Maxwell RSC simplyRNA Tissue Kit AS1340 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Tissue Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1340
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeTissue samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Quantification of transcript isoforms at the single-cell level using SCALPELNat CommunJuly 11, 2025Franz Ake, Marcel Schilling, Sandra M. Fernández-Moya, Akshay Jaya Ganesh, Ana Gutiérrez-Franco, Lei Li, Mireya Plass1 µg of total mRNA extracted using Maxwell® RSC simplyRNA Cells kit protocol (Promega Corporation, #AS1340) was used as input RNA for the cDNA synthesis using an oligo(dT)-adapter sequence TAP-VN as a primer

(22) Cracking rare disorders: a new minimally invasive RNA-seq protocolNPJ Genom MedMay 28, 2025Laurenz De Cock, Erika D'haenens, Lies Vantomme, Lynn Backers, Aude Beyens, Kathleen Bm Claes, Griet De Clercq, Robin de Putter, Candy Kumps, Nika Schuermans, et al.different extraction kit was used: (1) PBMCs, fibroblasts, LCLs: Maxwell® RSC simply RNA Tissue kit (AS1340, Promega); or (2) Whole blood (from PAXgene® blood RNA tubes): Maxwell® RSC Blood simplyRNA kit (AS1380,

(23) Loss of proton-sensing GPR4 reduces tumor progression in mouse models of colon cancerMolecular oncologyMay 21, 2025Perren L, Busch M, Ruiz PA, Malagola E, Baumeler V, Foti F, Gross A, Grütter T, Edel H, Schuler C, et al.isolated from colon and ileum using the Maxwell RSC simplyRNA tissue kit (Promega, Madison, WI, USA, AS1340) as previously described in [41]. For all samples, lysis buffer from the kit was added to snap‐frozen

(24) Nicotine promotes AngII-induced abdominal aortic aortopathies in female and male mice: role of sex hormonesClinical ScienceApril 25, 2025Mark Ensor, Sean E. Thatcher, Kristen McQuerry, Kory Heier, Heba M. Ali, Victoria English, Lisa A. Cassis, Yasir Alsirajcells following the procedure for the Promega Maxwell RSC system using the simply RNA Tissue Kit (REF# AS1340, Promega, Madison, WI), and RNA concentration was quantified on a Nanodrop 2000. cDNA was synthesized

(25) Utility of tumor and non-tumor biopsies during percutaneous radiofrequency ablation for hepatocellular carcinomaJHEP ReportsApril 22, 2025Lorraine Blaise, Marianne Ziol, Claudia Campani, Nathalie Ganne-Carrie, Pierre Nahon, Gisele Nkontchou, Jessica Zucman-Rossi, Lucie Del Pozo, Nathalie Barget, Carina Boros, et al.USA), and RNA from frozen surgical samples was extracted using the Maxwell® RSC simplyRNA Tissue Kit (#AS1340, Promega). DNA and RNA from frozen biopsies were extracted using the AllPrep DNA/RNA/miRNA Universal

(26) Heterogeneous and novel transcript expression in single cells of patient-derived clear cell renal cell carcinoma organoidsGenome ResearchApril 14, 2025Tülay Karakulak, Natalia Zajac, Hella Anna Bolck, Anna Bratus-Neuenschwander, Qin Zhang, Weihong Qi, Debleena Basu, Tamara Carrasco Oltra, Hubert Rehrauer, Christian von Mering, et al.frozen tissue samples of ccRCC2, ccRCC3, ccRCC4, and ccRCC5 using Maxwell RSC simplyRNA Tissue (Promega AS1340). Following the manufacturer's protocol, 500 ng RNA was used to synthesize cDNA by qScript cDNA Synthesis

(27) Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism modelsElifeApril 3, 2025Ulrike Pech, Jasper Janssens, Nils Schoovaerts, Sabine Kuenen, Carles Calatayud Calatayud Aristoy, Sandra F Gallego, Samira Makhzami, Gert J Hulselmans, Suresh Poovathingal, Kristofer Davie, et al.Biolabs, Inc (NEB)",NEB #E2621, "Commercial assay, kit",Maxwell RSC instrument and kit,Promega,"AS8500, AS1340", "Commercial assay, kit",Chromium Single Cell 3′ Library & Gel Bead Kit v2,10x Genomics,PN-120237, "Commercial

(28) Differential Roles of Macrophages and Microglia in Subretinal Fibrosis Secondary to Neovascular Age-Related Macular DegenerationInvestigative ophthalmology & visual scienceMarch 3, 2025Szczepan M, Llorián-Salvador M, Yi C, Hughes D, Mack M, Chen M, Xu H.-Quantitative PCR Total RNA was extracted from RPE/choroid/sclera and retina using the Maxwell RSC simplyRNA Tissue Kit (AS1340; Promega, Madison, WI, USA). Four RPE/choroidal samples were pooled for RNA extraction and quantitative PCR

(29) EGR1 regulates oxidative stress and aldosterone production in adrenal cells and aldosterone-producing adenomasRedox BiologyMarch 1, 2025Yingxian Pang, Siyuan Gong, Martina Tetti, Zhuolun Sun, Sanas Mir-Bashiri, Martin Bidlingmaier, Thomas Knösel, Eckhard Wolf, Martin Reincke, Elisabeth Kemter, et al.Real-time qPCR analysis Total RNA was extracted using the Maxwell RSC simplyRNA tissue kit (Promega, AS1340). Reverse transcription was performed on 500 ng of RNA (GoScript™ reverse transcriptase kit, Promega,

(30) The Simultaneous Deletion of pH-Sensing Receptors GPR4 and OGR1 (GPR68) Ameliorates Colitis with Additive Effects on Multiple Parameters of InflammationInt J Mol SciFebruary 12, 2025Federica Foti, Cordelia Schuler, Pedro A. Ruiz, Leonie Perren, Ermanno Malagola, Cheryl de Vallière, Klaus Seuwen, Martin Hausmann, Gerhard Roglerisolated from the colon and ileum using the Maxwell RSC simplyRNA tissue kit (Promega, Madison, WI, USA, AS1340). For all samples, lysis buffer from the kit was added to snap frozen resections, and samples were shredded

(31) Serotonin 3 receptor antagonism reduces angiotensin II–induced abdominal aortic aneurysms: Contribution of periaortic fat–derived serotoninThe Journal of Pharmacology and Experimental TherapeuticsFebruary 1, 2025Yasir AlSiraj, Charles M. Ensor, Victoria English, Analia S. Loria, Heba Ali, Lisa A. CassisC57BL/6 mice. RNA was extracted using a Maxwell Rapid Sample Concentrator simply RNA Tissue kit (REF#AS1340, Promega). RNA was reverse transcribed to cDNA using qScript Ultra SuperMix (catalog number 95048-500,

(32) Machine learning-optimized targeted detection of alternative splicingNucleic Acids ResearchJanuary 24, 2025Kevin Yang, Nathaniel Islas, San Jewell, Di Wu, Anupama Jha, Caleb M Radens, Jeffrey A Pleiss, Kristen W Lynch, Yoseph Barash, Peter S Choipurified from cell pellets with the Maxwell RSC 48 instrument using the Maxwell RSC simplyRNA Cells Kit (#AS1340, Promega). Poly-A selection was subsequently performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module

(33) Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viabilityJournal of Cell BiologyJanuary 6, 2025Ken Ishikawa, Saeko Soejima, Takashi Nishimura, Shigeaki Saitohusing a nucleic acid purification device, Maxwell RSC (Promega Corp.), with the simplyRNA Tissue Kit (AS1340; Promega Corp.) according to manufacturer instructions with modifications, as follows. Frozen pellets

(34) Renal Proximal Tubule Cell-Specific Megalin Deletion Does Not Affect Atherosclerosis But Induces Tubulointerstitial Nephritis in Mice Fed a Western DietArteriosclerosis, thrombosis, and vascular biologyJanuary 1, 2025Amioka N, Franklin MK, Kukida M, Zhu L, Moorleghen JJ, Howatt DA, Katsumata Y, Mullick AE, Yanagita M, Martinez-Irizarry MM, et al.quantitative PCR (qPCR) Total RNA was extracted from kidneys using Maxwell® RSC simplyRNA Tissue Kits (Cat # AS1340; Promega) in accordance with the manufacturer’s protocol. Total RNA was transcribed reversely to cDNA

(35) Renal Proximal Tubule Cell-Specific Megalin Deletion Does Not Affect Atherosclerosis But Induces Tubulointerstitial Nephritis in Mice Fed a Western DietArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 1, 2025Naofumi Amioka, Michael K. Franklin, Masayoshi Kukida, Liyuan Zhu, Jessica J. Moorleghen, Deborah A. Howatt, Yuriko Katsumata, Adam E. Mullick, Motoko Yanagita, Michelle M. Martinez-Irizarry, et al.quantitative PCR (qPCR) Total RNA was extracted from kidneys using Maxwell® RSC simplyRNA Tissue Kits (Cat # AS1340; Promega) in accordance with the manufacturer’s protocol. Total RNA was transcribed reversely to cDNA

(36) Metabolomic Response to Non-Steroidal Anti-Inflammatory DrugsbioRxivDecember 5, 2024Soumita Ghosh, Nick Lahens, Kayla Barekat, Soon-Yew Tang, Katherine N. Theken, Emanuela Ricciotti, Arjun Sengupta, Robin Joshi, Frederic D. Bushman, Aalim Weljie, et al.injection of PBS for 1 week. RNA-Seq Quantification The RNA was extracted from heart using a Promega kit (AS1340). RNA samples were quantified with NanoDrop 8000 Spectrophotometer and Agilent 21200 Bioanalyzer systems.

(37) Transcriptomic analysis of BM-MSCs identified EGR1 as a transcription factor to fully exploit their therapeutic potentialBiochimica et Biophysica Acta (BBA) - Molecular Cell ResearchDecember 1, 2024Ludovica Santi, Stefano Beretta, Margherita Berti, Evelyn Oliva Savoia, Laura Passerini, Marilena Mancino, Giada De Ponti, Gaia Alberti, Pamela Quaranta, Luca Basso-Ricci, et al.the manufacturer's instructions of the Maxwell® RSC simplyRNA Cells and Tissue Kits (Promega, Cat. AS1340). After extraction, RNA samples were quantified using the NanoDrop 2000 (Thermo Scientific). 30 ng of

(38) Role of long non‑coding RNA leucine‑rich repeat containing 75 A‑antisense RNA1 in the invasion and progression of renal cell carcinomaOncology ReportsNovember 14, 2024Takanori Tokunaga, Hiroshi Hirata, Yukihiro Hitaka, Nakanori Fujii, Keita Kobayashi, Takahide Hayano, Yoshiyuki Asai, Koji Shiraishiextracted from each of these microdissected tissues using the Maxwell® RSC simplyRNA Tissue Kit (cat. no. AS1340; Promega Corporation). The total RNA concentration was measured using NanoDrop One (Thermo Fisher Scientific,

(39) Duchenne Muscular Dystrophy in Two Half-Brothers Due to Inherited 306 Kb Inverted Insertion of 10p15.1 into Intron 44 of the Dp427m Transcript of the DMD GeneInternational journal of molecular sciencesNovember 6, 2024Jepsen WM, Fazenbaker A, Ramsey K, Bonfitto A, Naymik M, Turner B, Sloan J, Tiwari N, Bernes SM, Neilson DE, et al.that remained after the original splicing analyses were performed. RNA was extracted using the Maxwell AS1340 RSC Simply RNA Tissue kit (Promega, Fitchburg, WI, USA) and the Quick-RNA Miniprep Plus kit (Zymo Research,

(40) Effects of brain microRNAs in cognitive trajectory and Alzheimer’s diseaseActa NeuropathologicaOctober 30, 2024Selina M. Vattathil, Sarah Sze Min Tan, Paul J. Kim, David A. Bennett, Julie A. Schneider, Aliza P. Wingo, Thomas S. Wingoextracted from dlPFC tissue samples using the Maxwell RSC simplyRNA Tissue Kit (Promega, catalog number AS1340). In detail, frozen brain tissue samples were dissected to 30 mg each. Each tissue sample was expeditiously

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Maxwell RSC simplyRNA Tissue Kit

Maxwell RSC simplyRNA Tissue Kit from Promega

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Maxwell RSC simplyRNA Tissue Kit from Promega

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