ADCC Reporter Bioassay, Core Kit G7010 from Promega

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ItemADCC Reporter Bioassay, Core Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7010
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(41) Mechanisms and targets of Fcγ-receptor mediated immunity to malaria sporozoitesNature CommunicationsDecember 1, 2021Gaoqian Feng et al.of a selection of serum samples was assessed using the ADCC Reporter Bioassay kit (Promega, Cat. No. G7010) as per the manufacturer’s instructions, with the following modifications. CSP-coated beads were opsonized

(42) Protease-Activation of Fc-Masked Therapeutic Antibodies to Alleviate Off-Tumor CytotoxicityFrontiers in ImmunologyAugust 3, 2021Adrian Elter, Desislava Yanakieva, David Fiebig, Kerstin Hallstein, Stefan Becker, Ulrich Betz, Harald Kolmarsoftware. Antibody-Dependent Cell-Mediated Cytotoxicity For ADCC activity determination, Promega ADCC Reporter Bioassay Kit (G7010) (Promega) was used. Assays were performed according to manufacturer instructions. A serial dilution

(43) Asymptomatic and symptomatic SARS-CoV-2 infections elicit polyfunctional antibodiesCell Reports MedicineMay 18, 2021Jérémy Dufloo et al.assays ,, "NK Cell Isolation Kit, human",Miltenyi Biotec,Cat#130-092-657 ADCC Reporter Bioassay,Promega,Cat#G7010 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit,Invitrogen,Cat#L34957 Bright-Glo Luciferase Assay System,Promega,Cat#E2620 ,, Experimental models

(44) Design of a Trispecific Checkpoint Inhibitor and Natural Killer Cell Engager Based on a 2 + 1 Common Light Chain Antibody ArchitectureFrontiers in ImmunologyMay 10, 2021Jan P. Bogen, Stefania C. Carrara, David Fiebig, Julius Grzeschik, Björn Hock, Harald Kolmardescribed previously (54). The ADCC assay was performed utilizing the Promega ADCC Reporter Bioassay Kit (G7010) following the manufacturer’s instructions. The day before the assay, 12.500 A431 cells were seeded

(45) Expeditious Generation of Biparatopic Common Light Chain Antibodies via Chicken Immunization and Yeast Display ScreeningFrontiers in ImmunologyDecember 23, 2020Jan P. Bogen, Stefania C. Carrara, David Fiebig, Julius Grzeschik, Björn Hock, Harald KolmarCell-Mediated Cytotoxicity ADCC assays were performed using the Promega ADCC Reporter Bioassay Kit (G7010) following the manufacturer’s instructions. A fourfold serial dilution of respective antibodies (5 µg/ml

(46) A universal dual mechanism immunotherapy for the treatment of influenza virus infectionsNature communicationsNovember 5, 2020Liu X, Zhang B, Wang Y, Haymour HS, Zhang F, Xu LC, Srinivasarao M, Low PS.cellular cytotoxicity (ADCC) assay Analysis of ADCC was performed using an ADCC kit (Promega, cat. no. G7010) plus N1-transduced and nontransduced HEK 293 cells. For this purpose, cells were plated in triplicate

(47) Malignant tissues produce divergent antibody glycosylation of relevance for cancer gene therapy effectivenessmAbsAugust 4, 2020Dominik Brücher, Vojtech Franc, Sheena N. Smith, Albert J. R. Heck, Andreas Plückthungrowth-arresting effect of trastuzumab on HER2-expressing cells, a signaling-based assay from Promega (Cat# G7010) was used to determine the intensity of the FcRγIIIa-mediated response. For this purpose, 5,000 SKBR3

(48) Pharmacologic Characterization of ALD403, a Potent Neutralizing Humanized Monoclonal Antibody Against the Calcitonin Gene-Related PeptideJournal of Pharmacology and Experimental TherapeuticsJuly 1, 2020Leon F. Garcia-Martinez et al.(Dorokhov et al., 2016). Reporter bioassays were used according to manufacturer’s instructions (cat. nos. G7010 and G9991; Promega Corporation). Briefly, the day before the assay, SK-N-MC cells were harvested using

(49) A Chimeric Japanese Encephalitis Vaccine Protects against Lethal Yellow Fever Virus Infection without Inducing Neutralizing AntibodiesmBioApril 7, 2020Mishra N, Boudewijns R, Schmid MA, Marques RE, Sharma S, Neyts J, Dallmeier K.performed as prescribed by the manufacturer (ADCC reporter bioassay complete kit; Promega; catalog no. G7010). In brief, target cells were prepared by infecting HEK 293T cells with YFV-17D-mCherry in assay medium.

(50) Functional Immune Response to Influenza H1N1 in Children and Adults after Live Attenuated Influenza Virus Vaccination.Scandinavian Journal of ImmunologyOctober 1, 2019Shahinul Islam, Fan Zhou, Sarah Lartey, Kristin G. I. Mohn, Florian Krammer, Rebecca Jane Cox, Karl Albert BrokstadPrism. Antibody dependent cellular cytotoxicity (ADCC) assay The ADCC Reporter Bioassay (Core Kit G7010/G7018, Promega, USA) was used to quantify pre- and post-vaccination ADCC antibodies [15]. MDCK cells

(51) Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoformsmAbsFebruary 1, 2019Wada R, Matsui M, Kawasaki N.buffer. ADCC reporter bioassay ADCC reporter assay was performed with Promega reporter bioassay kit (Cat # G7010) including the nuclear factor of activated T-cells (NFAT) engineered Jurkat cells expressing FcγRIIIa

(52) Conservation of oncofetal antigens on human embryonic stem cells enables discovery of monoclonal antibodies against cancerScientific ReportsDecember 1, 2018Heng Liang Tan et al.cytotoxicity assay ADCC activity was measured using a reporter bioassay (Promega; ADCC Reporter Bioassay, #G7010). The ADCC bioassay was carried out according to the manufacturer’s protocol. Briefly, cells were seeded

(53) Inhibition of Membrane-Bound BAFF by the Anti-BAFF Antibody BelimumabFrontiers in ImmunologyNovember 20, 2018Christine Kowalczyk-Quintas, Dehlia Chevalley, Laure Willen, Camilla Jandus, Michele Vigolo, Pascal SchneiderFcγRIIIa and NFAT-driven luciferase were provided in the ADCC reporter bioassay core kit (Promega, G7010) and used as recommended by the manufacturer. Biotinylations One milligram of belimumab or 1 mg of

(54) Neutralizing Antibodies Inhibit Chikungunya Virus Budding at the Plasma MembraneCell host & microbeSeptember 12, 2018Jin J, Galaz-Montoya JG, Sherman MB, Sun SY, Goldsmith CS, O'Toole ET, Ackerman L, Carlson LA, Weaver SC, Chiu W, et al.Alexa Fluor 488 protein labeling kit,Thermo Fisher Scientific,Cat. #A10235 ADCC reporter bioassay core kit,Promega,Cat. #G7010 Deposited Data,Deposited Data,Deposited Data Egressed CHIKV virion subtomogramaverage,This paper,EMD-7513 NCLP

(55) Chimeric Hemagglutinin Constructs Induce Broad Protection against Influenza B Virus Challenge in the Mouse ModelJournal of VirologyJune 15, 2017Megan E. Ermler, Ericka Kirkpatrick, Weina Sun, Rong Hai, Fatima Amanat, Veronika Chromikova, Peter Palese, Florian Krammerminimal neutralizing titer. ADCC reporter assay. The Promega ADCC Reporter Bioassay kit (catalog number G7010) was used for this experiment. MDCK cells plated onto an opaque 96-well plate (at 50,000 cells per well)

(56) Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143) in chronic lymphocytic leukemiaJournal of hematology & oncologyMay 19, 2017Kashyap MK, Amaya-Chanaga CI, Kumar D, Simmons B, Huser N, Gu Y, Hallin M, Lindquist K, Yafawi R, Choi MY, et al.analysis of ADCC in B-CLL patient primary cells, the ADCC Reporter Bioassay kit from Promega (Catalog #G7010) was used, per instructions from the manufacturer. The ADCC Reporter Bioassay uses engineered Jurkat cells

(57) Transient Humoral Protection against H5N1 Challenge after Seasonal Influenza Vaccination of HumansPLoS ONEJuly 30, 2014Ramon Roozendaal et al.not shown). White bottom plates were used for a commercially available ADCC reporter assay (PROMEGA, G7010). Serum samples were serially diluted in assay buffer (RPMI, 4% ultra-low IgG FBS, 1% (w/v) penicillin/streptomycin

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ADCC Reporter Bioassay, Core Kit

ADCC Reporter Bioassay, Core Kit from Promega

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ADCC Reporter Bioassay, Core Kit from Promega

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