ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) A Novel Class of FKBP12 Ligands Rescues Premature Aging Phenotypes Associated with Myotonic Dystrophy Type 1CellsNovember 22, 2024Mikel García-Puga, Gorka Gerenu, Ariadna Bargiela, Jorge Espinosa-Espinosa, Laura Mosqueira-Martín, Maialen Sagartzazu-Aizpurua, Jesús M. Aizpurua, Ainara Vallejo-Illarramendi, Rubén Artero, Adolfo López de Munain, et al.and, after 14–16 h, was treated with 0.1 µM MP compounds for 72 h. Afterwards, ROS-Glo H2O2 Assay (G8820, Promega, Madison, WI, USA) was performed according to the manufacturer’s instructions to measure H2O2

(42) CCL2-mediated endothelial injury drives cardiac dysfunction in long COVIDNature Cardiovascular ResearchOctober 14, 2024Dilip Thomas, Chikage Noishiki, Sadhana Gaddam, David Wu, Amit Manhas, Yu Liu, Dipti Tripathi, Nimish Kathale, Shaunak S. Adkar, Jaishree Garhyan, et al.were used. Production of ROS ROS levels in COs were determined using ROS-Glo H2O2 Assay (Promega, G8820) as per the manufacturer’s instructions. In brief, equal proportions of COs that were treated with vehicle

(43) Anti-Proliferative and Anti-Migratory Activity of Licorice Extract and Glycyrrhetinic Acid on Papillary Thyroid Cancer Cell CulturesInternational Journal of Molecular SciencesOctober 8, 2024Jacopo Manso, Simona Censi, Maria Chiara Pedron, Loris Bertazza, Alberto Mondin, Edoardo Ruggeri, Susi Barollo, Chiara Sabbadin, Isabella Merante Boschin, Decio Armanini, et al.made through the Reactive Oxygen Species (ROS)-Glo™ H2O2 Assay (Promega, Madison, WI, USA; Cat. No. G8820) following the manufacturer’s instructions. Briefly, cells were seeded and treated for 1 h, as described

(44) Cytotoxicity, Proapoptotic Activity and Drug-like Potential of Quercetin and Kaempferol in Glioblastoma Cells: Preclinical InsightsInternational Journal of Molecular SciencesOctober 5, 2024Magdalena Kusaczuk, Elena Tovar-Ambel, Paola Martín-Cabrera, Mar Lorente, Nélida Salvador-Tormo, Agnieszka Mikłosz, Adrian Chabowski, Guillermo Velasco, Monika NaumowiczCellTiter-Glo luminescent cell-viability assay (G7570), the Caspase-Glo 3/7 assay (G8981), the Caspase-Glo 9 assay, and the ROS-Glo H2O2 system (G8820) were purchased from Promega (Fitchburg, WI, USA). The Protease/Phosphatase Inhibitor Cocktail (#5872S),

(45) Oncometabolite 2-hydroxyglutarate suppresses basal protein levels of DNA polymerase beta that enhances alkylating agent and PARG inhibition induced cytotoxicityDNA Repair (Amst)August 1, 2024Kate M. Saville, Rasha Q. Al-Rahahleh, Aisha H. Siddiqui, Morgan E. Andrews, Wynand P. Roos, Christopher A. Koczor, Joel F. Andrews, Faisal Hayat, Marie E. Migaud, Robert W. Sobol0, 1, 2, 4, 6, or 8 hours. ROS was determined using the Promega ROS-Glo hydrogen peroxide kit (Cat# G8820) and was carried out according to the manufacturer’s instructions. All analyses were performed in triplicate

(46) Inhibition of PHLDA3 expression in human superoxide dismutase 1-mutant amyotrophic lateral sclerosis astrocytes protects against neurotoxicityBrain CommunicationsJuly 25, 2024Kornélia Szebényi, Ingrid Vargová, Veselina Petrova, Jana Turečková, George M Gibbons, Monika Řehořová, Mai Abdelgawad, Alexandra Sándor, Dana Marekova, Jessica C F Kwok, et al.transfected astrocytes and NeuN+ neurons. The reactive oxygen species (ROS)-Glo H2O2 assay (Promega, G8820) was used to detect ROS levels in astrocyte-conditioned media (ACM), indicated by the H2O2 luminescence

(47) S100A1’s single cysteine is an indispensable redox switch for the protection against diastolic calcium waves in cardiomyocytesAmerican Journal of Physiology-Heart and Circulatory PhysiologyJuly 1, 2024Andreas Seitz, Martin Busch, Jasmin Kroemer, Andrea Schneider, Stephanie Simon, Andreas Jungmann, Hugo A. Katus, Patrick Most, Julia RitterhoffBiotin-maleimide (B1267, Sigma-Aldrich), N-ethylmaleimide (E3876, Sigma-Aldrich), ROS-Glo H2O2 Assay (Promega, G8820). All other chemicals were obtained from Sigma-Aldrich, Germany, if not indicated specifically. Generation

(48) Liver X Receptor Ligand GAC0001E5 Downregulates Antioxidant Capacity and ERBB2/HER2 Expression in HER2-Positive Breast Cancer CellsCancersApril 25, 2024Premaratne A, Basu S, Bagchi A, Zhou T, Feng Q, Lin CY.to corresponding cell numbers. 2.10. ROS Levels ROS-Glo H2O2 Assay kit (Promega, Madison, WI, USA #G8820) was used to measure intracellular ROS levels. HER2-positive breast cancer cells AU565 (1 × 104 cells/well),

(49) A single dose of AC102 restores hearing in a guinea pig model of noise-induced hearing loss to almost prenoise levelsProceedings of the National Academy of SciencesApril 9, 2024Hans Rommelspacher, Sujoy Bera, Benedikt Brommer, Rachael Ward, Monika Kwiatkowska, Tomasz Zygmunt, Florian Theden, Berk Üsekes, Neriman Eren, Michael Nieratschker, et al.AC102 or with DMTU for 2 h and levels of H2O2 was determined by luminescence-based assay (ROS-Glo, G8820, Promega, Madison, WI) and normalized to protein levels. For ATP measurement, HT22 cells were treated

(50) Cis-regulatory polymorphism at fiz ecdysone oxidase contributes to polygenic evolutionary response to malnutrition in DrosophilaPLoS GenetMarch 7, 2024Fanny Cavigliasso, Mikhail Savitsky, Alexey Koval, Berra Erkosar, Loriane Savary, Hector Gallart-Ayala, Julijana Ivanisevic, Vladimir L. Katanaev, Tadeusz J. Kaweckipotential substrate. For the first method, the H2O2 production was quantified with ROS-Glo H2O2 assay kit (#G8820, Promega, Switzerland). We incubated 28 μg of purified Fiz with 20 μL of 125 μM ecdysone or 20E and

(51) CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1α-JNK pathwaysExperimental & Molecular MedicineJanuary 22, 2024Joo-Young Im, Soo Jin Kim, Jong-Lyul Park, Tae-Hee Han, Woo-il Kim, Inhyub Kim, Bomin Ko, So-Young Chun, Mi-Jung Kang, Bo-Kyung Kim, et al.measurement H2O2 was measured using the ROS-Glo H2O2 assay according to the manufacturer’s protocol (Promega, G8820). Cells were plated into a 96-well plate (Thermo Scientific, 136101) at a density of 10,000 cells/well

(52) (1)H-NMR metabolomics analysis identifies hypoxanthine as a novel metastasis-associated metabolite in breast cancerScientific reportsJanuary 2, 2024Shakartalla SB, Ashmawy NS, Semreen MH, Fayed B, Al Shareef ZM, Jayakumar MN, Ibrahim S, Rahmani M, Hamdy R, Soliman SSM.xanthine/hypoxanthine assay kit (# ab155900) were purchased from Abcam (Cambridge, UK). ROS-Glo™ H2O2 Assay kit (# G8820) was purchased from Promega (Madison, USA). Pierce BCA protein assay kit (# 23227), Snail polyclonal

(53) Augmented Concentration of Isopentyl-Deoxynyboquinone in Tumors Selectively Kills NAD(P)H Quinone Oxidoreductase 1-Positive Cancer Cells through Programmed Necrotic and Apoptotic MechanismsCancers (Basel)December 14, 2023Jiangwei Wang, Xiaolin Su, Lingxiang Jiang, Matthew W. Boudreau, Lindsay E. Chatkewitz, Jessica A. Kilgore, Kashif Rafiq Zahid, Noelle S. Williams, Yaomin Chen, Shaohui Liu, et al.respective standard chemical assays according to the manufacturer’s protocols (Promega G7572, Promega G8820, and Promega G9071) [21]. 2.8. Immunofluorescence Assay To assess the possibility of double-strand

(54) Evaluating Manganese, Zinc, and Copper Metal Toxicity on SH-SY5Y Cells in Establishing an Idiopathic Parkinson's Disease ModelInternational journal of molecular sciencesNovember 9, 2023Pradhan SH, Liu JY, Sayes CM.Oxidative Stress Oxidative stress was measured using the Luminescent ROS-Glo H2O2 assay (Promega, #G8820). This assay measures the production of H2O2, the reactive species with the most prolonged half-life

(55) A novel triptolide analog downregulates NF-κB and induces mitochondrial apoptosis pathways in human pancreatic cancereLifeOctober 25, 2023Qiaomu Tian, Peng Zhang, Yihan Wang, Youhui Si, Dengping Yin, Christopher R Weber, Melissa L Fishel, Karen E Pollok, Bo Qiu, Fei Xiao, et al.kit,ACK lysing buffer,Quality Biological,118-156-101, Commercial assay or kit,ROS-Glo H2O2 assay,Promega,G8820, Commercial assay or kit,NuPAGE 10% Bis-Tris gel,Invitrogen,NP0301BOX, Commercial assay or kit,NuPAGE

(56) Development of novel cytoprotective small compounds inhibiting mitochondria-dependent cell deathiScienceOctober 20, 2023Mieko Matsuyama, Joseph T. Ortega, Yuri Fedorov, Jonah Scott-McKean, Jeannie Muller-Greven, Matthias Buck, Drew Adams, Beata Jastrzebska, William Greenlee, Shigemi Matsuyamaeffects of CSMs on OXPHOS and glycolysis were analyzed. ROS measurement ROS-Glo-H2O2 Assay kit (Promega G8820) was used to measure ROS in MEFs culture (medium and cells) according to the manufacturer’s protocol.

(57) Antioxidants stimulate BACH1-dependent tumor angiogenesisThe Journal of clinical investigationOctober 16, 2023Wang T, Dong Y, Huang Z, Zhang G, Zhao Y, Yao H, Hu J, Tüksammel E, Cai H, Liang N, et al.and seeded in white 96-well plates (5,000 cells/well). ROS were measured with the ROS-Glo-H2O2 assay (G8820, Promega). The ratio of GSH/GSSG was determined with the GSH/GSSG-Glo assay (V6611, Promega). Fluorescence

(58) Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo culturesSTAR protocolsSeptember 15, 2023Fietz A, Hurst J, Joachim SC, Schnichels S.commercial assays,Critical commercial assays ,, Promega’s ROS-Glo™ H2O2,"Promega, Walldorf, Germany",Cat#G8820 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, "GraphPad PRISM 9,

(59) P1357: MILESTONE (MODIFYING ELANE GOLDBERG–HOGNES BOX TO INHIBIT EXPRESSION), A UNIVERSAL, SAFE AND EFFECTIVE CRISPR/CAS9N-MEDIATED GENOME EDITING STRATEGY FOR ELANE RELATED SEVERE CONGENITAL NEUTROPENIAHemasphereAugust 8, 2023Masoud Nasri, Malte Ulrich Ritter, Perihan Mir, Benjamin Dannenmann, Masako Monika Kaufmann, Yun Xu, Natalia Borbaran-Bravo, Maksim Klimiankou, Claudia Lengerke, Cornelia Zeidler, et al.safety profiling. Reactive oxygen species (ROS) assay was measured by ROS-Glo Assay kit (Promega, #G8820). Assessment of phagocytosis kinetics was performed using the IncuCyte ZOOM system. Results: Using

(60) Mechanisms of metabolic stress induced cell death of human oligodendrocytes: relevance for progressive multiple sclerosisActa neuropathologica communicationsJuly 5, 2023Fernandes MGF, Mohammadnia A, Pernin F, Schmitz-Gielsdorf LE, Hodgins C, Cui QL, Yaqubi M, Blain M, Hall J, Dudley R, et al.ATP and H2O2 were measured using Cell Titer-Glo 2.0 (Promega, G9242) and ROS-Glo H2O2 Assay (Promega G8820). Normalization to cell number was calculated using the ratio between the measures of ATP and H2O2 obtained

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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