Maxwell RSC simplyRNA Tissue Kit AS1340 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Tissue Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1340
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeTissue samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti-CD47-induced macrophage phagocytosis via NF-kB-TGM2 driven tumor inflammationJournal for immunotherapy of cancerJanuary 1, 2023Marquardt V, Theruvath J, Pauck D, Picard D, Qin N, Blümel L, Maue M, Bartl J, Ahmadov U, Langini M, et al.using Trizol (#15 596–018, Thermo Scientific) or the Maxwell RSC Instrument (RSC simplyRNA Tissue, #AS1340, Promega), and complementary DNA (cDNA) was synthesized from 0.5 µg RNA using M-MLV Reverse Transcriptase

(82) Glycoprotein (GP)96 Is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal CapacityCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Häfliger J, Schwarzfischer M, Atrott K, Stanzel C, Morsy Y, Wawrzyniak M, Lang S, Valenta T, Basler K, Rogler G, et al.cells were either frozen for protein isolation or resuspended in Maxwell RNA homogenization solution (AS1340; Promega) for RNA purification and stored at -80°C until further processing. RNA Isolation, Complementary

(83) Ataxia-Telangiectasia Mutated Loss of Heterozygosity in MelanomaInternational Journal of Molecular SciencesDecember 16, 2022Lorenza Pastorino, Bruna Dalmasso, Eleonora Allavena, Irene Vanni, Filippo Ugolini, Gianna Baroni, Michela Croce, Antonio Guadagno, Francesco Cabiddu, Virginia Andreotti, et al.RNA Sequencing RNA from fresh tissue was extracted by the Tissue Lyser plus Maxwell® RSC simplyRNA Tissue Kit (AS1340 Promega, Southampton, UK) in accordance with the manufacturer. Total RNA concentration was quantified

(84) Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculationNature communicationsDecember 12, 2022Jeong GU, Kwon HJ, Ng WH, Liu X, Moon HW, Yoon GY, Shin HJ, Lee IC, Ling ZL, Spiteri AG, et al.USA). Quantitative RT-PCR Total RNA was extracted from homogenates using Maxwell RSC simplyRNA tissue kit (AS1340, Promega, Madison, WI, USA) following the manufacturer’s protocol. Quantitative RT-PCR (QuantStudio

(85) RNA-Seq comparative study reveals molecular effectors linked to the resistance of Pinna nobilis to Haplosporidium pinnae parasiteScientific ReportsDecember 8, 2022Pauline Salis, Claire Peyran, Titouan Morage, Simon de Bernard, Julien Nourikyan, Stéphane Coupé, Robert Bunet, Serge PlanesMaxwell® RSC Instrument (Promega, reference: AS4500) and Maxwell® RSC simplyRNA Tissue Kit (Promega-reference: AS1340). RNA‐Seq libraries were generated with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) from

(86) A derived honey bee stock confers resistance to Varroa destructor and associated viral transmissionScientific ReportsDecember 1, 2022Thomas A. O’Shea-Wheller, Frank D. Rinkevich, Robert G. Danka, Michael Simone-Finstrom, Philip G. Tokarz, Kristen B. Healycarried out using the Maxwell RSC simplyRNA tissue protocol (Promega) (Maxwell RSC simplyRNA Tissue Kit, AS1340, TM416, 2019). Sample purity and yield were then calculated in duplicate, using a NanoDrop One microvolume

(87) Elevation of cell-associated HIV-1 transcripts in CSF CD4+ T cells, despite effective antiretroviral therapy, is linked to brain injuryProceedings of the National Academy of SciencesNovember 29, 2022Kazuo Suzuki, John Zaunders, Thomas M. Gates, Angelique Levert, Shannen Butterly, Zhixin Liu, Takaomi Ishida, Sarah Palmer, Caroline D. Rae, Lauriane Jugé, et al.Maxwell RSC Buffy Coat DNA kit (Cat No. AS1540, Promega) and Maxwell RSC Simply RNA Tissue kit (Cat No. AS1340, Promega), respectively, according to the manufacturer’s protocol. The RNA assay for PBMCs uses the

(88) Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx miceNucleic acids researchNovember 11, 2022Desjardins CA, Yao M, Hall J, O'Donnell E, Venkatesan R, Spring S, Wen A, Hsia N, Shen P, Russo R, et al.The Maxwell RSC instrument (Cat. No. AS8500) and Maxwell RSC simplyTissue extraction kit (Cat. No. AS1340) were obtained from Promega, Madison, WI, USA. LabChip GXII Touch HT (CLS138160) and the DNA 5K/RNA/Charge

(89) Identification of the Transcription Factor ATF3 as a Direct and Indirect Regulator of the LDLRMetabolitesSeptember 6, 2022Sabine Bauer et al.from Hep3B cells using a modified protocol of the semi-automated Maxwell® RSC simply RNA tissue kit (AS1340 Promega©). The in-house established protocol included mechanical (Tissue Grinder, NIPPON Genetics EUROPE,

(90) Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencingRedox biologySeptember 1, 2022Pontel LB, Bueno-Costa A, Morellato AE, Carvalho Santos J, Roué G, Esteller M.mRNA expression. Briefly, total RNA was extracted from cell pellets using the SimplyRNA kit (Promega #AS1340) in the automated Maxwell RSC device (Promega, #AS4500). 2 μg of total RNA were converted to cDNA using

(91) The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumorsNature communicationsJuly 13, 2022Bartl J, Zanini M, Bernardi F, Forget A, Blümel L, Talbot J, Picard D, Qin N, Cancila G, Gao Q, et al.(Thermo Fisher Scientific, #15596018) according to the manufacturer’s instructions or by using the MaxWell system (Promega, #AS1340). Isolated RNA was quantified by spectrophotometry and 500 ng of RNA was retrotranscribed to total cDNA

(92) Selective activation and expansion of regulatory T cells using lipid encapsulated mRNA encoding a long-acting IL-2 muteinNature communicationsJuly 5, 2022de Picciotto S, DeVita N, Hsiao CJ, Honan C, Tse SW, Nguyen M, Ferrari JD, Zheng W, Wipke BT, Huang E.Maxwells with the RSC simplyRNA Tissue Kit as per the manufacturer’s recommendations (Promega, cat #AS1340). RNA quality was assessed using Agilent Tape Station RNA ScreenTape (Agilent, cat #5067-5576) and quantitated

(93) TAS2R38 Bitter Taste Receptor Expression in Chronic Rhinosinusitis with Nasal Polyps: New Data on Polypoid TissueInternational Journal of Molecular SciencesJuly 1, 2022Joanna Jeruzal-Świątecka, Edyta Borkowska, Mateusz Łaszczych, Zuzanna Nowicka, Wioletta Pietruszewskafrom nasal mucosa biopsies using Maxwell® RSC simplyRNA Tissue Kit (Promega, Madison, WI, USA; cat No AS1340). The RNA was DNAse treated with TURBO DNA-freeTM Kit (Ambion, Thermofisher, Waltham, MA, USA; cat No

(94) DNA Methylation Regulates a Set of Long Non-Coding RNAs Compromising Hepatic Identity during HepatocarcinogenesisCancers (Basel)April 19, 2022Miriam Recalde et al.was isolated using The Maxwell® RSC simplyRNA Tissue Kit in the Maxwell RSC 48 Instrument (Promega; AS1340; Madison, WI, USA). RNA samples were treated with DNase to degrade all possible traces of contaminating

(95) Sustained Downregulation of Vascular Smooth Muscle Acta2 After Transient Angiotensin II Infusion: A New Model of “Vascular Memory”Frontiers in Cardiovascular MedicineMarch 14, 2022Lucie Pothen et al.between passages 4 and 8 were used for experiments. Total RNA was extracted with Maxwell Kit (Promega, #AS1340). Extracted RNA was reverse-transcribed and analyzed by quantitative polymerase chain reaction (qPCR)

(96) Chlamydia pneumoniae can infect the central nervous system via the olfactory and trigeminal nerves and contributes to Alzheimer's disease riskScientific reportsFebruary 17, 2022Chacko A, Delbaz A, Walkden H, Basu S, Armitage CW, Eindorf T, Trim LK, Miller E, West NP, St John JA, et al.control mice (N = 3 for all groups) was extracted using Maxwell® RSC simplyRNA tissue kits (Promega, AS1340) using the manufacturer’s protocol. RNA was eluted in 50 µL of nuclease-free water and quality/quantity

(97) Side-by-Side Comparison of Post-Entry Quarantine and High Throughput Sequencing Methods for Virus and Viroid DiagnosisBiology (Basel)February 8, 2022Marie-Emilie A. Gauthier, Ruvini V. Lelwala, Candace E. Elliott, Craig Windell, Sonia Fiorito, Adrian Dinsdale, Mark Whattam, Julie Pattemore, Roberto A. BarreroConcentrator (RSC; Promega, Madisson, WI, USA) instrument using Maxwell® RSC SimplyRNA Tissue kit (AS1340, Promega). In brief, tissue samples were placed with a sterile 7 mm stainless steel bead in a sterile

(98) Liraglutide + PYY3-36 Combination Therapy Mimics Effects of Roux-en-Y Bypass on Early NAFLD Whilst Lacking-Behind in Metabolic ImprovementsJournal of Clinical MedicineJanuary 30, 2022Valentin Metzner, Gloria Herzog, Tobias Heckel, Thorsten Bischler, Julia Hasinger, Christoph Otto, Martin Fassnacht, Andreas Geier, Florian Seyfried, Ulrich Dischingermacrodissected in 10 mg organ samples. Total RNA was extracted using the Maxwell® RSC simplyRNA Tissue Kit (AS1340; Promega, Fitchburg, WI, USA) and the Maxwell® RSC Instrument (AS4500; Promega). Prior to extraction,

(99) Optimising the IgG-degrading enzyme treatment regimen for enhanced adeno-associated virus transduction in the presence of neutralising antibodiesClinical & translational immunologyJanuary 1, 2022Ros-Gañán I, Hommel M, Trigueros-Motos L, Tamarit B, Rodríguez-García E, Salas D, Pérez G, Douar A, Combal JP, Benichou B, et al.the manufacturer´s protocol. Total RNA was extracted using the Maxwell® 16 LEV simplyRNA Tissue Kit (#AS1340; Promega) according to the manufacturer's instructions, and 1 μg RNA was reverse transcribed into complementary

(100) Roux-en-Y Gastric Bypass and Caloric Restriction but Not Gut Hormone-Based Treatments Profoundly Impact the Hypothalamic Transcriptome in Obese RatsNutrientsDecember 28, 2021Ulrich Dischinger, Tobias Heckel, Thorsten Bischler, Julia Hasinger, Malina Königsrainer, Angelika Schmitt-Böhrer, Christoph Otto, Martin Fassnacht, Florian Seyfried, Mohammed Khair HankirPromega, Fitchburg, WI, USA). Total RNA was extracted using Promega’s “Maxwell Simply RNA Tissue Kit” (AS1340) and measured using a NanoDrop™ 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). 2.5.

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Maxwell RSC simplyRNA Tissue Kit

Maxwell RSC simplyRNA Tissue Kit from Promega

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Maxwell RSC simplyRNA Tissue Kit from Promega

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