Promega
CellTiter-Glo® 2.0 Cell Viability Assay
G9242
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway drives cancers of the stomach, intestine, and breast, among others. I am interested in studying the molecular mechanisms of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention in colon cancer. The goal of this experiment is to optimize cell seeding density in order to maximize the signal-to-noise ratio of the CellTiter-Glo 2.0 cell viability assay.
Cell Culture
LoVo cell line
Cell seeding density needs to be optimized
Cell line: LoVo, a human colorectal cancer line carrying a truncating mutation in APC. Seeding format: 96-well plate, 200 µL medium per well, at 1,000 or 2,000 cells per well. Treatment: 20–24 h after seeding, cells were treated with a cytotoxic compound at a concentration known to reduce viability in this line. Readout: 120 h after treatment, viability was measured using CellTiter-Glo 2.0 according to the manufacturer's instructions for reagent equilibration, sample preparation, and luminescence detection.
Ease of use and product performance
None
The product is on the expensive side, but Promega's products and technical literature are top-notch.