast CancerCancers (Basel)August 11, 2023Ahmed M. Elshazly, Melanie M. Sinanian, Victoria Neely, Eesha Chakraborty, Muruj A. Alshehri, Michael K. McGrath, Hisashi Harada, Patricia V. Schoenlein, David A. Gewirtztreated under the indicated conditions. The assay was performed according to the manufacturer’s protocol (AB197010, Abcam) and absorbance, which correlates to the number of viable cell per well, was recorded at 490

(8) Screening of lactic acid bacteria with anti-adipogenic effect and potential probiotic properties from grainsScientific ReportsJuly 7, 2023Min Ju Seo, Sung-Min Won, Min Ju Kwon, Ji Hyeon Song, Eun Bee Lee, Jun Hyeong Cho, Kye Won Park, Jung-Hoon YoonCell viability assay Cell viability was determined using methyl thiazolyl tetrazolium salt (MTT) colorimetric assays (ab197010, Abcam). C3H10T1/2 cells were seeded at 1.5 × 104 cells per well in 96-well plates, then treated with

(9) Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replicationThe Journal of cell biologyJuly 3, 2023Williams JM, Chen YJ, Cho WJ, Tai AW, Tsai B.transfected with the indicated siRNAs for either 48 h or 96 h. The cells were treated with the MTS reagent (ab197010; MTS Assay Kit, Abcam) according to the manufacturer's protocol and incubated at 37°C under 5% CO2 for

(10) mLST8 is essential for coronavirus replication and regulates its replication through the mTORC1 pathwaymBioJune 28, 2023Yanan Fu, Zhen Fu, Zhelin Su, Lisha Li, Yilin Yang, Yubei Tan, Yixin Xiang, Yuejun Shi, Shengsong Xie, Limeng Sun, et al.fluorescence assays were performed. The MTS assay was performed using the MTS assay kit (Abcam, no. ab197010). Cells were seeded into 96-well plates and incubated in a 37°C incubator for 12 hours. The culture

(11) Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patientsMolecular MedicineMay 24, 2023Luisa Tengler, Julia Schütz, Moritz Tiedtke, Jadwiga Jablonska, Marie-Nicole Theodoraki, Katja Nitschke, Christel Weiß, Elena Seiz, Annette Affolter, Frederic Jungbauer, et al.used. MTS cell proliferation assay was performed according to the manufacturer’s instructions (Abcam, #ab197010). Briefly, the MTS reagent was added at a dilution of 1:10 and incubated for 3 h. Absorbance was measured

(12) Evaluation of immunogenicity of gene-deleted and subunit vaccines constructed against the emerging pseudorabies virus variantsVirology journalMay 23, 2023Zhang HL, Zhang RH, Liu G, Li GM, Wang FX, Wen YJ, Shan H.inhibiting PK15 cell infection. Lymphocyte proliferation was analyzed using an MTS assay kit (Abcam, ab197010, Shanghai, China). Peripheral blood mononuclear cells (PBMC) were prepared using peripheral blood lymphocyte

(13) Swarming self-adhesive microgels enabled aneurysm on-demand embolization in physiological blood flowScience AdvancesMay 12, 2023Dongdong Jin, Qinglong Wang, Kai Fung Chan, Neng Xia, Haojin Yang, Qianqian Wang, Simon Chun Ho Yu, Li Zhangserum, and phosphate-buffered saline (PBS) were from Gibco–Thermo Fisher Scientific, and MTS assay (ab197010) was from Abcam. All the chemicals were used without further purification. Preparation of self-healing

(14) Metabolic rewiring in keratinocytes by miR‐31‐5p identifies therapeutic intervention for psoriasisEMBO Molecular MedicineApril 11, 2023Mao‐Jie Wang, Huan‐Jie Huang, Yong‐Yue Xu, Harmjan Vos, Can Gulersonmez, Edwin Stigter, Johan Gerritsen, Marc Pages Gallego, Robert van Es, Li Li, et al.were plated in 96‐well microtiter plate and received different treatments before MTS assay (Abcam, ab197010). For the MTS assay, 20 μl of MTS reagent was directly added into the culture medium and incubated at

(15) Next generation oncolytic viruses expressing PADI1 and TIMP2 exhibit anti-tumor activity against melanoma in nude and humanized mouse modelsMolecular Therapy - OncolyticsMarch 16, 2023Lukasz Kuryk, Anne-Sophie W. Mølleron the same day. Viability was assessed 72 h after treatment with the MTS assay kit (catalog number ab197010; Abcam), according to the manufacturer’s instructions. ELISA analyses A549 infected cells were harvested

(16) CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neuronal cell deathScientific reportsMarch 9, 2023Pavlou S, Foskolou S, Patikas N, Field SF, Papachristou EK, Santos C, Edwards AR, Kishore K, Ansari R, Rajan SS, et al.was used for cortical or dopaminergic neurons, respectively. Viability was measured by MTS assay (#ab197010, Abcam) according to manufacturer’s instructions. CRISPR-Cas9 screen A druggable genome sgRNA library

(17) Demonstration of doxorubicin's cardiotoxicity and screening using a 3D bioprinted spheroidal droplet-based system†‡RSC AdvancesMarch 8, 2023Raven El Khoury, Salma P. Ramirez, Carla D. Loyola, Binata Joddar(Thermo Fisher Scientific, USA, Cat. No. 174140250) were used as ROS scavenging agents. MTS Assay Kit (Colorimetric) (ab197010) was procured from Abcam, MA, USA and the CellTiter 96® Aqueous One Solution Cell Proliferation Assay

(18) Pervasive environmental chemicals impair oligodendrocyte developmentbioRxiv : the preprint server for biologyFebruary 12, 2023Cohn EF, Clayton BLL, Madhavan M, Yacoub S, Federov Y, Paul-Friedman K, Shafer TJ, Tesar PJ.3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol. Absorbance at 490 nm was measured 4 hours after the addition

(19) Genome-wide epigenetic and mRNA-expression profiling followed by CRISPR/Cas9-mediated gene-disruptions corroborate the MIR141/MIR200C-ZEB1/ZEB2-FGFR1 axis in acquired EMT-associated EGFR TKI-resistance in NSCLC cellsTranslational Lung Cancer ResearchJanuary 31, 2023Johan Vad-Nielsen, Nicklas Heine Staunstrup, Magnus Lindkvist Kjeldsen, Nina Dybdal, Guillaume Flandin, Claudia De Stradis, Tina Fuglsang Daugaard, Trine Vilsbøll-Larsen, Christoffer Trier Maansson, Thomas Koed Doktor, et al.erlotinib dissolved in DMSO was measured with a colorimetric MTS Cell Proliferation Assay Kit (Abcam, Cat#ab197010) and a Synergy HTX Multi-Mode Reader (Agilent). 5000 cells/well were seeded in 96-Well Plates (Thermo

(20) Bioinspired Oxidation-Resistant Catechol-like Sliding Ring Polyrotaxane HydrogelsGelsJanuary 19, 2023M. Isabel Rial-Hermida, Dora C. S. Costa, Lan Jiang, João M. M. Rodrigues, Kohzo Ito, João F. ManokDa were supplied from Spectrum Laboratories (Waterford, Ireland). MTS cell proliferation tests (Kit ab197010) were acquired from Abcam (Cambridge, UK). 1H and 13C NMR spectra were recorded using a Bruker Avance-300

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MTS Assay Kit (Cell Proliferation) (Colorimetric) from Abcam

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MTS Assay Kit (Cell Proliferation) (Colorimetric) from Abcam

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