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Maxwell RSC simplyRNA Tissue Kit
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Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit from Promega
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Citations:
(133)
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Maxwell RSC simplyRNA Tissue Kit
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Description
The Maxwell RSC simplyRNA Tissue Kit, used with the Maxwell Instruments, provides a simple method for automated RNA extraction from up to 20mg tissue samples
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC simplyRNA Tissue Kit
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS1340
Quantity
48 preps
Source
Cell/Tissue
Nucleic Acid Type
Total RNA
Sample Type
Tissue samples
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (133)
(61)
SGLT2 inhibition eliminates senescent cells and alleviates pathological aging
Nat Aging
July 1, 2024
Katsuumi G, Shimizu I, Suda M, Yoshida Y, Furihata T, Joki Y, Hsiao CL, Jiaqi L, Fujiki S, Abe M,
et al
.
using the
Maxwell RSC 48 Instrument
(
Promega
) and
Maxwell RSC simplyRNA Tissue Kit
(
Promega
, cat. no.
AS1340
) in accordance with the manufacturer’s instructions. The complementary DNA libraries were generated
(62)
Phosphatidylcholine levels regulate hyphal elongation and differentiation in the filamentous fungus Aspergillus oryzae
Scientific Reports
May 22, 2024
Tetsuki Suzawa, Ryo Iwama, Ryouichi Fukuda, Hiroyuki Horiuchi
metal crusher was removed, and total RNAs were extracted using a
Maxwell RSC simplyRNA Tissue Kit
(
AS1340
;
Promega
K.K., Tokyo, Japan). Reverse transcription of RNA was performed using a ReverTra Ace qPCR RT
(63)
Combining germline, tissue and liquid biopsy analysis by comprehensive genomic profiling to improve the yield of actionable variants in a real-world cancer cohort
Journal of Translational Medicine
May 15, 2024
I. Vanni, L. Pastorino, V. Andreotti, D. Comandini, G. Fornarini, M. Grassi, A. Puccini, E. T. Tanda, A. Pastorino, V. Martelli,
et al.
USA) while the somatic RNA was extracted by the Tissue Lyser plus
Maxwell® RSC simplyRNA Tissue Kit
(
AS1340
Promega
, Southampton, UK) in accordance with the manufacturer. Quantity and purity of the
tumor
and
(64)
Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors
Experimental and therapeutic medicine
April 1, 2024
Sun Z, Fukui M, Taketani S, Kako A, Kunieda S, Kakudo N.
version 1.53t).
Gene expression
Total RNA was isolated from the hASCs using a Maxwell RSC kit (cat. no.
AS1340
;
Promega Corporation
) (25). Reverse transcription-
quantitative PCR
(
RT-qPCR
) was performed using SYBR
(65)
Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B
Sci Rep
March 5, 2024
Misaki Okahata, Natsumi Sawada, Kenji Nakao, Akane Ohta, Atsushi Kuhara
20 h, the animals were transferred to 2 °C for 3 h. The Maxwell RSC Simply RNA tissue kit (
Promega
;
AS1340
) with
Maxwell RSC Instrument
(
Promega
;
AS4500
) was used to extract total RNA in accordance with the
(66)
Identification of SARS-CoV-2 variants in indoor dust
PLOS ONE
February 9, 2024
John Van Dusen, Haley LeBlanc, Nicholas Nastasi, Jenny Panescu, Austin Shamblin, Jacob W. Smith, Michael G. Sovic, Amanda Williams, Mikkel B. M. Quam, Seth Faith,
et al.
analysis. After September 2021, RNA was extracted from dust samples using the SimplyRNA Tissue kit (
AS1340
,
Promega
Co. Madison, WI) with a Promega Maxwell RSC 48 sample concentrator with improved RNA recovery.
(67)
Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar Tissues
International Journal of Molecular Sciences
February 9, 2024
Junya Kusumoto, Masaya Akashi, Hiroto Terashi, Shunsuke Sakakibara
cultured cells
using the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) and the Maxwell RSC simplyRNA kit (
AS1340
,
Promega
), following the manufacturer’s instructions. Briefly, the cells were detached using Accutase
(68)
Semaphorin 3C promotes de novo steroidogenesis in prostate cancer cells
Endocrine-related cancer
December 1, 2023
Yenki P, Bhasin S, Liu L, Nabavi N, Cheng CW, Tam KJ, Peacock JW, Adomat HH, Tombe T, Fazli L,
et al
.
LNCaPvector (n = 3 per group) was isolated with Maxwell® RSC simply RNA Tissue Kit (
Promega
, Cat. No.
AS1340
) using
Maxwell RSC Instrument
(
Promega
, Cat. No.
AS4500
). Following quality control by TapeStation system,
(69)
A complex interplay between H2A.Z and HP1 isoforms regulates pericentric heterochromatin
Frontiers in Cell and Developmental Biology
November 9, 2023
Jessica González, Laia Bosch-Presegué, Anna Marazuela-Duque, Anna Guitart-Solanes, María Espinosa-Alcantud, Agustín F. Fernandez, Jeremy P. Brown, Juan Ausió, Berta N. Vazquez, Prim B. Singh,
et al.
cDNA synthesis
, and
RT-qPCR
Total RNA was purified using
Maxwell RSC simplyRNA Tissue kit
(
Promega
,
AS1340
). The cDNA was synthesized from 3 μg of total RNA with a
Transcriptor First Strand cDNA Synthesis Kit
(70)
Cont-ID: detection of sample cross-contamination in viral metagenomic data
BMC Biology
October 13, 2023
Johan Rollin, Wei Rong, Sébastien Massart
(mix),Total nucleic acid,"Maxwell® Rapid Sample Concentrator instrument using SimplyRNA Tissue kit (
AS1340
,
Promega
)",TruSeq Stranded Total RNA,Illumina NovaSeq 6000 2X150,BioProject: PRJNA752836,"Gauthier et
(71)
Characterization of mRNA Signature in Milk Small Extracellular Vesicles from Cattle Infected with Bovine Leukemia Virus
Pathogens
October 13, 2023
Md. Matiur Rahman, Hinata Ishikawa, Marika Yamauchi, Shigeo Takashima, Yuji O. Kamatari, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
cDNA Synthesis
The total RNA was obtained from milk sEVs using a Maxwell RSC simply RNA Tissue Kit (
AS1340
,
Promega
) according to the manufacturer’s instructions. The concentration of RNA was measured by NanoDrop
(72)
OGR1 (GPR68) and TDAG8 (GPR65) Have Antagonistic Effects in Models of Colonic Inflammation
International journal of molecular sciences
October 3, 2023
Perren L, Busch M, Schuler C, Ruiz PA, Foti F, Weibel N, de Vallière C, Morsy Y, Seuwen K, Hausmann M,
et al
.
isolated from colon and ileum using the
Maxwell RSC simplyRNA tissue kit
(
Promega
, Madison, WI, USA,
AS1340
). For all samples, lysis buffer from the kit was added to snap frozen resections, and samples were shredded
(73)
Integrative multi-omics reveals two biologically distinct groups of pilocytic astrocytoma
Acta Neuropathologica
October 1, 2023
Daniel Picard, Jörg Felsberg, Maike Langini, Paweł Stachura, Nan Qin, Jadranka Macas, Yvonne Reiss, Jasmin Bartl, Florian Selt, Romain Sigaud,
et al.
was isolated from the fresh frozen PA tissue samples using the Maxwell® RSC simply RNA Tissue Kit (
AS1340
,
Promega
, Walldorf, Germany). To prepare the barcoded libraries, 500 ng total RNA was processed using
(74)
Translocator protein (18kDA) (TSPO) marks mesenchymal glioblastoma cell populations characterized by elevated numbers of tumor-associated macrophages
Acta Neuropathologica Communications
September 11, 2023
Lorraine Weidner, Julia Lorenz, Stefanie Quach, Frank K. Braun, Tanja Rothhammer-Hampl, Laura-Marie Ammer, Arabel Vollmann-Zwerenz, Laura M. Bartos, Franziska J. Dekorsy, Adrien Holzgreve,
et al.
analysis RNA for
Next Generation sequencing (NGS
) was isolated using Maxwell® RSC Simply RNA Tissue kit (
AS1340
,
Promega
) according to manufacturer’s instructions.
NGS
libraries were prepared from 10 ng total RNA
(75)
Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic mice
Frontiers in Cardiovascular Medicine
August 16, 2023
Masayoshi Kukida, Naofumi Amioka, Dien Ye, Hui Chen, Jessica J. Moorleghen, Ching-Ling Liang, Deborah A. Howatt, Yuriko Katsumata, Motoko Yanagita, Hisashi Sawada,
et al.
isolation and
quantitative PCR
Total RNA of the renal cortex was extracted using a commercial kit (Cat #
AS1340
,
Promega
) configured with a
Maxwell RSC Instrument
(
Promega
). To quantify mRNA abundance, total RNA
(76)
Gene expression changes in Tay–Sachs disease begin early in fetal brain development
Journal of Inherited Metabolic Disease
July 1, 2023
Sangwoo T. Han, Ashley Hirt, Elena‐Raluca Nicoli, Mari Kono, Camilo Toro, Richard L. Proia, Cynthia J. Tifft
extracted from tissue samples using the
Maxwell® RSC simplyRNA Tissue Kit
(
Promega
, Madison, WI, USA, Cat #
AS1340
) according to manufacturer’s protocol. RNA quality was assessed using a Bioanalyzer (Agilent Technologies,
(77)
The multi-level regulation of clownfish metamorphosis by thyroid hormones
Cell Reports
July 1, 2023
Natacha Roux, Saori Miura, Mélanie Dussenne, Yuki Tara, Shu-hua Lee, Simon de Bernard, Mathieu Reynaud, Pauline Salis, Agneesh Barua, Abdelhay Boulahtouf,
et al.
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Maxwell RSC SimplyRNA Tissue Kit
,
Promega
,
AS1340
Bioanalyser RNA Kit & Reagents,
Agilent
,
5067-1511
TruSeq Stranded mRNA Sample Preparation Kit,Illumina,20020595 KAPA
(78)
Effect of an intraoperative periradicular application of platelet-rich fibrin (PRF) on residual post-surgical neuropathic pain after disc herniation surgery: study protocol for NeuroPRF, a randomized controlled trial
Trials
June 19, 2023
Julien Todeschi, Guillaume Dannhoff, Andres Hugo Coca, Daniel Ionut Timbolschi, François Proust, François Lefebvre, Vincent Lelievre, Pierrick Poisbeau, Laurent Vallat, Eric Salvat,
et al.
20 °C for back-up. The second will be resuspended in 200 μL of 1-thiogycerol solution (
Promega
, ref.
AS1340
), vortexed and stored at – 20 °C for RNA extraction. Total RNA extraction will be performed on a Maxwell
(79)
MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer
Cell reports. Medicine
April 18, 2023
Alburquerque-Bejar JJ, Navajas-Chocarro P, Saigi M, Ferrero-Andres A, Morillas JM, Vilarrubi A, Gomez A, Mate JL, Munoz-Marmol AM, Romero OA,
et al
.
BigDye Terminator V3.1 Cycle Sequencing kit
,
Applied Biosystems
,Cat#
4337456
Maxwell RSC simply RNA Tissue kit,
Promega
,Cat#
AS1340
SYBR Green PCR Master Mix
,
Applied Biosystems
,Cat#
4309155
Halt Protease and Phosphatase Inhibitor Cocktail (100X
(80)
RANK is a poor prognosis marker and a therapeutic target in ER‐negative postmenopausal breast cancer
EMBO Molecular Medicine
April 11, 2023
Marina Ciscar, Eva M Trinidad, Gema Perez‐Chacon, Mansour Alsaleem, Maria Jimenez, Maria J Jimenez‐Santos, Hector Perez‐Montoyo, Adrian Sanz‐Moreno, Andrea Vethencourt, Michael Toss,
et al.
isolated from
tumor
pieces using TRIzol (Thermo Fisher Scientific) or
Maxwell® RSC simplyRNA Tissue Kit
(
AS1340
Promega
). One microgram of RNA was reverse‐transcribed into cDNA using 200 U Superscript II plus random
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Maxwell RSC simplyRNA Tissue Kit from Promega
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