CellTiter-Glo 2.0 Assay from Promega

Product Specs
ItemCellTiter-Glo 2.0 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG9242
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathwaysRedox ReportDecember 31, 2026Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan HuangMadison, WI, USA), whereas cell viability was measured using the CellTiter-Glo® 2.0 Assay (catalog no.: G9241; Promega). Fluorescence or luminescence signals were detected using a Varioskan LUX plate reader (Thermo Fisher Scientific

(2) Apolipoprotein E lipidation, rather than isoform identity, determines amyloid-β binding and astrocyte clearanceJournal of Biological ChemistryNovember 1, 2026Kamila Nurmakova, Gregory-Neal W. Gomes, Zachary A. Levinewas applied to each well for 24 h. After incubation, 100 μl of Cell Titer Glo 2.0 reagent (Promega, G9242) was added to each well and incubated following the manufacturer’s recommendations. The luminescence

(3) 3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamicsBioactive MaterialsNovember 1, 2026Zhijie Yang, Jing Na, Yu Liu, Gan Yue, Fei Song, Chiyu Li, Qiusheng Shi, Yubo Fan, Lisha Zhengcells per 100 μl. 100 μl cell suspension and 100 μl CellTiter-Glo® 2.0 Cell Viability Assay reagent (G9241, Promega, WI, USA) were mixed rapidly in opaque 96-plates and the luminescent output was detected by

(4) PDS5A and TOP2B cooperate for chromatin recruitment via CTCFSci AdvOctober 30, 2026Edgar Gonzalez-Buendia, Havva Ortabozkoyun, Danny Reinbergand 7.5 μM) were added to the medium. After 0, 24, 48, and 72 hours of treatment, CellTiter-Glo 2.0 (G9242, Promega) luminescent reagent was used for lysis and detection of viable cells. Four technical replicates

(5) Curcumin-loaded alkali-heat-treated titanium modulates macrophage inflammatory responsesBiomater SciOctober 29, 2026Andrew Cedillo, Niloufar Abedi, Sahar Vahabzadeh, Miya Kanggroup). Cell viability was assessed using the CellTiter-Glo® 2.0 Luminescent Cell Viability Assay (G9241, Promega), which measures intracellular ATP levels as an indicator of metabolically active cells via

(6) Modeling microbiome modulation of tumor metabolic networks to predict synergistic therapiesCell Reports MethodsOctober 19, 2026Annie J. Badenoch, Zeyang Pang, Carolina H. Chung, Aaron Robida, Bretton Badenoch, Ritish Natesan, Layth Kakish, Jiahe Li, Sriram Chandrasekaranthe cell monolayer viability For cell viability verification, the CellTiter-Glo® 2.0 assay (Promega, G9241) was used. After co-culture, the cell monolayer was removed, and both the apical and basolateral chamber

(7) Benzoylaconine suppresses colorectal cancer progression by modulating the CDK2/MMP2 signaling axisFront PharmacolOctober 16, 2026Fengyi Li, Ke Zhang, Miao Wang, Liya Su, Ling Yangdissociation solution (Cat. No. NBS5322) were purchased from Nanjing Nuodian Biotech. CellTiter-Glo® 2.0 (Cat. G9242) was sourced from Promega (United States). 2.2 Cell culture The human colorectal cancer cell lines

(8) Mixture-Dependent Toxicity of E-Cigarette Constituents: Cytotoxicity, Inflammation, and DNA-Repair-Associated Protein Responses in Bronchial Epithelial CellsToxicsOctober 15, 2026Ehsanul Kabir, Joshua Furst, Thivanka MuthumalageATP was measured 24 h after treatment using CellTiter-Glo 2.0 (Promega, Madison, WI, USA, Cat. No. G9241). ATP standards were 0, 1 nM, 10 nM, 100 nM, 1 µM, 10 µM, and 100 µM. CellTiter-Glo reagent (100 µL)

(9) Multi-omics profiling identifies triciribine as a biomarker-guided antimetabolite therapy for acute lymphoblastic leukemiaNPJ Precis OncolOctober 14, 2026Xuekang Qi, Georgios Mermelekas, Ondrej Hodek, Luay Aswad, Quentin Glaziou, Isabelle Rose Leo, Nona Struyf, Femke M. Hormann, Ioannis Siavelis, Henri Colyn Bwanika, et al.TCN for 72 h, after which cell viability was measured using CellTiter-Glo 2.0 ATP reagent (Promega; G9242) on a Victor 3 V plate reader. Relative cell viability values were subsequently curve-fitted using a

(10) Inhibition of BAP1 Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Promoting BIRC5 DegradationPharmaceuticals (Basel)October 8, 2026In-Ho Jeong, Yu-Jin Park, Min-Jee Kim, Eun Ju Lee, Byung Sik Kim, Hee-Sung Kim, Peter Chang-Whan Leeperformed using the CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, Madison, WI, USA, G9241). Cells were seeded into a 96-well white plate at a concentration of 1 × 103 cells/100 µL and cultured

(11) Targeting the Cav1.2-KCa1.1 axis prevents rabies virus internalization and protects against lethal infectionPLoS PathogOctober 6, 2026Ziruo Sun, Jinqiu Wang, Zhiyuan Wen, Lei Shuai, Wenjing Sun, Mengjie Yang, Jinyu Wang, Junyu Chen, Tengteng Mi, Xianjing Zhou, et al.Anti-Flag-tag antibody-conjugated agarose beads (CN: A2220) were from Sigma-Aldrich. CellTiter-Glo kit (CN: G9242) was from Promega. Diltiazem (CN: D2521-1G) was from Sigma-Aldrich. ExFect Transfection Reagent (CN:

(12) Aberrant Accumulation of Cyclin D1 Rewires Glutamine Metabolism and Generates a Metabolic Vulnerability in Squamous Cell CarcinomaCancer ResOctober 5, 2026Shuyuan Zhang, Polly J. Phillips-Mason, Bartosz Mucha, Marganit Farago, Guofang Zhang, Cecilia Martin, Andres J. Klein-Szanto, Hiroshi Nakagawa, Anil K. Rustgi, Kwok-Kin Wong, et al.protocol. Cellular ATP levels For cellular ATP levels analysis, CellTiter-Glo Cell Viability Assay (Promega G9241, RRID: SCR_018197) was used and performed according to the manufacturer's instructions. The assay uses

(13) KSHV-encoded vIRF3 cooperates with cellular IRF4 to drive super-enhancer activity through complex DNA elementsNucleic Acids ResearchOctober 5, 2026Ziyan Liang, Christine B Magdongon, Haocong Katherine Ma, Maureen E Haynes, Jingyi Lu, Elizabeth T Bartom, Eva Gottweindoxycycline, and we assessed viability using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, #G9241), as instructed. We processed cells for ChIP-qPCR (chromatin immunoprecipitation coupled with qPCR)

(14) Multiomic Profiling Links RDH12-Dependent Retinaldehyde Detoxification to Membrane Remodeling and FerroptosisbioRxivOctober 1, 2026KanagaVijayan Dhanabalan, Miranda R. Muhoberac, Ramya Visvanathan, Devender Arora, Olga Belyaeva, Natalia Y. Kedishvili, Ramaswamy Subramanian, Bryon S. DrownAssays: Cell viability was evaluated using the CellTiter-Glo Luminescent Cell Viability Assay (Promega – G9241). Cells stably expressing RDH12-GFP or a GFP control were seeded into 96-well plates at a density of

(15) A critical role for mitochondrial dynamics in cigarette smoke condensate-induced RPE senescenceFree Radical Biology and MedicineOctober 1, 2026Qi Zhou, Zunyi Zhang, Yinga Wu, Chunmin Dong, Chen-Chuan Wu, James T. Handa, Jing Ma, Shusheng WangMitoSOX loading. Cellular ATP level was measured as described in the CellTiter-Glo 2.0 Assay kit (Promega G9242). Briefly, ARPE-19 cells were seeded using opaque-walled 96-well plates with 100 μl of culture medium,

(16) Adipocytes Protect Ovarian Cancer Cells from FerroptosisBiomoleculesSeptember 30, 2026Lia Tesfay, Chaeyeon Yoo, Timothy E. Moore, Jingyun Lee, Kirtikar Shukla, Cristina M. Furdui, Poornima Hegde, Evan R. Jellison, Suzy V. Tortiadipocytes and incubated for 72 h. Cell viability was analyzed using ATP-based Celltiter Glo (Promega, G9241) 24 h after RSL3 treatment. 2.8. SiRNA Knockdown A total of 12 ng of Silencer Select Human CD36 siRNA

(17) Targeting GPX4 and FSP1 to exploit ferroptotic vulnerability in Ewing sarcomaFrontiers in OncologySeptember 28, 2026Mian T. Mhindu, Stacia L. Koppenhafer, Mason G. Lyons, Jessica A.O. Zimmerman, David J. Gordonassays Cell viability was assessed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, G9241) as previously described (30, 34). Briefly, 5,000–10,000 cells were seeded per well in 96-well plates

(18) An Injectable, Self-Expanding Hemostatic Foam for Field-Compatible Postpartum Hemorrhage ManagementAdv Sci (Weinh)September 27, 2026Charlotte Meyer, Noël Strasser, Ilakkiya Ravindrarajah, Elsa Wrenger, Kristian Ivkovic, Jan Ševčík, Sima Šarčević, Lenka Červenková, Jáchym Rosendorf, Václav Liška, et al.24 h. Cell viability was assessed through the ATP dependent CellTiter‐Glo 2.0 Cell Viability Assay (#G9241, Promega, Dübendorf, Switzerland) and cytotoxicity was measured using the LDH dependent CytoTox 96 Cytotoxicity

(19) RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulationSci AdvSeptember 25, 2026Pushpamali De Silva, Samantha Fitzgerald, Jules Cazaubiel, Kristin Qian, Matan Hofree, Jia-Ren Lin, Roxanne Pelletier, Mariana Lopez Leon, Phuong Vu Mai Ho, Yasutoshi Takashima, et al.Cell viability was determined using the CellTiter-Glo 2.0 Cell Viability Assay (Promega, catalog no. G9242) for 2D cultures and the CellTiter-Glo 3D Cell Viability Assay (Promega, catalog no. G9681) for 3D cultures,

(20) HBV Infection Induces Changes in Mitochondrial Architecture and Remodeling of Mitochondria–ER InteractionsPathogensSeptember 24, 2026Leonardo Duca, Carlotta Castelli, Anna Rosa Garbuglia, Laura FalascaMeasurement ATP levels were measured using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Cat. No. G9241, Promega Corporation, Madison, WI, USA). Cells (uninfected and infected cells at 2, 5, and 10 dpi) seeded

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(1)

9/21/2026Fast Cell Viability Assay Shivali RawatCellTiter-Glo 2.0 provides a simple luminescence-based readout of ATP and is convenient for multiwell experiments
(1)

9/4/2026Cell Viability Assay Using CTG 2.0 Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway drives cancers of the stomach, intestine, and breast, among others. I am interested in... Read Review