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CellTiter-Glo 2.0 Assay
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CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay from Promega
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Citations:
(985)
Figures:
(4)
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CellTiter-Glo 2.0 Assay
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Description
The CellTiter-Glo 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo 2.0 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G9242
Quantity
100ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (985)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(catalog no.:
G9241
;
Promega
).
Fluorescence
or
luminescence
signals were detected using a Varioskan LUX plate reader (
Thermo Fisher Scientific
(2)
Investigating the role of mimosine-induced genotoxic stress through DNA repair profiling
Mol Ther Nucleic Acids
June 16, 2026
Sai Pallavi Pradeep, Suraj Kalaria, Peter M. Glazer, Raman Bahal
0.125, 0.25, 0.5, and 1 mM). After 24 h, cell viability was measured using CellTiter-Glo (
Promega
, #
G9241
), and
luminescence
was recorded using TECAN infinite 200 PRO M-plex microplate reader. Statistical
(3)
Preclinical Characterization and Clinical Activity of RNK08954, a Highly Selective and Orally Bioavailable KRAS G12D Inhibitor
Cancer Discovery
May 1, 2026
Ling Xie, Chunwei Xu, Han Si, Xiaoshuang Fu, Xiangcai Yang, Jianan Jin, Xiaoqing Yu, Zhiying Shao, Yanling Wang, Tianqing Chu,
et al.
purchased from
Bio X Cell
.
In Vitro
Assays
Cell Proliferation Inhibition Assay (CTG 2.0
Assay
,
Promega
,
G9243
) In the cell growth inhibition experiments, each tested compound was serially diluted in threefold,
(4)
Non-destructive transcriptomics via vesicular export
Nat Commun
April 25, 2026
Niklas Armbrust, Martin Grosshauser, Julian Geilenkeuser, Luisa Stroppel, Mattea Jozinovic, Hella Levermann, Tobias Panne, Jannick Wißmann, Lukas Goelitz, Sebastian Schmidt,
et al.
was induced with different dox concentrations. The CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
,
G9241
), as well as
LDH-Glo Cytotoxicity Assay
(
Promega
,
J2380
), was performed in triplicate according to the
(5)
A degrader of HER2 and EGFR abolishes p95HER2 and shows robust antitumor efficacy in HER2-positive breast cancer
Sci Rep
April 20, 2026
Lu Yang, Arup Bhattacharya, Yun Li, Darrell Peterson, Yuesheng Zhang
Reader from
BioTek
. UACC-812 cell growth was measured using the CellTiter-Glo
Assay
from
Promega
(Cat #
G9241
) in 96-well plates. Notably, UACC-812 cells grew relatively slowly, and the
MTT assay
produced low signal
(6)
Inhibitory role of angiopoietin‑like 4 for cancer progression in oropharyngeal squamous cell carcinoma
Oncol Rep
April 20, 2026
Takuya Mikoshiba, Mariko Sekimizu, Nana Nakahara, Shin Saito, Keisuke Yoshihama, Ryoto Nagai, Takeyuki Kono, Hiroyuki Ozawa
viability was subsequently assessed using a CellTiter-Glo 2.0
luminescence
-based
assay
kit (cat. no.
G9241
;
Promega Corporation
). The results were normalized to those of the negative control (set as 1.0).
Immunofluorescence
(7)
Discovery of Autophagy Modulators that Increase I1061T NPC1 Expression and Promote Cholesterol Efflux in Niemann-Pick Type C Patient-Derived Fibroblasts
ACS Chem Biol
April 17, 2026
Maryna Salkovski, Andrea Arrieche Suarez, Qiwen Gao, Ryan S. Hippman, Zoe A. Petros, Melissa A. Korkmaz-Vaisys, Erica M. Gerlach, Yaneris M. Alvarado-Cartagena, Thu T. A. Nguyen, Ivan Pavlinov,
et al.
in 37 °C and 5% CO2 for 24 h. The next day, the
assay
plate and CellTiter-Glo 2.0 reagent (
Promega
,
G9242
) were both incubated at rt for 30 min, and then 25 μL of CellTiter-Glo 2.0 reagent was added to each
(8)
A multimodal atlas for immunotherapeutic targeting of AML surface heterogeneity
iScience
April 17, 2026
Matthew Ung, Julia Etchin, Amanda Halfond, Julia DiFazio, Yonina Keschner, Alyssa Pyclik, Anne Campbell, Ruijia Wang, Mariana Silva, Brikena Gjeci,
et al.
buffer,"
Rockland Immunochemicals, Inc
.",Cat#
MB-089-0500
CellTiter-Glo® 2.0
Cell Viability Assay
,
Promega
,Cat#
G9241
CellTrace™
Violet
,ThermoFisher Invitrogen™,Cat#
C34571
CFSE,
ThermoFisher
Invitrogen™,Cat# C34570 SpCas9,Aldevron
(9)
MRE11 proximal polyadenylation site-mediated looping impacts transcription and genomic stability
Mol Cell
April 16, 2026
Kaimeng Huang, Marie Eve Brault, Ke Cong, Narmen Azazmeh, Sarah Collins, Siyun Lee, George A. Lantz, Gregory Raskind, Gaëlle Legube, Rameen Beroukhim,
et al.
assays
,Critical commercial
assays
,Critical commercial
assays
CellTiter-Glo 2.0
Cell Viability Assay
,
Promega
,Cat#
G9242
Click-iT EdU Alex Fluor 488 Kit,
Invitrogen
,Cat#
C10633
"Click-iT EdU Cell Proliferation Kit for Imaging,
(10)
Synthetic lethality of decitabine plus ATR inhibition for TP53-mutated AML∗
Blood Advances
April 14, 2026
Jeremy T. Baeten, Sumedha Agashe, Imene Tabet, Jack T. Wooldridge, Amber Carter, Jamie N. Butler, Christopher A. Miller, Nichole M Helton, Annabel Quinet, Kimberly B. Johansson,
et al.
assays
AML cell lines and primary patient cells were subjected to the CellTiter-Glo (CTG) 2.0
assay
(#
G9241
;
Promega
) and the
Caspase-Glo 3/7 assay
(#
G8091
;
Promega
) as described previously.29 Briefly, cells
(11)
Evodiamine Regulates Oxidative Stress and the JAK2/STAT3 Pathway to Modulate Apoptosis, Inflammation, Cell Cycle Arrest, and Migration in Periodontal Ligament Cells
Antioxidants (Basel)
April 10, 2026
Chuan Wang, Yuting Wen, Peiren Xu, Dong Yang
percentage relative to the untreated control. The
CellTiter-Glo® Luminescent Cell Viability Assay
kit (
G9241
,
Promega
, Madison, WI, USA) was used to quantify ATP levels for determining cellular metabolic activity,
(12)
The c-Abl-RIPK3 Axis Drives Mitochondrial Dysfunction and Impaired Mitophagy in Gaucher Disease Models
Antioxidants (Basel)
April 9, 2026
Cristian M. Lamaizon, Renatta Tironi-Hernández, Nohela B. Arévalo, Sebastián D. Ahumada, Daniela A. Gutiérrez, Laura Brito-Fernández, Andrea del Campo, Silvana Zanlungo, Alejandra R. Álvarez
were determined using the CellTiter-Glo® 2.0 Cell Viability Assay kit (
Promega
, Madison, WI, USA, #
G9241
), and
luminescence
was measured using the Cytation 5 instrument (BioTek Instruments, Winooski, VT, USA).
(13)
Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells
bioRxiv
April 8, 2026
Shiyuan Wang, Liam Yourston, Gembu Maryu, Yeonghoon Kim, Derek Walker, Usha Kadiyala, Qiong Yang
Luciferase reagents CellTiter-Glo 2.0 were used to estimate ATP levels in the extract solution (
Promega
,
G9241
). A standard curve was prepared with ATP water solutions prepared between 0.1 μM to 10 μM. ATP solutions
(14)
PDS5A and TOP2B cooperate for chromatin recruitment via CTCF
bioRxiv
April 3, 2026
Edgar Gonzalez-Buendia, Havva Ortabozkoyun, Danny Reinberg
7.5 μM) were added to the media. After 0, 24, 48, and 72 hr of treatment, CellTiter-Glo 2.0 (
Promega
,
G9242
) luminescent reagent was used for lysis and detection of viable cells. Four technical replicates per
(15)
Deletion of SPI1 in microglia exacerbates amyloid pathology by impairing microglial response in Alzheimer's disease models
Neuron
April 1, 2026
Byungwook Kim, Mason Douglas Tate, Hande Karahan, H.R. Sagara Wijeratne, Ahmad Daniel Sharify, Selena S. Wang, Justin R. Kim, Md Mamun Al-Amin, Kelly Hartigan, Sunwoo Chung,
et al.
SYBR Green PCR Master Mix
,
Applied Biosystems
,Cat# 43-856-14 CellTiter-Glo® 2.0
cell viability assay
,
Promega
,Cat#
G9242
Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
MSD
V-PLEX Aβ Peptide Panel 1 (6E10) Kit
(16)
Comparative effectiveness of seven major human let-7-5p isoforms to modulate target gene expression in liver cells
Drug Metabolism and Disposition
April 1, 2026
Joseph M. Cronin, Mei-Juan Tu, Yimei Wang, Ai-Ming Yu
R2061) was purchased from
Zymo Research
(Irvine, CA). CellTiter-Glo 2.0 Cell Viability Assay kit (Cat#
G9241
) was purchased from
Promega
(Madison, WI). Several isoform-specific
stem
-loop RT and
qPCR
primers were
(17)
Efficacy of an XPO1 inhibitor in combination with irinotecan in a preclinical colorectal cancer model
Frontiers in Oncology
March 31, 2026
Robert W. Lentz, Adrian T. A. Dominguez, Nicole Baranda Balmaceda, John J. Arcaroli, Stacey M. Bagby, Cameron A. Binns, Stephen G. Smoots, Marilyn M. Jackson, Morgan Macbeth, Tessa Holmstoen,
et al.
additional 48 hours. Cells were then treated with CellTiter-Glo 2.0
Cell Viability Assay
reagent (
Promega
#
G9243
), and viability was measured by
luminescence
. Results are the mean of triplicate measurements.
Immunoblotting
Proteins
(18)
Comprehensive analysis of TEAD inhibition in meningioma identifies MEK and mTOR inhibition as effective combination therapies against resistant lines
bioRxiv
March 30, 2026
Dylan J Keiser, Meghan S Buddy, Solmaz Mojarad-Jabali, Qing Li, Missia Kohler-Skinner, Abigail G Parrish, David Gillespie, David Nix, Sheri Holmen, Howard Colman,
et al.
2 hours. Endpoint viability was measured using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
G9241
) according to the manufacturer’s instructions.
Luminescence
was detected using a Microplate Luminometer.
(19)
Alternative splicing in pediatric central nervous system tumors highlights oncofetal candidate CLK1 exon 4
Neuro-Oncology Pediatrics
March 30, 2026
Ammar S Naqvi, Patricia J Sullivan, Ryan J Corbett, Priyanka Sehgal, Karina L Conkrite, Komal S Rathi, Brian M Ennis, Katharina E Hayer, Bo Zhang, Miguel A Brown,
et al.
At the end point of the
assay
, cell viability was analyzed with CellTiter Glo 2.0 reagent (
Promega
,
G9242
) by replacing half the media with reagent and reading on a Promega GloMax device. Cirtuvivint (MedChem
(20)
Gigobolins A–C, New Ophiobolins with Anticancer Activity from the Phytopathogenic Fungus Drechslera gigantea
Journal of Natural Products
March 27, 2026
Marianna Carbone, Sridharan Jayamohan, Amelia Scott, Angela Boari, Ryan J. Rutledge, Nadia Cacciapuoti, Vamika Gautam, Jaquelin Aroujo, Tania Betancourt, Daniel Romo,
et al.
on GSC viability was assessed using the CellTiter-Glo 2.0
Cell viability assay
(
Promega
catalog no.
G9241
, Madison, WI) following the manufacturer’s protocol. GSCs (4 × 103 cells) were treated for 7 days with
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CellTiter-Glo 2.0 Assay from Promega
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