Maxwell RSC simplyRNA Cells Kit AS1390 from Promega

Product Specs
ItemMaxwell RSC simplyRNA Cells Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1390
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFresh or frozen cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) NRCAM variant defined by microexon skipping is a targetable cell surface proteoform in high-grade gliomasCell ReportsAugust 26, 2025Priyanka Sehgal, Ammar S. Naqvi, Makenna Higgins, Jiageng Liu, Kyra Harvey, Julien Jarroux, Taewoo Kim, Berk Mankaliye, Pamela Mishra, Grace Watterson, et al.were isolated using Maxwell RSC simplyRNA Cells kit with the Maxwell RSC48 Instrument (Promega Cat#AS1390) and reverse-transcribed using SuperScript IV (Invitrogen Cat#18090010). Primer sequences used for cDNA

(22) Computational Splicing Analysis of Transcriptomic Data Reveals Sulforaphane Modulation of Alternative mRNA Splicing of DNA Repair Genes in Differentiated SH-SY5Y NeuronsInternational Journal of Molecular SciencesAugust 23, 2025Maria Lui, Luigi Chiricosta, Renato Iori, Emanuela Mazzon, Aurelio Minuti, Osvaldo ArtimagnellaSubsequently, total RNA was isolated from the cell pellet using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) on the Maxwell® RSC automated extraction system, following the manufacturer’s

(23) Nintedanib Induces Mesenchymal-to-Epithelial Transition and Reduces Subretinal Fibrosis Through Metabolic ReprogrammingInternational journal of molecular sciencesJuly 24, 2025Hughes D, Prestle J, Zippel N, McFetridge S, Szczepan M, Neubauer H, Xu H, Chen M.software version 1.53. 4.5. RNA Extraction Total RNA using the Maxwell RSC simplyRNA Cells Kit (Cat. AS1390, Promega, Seattle, WA, USA) was isolated following manufacturer’s instructions. The concentration and

(24) Tumor Organoids Grown in Mixed-Composition Hydrogels Recapitulate the Plasticity of Pancreatic CancersGelsJuly 21, 2025Ioritz Sorzabal-Bellido, Xabier Morales, Iván Cortés-Domínguez, Maider Esparza, Lucía Grande, Pedro Castillo, Silvia Larumbe, María Monteserín, Shruthi Narayanan, Mariano Ponz-Sarvise, et al.day 7 from mature PDAC93 organoids using the Maxwell RSC SimplyRNA Kit (Promega, Madison, WIS, USA, AS1390) following the manufacturer’s protocol. Complementary DNA was synthesized using the M-MLV Reverse Transcription

(25) Dual targeting of tumoral cells and immune microenvironment by blocking the IL-33/IL1RL1 pathwayNat CommunJuly 14, 2025Denggang Fu, Hua Jiang, Alan Long, Ella Harris, Hongfen Guo, Maegan L. Capitano, John Wrangle, Joshua R. Faust, Anilkumar Gopalakrishnapillai, Santhosh Kumar Pasupuleti, et al.kit. RNA was extracted with a Promega Maxwell RSC 16 using the Maxwell RSC simplyRNA Cells kit (Cat# AS1390) according to the manufacturer’s instructions. RNA concentration was measured using a NanoDrop 8000.

(26) Efficient cell-free translation from diverse human cell typesJournal of Biological ChemistryJuly 1, 2025Jana Ziegelmüller, Nikolaos Kouvelas, Nino Schwaller, Priyanka Thambythurai, Alexander M. Hofer, Oliver Mühlemann, Evangelos D. KarousisMaxwell RSC Instrument (Promega, Cat# AS4500) and the Maxwell RSC simplyRNA Cells Kit (Promega, Cat# AS1390), following the manufacturer’s protocol. RNA concentration after extraction was determined by NanoDrop 2000 spectrophotometer

(27) Controlled decorin delivery from injectable microgels promotes scarless vocal fold repairbioRxivJune 29, 2025Ryan M. Friedman, Elizabeth A. Brown, Hannah M. Bonelli, Yashna Gupta, Matthew R. Aronson, Kendra McDaid, Hannah Min-Young Oh, Eiman Abu Bandora, Karen B. Zur, Riccardo GottardiRNA sequencing pipeline (12). In short, total RNA was extracted using the Maxwell RSC simply RNA kit (#AS1390, Promega) in accordance with manufacturer instructions. Nanodrop was used to verify RNA concentration,

(28) Cannabinol's Modulation of Genes Involved in Oxidative Stress Response and Neuronal Plasticity: A Transcriptomic AnalysisAntioxidants (Basel)June 17, 2025Serena Silvestro, Marco Calabrò, Alessandra Trainito, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Aurelio Minuti(EDTA) solution (#T4049, Sigma Aldrich, Saint Louis, MO, USA). The Maxwell® RSC simply RNA Cells Kit (#AS1390, Promega, Milan, IT) was used to extract RNA from NSC-34 cells, following the manufacturer’s instructions.

(29) Design and screening of novel endosomal escape compounds that enhance functional delivery of oligonucleotides in vitroMolecular Therapy Nucleic AcidsJune 10, 2025H. Yesid Estupiñán, Tom Baladi, Samantha Roudi, Michael J. Munson, Jeremy Bost, Oskar Gustafsson, Daniel Velásquez-Ramírez, Deepak Kumar Bhatt, Daniel Hagey, Dennis Hekman, et al.and RNA isolation. RNA was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega, catalog no. AS1390) and quantified using the Quant-iT RNA Assay Kit, Broad Range (Invitrogen, catalog no. Q10213) following

(30) Transcriptional analysis of metastatic hormone-naïve prostate cancer primary tumor biopsies reveals a relevant role for SOX11 in prostate cancer cell disseminationGenome BiolJune 3, 2025Natalia Martin-Martin, Saioa Garcia-Longarte, Jon Corres-Mendizabal, Uxue Lazcano, Ianire Astobiza, Laura Bozal-Basterra, Nicolas Herranz, Hielke van Splunder, Onintza Carlevaris, Mikel Pujana-Vaquerizo, et al.was automatically extracted using the Maxwell RSC (Promega) platform and extraction kits (Promega, AS1390), following the manufacturer’s instructions. Complementary DNA (cDNA) from 1000 ng of the extracted

(31) Comprehensive transcriptome assessment in PBMCs of post-COVID patients at a median follow-up of 28 months after a mild COVID infection reveals upregulation of JAK/STAT signaling and a prolonged immune responseFrontiers in ImmunologyMay 30, 2025Serena Fineschi, Joakim Klar, Juan Ramon Lopez Egido, Jens Schuster, Jonas Bergquist, René Kaden, Niklas Dahlwas extracted within one week from sampling using the Maxwell® RSC simplyRNA Cells Kit (Promega, ref. AS1390), with the Maxwell® CSC Instrument and Software v3.1.0.177 (Promega, 2800 Woods Hollow Road, Madison,

(32) Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental modelsSci RepMay 21, 2025Daniela Maria Rasà, Ilaria Stoppa, Noémie Bérenger-Currias, Elena Pasho, Sorana Ciura, Edor Kabashi, Cécile Martinat, Marina Boidomurine tissues and NSC-34 cell lines was performed using Maxwell RSC simply RNA Cells Kit (Promega; ref AS1390), while for hMNs the RNeasy plus mini kit was used (QIAGEN; Venlo, Netherlands; ref 74134). RNA concentrations

(33) TLR4 and prostaglandin pathways at the crossroads of endotoxemia-induced lipolysisFront ImmunolMay 19, 2025Miguel Chirivi, Ursula Abou-Rjeileh, Madison Myers, Jair Parales-Giron, Lynn Worden, Adam L. Lock, G. Andres Contrerasmanufacturer’s instructions. For cells, RNA was extracted using the Maxwell® RSC simplyRNA cells kit (Cat N° AS1390, Promega Corp.), following manufacturer protocol. To eliminate genomic DNA, 5 µL of DNase I (Promega)

(34) Incomplete paralog compensation generates selective dependency on TRA2A in cancerPLoS GenetMay 14, 2025Amanda R. Lee, Anna Tangiyan, Isha Singh, Peter S. ChoiTRA2A and TRA2B. Total RNA was extracted from cells using the Maxwell RSC simplyRNA Cells Kit (Promega #AS1390) and the Maxwell RSC48 Instrument (Promega). RNA-seq library preparation and paired-end 150-bp sequencing

(35) RNA sequencing driven diagnosis expands the phenotypic spectrum of NBAS deficiencyMolecular Genetics and MetabolismMay 1, 2025Sarah Silverstein, Thomas Cassini, Jiayu Fu, Barbara Pusey, Ellen Macnamara, F Graeme Frost, Charlotte Williams, Yan Huang, Cynthia J. Tifft, Undiagnosed Diseases Network, et al.Total RNA was extracted using Maxwell® RSC (AS4500, Promega) and Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega) following manufacturer’s instructions. The concentrations were determined by Qubit (Thermofisher)

(36) Mannose and PMI depletion overcomes radiation resistance in HPV-negative head and neck cancerCell Commun SignalApril 21, 2025Tongchuan Wang, Connor Brown, Niamh Doherty, Niall M. Byrne, Rayhanul Islam, Meabh Doherty, Jie Feng, Cancan Yin, Sarah Chambers, Lydia McQuoid, et al.analysis Total RNA was extracted from isogenic FaDu and CAL33 cells using the Maxwell RSC simplyRNA (AS1390, Promega, USA) kit. RNA concentration and purity was assessed by Nanodrop spectrophotometry (Thermo

(37) Hypoxia-induced Wnt5a-secreting fibroblasts promote colon cancer progressionNat CommunApril 17, 2025Akikazu Harada, Yoshiaki Yasumizu, Takeshi Harada, Katsumi Fumoto, Akira Sato, Natsumi Maehara, Ryota Sada, Shinji Matsumoto, Takashi Nishina, Kiyoshi Takeda, et al.using Maxwell RSC instrument with Maxwell RSC simplyRNA cells kit (Promega, Madison, WI, USA; Cat. AS1390). Library preparation was performed using a TruSeq stranded mRNA Library Prep kit (Illumina) according

(38) Perivascular Adipocytes' Adipogenesis Is Defined by Their Anatomical Location in the Descending Thoracic AortaCellsApril 11, 2025G. Andres Contreras, C. Javier Rendon, Alyssa Shadowens, Miguel Chirivi, David Salcedo-Tacuma, D. Adam Lauver, Stephanie W. Wattsinstructions. 2.5. RNA Isolation and Purification RNA was isolated using the Maxwell® RSC simplyRNA cells kit (AS1390, Promega) according to previously published protocols [12]. The purity, concentration, and integrity

(39) Comparative performance analysis of neoepitope prediction algorithms in head and neck cancerFront ImmunolMarch 4, 2025Leila Y. Chihab, Julie G. Burel, Aaron M. Miller, Luise Westernberg, Brandee Brown, Jason Greenbaum, Michael J. Korrer, Stephen P. Schoenberger, Sebastian Joyce, Young J. Kim, et al.frozen squamous cell carcinoma of the head and neck using the Promega Maxwell Simply RNA kit (Promega #AS1390) and Promega Maxwell 16 nucleic acid extraction automated instrument (Promega #AS2000). RNA sequencing

(40) HHV-6B ribonucleotide reductase sequesters NF-κB subunit p65 to inhibit innate immune responsesiScienceFebruary 21, 2025Mansaku Hirai, Khoir Amaliin, Jing Rin Huang, Salma Aktar, Yasuko Mori, Jun Arii4472919 DNeasy Blood & Tissue Kit,QIAGEN,Cat # 69504 Maxwell RSC simplyRNA cells kit,Promega,Cat # AS1390 NEBNext Poly(A) mRNA Magnetic Isolation Module,New England Biolabs,Cat # E7490L NEBNext Ultra II Directional

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Maxwell RSC simplyRNA Cells Kit

Maxwell RSC simplyRNA Cells Kit from Promega

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Maxwell RSC simplyRNA Cells Kit from Promega

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