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Maxwell RSC simplyRNA Cells Kit
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Maxwell RSC simplyRNA Cells Kit
Maxwell RSC simplyRNA Cells Kit
Maxwell RSC simplyRNA Cells Kit from Promega
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Citations:
(92)
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Maxwell RSC simplyRNA Cells Kit
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Description
The Maxwell RSC simplyRNA Cells Kit, used with the Maxwell RSC Instrument, provides a simple method for automated RNA purification from fresh or frozen cells. The Maxwell RSC Instrument can process from 1 to 16 samples in under an hour
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC simplyRNA Cells Kit
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS1390
Quantity
48 preps
Source
Cell/Tissue
Nucleic Acid Type
Total RNA
Sample Type
Fresh or frozen cells
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (92)
(21)
Controlled decorin delivery from injectable microgels promotes scarless vocal fold repair
bioRxiv
June 29, 2025
Ryan M. Friedman, Elizabeth A. Brown, Hannah M. Bonelli, Yashna Gupta, Matthew R. Aronson, Kendra McDaid, Hannah Min-Young Oh, Eiman Abu Bandora, Karen B. Zur, Riccardo Gottardi
RNA sequencing
pipeline (12). In short, total RNA was extracted using the Maxwell RSC simply RNA kit (#
AS1390
,
Promega
) in accordance with manufacturer instructions. Nanodrop was used to verify RNA concentration,
(22)
Cannabinol's Modulation of Genes Involved in Oxidative Stress Response and Neuronal Plasticity: A Transcriptomic Analysis
Antioxidants (Basel)
June 17, 2025
Serena Silvestro, Marco Calabrò, Alessandra Trainito, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Aurelio Minuti
(EDTA) solution (#
T4049
,
Sigma Aldrich
, Saint Louis, MO, USA). The Maxwell® RSC simply RNA Cells Kit (#
AS1390
,
Promega
, Milan, IT) was used to extract RNA from NSC-34 cells, following the manufacturer’s instructions.
(23)
Design and screening of novel endosomal escape compounds that enhance functional delivery of oligonucleotides in vitro
Molecular Therapy Nucleic Acids
June 10, 2025
H. Yesid Estupiñán, Tom Baladi, Samantha Roudi, Michael J. Munson, Jeremy Bost, Oskar Gustafsson, Daniel Velásquez-Ramírez, Deepak Kumar Bhatt, Daniel Hagey, Dennis Hekman,
et al.
and RNA isolation. RNA was extracted using the
Maxwell RSC simplyRNA Cells Kit
(
Promega
, catalog no.
AS1390
) and quantified using the
Quant-iT RNA Assay Kit, Broad Range
(
Invitrogen
, catalog no.
Q10213
) following
(24)
Transcriptional analysis of metastatic hormone-naïve prostate cancer primary tumor biopsies reveals a relevant role for SOX11 in prostate cancer cell dissemination
Genome Biol
June 3, 2025
Natalia Martin-Martin, Saioa Garcia-Longarte, Jon Corres-Mendizabal, Uxue Lazcano, Ianire Astobiza, Laura Bozal-Basterra, Nicolas Herranz, Hielke van Splunder, Onintza Carlevaris, Mikel Pujana-Vaquerizo,
et al.
was automatically extracted using the Maxwell RSC (Promega) platform and extraction kits (
Promega
,
AS1390
), following the manufacturer’s instructions. Complementary DNA (cDNA) from 1000 ng of the extracted
(25)
Comprehensive transcriptome assessment in PBMCs of post-COVID patients at a median follow-up of 28 months after a mild COVID infection reveals upregulation of JAK/STAT signaling and a prolonged immune response
Frontiers in Immunology
May 30, 2025
Serena Fineschi, Joakim Klar, Juan Ramon Lopez Egido, Jens Schuster, Jonas Bergquist, René Kaden, Niklas Dahl
was extracted within one week from sampling using the
Maxwell® RSC simplyRNA Cells Kit
(
Promega
, ref.
AS1390
), with the
Maxwell® CSC Instrument
and Software v3.1.0.177 (Promega, 2800 Woods Hollow Road, Madison,
(26)
Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models
Sci Rep
May 21, 2025
Daniela Maria Rasà, Ilaria Stoppa, Noémie Bérenger-Currias, Elena Pasho, Sorana Ciura, Edor Kabashi, Cécile Martinat, Marina Boido
murine tissues and NSC-34 cell lines was performed using Maxwell RSC simply RNA Cells Kit (
Promega
; ref
AS1390
), while for hMNs the RNeasy plus mini kit was used (
QIAGEN
; Venlo, Netherlands; ref
74134
). RNA concentrations
(27)
TLR4 and prostaglandin pathways at the crossroads of endotoxemia-induced lipolysis
Front Immunol
May 19, 2025
Miguel Chirivi, Ursula Abou-Rjeileh, Madison Myers, Jair Parales-Giron, Lynn Worden, Adam L. Lock, G. Andres Contreras
manufacturer’s instructions. For cells, RNA was extracted using the
Maxwell® RSC simplyRNA cells kit
(Cat N°
AS1390
,
Promega
Corp.), following manufacturer protocol. To eliminate genomic DNA, 5 µL of DNase I (Promega)
(28)
Incomplete paralog compensation generates selective dependency on TRA2A in cancer
PLoS Genet
May 14, 2025
Amanda R. Lee, Anna Tangiyan, Isha Singh, Peter S. Choi
TRA2A
and
TRA2B
. Total RNA was extracted from cells using the
Maxwell RSC simplyRNA Cells Kit
(
Promega
#
AS1390
) and the Maxwell RSC48 Instrument (Promega).
RNA-seq
library preparation and paired-end 150-bp
sequencing
(29)
RNA sequencing driven diagnosis expands the phenotypic spectrum of NBAS deficiency
Molecular Genetics and Metabolism
May 1, 2025
Sarah Silverstein, Thomas Cassini, Jiayu Fu, Barbara Pusey, Ellen Macnamara, F Graeme Frost, Charlotte Williams, Yan Huang, Cynthia J. Tifft, Undiagnosed Diseases Network,
et al.
Total RNA was extracted using Maxwell® RSC (
AS4500
,
Promega
) and
Maxwell® RSC simplyRNA Cells Kit
(
AS1390
,
Promega
) following manufacturer’s instructions. The concentrations were determined by Qubit (Thermofisher)
(30)
Mannose and PMI depletion overcomes radiation resistance in HPV-negative head and neck cancer
Cell Commun Signal
April 21, 2025
Tongchuan Wang, Connor Brown, Niamh Doherty, Niall M. Byrne, Rayhanul Islam, Meabh Doherty, Jie Feng, Cancan Yin, Sarah Chambers, Lydia McQuoid,
et al.
analysis Total RNA was extracted from isogenic FaDu and CAL33 cells using the Maxwell RSC simplyRNA (
AS1390
,
Promega
, USA) kit. RNA concentration and purity was assessed by Nanodrop
spectrophotometry
(Thermo
(31)
Hypoxia-induced Wnt5a-secreting fibroblasts promote colon cancer progression
Nat Commun
April 17, 2025
Akikazu Harada, Yoshiaki Yasumizu, Takeshi Harada, Katsumi Fumoto, Akira Sato, Natsumi Maehara, Ryota Sada, Shinji Matsumoto, Takashi Nishina, Kiyoshi Takeda,
et al.
using
Maxwell RSC instrument
with
Maxwell RSC simplyRNA cells kit
(
Promega
, Madison, WI, USA; Cat.
AS1390
). Library preparation was performed using a TruSeq stranded mRNA
Library Prep
kit (Illumina) according
(32)
Perivascular Adipocytes' Adipogenesis Is Defined by Their Anatomical Location in the Descending Thoracic Aorta
Cells
April 11, 2025
G. Andres Contreras, C. Javier Rendon, Alyssa Shadowens, Miguel Chirivi, David Salcedo-Tacuma, D. Adam Lauver, Stephanie W. Watts
instructions. 2.5. RNA Isolation and
Purification
RNA was isolated using the
Maxwell® RSC simplyRNA cells kit
(
AS1390
,
Promega
) according to previously published protocols [12]. The purity, concentration, and integrity
(33)
Comparative performance analysis of neoepitope prediction algorithms in head and neck cancer
Front Immunol
March 4, 2025
Leila Y. Chihab, Julie G. Burel, Aaron M. Miller, Luise Westernberg, Brandee Brown, Jason Greenbaum, Michael J. Korrer, Stephen P. Schoenberger, Sebastian Joyce, Young J. Kim,
et al.
frozen
squamous cell carcinoma of the head and neck
using the Promega Maxwell Simply RNA kit (
Promega
#
AS1390
) and Promega Maxwell 16 nucleic acid extraction automated instrument (
Promega
#
AS2000
).
RNA sequencing
(34)
HHV-6B ribonucleotide reductase sequesters NF-κB subunit p65 to inhibit innate immune responses
iScience
February 21, 2025
Mansaku Hirai, Khoir Amaliin, Jing Rin Huang, Salma Aktar, Yasuko Mori, Jun Arii
4472919
DNeasy Blood & Tissue Kit,
QIAGEN
,Cat #
69504
Maxwell RSC simplyRNA cells kit
,
Promega
,Cat #
AS1390
NEBNext Poly(A) mRNA Magnetic Isolation Module
,
New England Biolabs
,Cat #
E7490L
NEBNext Ultra II Directional
(35)
Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
STAR Protoc
February 6, 2025
Diane Delobel, Hiromi Nishiyori-Sueki, Ilaria Nisoli, Hideya Kawaji, Pauline Robbe, Piero Carninci, Hazuki Takahashi
extraction.a.Extract RNA by your method of choice. Note: We used
Promega
Maxwell simply RNA (catalog number:
AS1390
), but extraction with phenol/chloroform or other methods would be acceptable. 2.RNA quality control.a.
Measure
(36)
Multi-functional near-infrared fluorescent polymer dot–siRNA for gene expression regulation
Journal of Materials Chemistry B
February 5, 2025
Di Sun, Blessing O. Okosun, Yujie Xue, Kirati Tayutivutikul, Kaitlyn H. Smith, Diane C. Darland, Julia X. Zhao
the total RNA extracted using the Maxwell® RSC simplyRNA extraction kit for cells (
Promega
, Cat. #
AS1390
) following manufacturer’s recommended protocol. In brief, cells were lysed in 200 μl of Thioglycerol
(37)
MUC2 expression modulates immune infiltration in colorectal cancer
Frontiers in Immunology
January 24, 2025
Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Jessica Roelands, Davide Bedognetti,
et al.
for RNA extraction as follows. RNA was extracted using
Maxwell® RSC simplyRNA Cells Kit
(
Promega
, #
AS1390
) according to the manufacturer’s recommendations using
Maxwell® RSC Instrument
(
Promega
, #
AS4500
). RNA
(38)
FOXR2 Targets LHX6+/DLX+ Neural Lineages to Drive Central Nervous System Neuroblastoma
Cancer research
January 15, 2025
Jessa S, De Cola A, Chandarana B, McNicholas M, Hébert S, Ptack A, Faury D, Tsai JW, Korshunov A, Phoenix TN,
et al
.
platform. Murine cell lines Total RNA was extracted from cell pellets using
Maxwell RSC simplyRNA Cells Kit
(
AS1390
,
Promega
) according to instructions from the manufacturer. Library preparation was performed with rRNA
(39)
Mitochondrial trifunctional protein deficiency caused by a deep intronic deletion leading to aberrant splicing
JIMD Reports
January 1, 2025
Thomas Cassini, Sarah Silverstein, Molly Behan, Cynthia J. Tifft, May Christine Malicdan, David R. Adams, Undiagnosed Diseases Network, Sun‐Young Ahn, Debra S. Regier
Total RNA was extracted using Maxwell® RSC (
AS4500
,
Promega
) and
Maxwell® RSC simplyRNA Cells Kit
(
AS1390
,
Promega
) following manufacturer's instructions. The concentrations were determined by Qubit (Thermofisher)
(40)
Prediction of key biological processes from intercellular DNA damage differences through model-based fitting
iScience
December 20, 2024
Kensuke Otsuka, Kouki Uchinomiya, Yuki Yaguchi, Atsushi Shibata
was 0.6 Gy/min. Total RNA isolation Total RNA was extracted from cells using the SimplyRNA Kit (#
AS1390
;
Promega
, Madison, WI, USA). The YFP+/FarRed− and YFP−/FarRed+ cells collected from the culture flask
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Maxwell RSC simplyRNA Cells Kit from Promega
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