ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Cellular mechanism of action of 2-nitroimidazoles as hypoxia-selective therapeutic agentsRedox biologyJune 1, 2022Rashed FB, Diaz-Dussan D, Mashayekhi F, Macdonald D, Nation PN, Yang XH, Sokhi S, Stoica AC, El-Saidi H, Ricardo C, et al.Corp., Sumerduck, VA). 2.14 Assessing hydrogen peroxide (H2O2) levels The ROS-Glo™ H2O2 assay kit (G8820, Promega, Madison, WI) was used to analyze the levels of H2O2 as a readout of the reactive oxygen species

(82) A topological refactoring design strategy yields highly stable granulopoietic proteinsNature communicationsMay 26, 2022Skokowa J, Hernandez Alvarez B, Coles M, Ritter M, Nasri M, Haaf J, Aghaallaei N, Xu Y, Mir P, Krahl AC, et al.peroxide (H2O2), a reactive oxygen species (ROS), was measured with the ROS-Glo H2O2 Assay kit (Promega, #G8820) according to the manufacturer’s protocol. Quantitative analysis of NETosis formation Ex vivo generated

(83) Identification of Synergistic Drug Combinations to Target KRAS-Driven Chemoradioresistant Cancers Utilizing Tumoroid Models of Colorectal Adenocarcinoma and Recurrent GlioblastomaFrontiers in OncologyMay 18, 2022Kshama Gupta, Jeremy C. Jones, Virginea De Araujo Farias, Yuri Mackeyev, Pankaj K. Singh, Alfredo Quiñones-Hinojosa, Sunil KrishnanSpheroid growth and health were evaluated based on the increase in ROS levels (ROS-Glo™ H2O2 Assay, G8820, Promega), change in spheroid cell viability (CellTiter-Glo®, G7572, Promega), and induction of caspase

(84) Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammationCellMay 12, 2022Wei TT, Chandy M, Nishiga M, Zhang A, Kumar KK, Thomas D, Manhas A, Rhee S, Justesen JM, Chen IY, et al.2000,Thermo Fisher Scientific,11668019 CellTiter-Glo 2.0 Assay,Promega,G9241 ROS-Glo™ H2O2 Assay,Promega,G8820 GTPase-Glo™ Assay,Promega,V7681 "Cell ROX™ Deep Red Reagent, for oxidative stress detection",Thermo Fisher Scientific

(85) NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulenceBMC veterinary researchApril 2, 2022Hao F, Xie X, Feng Z, Chen R, Wei Y, Liu J, Xiong Q, Shao G, Lin J.independent replicates. Reactive oxygen species (ROS) were measured using a ROS-Glo™ H2O2 Assay (Cat No. G8820, Promega, Madison, WI, USA) according to the manufacturer’s instructions, and RLUs were recorded using

(86) DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial DysfunctionInternational journal of molecular sciencesMarch 6, 2022Le AN, Park SS, Le MX, Lee UH, Ko BK, Lim HR, Yu R, Choi SH, Lee BJ, Ham SY, et al.Shinjuku, Japan). 4.18. Measurement of ROS Levels H2O2 level was measured using a ROS-Glo H2O2 Assay (G8820, Promega) according to the protocol supplied by the manufacturer. Briefly, 1 × 104 cells/well were seeded

(87) Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survivalScience advancesFebruary 25, 2022Whitburn J, Rao SR, Morris EV, Tabata S, Hirayama A, Soga T, Edwards JR, Kaya Z, Palmer C, Hamdy FC, et al.to measure mitochondrial superoxide. Total ROS levels were measured using a ROS-Glo Kit (catalog no. G8820, Promega) and glutathione levels were measured using a GSH Glo kit (catalog no. V6911, Promega, Wisconsin,

(88) The diaryl-imidazopyridazine anti-plasmodial compound, MMV652103, exhibits anti-breast cancer activityEXCLI journalJanuary 1, 2022Neumann-Mufweba A, Kimani S, Khan SF, Chibale K, Prince S.stress in breast cancer cells, the levels of H2O2 were measured using the ROS-Glo™ H2O2 assay kit (G8820, Promega, Wisconsin, USA), according to the manufacturer's instructions. Briefly, cells (2500-4500 cells

(89) RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapyCell stem cellDecember 2, 2021Magdy T, Jiang Z, Jouni M, Fonoudi H, Lyra-Leite D, Jung G, Romero-Tejeda M, Kuo HH, Fetterman KA, Gharib M, et al.Array,Illumina,WG-320-2101 CellTiter-Glo 2.0,Promega,G9242 Caspase 3/7-Glo,Promega,G8091 ROS-Glo H2O2,Promega,G8820 JC-10,Enzo Life Sciences,ENZ-52305 TruSeq RNA Library Prep Kit v2,Illumina,RS-122-2001 Deposited

(90) A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagyRedox biologyDecember 1, 2021Zeb A, Choubey V, Gupta R, Kuum M, Safiulina D, Vaarmann A, Gogichaishvili N, Liiv M, Ilves I, Tämm K, et al.[70]. 4.9 ROS production To measure ROS induced by menadione, we used ROS-Glo™ H2O2 assay (Promega, G8820). PC6 cells were grown overnight on an opaque white 96 well plate. On the next day, the cells were treated

(91) Complex II subunit SDHD is critical for cell growth and metabolism, which can be partially restored with a synthetic ubiquinone analogBMC Molecular and Cell BiologyDecember 1, 2021Aloka B. Bandara, Joshua C. Drake, David A. Browntriplicates. Generation of reactive oxygen species (ROS) was measured using ROS-Glo H2O2 Assay (Cat# G8820; Promega Corporation, Madison, WI). Apoptosis and necrosis were assessed using RealTime-Glo Annexin V Apoptosis and Necrosis Assay

(92) Vitamin D-Mediated Anti-cancer Activity Involves Iron Homeostatic Balance Disruption and Oxidative Stress Induction in Breast CancerFrontiers in Cell and Developmental BiologyNovember 8, 2021Khuloud Bajbouj, Lina Sahnoon, Jasmin Shafarin, Abeer Al-Ali, Jibran Sualeh Muhammad, Asima Karim, Salman Y. Guraya, Mawieh HamadMeasurement Antioxidant related enzymes were measured using the ROS-Glo H2O2 Assay Kit (Promega, Cat No. G8820, United States) in both MDMBA-321 and MCF-7 cells, 5,500 cells/well were seeded using a 96-well plate

(93) PCNA inhibition enhances the cytotoxicity of β-lapachone in NQO1-Positive cancer cells by augmentation of oxidative stress-induced DNA damageCancer lettersOctober 28, 2021Su X, Wang J, Jiang L, Chen Y, Lu T, Mendonca MS, Huang X.during or after treatments using standard chemical assays following manufactures’ protocols. (Promega G8820, Promega G9071, Promega G7572). 2.9. PCNA knockdown by siRNA PCNA-specific siRNA ( CCUUGGCGCUAGUAUUUGAtt,

(94) Structural basis and regulation of the reductive stress responseCellOctober 14, 2021Manford AG, Mena EL, Shih KY, Gee CL, McMinimy R, Martínez-González B, Sherriff R, Lew B, Zoltek M, Rodríguez-Pérez F, et al.ROS levels were measured. H2O2 was measured from growth media using the ROS-Glo H2O2 Assay (Promega, G8820) according the manufactures protocol. Luminescence was measured with a Perkin-Elmer Envision Multilabel

(95) A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial FibroblastsCellsOctober 9, 2021Janczi T, Meier F, Fehrl Y, Kinne RW, Böhm B, Burkhardt H.Germany). 2.9. Reactive Oxygen Species (ROS) Assays ROS were measured using the ROS-Glo H2O2 Assay (#G8820) from Promega. Briefly, after cyclic straining, cells were scraped together, and 5 × 103 cells (in 80

(96) Dietary fructose improves intestinal cell survival and nutrient absorptionNatureSeptember 1, 2021Taylor SR, Ramsamooj S, Liang RJ, Katti A, Pozovskiy R, Vasan N, Hwang SK, Nahiyaan N, Francoeur NJ, Schatoff EM, et al.Winooski, VT). Total cell ROS measurements were performed using the ROS-Glo H2O2 assay (Promega, Cat. #G8820) per the manufacturer’s instructions. Values from normoxic and hypoxic plates were compared after correcting

(97) A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activationScience signalingAugust 3, 2021Ernst O, Sun J, Lin B, Banoth B, Dorrington MG, Liang J, Schwarz B, Stromberg KA, Katz S, Vayttaden SJ, et al.ng/ml LPS and ROS production was measured using the hydrogen peroxide-based ROS-glo assay kit (Promega, G8820), according to the manufacturer’s protocol. Single-cell ROS measurements were conducted in RAW264.7

(98) MUC1-C activates the PBAF chromatin remodeling complex in integrating redox balance with progression of human prostate cancer stem cellsOncogeneJuly 29, 2021Masayuki Hagiwara, Atsushi Fushimi, Nami Yamashita, Atrayee Bhattacharya, Hasan Rajabi, Mark D. Long, Yota Yasumizu, Mototsugu Oya, Song Liu, Donald Kufewells. Measurements of ROS, NADP/NADPH, GSH, GSH/GSSG levels. Assays for measurements of ROS (ROS-Glo H2O2 Assay, G8820; Promega, Madison, WI, USA), NADP/NADPH (NADP/NADPH-Glo Assay, G9081; Promega), GSH (GSH-Glo Glutathione Assay

(99) Complex I protein NDUFS2 is vital for growth, ROS generation, membrane integrity, apoptosis, and mitochondrial energeticsMitochondrionMay 1, 2021Aloka B. Bandara, Joshua C. Drake, Carissa C. James, James W. Smyth, David A. Brownviable cells, relative to non-viable cells. ROS generation was measured using ROS-Glo H2O2 Assay (Cat# G8820; Promega). Apoptosis and necrosis were assessed using RealTime-Glo Annexin V Apoptosis and Necrosis Assay

(100) In vitro toxicity of fibrous glaucophaneToxicologyApril 1, 2021Alessandro F. Gualtieri et al.fibres. Positive con trols were: 100 μg/mL crocidolite and 500 μM H2O2. ROS-Glo H2O2 assay (Promega G8820) was used to measure H2O2 levels in Met-5A and A549 cell line treated with glaucophane and crocidolite.

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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