ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signalingNat CommunOctober 8, 2025Jung Seok Hwang, JiHoon Kang, Jaehyun Kim, Kiyoung Eun, Sophia West, Hannah E. Bacho, Vanessa Avalos, Sydney Shuff, Dong M. Shin, Nabil F. Saba, et al.(89874) was obtained from Pierce. MitoTracker Red CMXros (M7512) was from Invitrogen. ROS-Glo H2O2 Assay (G8820), NADP/NADPH-Glo Assay (G9081), and GSH/GSSG-Glo Assay (V6611) were procured from Promega. MtioSOX (M36008)

(22) Spinocerebellar Ataxia Type 1 (SCA1) Cell Models Display Widespread Mitochondrial and Extra-Nuclear AlterationsJ Mol NeurosciOctober 2, 2025Dane Ford-Roshon, Madison Dudek, Ada Glynn, Abigale Glasman, Jaden York, Emily Lawrence, Donna Nguyen, Lindsay Shinn, Georgia Berry, Lily Kendall, et al.long half-life, was measured in WT Daoy and ATXN1[82Q] cells using the ROS-Glo H2O2 Assay kit (Promega G8820). Harvested cells were plated at a concentration of 3000 Daoy cells or 5000 ATXN1[82Q] cells, to account

(23) The NLRP3 Inflammasome Transmits Sterile Inflammation Signals to Sustain Proper Mitochondrial Electron Transport Chain Function and Influences Cellular MetabolismStem Cell Reviews and ReportsOctober 1, 2025Adrian Konopko, Michalina Kazek, Emilia Waraksa-Zasada, Agnieszka Łukomska, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczakand Nlrp3-KO BM was measured using the ROS-Glo™ H2O2 Assay (Promega, Madison, WI, USA, Catalog No. G8820) following the manufacturer’s instructions. Control cells (untreated) and cells treated with eATP (1

(24) Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12Cell ReportsSeptember 23, 2025Andrew J. Ingersoll, Devlon M. McCloud, Jenny Y. Hu, Michael Rapeshown. ROS measurements Cellular levels of H2O2 were determined using the ROS-Glo™ H2O2 Assay (Promega, G8820) according to manufacturer’s instructions. qPCR C2C12 cells were transfected with siRNAs and RNA was

(25) Mechanistic Insights into the Anticancer Action of Novel 2-Hydroxy-1,4-naphthoquinone Thiol DerivativesACS OmegaSeptember 16, 2025Thaís Barreto Santos, Alex de Souza Cruz Lopes Canuto, João Francisco Blaudt Virgilio de Carvalho Meira, Ana Caroline Santos-Diniz, Rafaella Machado de Assis Cabral Ribeiro, Caroline Reis Santiago Paschoal, Vitor Won-Held Rabelo, Paula Alvarez Abreu, Vitor Francisco Ferreira, David Rodrigues da Rocha, et al.then incubated at 37 °C with 5% CO2 for the indicated time, and ROS was measured by ROS-Glo H2O2 Assay G8820 (Promega Corporation, Madison, WI 53711 USA) as indicated by the vendor. The luminescence signal was

(26) Exposure to Bisphenol S and Bisphenol F Alters Gene Networks Related to Protein Translation and Neuroinflammation in SH-SY5Y Human Neuroblastoma CellsToxicsSeptember 12, 2025Andrea P. Guzman, Christina L. Sanchez, Emma Ivantsova, Jacqueline Watkins, Sara E. Sutton, Christopher L. Souders, Christopher J. MartyniukSupplemental Methods. ROS production was measured using (1) the ROS-Glo™ H2O2 Assay (Promega, Cat #G8820) as per the manufacturer’s protocol and (2) with the fluorescent probe DCFDA (2′,7′-dichlorofluorescein

(27) Sodium-glucose cotransporter 2 inhibitors ameliorate glutathione cysteine ligase modifier-mediated oxidative stress and subsequent ferroptosis in proximal tubules of diabetic kidney diseaseRedox RepJuly 29, 2025Yi-Chun Tsai, Jiun-Chi Huang, Ping-Shaou Yu, Mei-Chuan Kuo, Ling-Yu Wu, Wei-An Chang, Shang-Jyh Hwang, Ya-Ling Hsuwavelengths of 380 and 485 nm, respectively. To assess the ROS levels, the ROS-Glo H2O2 Assay system (Cat No. G8820, Promega, WI, USA) was used according to the manufacturer’s instructions. Cultured cells were trypsinized,

(28) Peptide YY fragment PYY1-36 disrupts mitochondrial biogenesis via RBM43-dependent PGC-1α translation inhibitionInvestigational New DrugsJune 1, 2025Benkun Liu, Fucheng Zhou, Bowen Shi, Yubo Yan, Yanbo Wang, Junfeng Wang, Yaoguo Lang, Shidong XuN-acetylcysteine, NAC, Sigma-Aldrich, A7250, for 1 h before 100 nM PYY1-36). The ROS-Glo H₂O₂ Assay (Promega, G8820) was performed per the manufacturer’s protocol, as an alternative to flow cytometry due to poor-quality

(29) IL-6-mediated tumorigenicity and antioxidant state in squamous cell carcinoma cells are driven by CD109 via stabilization of IL-6 receptor-alpha and activation of STAT3/NRF2 pathwayExp Hematol OncolMay 2, 2025Amani Hassan, Tenzin Kungyal, Shufeng Zhou, Meryem Blati, Kenneth Finnson, Nick Bertos, Nahid Golabi, Nader Sadeghi, Sampath Loganathan, Anie PhilipROS-Glo™ H2O2 assay ROS levels were measured using ROS-Glo™ H2O2 Assay kit from Promega (Promega; # G8820) according to manufacturer’s instructions. A431WT, SCC9 WT and their corresponding CD109 KO cells were

(30) Exploiting the therapeutic vulnerability of IDH-mutant gliomas with zotiraciclibiScienceApril 18, 2025Ying Pang, Qi Li, Zach Sergi, Guangyang Yu, Olga Kim, Peng Lu, Marina Chan, Xueyu Sang, Herui Wang, Alice Ranjan, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, ROS-Glo H2O2 assay kit,Promega,G8820 Caspase-Glo 3/7 Assay Kit,Promega,G8091 Seahorse XF Cell Mito Stress Test Kit,Agilent,103015-100 Seahorse

(31) Signaling and transcriptional dynamics underlying early adaptation to oncogenic BRAF inhibitionCell SystemsApril 16, 2025Cameron T. Flower, Chunmei Liu, Hui-Yu Chuang, Xiaoyang Ye, Hanjun Cheng, James R. Heath, Wei Wei, Forest M. Whitehour prior to ROS quantitation. ROS levels were quantified by ROS-Glo H2O2 Assay (Promega, catalog #G8820) according to the manufacturer’s protocol. The luminescence signals were measured by the Synergy H4

(32) Chaperone-mediated autophagy regulates the metastatic state of mesenchymal tumorsEMBO Molecular MedicineMarch 7, 2025Xun Zhou, Eva Berenger, Yong Shi, Vera Shirokova, Elena Kochetkova, Tina Becirovic, Boxi Zhang, Vitaliy O Kaminskyy, Yashar Esmaeilian, Kayoko Hosaka, et al.Test Kit,Seahorse Bioscience,103010-100 G6PDH Assay Kit,Abcam,ab102529 ROS-Glo H2O2 assay,Promega,G8820 Software,Software,Software R (v 4.0.5),https://www.r-project.org/, tximport package (v 1.26.1),https://bioconductor.org/packages/release/bioc/html/tximport.html, clusterProfiler

(33) Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cellsBiol ResFebruary 21, 2025Anna Szewczyk, Nina Rembiałkowska, Jolanta Saczko, Małgorzata Daczewska, Vitalij Novickij, Julita Kulbackaviable cells relative to untreated controls. ROS level investigation The ROS-Glo™ H₂O₂ Assay (Promega, G8820) is a sensitive bioluminescent assay that directly measures cell culture’s reactive oxygen species (ROS)

(34) The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cellsFront ImmunolFebruary 6, 2025Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, et al.fluorescence and luminescence measurements. Levels of H2O2 were measure using the ROS-Glo H2O2 assay (G8820, Promega) and assay was performed according to manufacturer instructions. Briefly, cells were exposed

(35) RNF7‐Mediated ROS Targets Malignant Phenotype and Radiotherapy Sensitivity in Glioma With Different IDH1 GenotypesMolecular CarcinogenesisJanuary 9, 2025Yiran Tao, Zimin Shi, Xianyin Liang, Yuqian Zheng, Lirui Dai, Xiang Li, Zian Li, Wulong Liang, Gaojie Bai, Hao Li, et al.using the Reactive Oxygen Species Assay Kit (Beyotime, S0033S) or the ROS‐Glo H2O2 assay kit (Promega, G8820). Following the manufacturer's protocols, the treated samples were examined using a fluorescence microscope

(36) Evaluating the Antibacterial Potential of Distinct Size Populations of Stabilized Zinc NanoparticlesACS Applied Materials & InterfacesJanuary 8, 2025Dinny Stevens, Amanda K. Charlton-Sevcik, W. Evan Braswell, Christie M. Sayesh exposure was measured through H2O2 production using the Luminescent ROS-Glo H2O2 assay (Promega, #G8820). The positive control for ROS production was 200 μM menadione (Thermo Fisher Scientific). Bacteria

(37) Pharmacological Vitamin C-induced high H2O2 generation mediates apoptotic cell death by caspase 3/7 activation in breast cancer tumor spheroidsJ Transl MedJanuary 8, 2025Ali Mussa, Mahasin Hamid, Khalid Hajissa, Ahmad Hafiz Murtadha, Mohammad A. I. Al-Hatamleh, Noor Fatmawati Mokhtar, Vuk Uskoković, Magdalena Plebanski, Rohimah Mohamud, Rosline HassanVit-C doses for 72 h, the cellular ROS was determined by performing the ROS-GLO™ H2O2 ASSAY (PROYY-G8820, Promega, USA) according to the protocol from the manufacturer. In short, from the H2O2 substrate solution

(38) β-Lapachone promotes the recruitment and polarization of tumor-associated neutrophils (TANs) toward an antitumor (N1) phenotype in NQO1-positive cancersOncoImmunologyDecember 31, 2024Soumya Tumbath, Lingxiang Jiang, Xiaoguang Li, Taolan Zhang, Kashif Rafiq Zahid, Ye Zhao, Hao Zhou, Zhijun Yin, Tao Lu, Shu Jiang, et al.and anti-HMGB1 monoclonal antibodies were purchased from BioXCell (Lebanon, NH). ROS-GloTM assay kit (G8820) was obtained from Promega (Madison, WI). TACSTM Annexin V-FITC apoptosis detection kit was purchased

(39) Efficacy of glutathione inhibitor eprenetapopt against the vulnerability of glutathione metabolism in SMARCA4-, SMARCB1- and PBRM1-deficient cancer cellsScientific ReportsDecember 28, 2024Mariko Sasaki, Hideaki OgiwaraGSH/GSSG-Glo Assay (Promega, V6611), the GSH-Glo Assay (Promega, V6911), the ROS-Glo H2O2 Assay (Promega, G8820), and the Caspase-Glo 3/7 Assay (Promega, G8091), respectively. To measure levels of GSH/GSSG, ROS,

(40) Reactive oxygen species control protein degradation at the mitochondrial import gateMolecular CellDecember 5, 2024Rachael McMinimy, Andrew G. Manford, Christine L. Gee, Srividya Chandrasekhar, Gergey Alzaem Mousa, Joelle Chuang, Lilian Phu, Karen Y. Shih, Christopher M. Rose, John Kuriyan, et al.commercial assays,Critical commercial assays,Critical commercial assays ROS-Glo H2O2 Assay,Promega,Cat#G8820 Ionic Detergent Compatibility Reagent,Thermo Fisher Scientific,Cat#22663 Pierce 660nm Protein Assay Reagent

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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