Maxwell RSC simplyRNA Cells Kit AS1390 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Cells Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1390
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFresh or frozen cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) A dopamine D1-like receptor-specific agonist improves the survival of septic miceiScienceApril 19, 2024Koichi Tanaka, Mohammed E. Choudhury, Satoshi Kikuchi, Ikuko Takeda, Kensuke Umakoshi, Noriyuki Miyaue, Kanta Mikami, Ayane Takenaga, Harumichi Yagi, Rintaro Shinabe, et al.,Miltenyi Biotec,Catalog number: 130-095-927 Maxwell® RSC simplyRNA Cells / Tissue Kit,Promega,Catalog number: AS1390 RNeasy micro kit,Qiagen,Catalog number: 74004 qPCR RT Master Mix with gDNA Remover,Toyobo,Code number:

(62) Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA moleculesRNAApril 1, 2024Katherine M. McKenney, Robert P. Connacher, Elise B. Dunshee, Aaron C. Goldstrohm500g for 5 min. RNA was purified from the cell pellet using the Maxwell simplyRNA Cells Kit (Promega, AS1390) and Maxwell RSC instrument, following the manufacturer's protocol. For the on-bead DNase digestion,

(63) Oligo-PROTAC strategy for cell-selective and targeted degradation of activated STAT3Molecular Therapy - Nucleic AcidsMarch 12, 2024Jeremy Hall, Zhuoran Zhang, Supriyo Bhattacharya, Dongfang Wang, Marice Alcantara, Yong Liang, Piotr Swiderski, Stephen Forman, Larry Kwak, Nagarajan Vaidehi, et al.protein assays For qPCR, total RNA was extracted from cultured cells using the Maxwell RSC simplyRNA Cells system (AS1390; Promega, Madison, WI), then reverse transcribed into cDNAs with the iScript cDNA synthesis kit (Bio-Rad).

(64) Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic StudyBiomedicinesJanuary 15, 2024Osvaldo Artimagnella, Emanuela Mazzon, Stefano Salamone, Federica Pollastro, Agnese Gugliandolo, Luigi Chiricostaprocessed for RNA extraction. Total RNA was extracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument. Library preparation of two biological

(65) A new physiological medium uncovers biochemical and cellular alterations in Lesch-Nyhan disease fibroblastsMolecular MedicineJanuary 3, 2024Paula Escudero-Ferruz, Neus Ontiveros, Claudia Cano-Estrada, Diane J. Sutcliffe, H. A. Jinnah, Rosa J. Torres, José M. LópezLND fibroblasts cultivated with different media using the Maxwell RSC simply RNA Cells Kit (Promega, AS1390) following the manufacturer’s instructions. This procedure includes DNase I treatment to prevent DNA contamination

(66) Co-targeting HSP90 alpha and CDK7 overcomes resistance against HSP90 inhibitors in BCR-ABL1+ leukemia cellsCell death & diseaseDecember 6, 2023Vogt M, Dienstbier N, Schliehe-Diecks J, Scharov K, Tu JW, Gebing P, Hogenkamp J, Bilen BS, Furlan S, Picard D, et al.Briefly, RNA was isolated utilizing the Maxwell® RSC simplyRNA cells kit (Promega, Madison, WI, USA, #AS1390). Library preparation was carried out following supplier’s guidelines, using the VAHTs Stranded mRNA-Seq

(67) Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin SignalingbioRxivNovember 23, 2023Seong-Yeon Bae, Yi Chen, Hong Chen, Dhiraj Kumar, Spyros Karaiskos, Jane Xu, Chao Lu, Aaron D. Viny, Filippo G. Giancottianalysis Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell, Promega). cDNAs were synthesized using High-Capacity

(68) Molecular landscape and functional characterization of centrosome amplification in ovarian cancerNature CommunicationsOctober 16, 2023Carolin M. Sauer, James A. Hall, Dominique-Laurent Couturier, Thomas Bradley, Anna M. Piskorz, Jacob Griffiths, Ashley Sawle, Matthew D. Eldridge, Philip Smith, Karen Hosking, et al.extracted from cell pellets using the Maxwell® RSC Cultured Cells DNA and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega, AS8500). DNA sequencing Tagged-Amplicon Sequencing

(69) Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo modelMolecular Therapy - Nucleic AcidsSeptember 12, 2023Kazuki Heishima, Nobuhiko Sugito, Chikara Abe, Akihiro Hirata, Hiroki Sakai, Yukihiro Akaoanalysis Total RNA was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, AS1390) and a Maxwell RSC instrument (Promega, AS4500). BC cells (253 JB-V) were transfected and incubated

(70) Efficient T cell adoptive transfer in lymphoreplete hosts mediated by transient activation of Stat5 signalingMolecular TherapySeptember 1, 2023Megan D. Tennant, Christina New, Leonardo M.R. Ferreira, Richard T. O’Neilkit. RNA was extracted with a Promega Maxwell RSC 16 using the Maxwell RSC simplyRNA Cells kit (cat# AS1390) according to the manufacturer’s instructions, and RNA concentration was measured using a NanoDrop 8000. Gene

(71) Purine Nucleotide Alterations in Tumoral Cell Lines Maintained with Physiological Levels of Folic AcidInternational Journal of Molecular SciencesAugust 8, 2023Claudia Cano-Estrada, Lidia de Benito-Gómez, Paula Escudero-Ferruz, Neus Ontiveros, Daniel Iglesias-Serret, José M. Lópezfrom the different cell lines using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, USA, AS1390) following the manufacturer’s instructions. This procedure includes DNase I treatment to prevent DNA contamination

(72) Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periodsCell Rep MethodsJune 26, 2023Otte F, Zhang Y, Spagnuolo J, Thielen A, Däumer M, Wiethe C, Stoeckle M, Kusejko K, Klein F, Metzner KJ, et al.AS1620 GoTaq® Probe qPCR Master Mix,Promega,Cat#: A6102 Maxwell® RSC simply RNA cells kit,Promega,Cat#: AS1390 qRT-PCR Brilliant III probe master mix kit,Agilent,Cat#: 600884 ImmunoCult™-XF T cell Expansion Medium,STEMCELL Technologies

(73) Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFαCell & bioscienceJune 24, 2023Oppezzo A, Monney L, Kilian H, Slimani L, Maczkowiak-Chartois F, Rosselli F.loading control. qRT-PCR Total RNA was isolated using the Maxwell® RSC simplyRNA Cells Kit (Promega, #AS1390) and reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, #K1621).

(74) Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trialCancer ScienceMay 3, 2023Kazutaka Fujita, Ryo Arai, Satoshi Shoji, Ryota Saito, Motoko Nomura, Takamasa Hotta, Hajime Asahina, Masanori Kawakami, Ichiro Nakachi, Yukihiro Hasegawa, et al.DNA was extracted using the Promega Maxwell RSC AS1400 (Promega), and RNA was extracted using the RSC AS1390. Nucleotide sequences were obtained using the MiSeq system with the MiSeq Reagent Kit version 3 (Illumina). 2.5

(75) Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agentsCellMarch 2, 2023Jidong Zhang, Christine Lair, Christine Roubert, Kwame Amaning, María Belén Barrio, Yannick Benedetti, Zhicheng Cui, Zhongliang Xing, Xiaojun Li, Scott G. Franzblau, et al.H32720 Maxwell RSC Cultured Cells DNA Kit,Promega,Cat#AS1620 Maxwell RSC simply RNA Cells kit,Promega,Cat#AS1390 SuperScript™ IV VILO,Applied Biosystems,Cat#11756500 ,, Deposited data,Deposited data,Deposited data ,, 70S

(76) Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylationNature CommunicationsJanuary 6, 2023Carla L. Esposito, Ida Autiero, Annamaria Sandomenico, H. Li, Mahmoud A. Bassal, Maria L. Ibba, Dongfang Wang, Lucrezia Rinaldi, Simone Ummarino, Giulia Gaggi, et al.extraction. Maxwell RSC tissue DNA kit (Promega, AS1610) and Maxwell RSC simply RNA cells kit (Promega, AS1390) were used. Genomic DNAs samples were analyzed by Diagenode EPIC methylation array after bisulfite conversion.

(77) Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 inductionFrontiers in immunologyJanuary 1, 2023Velnati S, Centonze S, Rossino G, Purghè B, Antona A, Racca L, Mula S, Ruffo E, Malacarne V, Malerba M, et al.to RNA extraction using RNeasy Plus mini kit from Qiagen or Maxwell RSC simply RNA extraction kit (AS1390, Promega Italia), following manufacturer instructions. The RNA concentration and purity were estimated

(78) Functional noninvasive detection of glycolytic pancreatic ductal adenocarcinomaCancer & metabolismDecember 9, 2022Heid I, Münch C, Karakaya S, Lueong SS, Winkelkotte AM, Liffers ST, Godfrey L, Cheung PFY, Savvatakis K, Topping GJ, et al.cells were collected for RNA isolation. RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (no. AS1390, Promega, Germany). Cell RNA isolation kit was used according to the manufacturer’s instructions. Total

(79) PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamusNature CommunicationsDecember 1, 2022E. R. Velasco et al.stored. Total RNA was isolated and purified from the tissue with Maxwell RSC simplyRNA Tissue Kit #AS1390 (Promega Biotech Ibérica, Spain)99. Gene expression changes were detected by relative quantitative reverse

(80) High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistanceNature communicationsSeptember 22, 2022Cortés-López M, Schulz L, Enculescu M, Paret C, Spiekermann B, Quesnel-Vallières M, Torres-Diz M, Unic S, Busch A, Orekhova A, et al.was extracted using the Maxwell RSC Instrument (Promega) and the Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega). 0.5 µg or RNA were reverse-transcribed using the SuperScript™ IV Reverse Transcriptase kit

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Maxwell RSC simplyRNA Cells Kit

Maxwell RSC simplyRNA Cells Kit from Promega

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Maxwell RSC simplyRNA Cells Kit from Promega

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