ADCC Reporter Bioassay, Core Kit G7010 from Promega

Product Specs
ItemADCC Reporter Bioassay, Core Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7010
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Generation and optimization of off-the-shelf immunotherapeutics targeting TCR-Vβ2+ T cell malignancyNature communicationsJanuary 15, 2024Ren J, Liao X, Lewis JM, Chang J, Qu R, Carlson KR, Foss F, Girardi M.A29129) and Jurkat-NFAT-luciferase effector cells was from ADCC Reporter Bioassay, Core Kit (Promega, G7010). They are sub-cultured based on manufacture’s instruction. Animals Eight to twelve weeks NOD.Cg-Prkdcscid

(22) A PD-L1/EGFR bispecific antibody combines immune checkpoint blockade and direct anti-cancer action for an enhanced anti-tumor responseOncoImmunologyDecember 31, 2023Laura Rubio-Pérez, Rodrigo Lázaro-Gorines, Seandean L. Harwood, Marta Compte, Rocío Navarro, Antonio Tapia-Galisteo, Jaume Bonet, Belén Blanco, Simon Lykkemark, Ángel Ramírez-Fernández, et al.Microplate Reader (Life Sciences, Tecan). ADCC reporter bioassay The ADCC reporter Bioassay (Promega, cat# G7010) was used following manufacturer´s instructions. Briefly, 1.2 × 104 CHO and CHOEGFR cells/well were

(23) Advanced glycation end product (AGE) targeting antibody SIWA318H is efficacious in preclinical models for pancreatic cancerScientific reportsOctober 7, 2023Rossi GR, Jensen A, Ng S, Yin Z, Li A, Misra A, Von Hoff DD, Gruber L, Gruber M, Han H.ADCC reporter assay was performed using the ADCC Reporter Bioassay Kit from Promega Corporate (CAt# G7010, Madison, WI, USA) by following the manufacturer recommended procedures. This assay uses engineered

(24) Dual impacts of a glycan shield on the envelope glycoprotein B of HSV-1: evasion from human antibodies in vivo and neurovirulencemBioAugust 31, 2023Fukui A, Maruzuru Y, Ohno S, Nobe M, Iwata S, Takeshima K, Koyanagi N, Kato A, Kitazume S, Yamaguchi Y, et al.induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer, Waltham, MA, USA). The assay was performed according

(25) The 2EF Antibody Targets a Unique N-Terminal Epitope of Trop-2 and Enhances the In Vivo Activity of the Cancer-Selective 2G10 AntibodyCancersJuly 22, 2023Guerra E, Trerotola M, Relli V, Lattanzio R, Ceci M, Boujnah K, Pantalone L, Di Pietro R, Iezzi M, Tinari N, et al.Ab-dependent Cellular Cytotoxicity (ADCC) Assay Jurkat NF-κB/NFAT (ADCC Reporter Bioassay, Core Kit, Cat. No. G7010, Promega, Madison, WI, USA) were utilized as effector/reporter cells. MCF-7 breast cancer cells were

(26) Analytical comparability to evaluate impact of manufacturing changes of ARX788, an Anti-HER2 ADC in late-stage clinical developmentPLOS ONEJuly 10, 2023Wayne Yu, Mysore P. Ramprasad, Manoj Pal, Chris Chen, Shashi Paruchuri, Lillian Skidmore, Nick Knudsen, Molly Allen, Ying Buechleraddition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity)

(27) Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid TumorsClinical Cancer ResearchJune 1, 2023Martina S.J. McDermott, Neil A. O'Brien, Benjamin Hoffstrom, KeWei Gong, Ming Lu, Jun Zhang, Tong Luo, Min Liang, Weiping Jia, Jenny J. Hong, et al.(ADCC) reporter assays were performed according to the manufacturer's protocol (Promega, catalog no. G7010). Briefly, 1×104 cells/well/100 μL target cells were cultured in flat-bottom, optical polymer based

(28) Preclinical Evaluation of ON203, A Novel Bioengineered mAb Targeting Oxidized Macrophage Migration Inhibitory Factor as an Anticancer TherapeuticMolecular Cancer TherapeuticsMay 4, 2023Gregor Rossmueller, Irina Mirkina, Barbara Maurer, Verena Hoeld, Julia Mayer, Michael Thiele, Randolf J. Kerschbaumer, Alexander Schinaglfor the ADCC reporter bioassay core kit with human FcγRIIIA V158-variant (high responder, Promega, # G7010), or F158-variant (low responder, Promega, # G9790) effector cells by using an effector to target cell

(29) A PD-L1/EGFR bispecific antibody combines immune checkpoint blockade and direct anti-cancer action for an enhanced anti-tumor responseOncoimmunologyApril 24, 2023Laura Rubio-Pérez, Rodrigo Lázaro-Gorines, Seandean L. Harwood, Marta Compte, Rocío Navarro, Antonio Tapia-Galisteo, Jaume Bonet, Belén Blanco, Simon Lykkemark, Ángel Ramírez-Fernández, et al.Microplate Reader (Life Sciences, Tecan). ADCC reporter bioassay The ADCC reporter Bioassay (Promega, cat# G7010) was used following manufacturer´s instructions. Briefly, 1.2 × 104 CHO and CHOEGFR cells/well were

(30) Using quantitative single molecule localization microscopy to optimize multivalent HER2-targeting ligandsFrontiers in MedicineApril 17, 2023Devin L. Wakefield, Ottavia Golfetto, Raphael Jorand, Sunetra Biswas, Kassondra Meyer, Kendra N. Avery, Cindy Zer, Eliedonna E. Cacao, Steven J. Tobin, Ian J. Talisman, et al.assays were conducted using a luciferase reporter-based core kit according to manufacturer instructions (G7010, Promega). SK-BR-3 cells were used as the target cell line and seeded at 5,000 cells per well in a 96-well

(31) Preclinical Evaluation of ON203, A Novel Bioengineered mAb Targeting Oxidized Macrophage Migration Inhibitory Factor as an Anticancer TherapeuticMol Cancer TherApril 17, 2023Gregor Rossmueller, Irina Mirkina, Barbara Maurer, Verena Hoeld, Julia Mayer, Michael Thiele, Randolf J. Kerschbaumer, Alexander Schinaglfor the ADCC reporter bioassay core kit with human FcγRIIIA V158-variant (high responder, Promega, # G7010), or F158-variant (low responder, Promega, # G9790) effector cells by using an effector to target cell

(32) Generation of a symmetrical trispecific NK cell engager based on a two-in-one antibodyFrontiers in ImmunologyApril 4, 2023Julia Harwardt, Stefania C. Carrara, Jan P. Bogen, Katrin Schoenfeld, Julius Grzeschik, Björn Hock, Harald Kolmarcytotoxicity (ADCC) reporter assay The ADCC assay was performed using the Promega ADCC Reporter Bioassay Kit (G7010) according to the manufacturer’s protocol. The day before the assay, 10.000 A431 cells were seeded into

(33) Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid TumorsClin Cancer ResMarch 8, 2023Martina S.J. McDermott, Neil A. O'Brien, Benjamin Hoffstrom, KeWei Gong, Ming Lu, Jun Zhang, Tong Luo, Min Liang, Weiping Jia, Jenny J. Hong, et al.(ADCC) reporter assays were performed according to the manufacturer's protocol (Promega, catalog no. G7010). Briefly, 1×104 cells/well/100 μL target cells were cultured in flat-bottom, optical polymer based

(34) Fc-mediated functions of nirsevimab complement direct respiratory syncytial virus neutralization but are not required for optimal prophylactic protectionFrontiers in immunologyJanuary 1, 2023Brady T, Cayatte C, Roe TL, Speer SD, Ji H, Machiesky L, Zhang T, Wilkins D, Tuffy KM, Kelly EJ.(NFAT) luciferase reporter were used as a surrogate for NK cells in the ADCC reporter bioassay (Promega G7010). Jurkat cells were thawed following manufacturer recommendations (37°C water bath for 2–3 minutes,

(35) Longitudinal analysis of serum neutralization of SARS-CoV-2 Omicron BA.2, BA.4, and BA.5 in patients receiving monoclonal antibodiesCell Reports MedicineDecember 20, 2022Timothée Bruel, Karl Stéfic, Yann Nguyen, Donatella Toniutti, Isabelle Staropoli, Françoise Porrot, Florence Guivel-Benhassine, William-Henry Bolland, Delphine Planas, Jérôme Hadjadj, et al.assays,Critical commercial assays,Critical commercial assays ,, ADCC Reporter Bioassay,Promega,Cat#G7010 Bright-Glo Luciferase Assay System,Promega,Cat#E2620 ,, Deposited data,Deposited data,Deposited data ,, D614G(hCoV-19/France/GE1973/2020),GISAID,EPI_ISL_41463 Delta,GISAID,EPI_ISL_2029113 Omicron

(36) Specific location of galactosylation in an afucosylated antiviral monoclonal antibody affects its FcÎ³RIIIA binding affinityFrontiers in ImmunologyOctober 12, 2022Grayson Hatfield, Lioudmila Tepliakova, Genevieve Gingras, Andrew Stalker, Xuguang Li, Yves Aubin, Roger Y. Tamglycoform analogues ADCC assays were performed using the ADCC Reporter Bioassay, V Variant (Promega, Cat #G7010). To adapt it to the RSV model, Hep2 cells were first cultured in a 96-well plate at a cell density

(37) Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1PharmaceuticsJune 29, 2022Mohan N, Agrawal A, Shen Y, Winarski KL, Endo Y, Dokmanovic M, Schmiel D, Zheng J, Rotstein DS, Pelosof LC, et al.concentrations. 2.7. ADCC Reporter Bioassay The ADCC Reporter Bioassay developed by Promega (cat# G7010) is a modified version of the classic ADCC assay in which Jurkat effector cells are engineered to stably

(38) IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomesCell reportsMay 31, 2022Garrido JL, Medina MA, Bravo F, McGee S, Fuentes-Villalobos F, Calvo M, Pinos Y, Bowman JW, Bahl CD, Barria MI, et al.lines ,, 293T cells,ATCC,CRL-3216 "FcγRIIa and FcγRIIIa, CD4+ Jurkat reporter cell lines",Promega,G7010 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, "SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-FLAG tag (Codon Optimized

(39) Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Hyperimmune Immunoglobulin Demonstrates Potent Neutralization and Antibody-Dependent Cellular Cytotoxicity and Phagocytosis Through N and S ProteinsThe Journal of infectious diseasesMarch 15, 2022Díez JM, Romero C, Cruz M, Vandeberg P, Merritt WK, Pradenas E, Trinité B, Blanco J, Clotet B, Willis T, et al.ADCC Reporter Bioassay, Core Kit, Promega (ADCC Reporter Bioassays, FcγRIIIa V158 variant [high affinity], catalog numbers G7010 and G7018; Promega Corporation); and FcγRIIa-H (high affinity) ADCP Bioassay, Core Kit, Promega (ADCP

(40) Broadly neutralizing antibodies target a haemagglutinin anchor epitopeNatureFebruary 1, 2022Guthmiller JJ, Han J, Utset HA, Li L, Lan LY, Henry C, Stamper CT, McMahon M, O'Dell G, Fernández-Quintero ML, et al.(Invitrogen). Jurkat cells expressing FcgRIIIa and FcgRI with a NFAT-driven luciferase reporter gene (#G7010) were acquired and validated by Promega and were directly used for antibody-dependent cellular cytotoxicity

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ADCC Reporter Bioassay, Core Kit

ADCC Reporter Bioassay, Core Kit from Promega

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ADCC Reporter Bioassay, Core Kit from Promega

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