Maxwell RSC simplyRNA Cells Kit AS1390 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Cells Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1390
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFresh or frozen cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin SignalingbioRxivNovember 23, 2023Seong-Yeon Bae, Yi Chen, Hong Chen, Dhiraj Kumar, Spyros Karaiskos, Jane Xu, Chao Lu, Aaron D. Viny, Filippo G. Giancottianalysis Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell, Promega). cDNAs were synthesized using High-Capacity

(62) Molecular landscape and functional characterization of centrosome amplification in ovarian cancerNature CommunicationsOctober 16, 2023Carolin M. Sauer, James A. Hall, Dominique-Laurent Couturier, Thomas Bradley, Anna M. Piskorz, Jacob Griffiths, Ashley Sawle, Matthew D. Eldridge, Philip Smith, Karen Hosking, et al.extracted from cell pellets using the Maxwell® RSC Cultured Cells DNA and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega, AS8500). DNA sequencing Tagged-Amplicon Sequencing

(63) Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo modelMolecular Therapy - Nucleic AcidsSeptember 12, 2023Kazuki Heishima, Nobuhiko Sugito, Chikara Abe, Akihiro Hirata, Hiroki Sakai, Yukihiro Akaoanalysis Total RNA was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, AS1390) and a Maxwell RSC instrument (Promega, AS4500). BC cells (253 JB-V) were transfected and incubated

(64) Efficient T cell adoptive transfer in lymphoreplete hosts mediated by transient activation of Stat5 signalingMolecular TherapySeptember 1, 2023Megan D. Tennant, Christina New, Leonardo M.R. Ferreira, Richard T. O’Neilkit. RNA was extracted with a Promega Maxwell RSC 16 using the Maxwell RSC simplyRNA Cells kit (cat# AS1390) according to the manufacturer’s instructions, and RNA concentration was measured using a NanoDrop 8000. Gene

(65) Purine Nucleotide Alterations in Tumoral Cell Lines Maintained with Physiological Levels of Folic AcidInternational Journal of Molecular SciencesAugust 8, 2023Claudia Cano-Estrada, Lidia de Benito-Gómez, Paula Escudero-Ferruz, Neus Ontiveros, Daniel Iglesias-Serret, José M. Lópezfrom the different cell lines using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, USA, AS1390) following the manufacturer’s instructions. This procedure includes DNase I treatment to prevent DNA contamination

(66) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.WI, USA), Maxwell® RSC simplyRNA tissue kit (AS1340, Promega), or Maxwell® RSC simplyRNA cells kit (AS1390, Promega) with Maxwell® RSC Instrument (AS4500, Promega). Amounts of RNA were measured with QuantiFluor® RNA System

(67) Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periodsCell Rep MethodsJune 26, 2023Otte F, Zhang Y, Spagnuolo J, Thielen A, Däumer M, Wiethe C, Stoeckle M, Kusejko K, Klein F, Metzner KJ, et al.AS1620 GoTaq® Probe qPCR Master Mix,Promega,Cat#: A6102 Maxwell® RSC simply RNA cells kit,Promega,Cat#: AS1390 qRT-PCR Brilliant III probe master mix kit,Agilent,Cat#: 600884 ImmunoCult™-XF T cell Expansion Medium,STEMCELL Technologies

(68) Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFαCell & bioscienceJune 24, 2023Oppezzo A, Monney L, Kilian H, Slimani L, Maczkowiak-Chartois F, Rosselli F.loading control. qRT-PCR Total RNA was isolated using the Maxwell® RSC simplyRNA Cells Kit (Promega, #AS1390) and reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, #K1621).

(69) Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trialCancer ScienceMay 3, 2023Kazutaka Fujita, Ryo Arai, Satoshi Shoji, Ryota Saito, Motoko Nomura, Takamasa Hotta, Hajime Asahina, Masanori Kawakami, Ichiro Nakachi, Yukihiro Hasegawa, et al.DNA was extracted using the Promega Maxwell RSC AS1400 (Promega), and RNA was extracted using the RSC AS1390. Nucleotide sequences were obtained using the MiSeq system with the MiSeq Reagent Kit version 3 (Illumina). 2.5

(70) Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agentsCellMarch 2, 2023Jidong Zhang, Christine Lair, Christine Roubert, Kwame Amaning, María Belén Barrio, Yannick Benedetti, Zhicheng Cui, Zhongliang Xing, Xiaojun Li, Scott G. Franzblau, et al.H32720 Maxwell RSC Cultured Cells DNA Kit,Promega,Cat#AS1620 Maxwell RSC simply RNA Cells kit,Promega,Cat#AS1390 SuperScript™ IV VILO,Applied Biosystems,Cat#11756500 ,, Deposited data,Deposited data,Deposited data ,, 70S

(71) Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial-Mesenchymal Transition in Endometrial Epithelial CellsBiomedicinesJanuary 14, 2023Hashimoto Y, Tsuzuki-Nakao T, Kida N, Matsuo Y, Maruyama T, Okada H, Hirota K.HIF-2 alpha/EPAS1 Antibody,Novus Biologicals,NB100-122 LY294002,FUJIFILM,1290-4861 Maxwell® RSC simply RNA Cells Kit,Promega,AS1390 "N-Acetyl-L-cysteine, ROS inhibitor",abcam,143032 p38 MAPK(D13E1) XP® Rabbit mAb,Cell Signaling TECHNOLOGY,8690 p44/42 MAPK(Erk1/2) (137F5) Rabbit mAb

(72) Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylationNature CommunicationsJanuary 6, 2023Carla L. Esposito, Ida Autiero, Annamaria Sandomenico, H. Li, Mahmoud A. Bassal, Maria L. Ibba, Dongfang Wang, Lucrezia Rinaldi, Simone Ummarino, Giulia Gaggi, et al.extraction. Maxwell RSC tissue DNA kit (Promega, AS1610) and Maxwell RSC simply RNA cells kit (Promega, AS1390) were used. Genomic DNAs samples were analyzed by Diagenode EPIC methylation array after bisulfite conversion.

(73) Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 inductionFrontiers in immunologyJanuary 1, 2023Velnati S, Centonze S, Rossino G, Purghè B, Antona A, Racca L, Mula S, Ruffo E, Malacarne V, Malerba M, et al.to RNA extraction using RNeasy Plus mini kit from Qiagen or Maxwell RSC simply RNA extraction kit (AS1390, Promega Italia), following manufacturer instructions. The RNA concentration and purity were estimated

(74) Functional noninvasive detection of glycolytic pancreatic ductal adenocarcinomaCancer & metabolismDecember 9, 2022Heid I, Münch C, Karakaya S, Lueong SS, Winkelkotte AM, Liffers ST, Godfrey L, Cheung PFY, Savvatakis K, Topping GJ, et al.cells were collected for RNA isolation. RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (no. AS1390, Promega, Germany). Cell RNA isolation kit was used according to the manufacturer’s instructions. Total

(75) PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamusNature CommunicationsDecember 1, 2022E. R. Velasco et al.stored. Total RNA was isolated and purified from the tissue with Maxwell RSC simplyRNA Tissue Kit #AS1390 (Promega Biotech Ibérica, Spain)99. Gene expression changes were detected by relative quantitative reverse

(76) High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistanceNature communicationsSeptember 22, 2022Cortés-López M, Schulz L, Enculescu M, Paret C, Spiekermann B, Quesnel-Vallières M, Torres-Diz M, Unic S, Busch A, Orekhova A, et al.was extracted using the Maxwell RSC Instrument (Promega) and the Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega). 0.5 µg or RNA were reverse-transcribed using the SuperScript™ IV Reverse Transcriptase kit

(77) RNA-Seq Analyses Reveal Roles of the HVCN1 Proton Channel in Cardiac pH HomeostasisFrontiers in Cell and Developmental BiologyMarch 16, 2022Xin Wu, Yawei Li, Mark Maienschein-Cline, Leonid Feferman, Longjun Wu, Liang Hongdescribed (Hong et al., 2021). We first extracted RNA with Maxwell® RSC simplyRNA Cells Kit (Promega AS1390) based on manufacturer’s instructions, using Maxwell® RSC Instrument (Promega AS4500), and then RNA

(78) PIEZO1 mechanoreceptor activation reduces adipogenesis in perivascular adipose tissue preadipocytesFrontiers in endocrinologyJanuary 1, 2022Rendon CJ, Flood E, Thompson JM, Chirivi M, Watts SW, Contreras GA.(RFU). RNA isolation and purification RNA was extracted using the Maxwell® RSC simplyRNA cells kit (AS1390, Promega, Madison, WI) as reported previously (32). Cultured cells were homogenized in 1-Thioglycerol/Homogenization

(79) TP53 wild-type/PPM1D mutant diffuse intrinsic pontine gliomas are sensitive to a MDM2 antagonistActa neuropathologica communicationsNovember 3, 2021Xu C, Liu H, Pirozzi CJ, Chen LH, Greer PK, Diplas BH, Zhang L, Waitkus MS, He Y, Yan H.condition were harvested and RNA extractions were done using Maxwell RSC simplyRNA Cells Kit (Promega #AS1390). RNA samples were sent to for RNA-Seq and the resulting data was analyzed using the Galaxy/Europe platform

(80) TDP-43 condensation properties specify its RNA-binding and regulatory repertoireCellSeptember 2, 2021Hallegger M, Chakrabarti AM, Lee FCY, Lee BL, Amalietti AG, Odeh HM, Copley KE, Rubien JD, Portz B, Kuret K, et al.Green Master Mix,Thermo Fisher Scientific,Cat#4385612 Maxwell RSC simplyRNA Cells Kit,Promega,Cat#AS1390 ,, Deposited data,Deposited data,Deposited data ,, TDP-43 variant iCLIP in Hek-293,This study,"Sequencing

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Maxwell RSC simplyRNA Cells Kit

Maxwell RSC simplyRNA Cells Kit from Promega

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Maxwell RSC simplyRNA Cells Kit from Promega

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