Maxwell RSC simplyRNA Cells Kit AS1390 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Cells Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1390
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFresh or frozen cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) A Titin Missense Variant Causes Atrial FibrillationmedRxivDecember 10, 2024Mahmud Arif Pavel, Hanna Chen, Michael Hill, Arvind Sridhar, Miles Barney, Jaime DeSantiago, Asia Owais, Shashank Sandu, Faisal A. Darbar, Aylin Ornelas-Loredo, et al.western blotting. RNA-Sequencing The RNA was isolated using the Maxwell® RSC simplyRNA Cells Kit (Promega AS1390) according to the instructions provided by the manufacturer. Library preparation was carried out using

(42) Bioactivated Glucoraphanin Improves Cell Survival, Upregulating Phospho-AKT, and Modulates Genes Involved in DNA Repair in an In Vitro Alzheimer’s Disease Model: A Network-Transcriptomic AnalysisNutrientsDecember 5, 2024Aurelio Minuti, Emanuela Mazzon, Renato Iori, Luigi Chiricosta, Osvaldo Artimagnellaprocessed for RNA extraction. Total RNA was extracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument according to the instructions. Afterwards,

(43) Biallelic Germline BRCA1 Frameshift Mutations Associated with Isolated Diminished Ovarian ReserveInternational Journal of Molecular SciencesNovember 20, 2024Anne Helbling-Leclerc, Marie Falampin, Abdelkader Heddar, Léa Guerrini-Rousseau, Maud Marchand, Iphigenie Cavadias, Nathalie Auger, Brigitte Bressac-de Paillerets, Laurence Brugieres, Bernard S. Lopez, et al.was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega, Charbonnières-les-Bains, France, AS1390) and reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Illkirch-Graffenstaden,

(44) Contribution of p53-dependent and -independent mechanisms to upregulation of p21 in Fanconi anemiaPLOS GeneticsNovember 7, 2024Xavier Renaudin, Baraah Al Ahmad Nachar, Benedetta Mancini, Anna Gueiderikh, Noémie Louis-Joseph, Frédérique Maczkowiak-Chartois, Filippo Rossellifrom Dako. qPCR analysis Total RNA was isolated using the Maxwell RSC simplyRNA Cell Kit (Promega, AS1390) and reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, K1622).

(45) Minimally invasive determination of pancreatic ductal adenocarcinoma (PDAC) subtype by means of circulating cell‐free RNAMolecular OncologyOctober 31, 2024Martin Metzenmacher, Gregor Zaun, Marija Trajkovic‐Arsic, Phyllis Cheung, Timm M. Reissig, Hendrik Schürmann, Nils von Neuhoff, Grainne O'Kane, Stephanie Ramotar, Anna Dodd, et al.stored at −80 °C. RNA isolated from cell lines was achieved by use of the SimplyRNA cells kit (cat #AS1390, Promega). All cell lines were authenticated by short tandem repeats (STR) analysis and were confirmed

(46) Characterization of aberrant splicing in pediatric central nervous system tumors reveals CLK1 as a candidate oncogenic dependencybioRxivOctober 8, 2024Ammar S. Naqvi, Ryan J. Corbett, Priyanka Seghal, Karina L. Conkrite, Komal S. Rathi, Brian M. Ennis, Katharina E Hayer, Bo Zhang, Miguel A. Brown, Daniel P. Miller, et al.(qRT-PCR) Total RNA was isolated and treated with DNAse using the Maxwell RSC simplyRNA Cells kit (Promega, AS1390) with the Maxwell RSC48 Instrument (Promega) per the manufacturer’s instructions. Next, 2 μg of RNA

(47) Anti-tumor effects of the eIF4A inhibitor didesmethylrocaglamide and its derivatives in human and canine osteosarcomasScientific ReportsAugust 20, 2024Janet L. Oblinger, Jack Wang, Georgia D. Wetherell, Garima Agarwal, Tyler A. Wilson, Nicole R. Benson, Joelle M. Fenger, James R. Fuchs, A. Douglas Kinghorn, Long-Sheng ChangCA) for RNA-seq analysis. Briefly, RNA was isolated using the Maxwell RSC simplyRNA Cells kit (Cat. #AS1390; Promega). Libraries were prepared with the Illumina TruSeq stranded mRNA kit and sequenced on an Illumina

(48) Purinergic exposure induces epigenomic and transcriptomic-mediated preconditioning resembling epilepsy-associated microglial statesiScienceAugust 16, 2024Martins-Ferreira R, Calafell-Segura J, Chaves J, Ciudad L, Martins da Silva A, Pinho E Costa P, Leal B, Ballestar E.assays ,, CD14+ MicroBeads,Miltenyi Biotec,Cat# 130-050-201 Maxwell RSC simplyRNA Cells Kit,Promega,Cat# AS1390 Transcriptor First Strand cDNA Synthesis Kit,Roche,Cat# 4897030001 LightCycler® 480 SYBR Green Mix,Roche,Cat#

(49) Impact of Nanoplastic Particles on Macrophage Inflammation and Intestinal Health in a Mouse Model of Inflammatory Bowel DiseaseNanomaterials (Basel)August 15, 2024Marlene Schwarzfischer, Tano S. Ruoss, Anna Niechcial, Sung Sik Lee, Marcin Wawrzyniak, Andrea Laimbacher, Kirstin Atrott, Roberto Manzini, Marijn Wilmink, Luise Linzmeier, et al.with PBS twice and scraped off the surface in 200 µL homogenization buffer containing thiogylcerol (#AS1390, Promega, Madison, WI, USA). Bright-field images of the treated cells were taken using a Nikon ECLIPSE Ts2

(50) The PP2A regulator IER5L supports prostate cancer progressionCell Death & DiseaseJuly 18, 2024Jana R. Crespo, Natalia Martín-Martín, Saioa Garcia-Longarte, Jon Corres-Mendizabal, Onintza Carlevaris, Ianire Astobiza, Amaia Zabala-Letona, Marc Guiu, Mikel Azkargorta, Monika Gonzalez-Lopez, et al.quantitative RT-PCR (qRT-PCR) Total RNA was isolated using Maxwell RSC simplyRNA Cells Kit (Promega; AS1390). Complementary DNA was produced from 1 μg of RNA using Maxima™ H Minus cDNA Synthesis Master Mix (Invitrogen;

(51) Cannabinol Regulates the Expression of Cell Cycle-Associated Genes in Motor Neuron-like NSC-34: A Transcriptomic AnalysisBiomedicinesJune 17, 2024Alessandra Trainito, Agnese Gugliandolo, Luigi Chiricosta, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Maria Luidifferentiated cells were harvested and pelleted for RNA extraction. Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) was used to extract the total RNA with a Maxwell® RSC instrument. Library

(52) Cannabinerol Prevents Endoplasmic Reticulum and Mitochondria Dysfunctions in an In Vitro Model of Alzheimer’s Disease: A Network-Based Transcriptomic AnalysisCellsJune 10, 2024Luigi Chiricosta, Aurelio Minuti, Agnese Gugliandolo, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Osvaldo Artimagnellaprocessed for RNA extraction. Total RNA was extracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument. Afterwards, library preparation (from

(53) Peripheral Blood Mononuclear Cell Expression of Cation-Chloride Cotransporter (CCC) Genes in Premenstrual Dysphoric Disorder (PMDD) across the Menstrual Cycle—A Preliminary StudyBiology (Basel)May 25, 2024Soojeong Cho, Fatimata Soumare, Sunni L. Mumford, Paola C. Rosas, Zarema Abrieva, John M. Davis, Ajna Hamidovicdensity gradient centrifugation, following which total RNA was isolated using “Maxwell SimplyRNA Cells Kit (P/N AS1390 Promega, Madison, WI, USA)”, and analyzed with the Real-Time quantitative reverse transcription PCR

(54) Acetyl-CoA carboxylase obstructs CD8+ T cell lipid utilization in the tumor microenvironmentCell MetabolismMay 7, 2024Elizabeth G. Hunt, Katie E. Hurst, Brian P. Riesenberg, Andrew S. Kennedy, Evelyn J. Gandy, Alex M. Andrews, Coral del Mar Alicea del Mar Alicea Pauneto, Lauren E. Ball, Emily D. Wallace, Peng Gao, et al.V4XP-3032 Alt-R Sp Cas9 Nuclease V3,IDT,Cat# 1081059 Maxwell RSC simplyRNA Cells Kit,Promega,Cat# AS1390 Ultra II Directional RNA Library Prep Kit for Illumina,New England Biolabs,Cat# E7760 BODIPY 493/503,Invitrogen,Cat#

(55) A dopamine D1-like receptor-specific agonist improves the survival of septic miceiScienceApril 19, 2024Koichi Tanaka, Mohammed E. Choudhury, Satoshi Kikuchi, Ikuko Takeda, Kensuke Umakoshi, Noriyuki Miyaue, Kanta Mikami, Ayane Takenaga, Harumichi Yagi, Rintaro Shinabe, et al.,Miltenyi Biotec,Catalog number: 130-095-927 Maxwell® RSC simplyRNA Cells / Tissue Kit,Promega,Catalog number: AS1390 RNeasy micro kit,Qiagen,Catalog number: 74004 qPCR RT Master Mix with gDNA Remover,Toyobo,Code number:

(56) Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA moleculesRNAApril 1, 2024Katherine M. McKenney, Robert P. Connacher, Elise B. Dunshee, Aaron C. Goldstrohm500g for 5 min. RNA was purified from the cell pellet using the Maxwell simplyRNA Cells Kit (Promega, AS1390) and Maxwell RSC instrument, following the manufacturer's protocol. For the on-bead DNase digestion,

(57) Oligo-PROTAC strategy for cell-selective and targeted degradation of activated STAT3Molecular Therapy - Nucleic AcidsMarch 12, 2024Jeremy Hall, Zhuoran Zhang, Supriyo Bhattacharya, Dongfang Wang, Marice Alcantara, Yong Liang, Piotr Swiderski, Stephen Forman, Larry Kwak, Nagarajan Vaidehi, et al.protein assays For qPCR, total RNA was extracted from cultured cells using the Maxwell RSC simplyRNA Cells system (AS1390; Promega, Madison, WI), then reverse transcribed into cDNAs with the iScript cDNA synthesis kit (Bio-Rad).

(58) Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic StudyBiomedicinesJanuary 15, 2024Osvaldo Artimagnella, Emanuela Mazzon, Stefano Salamone, Federica Pollastro, Agnese Gugliandolo, Luigi Chiricostaprocessed for RNA extraction. Total RNA was extracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument. Library preparation of two biological

(59) A new physiological medium uncovers biochemical and cellular alterations in Lesch-Nyhan disease fibroblastsMolecular MedicineJanuary 3, 2024Paula Escudero-Ferruz, Neus Ontiveros, Claudia Cano-Estrada, Diane J. Sutcliffe, H. A. Jinnah, Rosa J. Torres, José M. LópezLND fibroblasts cultivated with different media using the Maxwell RSC simply RNA Cells Kit (Promega, AS1390) following the manufacturer’s instructions. This procedure includes DNase I treatment to prevent DNA contamination

(60) Co-targeting HSP90 alpha and CDK7 overcomes resistance against HSP90 inhibitors in BCR-ABL1+ leukemia cellsCell death & diseaseDecember 6, 2023Vogt M, Dienstbier N, Schliehe-Diecks J, Scharov K, Tu JW, Gebing P, Hogenkamp J, Bilen BS, Furlan S, Picard D, et al.Briefly, RNA was isolated utilizing the Maxwell® RSC simplyRNA cells kit (Promega, Madison, WI, USA, #AS1390). Library preparation was carried out following supplier’s guidelines, using the VAHTs Stranded mRNA-Seq

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Maxwell RSC simplyRNA Cells Kit

Maxwell RSC simplyRNA Cells Kit from Promega

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Maxwell RSC simplyRNA Cells Kit from Promega

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