ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Evaluating Manganese, Zinc, and Copper Metal Toxicity on SH-SY5Y Cells in Establishing an Idiopathic Parkinson's Disease ModelInternational journal of molecular sciencesNovember 9, 2023Pradhan SH, Liu JY, Sayes CM.Oxidative Stress Oxidative stress was measured using the Luminescent ROS-Glo H2O2 assay (Promega, #G8820). This assay measures the production of H2O2, the reactive species with the most prolonged half-life

(62) A novel triptolide analog downregulates NF-κB and induces mitochondrial apoptosis pathways in human pancreatic cancereLifeOctober 25, 2023Qiaomu Tian, Peng Zhang, Yihan Wang, Youhui Si, Dengping Yin, Christopher R Weber, Melissa L Fishel, Karen E Pollok, Bo Qiu, Fei Xiao, et al.kit,ACK lysing buffer,Quality Biological,118-156-101, Commercial assay or kit,ROS-Glo H2O2 assay,Promega,G8820, Commercial assay or kit,NuPAGE 10% Bis-Tris gel,Invitrogen,NP0301BOX, Commercial assay or kit,NuPAGE

(63) A novel triptolide analog downregulates NF-κB and induces mitochondrial apoptosis pathways in human pancreatic cancerElifeOctober 25, 2023Qiaomu Tian, Peng Zhang, Yihan Wang, Youhui Si, Dengping Yin, Christopher R Weber, Melissa L Fishel, Karen E Pollok, Bo Qiu, Fei Xiao, et al.kit,ACK lysing buffer,Quality Biological,118-156-101, Commercial assay or kit,ROS-Glo H2O2 assay,Promega,G8820, Commercial assay or kit,NuPAGE 10% Bis-Tris gel,Invitrogen,NP0301BOX, Commercial assay or kit,NuPAGE

(64) Development of novel cytoprotective small compounds inhibiting mitochondria-dependent cell deathiScienceOctober 20, 2023Mieko Matsuyama, Joseph T. Ortega, Yuri Fedorov, Jonah Scott-McKean, Jeannie Muller-Greven, Matthias Buck, Drew Adams, Beata Jastrzebska, William Greenlee, Shigemi Matsuyamaeffects of CSMs on OXPHOS and glycolysis were analyzed. ROS measurement ROS-Glo-H2O2 Assay kit (Promega G8820) was used to measure ROS in MEFs culture (medium and cells) according to the manufacturer’s protocol.

(65) Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo culturesSTAR protocolsSeptember 15, 2023Fietz A, Hurst J, Joachim SC, Schnichels S.commercial assays,Critical commercial assays ,, Promega’s ROS-Glo™ H2O2,"Promega, Walldorf, Germany",Cat#G8820 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, "GraphPad PRISM 9,

(66) P1357: MILESTONE (MODIFYING ELANE GOLDBERG–HOGNES BOX TO INHIBIT EXPRESSION), A UNIVERSAL, SAFE AND EFFECTIVE CRISPR/CAS9N-MEDIATED GENOME EDITING STRATEGY FOR ELANE RELATED SEVERE CONGENITAL NEUTROPENIAHemasphereAugust 8, 2023Masoud Nasri, Malte Ulrich Ritter, Perihan Mir, Benjamin Dannenmann, Masako Monika Kaufmann, Yun Xu, Natalia Borbaran-Bravo, Maksim Klimiankou, Claudia Lengerke, Cornelia Zeidler, et al.safety profiling. Reactive oxygen species (ROS) assay was measured by ROS-Glo Assay kit (Promega, #G8820). Assessment of phagocytosis kinetics was performed using the IncuCyte ZOOM system. Results: Using

(67) P1357: MILESTONE (MODIFYING ELANE GOLDBERG–HOGNES BOX TO INHIBIT EXPRESSION), A UNIVERSAL, SAFE AND EFFECTIVE CRISPR/CAS9N-MEDIATED GENOME EDITING STRATEGY FOR ELANE RELATED SEVERE CONGENITAL NEUTROPENIAHemasphereAugust 8, 2023Masoud Nasri, Malte Ulrich Ritter, Perihan Mir, Benjamin Dannenmann, Masako Monika Kaufmann, Yun Xu, Natalia Borbaran-Bravo, Maksim Klimiankou, Claudia Lengerke, Cornelia Zeidler, et al.safety profiling. Reactive oxygen species (ROS) assay was measured by ROS-Glo Assay kit (Promega, #G8820). Assessment of phagocytosis kinetics was performed using the IncuCyte ZOOM system. Results: Using

(68) Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscleeLifeJune 2, 2023Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.measured for oxidative stress using the ROS-Glo H2O2 assay according to the manufacturer’s instructions (G8820, Promega). Luminescence was measured using a Tecan Spark M10 microplate reader (Tecan, Männedorf, Switzerland).

(69) Endoplasmic reticulum stress in the intestinal epithelium initiates purine metabolite synthesis and promotes Th17 cell differentiation in the gutImmunityMay 9, 2023Duan J, Matute JD, Unger LW, Hanley T, Schnell A, Lin X, Krupka N, Griebel P, Lambden C, Sit B, et al.,Miltenyi Biotec,Cat# 130-106-643 AmplexTM UltraRed,Invitrogen,Cat# A22188 ROS-Glo™ H2O2 Assay,Promega,Cat# G8820 RNeasy Plus Micro Kit,Qiagen,Cat# 74034 RNeasy Plus Mini Kit,Qiagen,Cat# 74134 Superscript™ IV VILO™ Master Mix

(70) Combating castration-resistant prostate cancer by co-targeting the epigenetic regulators EZH2 and HDACPLOS BiologyApril 27, 2023Amy E. Schade, Ryan Kuzmickas, Carrie L. Rodriguez, Kaia Mattioli, Miriam Enos, Alycia Gardner, Karen CichowskiTable. Reactive oxygen species assays ROS levels were detected using the ROSglo Assay Kit (Promega, cat # G8820) after 24 hours of combination treatment. ROS levels were normalized to DMSO-treated controls. ATP

(71) Treatment with Exogenously Added Catalase Alters CD8 T Cell Memory Differentiation and FunctionAdv Biol (Weinh)April 1, 2023Aksoylar HI, Patsoukis N.hydrogen peroxide concentration in the culture supernatant we used the ROS-Glo™ H2O2 assay kit (Promega, G8820) according to the manufacturer’s instructions. Briefly, 40 μl of culture supernatant, were mixed with

(72) PPAR-γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1α/Δψ m Pathways by Inhibiting PyroptosisPPAR ResearchMarch 27, 2023Zhencheng Feng, Qiuxiang Huang, Xingliang Zhang, Pengfei Xu, Siming Li, Dongli Ma, Qingqi MengFisher Scientific) for detecting polyvinylidene fluoride (PVDF) membranes. ROS-GloTM H2O2 Assay kit (G8820, Promega). Nrf2 small interfering RNA (siRNA) (sense: GCUCAGAACUGUAGGAAAATT; antisense: UUUUCCUACAGUUCUGAGCTT)

(73) Dual Role of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 in Inflammasome and Pancreatic β-Cell FunctionInternational Journal of Molecular SciencesMarch 5, 2023Rania Saeed, Abdul Khader Mohammed, Sarra E. Saleh, Mohammad M. Aboulwafa, Khaled M. Aboshanab, Jalal Taneerainstructions, the intracellular generation of ROS was detected using a ROS-Glo H2O2 assay kit (Cat #G8820, Promega, Madison, WI, USA). Briefly, the transfected and non-transfected INS-1 cells were treated with

(74) Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin SecretionMetabolitesFebruary 20, 2023Saeed R, Mohammed AK, Saleh SE, Aboshanab KM, Aboulwafa MM, Taneera J.Oxygen Species (ROS) Measurements Intracellular ROS was detected using a ROS-Glo H2O2 assay kit (Cat #G8820, Promega, Madison, WI, USA). In brief, 48 h post-transfection, INS-1 cells were incubated with H2O2

(75) Liver X Receptor Inverse Agonist GAC0001E5 Impedes Glutaminolysis and Disrupts Redox Homeostasis in Breast Cancer CellsBiomoleculesFebruary 10, 2023Premaratne A, Ho C, Basu S, Khan AF, Bawa-Khalfe T, Lin CY.(Corning Inc., Corning, NY, USA, #4517). Freshly prepared 50 μL of ROS-GloTM (Promega, Madison, WI, USA, #G8820) solution was added and incubated for 20 min. After 20 min, luminescence was read using the PerkinElmer

(76) Mechanism of cis-Nerolidol-Induced Bladder Carcinoma Cell DeathCancersFebruary 3, 2023Glumac M, Čikeš Čulić V, Marinović-Terzić I, Radan M.(H2O2-Glo) Intracellular ROS levels were measured using the ROS-Glo H2O2 assay (Promega Corp., Madison, WI, USA, G8820) following the manufacturer’s instructions. Briefly, TCCSUP cells were treated with nerolidol (50 mg/L)

(77) Loss of LKB1-NUAK1 signalling enhances NF-κB activity in a spheroid model of high-grade serous ovarian cancerScientific ReportsDecember 1, 2022Adrian Buensuceso, Jamie Lee Fritz, Olga Collins, Yudith Ramos Valdés, Matthew J. Borrelli, Gabriel E. DiMattia, Trevor G. ShepherdCayman Chemical (cat. 10010266; Ann Arbor, MI, USA). ROS-Glo H2O2 Assay was purchased from Promega (cat. G8820). Generation of STK11KO and NUAK1KO cell lines Generation of OVCAR8-STK11KO16 and OVCAR8-NUAK1KO20

(78) Efflux-linked accelerated evolution of antibiotic resistance at a population edgeMolecular CellNovember 9, 2022Souvik Bhattacharyya, Madhumita Bhattacharyya, Dylan M. Pfannenstiel, Anjan K. Nandi, YuneSahng Hwang, Khang Ho, Rasika M. HarsheyA1120 Measure-IT™ High-Sensitivity Nitrite Assay Kit,Thermo,Cat# M36051 ROS-Glo™ H2O2 Assay,Promega,Cat# G8820 LIVE/DEAD™ BacLight™ Bacterial Viability Kit,Thermo,Cat# L7007 CellROX™ Green Reagent,Thermo,Cat#

(79) Inhibition of autophagy with chloroquine dysregulates mitochondrial quality control and energetics in adipocytesArchives of pharmacal researchOctober 1, 2022Javaid HMA, Lim H, Shin S, Huh JY.measurement Hydrogen peroxide was measured using a commercially available ROS-Glo H2O2 assay kit (G8820, Promega; Madison, WI, USA) in accordance with the manufacturer’s instructions. Briefly, after the cells

(80) The Anti-Proliferative Lichen-Compound Protolichesterinic Acid Inhibits Oxidative Phosphorylation and Is Processed via the Mercapturic Pathway in Cancer CellsPlanta MedicaSeptember 1, 2022Freyr JÃ³hannsson, Paulina Cherek, Maonian Xu, Ã“ttar Rolfsson, Helga M. Ã–gmundsdÃ³ttirNADPH] and determine their ratio. H 2 O 2 was measured by using the ROS-Glo H 2 O 2 Assay kit (cat. no. G8820; Promega) according to the manufacturerʼs instructions. H 2 O 2 substrate solution was added for 2 h

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