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Maxwell RSC simplyRNA Tissue Kit
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Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit from Promega
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Citations:
(133)
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Maxwell RSC simplyRNA Tissue Kit
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Description
The Maxwell RSC simplyRNA Tissue Kit, used with the Maxwell Instruments, provides a simple method for automated RNA extraction from up to 20mg tissue samples
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC simplyRNA Tissue Kit
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS1340
Quantity
48 preps
Source
Cell/Tissue
Nucleic Acid Type
Total RNA
Sample Type
Tissue samples
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (133)
(81)
Unravelling homologous recombination repair deficiency and therapeutic opportunities in soft tissue and bone sarcoma
EMBO Molecular Medicine
April 11, 2023
Lara Planas‐Paz, Alicia Pliego‐Mendieta, Catherine Hagedorn, Domingo Aguilera‐Garcia, Martina Haberecker, Fabian Arnold, Marius Herzog, Lorenz Bankel, Roman Guggenberger, Sabrina Steiner,
et al.
patient‐derived
sarcoma
cell models was extracted using the
Maxwell RSC simplyRNA Tissue kit
(
Promega
AS1340
). The extracted RNA was quantified with a Nanodrop (ThermoFisher Scientific) and the High
Sensitivity
(82)
Prognostic Value and Genome Signature of m6A/m5C Regulated Genes in Early-Stage Lung Adenocarcinoma
International Journal of Molecular Sciences
March 30, 2023
Long Tian, Yan Wang, Jie Tian, Wenpeng Song, Lu Li, Guowei Che
paracancerous tissues of LUAD was extracted using the Maxwell® RSC simply RNA Tissue Kit RNA (
Promega
, cat:
AS1340
) following the manufacturer’s protocol. According to the manufacturer’s instructions, the reverse transcription
(83)
mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
Cell reports. Medicine
March 17, 2023
Olivera I, Bolaños E, Gonzalez-Gomariz J, Hervas-Stubbs S, Mariño KV, Luri-Rey C, Etxeberria I, Cirella A, Egea J, Glez-Vaz J,
et al
.
ProcartaPlex Mix&Match 13-plex,Thermofisher,Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue,
Promega
,Cat#
AS1340
,, Deposited data,Deposited data,Deposited data ,, RNAseq data,This paper,GEO: GSE206195 ,,
Experimental models
(84)
Inflammation and neuronal gene expression changes differ in early versus late chronic traumatic encephalopathy brain
BMC Medical Genomics
March 9, 2023
Adam Labadorf, Filisia Agus, Nurgul Aytan, Jonathan Cherry, Jesse Mez, Ann McKee, Thor D. Stein
9 gyral crest. Total RNA was extracted using the
Promega
Maxwell RSC simplyRNA Tissue Kit
(Cat No.
AS1340
) according to the manufacturer’s protocol. The integrity and quality of RNA were verified by an
Agilent
(85)
The Potent G-Quadruplex-Binding Compound QN-302 Downregulates S100P Gene Expression in Cells and in an In Vivo Model of Pancreatic Cancer
Molecules
March 7, 2023
Ahmed A. Ahmed, William Greenhalf, Daniel H. Palmer, Nicole Williams, Jenny Worthington, Tariq Arshad, Shozeb Haider, Effrosyni Alexandrou, Dilek Guneri, Zoe A. E. Waller,
et al.
pancreatic
tumor
tissue with the
Maxwell® RSC SimplyRNA Tissue Kit
(
Promega
Ltd. Loughborough, UK, Cat #
AS1340
). In brief, the tissue was snap frozen in the operating theatre following
tumor
removal and then homogenized
(86)
Conditional ablation of heparan sulfate expression in stromal fibroblasts promotes tumor growth in vivo
PLOS ONE
February 21, 2023
Ayumi Niwa, Toshiaki Taniguchi, Hiroyuki Tomita, Hideshi Okada, Takamasa Kinoshita, Chika Mizutani, Mikiko Matsuo, Yuko Imaizumi, Takahito Kuroda, Koki Ichihashi,
et al.
transplanted
tumors
using a
Maxwell RSC simplyRNA Tissue Kit
(
Promega
Corp., Fitchburg, WI, USA; cat. #
AS1340
). Using the Low Input Quick Amp
Labeling
Kit 1-color (
Agilent Technologies
, Santa Clara, CA, USA; #
5190–2305
),
(87)
Identification of germline cancer predisposition variants in pediatric sarcoma patients from somatic tumor testing
Scientific Reports
February 20, 2023
Piedad Alba-Pavón, Lide Alaña, Miriam Gutierrez-Jimeno, Susana García-Obregón, Teresa Imízcoz, Elena Panizo, Paula González-Urdiales, Aizpea Echebarria-Barona, Ricardo Lopez Almaraz, Laura Zaldumbide,
et al.
(
Promega
,
AS1610
),
Maxwell RSC RNA FFPE kit
(
Promega
,
AS1440
) and Maxwell RSC Simply RNA tissue (
Promega
,
AS1340
). DNA and RNA were quantified using a
Qubit fluorometer
and adjusted to a final quantity of 50 ng of
(88)
Integrative multi-omics networks identify PKCδ and DNA-PK as master kinases of glioblastoma subtypes and guide targeted cancer therapy
Nature Cancer
February 2, 2023
Simona Migliozzi, Young Taek Oh, Mohammad Hasanain, Luciano Garofano, Fulvio D’Angelo, Ryan D. Najac, Alberto Picca, Franck Bielle, Anna Luisa Di Stefano, Julie Lerond,
et al.
Maxwell Rapid Sample Concentrator Instrument (Promega) and
Maxwell RSC simplyRNA Tissue Kit
(
Promega
,
AS1340
) for frozen samples or
Maxwell RSC RNA FFPE kit
(
Promega
,
AS1440
) for FFPE specimens. RNA extracted
(89)
Low-Intensity Pulsed Ultrasound-Mediated Blood-Brain Barrier Opening Increases Anti-Programmed Death-Ligand 1 Delivery and Efficacy in Gl261 Mouse Model
Pharmaceutics
January 30, 2023
Ahmed MH, Hernández-Verdin I, Quissac E, Lemaire N, Guerin C, Guyonnet L, Zahr N, Mouton L, Santin M, Petiet A,
et al
.
brain tissues. mRNA was extracted using Maxwell RSC simply RNA automated
RNA purification kit
(
Promega
,
AS1340
). RNA quality was analyzed using high-
sensitivity
RNA chips. For
RNA sequencing
, NovaSeq 6000 sequencer
(90)
Cellular senescence in malignant cells promotes tumor progression in mouse and patient Glioblastoma
Nature Communications
January 27, 2023
Rana Salam, Alexa Saliou, Franck Bielle, Mathilde Bertrand, Christophe Antoniewski, Catherine Carpentier, Agusti Alentorn, Laurent Capelle, Marc Sanson, Emmanuelle Huillard,
et al.
either the TRI-reagent (Molecular Center Research, #TR 118), the Maxwell RSC simplyRNA Tissue (
Promega
, #
AS1340
), and the Macherey-Nagel Mini kit Nucleospin protocol (Macherey-Nagel, #740955.50). cDNAs were generated
(91)
Exploration of Patient-Derived Pancreatic Ductal Adenocarcinoma Ex Vivo Tissue for Treatment Response
Antioxidants (Basel)
January 11, 2023
Tímea Szekerczés, Arun Kumar Selvam, Carlos Fernández Moro, Soledad Pouso Elduayen, Joakim Dillner, Mikael Björnstedt, Mehran Ghaderi
Extraction and Two-Step
Real-Time PCR
Total-RNA was isolated using
Maxwell RSC simplyRNA Tissue Kit
(
AS1340
,
Promega Corporation
, Madison, WI, USA) by
Maxwell RSC Instrument
(
Promega Corporation
, Madison, WI,
(92)
Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti-CD47-induced macrophage phagocytosis via NF-kB-TGM2 driven tumor inflammation
Journal for immunotherapy of cancer
January 1, 2023
Marquardt V, Theruvath J, Pauck D, Picard D, Qin N, Blümel L, Maue M, Bartl J, Ahmadov U, Langini M,
et al
.
using Trizol (#15 596–018, Thermo Scientific) or the
Maxwell RSC Instrument
(RSC simplyRNA Tissue, #
AS1340
,
Promega
), and complementary DNA (cDNA) was synthesized from 0.5 µg RNA using
M-MLV Reverse Transcriptase
(93)
Ataxia-Telangiectasia Mutated Loss of Heterozygosity in Melanoma
International Journal of Molecular Sciences
December 16, 2022
Lorenza Pastorino, Bruna Dalmasso, Eleonora Allavena, Irene Vanni, Filippo Ugolini, Gianna Baroni, Michela Croce, Antonio Guadagno, Francesco Cabiddu, Virginia Andreotti,
et al.
RNA Sequencing
RNA from fresh tissue was extracted by the Tissue Lyser plus
Maxwell® RSC simplyRNA Tissue Kit
(
AS1340
Promega
, Southampton, UK) in accordance with the manufacturer. Total RNA concentration was quantified
(94)
Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation
Nature communications
December 12, 2022
Jeong GU, Kwon HJ, Ng WH, Liu X, Moon HW, Yoon GY, Shin HJ, Lee IC, Ling ZL, Spiteri AG,
et al
.
USA). Quantitative
RT-PCR
Total RNA was extracted from homogenates using
Maxwell RSC simplyRNA tissue kit
(
AS1340
,
Promega
, Madison, WI, USA) following the manufacturer’s protocol. Quantitative
RT-PCR
(QuantStudio
(95)
RNA-Seq comparative study reveals molecular effectors linked to the resistance of Pinna nobilis to Haplosporidium pinnae parasite
Scientific Reports
December 8, 2022
Pauline Salis, Claire Peyran, Titouan Morage, Simon de Bernard, Julien Nourikyan, Stéphane Coupé, Robert Bunet, Serge Planes
Maxwell® RSC Instrument
(
Promega
, reference:
AS4500
) and
Maxwell® RSC simplyRNA Tissue Kit
(
Promega
-reference:
AS1340
).
RNA‐Seq
libraries were generated with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) from
(96)
A derived honey bee stock confers resistance to Varroa destructor and associated viral transmission
Scientific Reports
December 1, 2022
Thomas A. O’Shea-Wheller, Frank D. Rinkevich, Robert G. Danka, Michael Simone-Finstrom, Philip G. Tokarz, Kristen B. Healy
carried out using the Maxwell RSC simplyRNA tissue protocol (
Promega
) (
Maxwell RSC simplyRNA Tissue Kit
,
AS1340
, TM416, 2019). Sample purity and yield were then calculated in duplicate, using a NanoDrop One microvolume
(97)
Elevation of cell-associated HIV-1 transcripts in CSF CD4+ T cells, despite effective antiretroviral therapy, is linked to brain injury
Proceedings of the National Academy of Sciences
November 29, 2022
Kazuo Suzuki, John Zaunders, Thomas M. Gates, Angelique Levert, Shannen Butterly, Zhixin Liu, Takaomi Ishida, Sarah Palmer, Caroline D. Rae, Lauriane Jugé,
et al.
Maxwell RSC Buffy Coat DNA kit
(Cat No.
AS1540
,
Promega
) and Maxwell RSC Simply RNA Tissue kit (Cat No.
AS1340
,
Promega
), respectively, according to the manufacturer’s protocol. The RNA
assay
for PBMCs uses the
(98)
Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice
Nucleic acids research
November 11, 2022
Desjardins CA, Yao M, Hall J, O'Donnell E, Venkatesan R, Spring S, Wen A, Hsia N, Shen P, Russo R,
et al
.
The
Maxwell RSC instrument
(Cat. No.
AS8500
) and Maxwell RSC simplyTissue extraction kit (Cat. No.
AS1340
) were obtained from
Promega
, Madison, WI, USA. LabChip GXII Touch HT (CLS138160) and the DNA 5K/RNA/Charge
(99)
Identification of the Transcription Factor ATF3 as a Direct and Indirect Regulator of the LDLR
Metabolites
September 6, 2022
Sabine Bauer et al.
from Hep3B cells using a modified protocol of the semi-automated Maxwell® RSC simply RNA tissue kit (
AS1340
Promega
©). The in-house established protocol included mechanical (Tissue Grinder, NIPPON Genetics EUROPE,
(100)
Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing
Redox biology
September 1, 2022
Pontel LB, Bueno-Costa A, Morellato AE, Carvalho Santos J, Roué G, Esteller M.
mRNA expression. Briefly, total RNA was extracted from cell pellets using the SimplyRNA kit (
Promega
#
AS1340
) in the automated Maxwell RSC device (
Promega
, #
AS4500
). 2 μg of total RNA were converted to cDNA using
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Maxwell RSC simplyRNA Tissue Kit from Promega
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