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Maxwell RSC simplyRNA Tissue Kit
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Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit
Maxwell RSC simplyRNA Tissue Kit from Promega
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Citations:
(118)
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Maxwell RSC simplyRNA Tissue Kit
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Description
The Maxwell RSC simplyRNA Tissue Kit, used with the Maxwell Instruments, provides a simple method for automated RNA extraction from up to 20mg tissue samples
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC simplyRNA Tissue Kit
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS1340
Quantity
48 preps
Source
Cell/Tissue
Nucleic Acid Type
Total RNA
Sample Type
Tissue samples
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (118)
(61)
Translocator protein (18kDA) (TSPO) marks mesenchymal glioblastoma cell populations characterized by elevated numbers of tumor-associated macrophages
Acta Neuropathologica Communications
September 11, 2023
Lorraine Weidner, Julia Lorenz, Stefanie Quach, Frank K. Braun, Tanja Rothhammer-Hampl, Laura-Marie Ammer, Arabel Vollmann-Zwerenz, Laura M. Bartos, Franziska J. Dekorsy, Adrien Holzgreve,
et al.
analysis RNA for
Next Generation sequencing (NGS
) was isolated using Maxwell® RSC Simply RNA Tissue kit (
AS1340
,
Promega
) according to manufacturer’s instructions.
NGS
libraries were prepared from 10 ng total RNA
(62)
Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic mice
Frontiers in Cardiovascular Medicine
August 16, 2023
Masayoshi Kukida, Naofumi Amioka, Dien Ye, Hui Chen, Jessica J. Moorleghen, Ching-Ling Liang, Deborah A. Howatt, Yuriko Katsumata, Motoko Yanagita, Hisashi Sawada,
et al.
isolation and
quantitative PCR
Total RNA of the renal cortex was extracted using a commercial kit (Cat #
AS1340
,
Promega
) configured with a
Maxwell RSC Instrument
(
Promega
). To quantify mRNA abundance, total RNA
(63)
Gene expression changes in Tay–Sachs disease begin early in fetal brain development
Journal of Inherited Metabolic Disease
July 1, 2023
Sangwoo T. Han, Ashley Hirt, Elena‐Raluca Nicoli, Mari Kono, Camilo Toro, Richard L. Proia, Cynthia J. Tifft
extracted from tissue samples using the
Maxwell® RSC simplyRNA Tissue Kit
(
Promega
, Madison, WI, USA, Cat #
AS1340
) according to manufacturer’s protocol. RNA quality was assessed using a Bioanalyzer (Agilent Technologies,
(64)
TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseases
Redox biology
July 1, 2023
Kim J, Lim J, Yoo ID, Park S, Moon JS.
with
Maxwell® RSC RNA FFPE Kit
(
AS1440
,
Promega
, Madison, WI, USA),
Maxwell® RSC simplyRNA tissue kit
(
AS1340
,
Promega
), or
Maxwell® RSC simplyRNA cells kit
(
AS1390
,
Promega
) with
Maxwell® RSC Instrument
(
AS4500
,
(65)
The multi-level regulation of clownfish metamorphosis by thyroid hormones
Cell Reports
July 1, 2023
Natacha Roux, Saori Miura, Mélanie Dussenne, Yuki Tara, Shu-hua Lee, Simon de Bernard, Mathieu Reynaud, Pauline Salis, Agneesh Barua, Abdelhay Boulahtouf,
et al.
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Maxwell RSC SimplyRNA Tissue Kit
,
Promega
,
AS1340
Bioanalyser RNA Kit & Reagents,
Agilent
,
5067-1511
TruSeq Stranded mRNA Sample Preparation Kit,Illumina,20020595 KAPA
(66)
Effect of an intraoperative periradicular application of platelet-rich fibrin (PRF) on residual post-surgical neuropathic pain after disc herniation surgery: study protocol for NeuroPRF, a randomized controlled trial
Trials
June 19, 2023
Julien Todeschi, Guillaume Dannhoff, Andres Hugo Coca, Daniel Ionut Timbolschi, François Proust, François Lefebvre, Vincent Lelievre, Pierrick Poisbeau, Laurent Vallat, Eric Salvat,
et al.
20 °C for back-up. The second will be resuspended in 200 μL of 1-thiogycerol solution (
Promega
, ref.
AS1340
), vortexed and stored at – 20 °C for RNA extraction. Total RNA extraction will be performed on a Maxwell
(67)
MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer
Cell reports. Medicine
April 18, 2023
Alburquerque-Bejar JJ, Navajas-Chocarro P, Saigi M, Ferrero-Andres A, Morillas JM, Vilarrubi A, Gomez A, Mate JL, Munoz-Marmol AM, Romero OA,
et al
.
BigDye Terminator V3.1 Cycle Sequencing kit
,
Applied Biosystems
,Cat#
4337456
Maxwell RSC simply RNA Tissue kit,
Promega
,Cat#
AS1340
SYBR Green PCR Master Mix
,
Applied Biosystems
,Cat#
4309155
Halt Protease and Phosphatase Inhibitor Cocktail (100X
(68)
Synergistic effects of combined immunotherapy strategies in a model of multifocal hepatocellular carcinoma
Cell reports. Medicine
April 18, 2023
Ochoa MC, Sanchez-Gregorio S, de Andrea CE, Garasa S, Alvarez M, Olivera I, Glez-Vaz J, Luri-Rey C, Etxeberria I, Cirella A,
et al
.
assays
,Critical commercial
assays
,Critical commercial
assays
,, Maxwell RSC simplyRNA extraction kit,
Promega
,
AS1340
Endo-free
maxiprep
,Quiagen,12362 M-MLV enzyme,
Invitrogen
,
28025013
IFNγ ELISA KIT,
BD
OptEIA,
551866
"
CD8 + T cell Isolation Kit, human
(69)
RANK is a poor prognosis marker and a therapeutic target in ER‐negative postmenopausal breast cancer
EMBO Molecular Medicine
April 11, 2023
Marina Ciscar, Eva M Trinidad, Gema Perez‐Chacon, Mansour Alsaleem, Maria Jimenez, Maria J Jimenez‐Santos, Hector Perez‐Montoyo, Adrian Sanz‐Moreno, Andrea Vethencourt, Michael Toss,
et al.
isolated from
tumor
pieces using TRIzol (Thermo Fisher Scientific) or
Maxwell® RSC simplyRNA Tissue Kit
(
AS1340
Promega
). One microgram of RNA was reverse‐transcribed into cDNA using 200 U Superscript II plus random
(70)
Unravelling homologous recombination repair deficiency and therapeutic opportunities in soft tissue and bone sarcoma
EMBO Molecular Medicine
April 11, 2023
Lara Planas‐Paz, Alicia Pliego‐Mendieta, Catherine Hagedorn, Domingo Aguilera‐Garcia, Martina Haberecker, Fabian Arnold, Marius Herzog, Lorenz Bankel, Roman Guggenberger, Sabrina Steiner,
et al.
patient‐derived
sarcoma
cell models was extracted using the
Maxwell RSC simplyRNA Tissue kit
(
Promega
AS1340
). The extracted RNA was quantified with a Nanodrop (ThermoFisher Scientific) and the High
Sensitivity
(71)
Prognostic Value and Genome Signature of m6A/m5C Regulated Genes in Early-Stage Lung Adenocarcinoma
International Journal of Molecular Sciences
March 30, 2023
Long Tian, Yan Wang, Jie Tian, Wenpeng Song, Lu Li, Guowei Che
paracancerous tissues of LUAD was extracted using the Maxwell® RSC simply RNA Tissue Kit RNA (
Promega
, cat:
AS1340
) following the manufacturer’s protocol. According to the manufacturer’s instructions, the reverse transcription
(72)
mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
Cell reports. Medicine
March 17, 2023
Olivera I, Bolaños E, Gonzalez-Gomariz J, Hervas-Stubbs S, Mariño KV, Luri-Rey C, Etxeberria I, Cirella A, Egea J, Glez-Vaz J,
et al
.
ProcartaPlex Mix&Match 13-plex,Thermofisher,Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue,
Promega
,Cat#
AS1340
,, Deposited data,Deposited data,Deposited data ,, RNAseq data,This paper,GEO: GSE206195 ,,
Experimental models
(73)
Inflammation and neuronal gene expression changes differ in early versus late chronic traumatic encephalopathy brain
BMC Medical Genomics
March 9, 2023
Adam Labadorf, Filisia Agus, Nurgul Aytan, Jonathan Cherry, Jesse Mez, Ann McKee, Thor D. Stein
9 gyral crest. Total RNA was extracted using the
Promega
Maxwell RSC simplyRNA Tissue Kit
(Cat No.
AS1340
) according to the manufacturer’s protocol. The integrity and quality of RNA were verified by an
Agilent
(74)
The Potent G-Quadruplex-Binding Compound QN-302 Downregulates S100P Gene Expression in Cells and in an In Vivo Model of Pancreatic Cancer
Molecules
March 7, 2023
Ahmed A. Ahmed, William Greenhalf, Daniel H. Palmer, Nicole Williams, Jenny Worthington, Tariq Arshad, Shozeb Haider, Effrosyni Alexandrou, Dilek Guneri, Zoe A. E. Waller,
et al.
pancreatic
tumor
tissue with the
Maxwell® RSC SimplyRNA Tissue Kit
(
Promega
Ltd. Loughborough, UK, Cat #
AS1340
). In brief, the tissue was snap frozen in the operating theatre following
tumor
removal and then homogenized
(75)
Conditional ablation of heparan sulfate expression in stromal fibroblasts promotes tumor growth in vivo
PLOS ONE
February 21, 2023
Ayumi Niwa, Toshiaki Taniguchi, Hiroyuki Tomita, Hideshi Okada, Takamasa Kinoshita, Chika Mizutani, Mikiko Matsuo, Yuko Imaizumi, Takahito Kuroda, Koki Ichihashi,
et al.
transplanted
tumors
using a
Maxwell RSC simplyRNA Tissue Kit
(
Promega
Corp., Fitchburg, WI, USA; cat. #
AS1340
). Using the Low Input Quick Amp
Labeling
Kit 1-color (
Agilent Technologies
, Santa Clara, CA, USA; #
5190–2305
),
(76)
Identification of germline cancer predisposition variants in pediatric sarcoma patients from somatic tumor testing
Scientific Reports
February 20, 2023
Piedad Alba-Pavón, Lide Alaña, Miriam Gutierrez-Jimeno, Susana García-Obregón, Teresa Imízcoz, Elena Panizo, Paula González-Urdiales, Aizpea Echebarria-Barona, Ricardo Lopez Almaraz, Laura Zaldumbide,
et al.
(
Promega
,
AS1610
),
Maxwell RSC RNA FFPE kit
(
Promega
,
AS1440
) and Maxwell RSC Simply RNA tissue (
Promega
,
AS1340
). DNA and RNA were quantified using a
Qubit fluorometer
and adjusted to a final quantity of 50 ng of
(77)
Integrative multi-omics networks identify PKCδ and DNA-PK as master kinases of glioblastoma subtypes and guide targeted cancer therapy
Nature Cancer
February 2, 2023
Simona Migliozzi, Young Taek Oh, Mohammad Hasanain, Luciano Garofano, Fulvio D’Angelo, Ryan D. Najac, Alberto Picca, Franck Bielle, Anna Luisa Di Stefano, Julie Lerond,
et al.
Maxwell Rapid Sample Concentrator Instrument (Promega) and
Maxwell RSC simplyRNA Tissue Kit
(
Promega
,
AS1340
) for frozen samples or
Maxwell RSC RNA FFPE kit
(
Promega
,
AS1440
) for FFPE specimens. RNA extracted
(78)
Low-Intensity Pulsed Ultrasound-Mediated Blood-Brain Barrier Opening Increases Anti-Programmed Death-Ligand 1 Delivery and Efficacy in Gl261 Mouse Model
Pharmaceutics
January 30, 2023
Ahmed MH, Hernández-Verdin I, Quissac E, Lemaire N, Guerin C, Guyonnet L, Zahr N, Mouton L, Santin M, Petiet A,
et al
.
brain tissues. mRNA was extracted using Maxwell RSC simply RNA automated
RNA purification kit
(
Promega
,
AS1340
). RNA quality was analyzed using high-
sensitivity
RNA chips. For
RNA sequencing
, NovaSeq 6000 sequencer
(79)
Cellular senescence in malignant cells promotes tumor progression in mouse and patient Glioblastoma
Nature Communications
January 27, 2023
Rana Salam, Alexa Saliou, Franck Bielle, Mathilde Bertrand, Christophe Antoniewski, Catherine Carpentier, Agusti Alentorn, Laurent Capelle, Marc Sanson, Emmanuelle Huillard,
et al.
either the TRI-reagent (Molecular Center Research, #TR 118), the Maxwell RSC simplyRNA Tissue (
Promega
, #
AS1340
), and the Macherey-Nagel Mini kit Nucleospin protocol (Macherey-Nagel, #740955.50). cDNAs were generated
(80)
Exploration of Patient-Derived Pancreatic Ductal Adenocarcinoma Ex Vivo Tissue for Treatment Response
Antioxidants (Basel)
January 11, 2023
Tímea Szekerczés, Arun Kumar Selvam, Carlos Fernández Moro, Soledad Pouso Elduayen, Joakim Dillner, Mikael Björnstedt, Mehran Ghaderi
Extraction and Two-Step
Real-Time PCR
Total-RNA was isolated using
Maxwell RSC simplyRNA Tissue Kit
(
AS1340
,
Promega Corporation
, Madison, WI, USA) by
Maxwell RSC Instrument
(
Promega Corporation
, Madison, WI,
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Maxwell RSC simplyRNA Tissue Kit from Promega
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