ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK SignalingFASEB JJuly 30, 2026Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr CzekajDetermination of Oxidative Stress Parameters 2.9.1 ROS‐Glo H2O2 Assay The ROS‐Glo H2O2 Assay (G8820; Promega, Madison, WI, USA) was used to measure hydrogen peroxide (H2O2) levels. Cells were seeded at

(2) The characterization of a new stabilized lactonase from the endophytic fungi Aspergillus pseudonomiae with anti-bacterial and anti-fungal propertiesProtein SciJuly 28, 2026Dor Ben Tovim, Olena Trofimyuk, Elon Yariv, Gali Prag, Livnat Afriat-Jurnoupermeable ROS, directly in fungi culture, regardless of its source, we used ROS‐Glo™ H2O2 kit (Promega, G8820) according to the manufacturer's protocol. Briefly, aliquots of P. expansum suspension were added to

(3) Preclinical characterization and phase 1 clinical testing of targeting mitochondrial peroxiredoxin 3 in cancerNat CommunJuly 14, 2026Victoria Gibson, Joanna Dzialo, Terri Messier, Aleksandra Bzura, Charlotte Poile, Jan Rogel, Jens C. Hahne, Aida Habibovic, Stephanie Stead, Alexis Saaman, et al.found in Supplementary Table 5. Compound treatment and ROS detection The ROS-Glo H2O2 assay (Cat# G8820, Promega, Walldorf, Germany) was used to measure the level of H2O2 in culture after treatment with TS

(4) Ferroptosis and Wnt/β-Catenin Signaling Triggered by Environmentally Relevant Nanoscale Polypropylene Plastics in Human Intestinal ModelsACS Environmental AuMay 20, 2026Sadman Sakib, Maohui Chen, Stephanie Skerrett, Daniel Prezgot, Magda Vandenberg, Adrian F. Pegoraro, Zygmunt J. Jakubek, Zeina Maan, Shan ZouLevel Measurements The reactive oxygen species (ROS) levels were determined using ROS-Glo H2O2 Assay (G8820, Promega). Similar to the viability assays, HIEC-6 cells (5000 cells/well) and HSIOs (50 organoids/well)

(5) High-intensity irreversible electroporation targeting intracellular structures enhance tumor ablation in lung cancer modelsPLoS OneApril 22, 2026Hong Bae Kim, Jin Young Youm, Joon-Mo Yang, Sung Bo Simattachment and recovery. The culture media were removed from all wells, and ROS-Glo™ H2O2 substrate (Promega G8820) was added, with the plate incubated for 2 h at 37 °C in a CO2 incubator to allow substrate interaction

(6) Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancerNat CommunApril 15, 2026Yapeng Su, Chunmei Liu, Xiang Lu, Hui-Yu Chuang, Guideng Li, Shiqun Shao, Yan Kong, Jihoon W. Lee, Rachel H. Ng, Stephanie Wong, et al.Cell number normalized ROS levels were quantified using the ROS-Glo™ H2O2 assay kit (Promega, Cat# G8820) following the manufacturer’s protocol. Luminescence signals were measured on a Synergy H4 Plate Reader. RNA-seq

(7) Photobiomodulation restores blood–brain barrier integrity after hypoxia via endothelial von Willebrand factor modulation in a humanised tricellular transwell modelJ PhysiolApril 11, 2026Mirriam Domocos, Denis E. Bragin, Nagesh C. Shanbhag, Luise Schlotterose, Mootaz M. Salmanand the pellet was subjected to further qPCR analysis. ROS‐Glo H2O2 assay The ROS‐Glo H2O2 assay (G8820; Promega, Madison, WI, USA) was used to analyse the hydrogen peroxide (H2O2) production for normoxia

(8) Differential Effects of Curcumin and Cordycepin on Oral Squamous Cell Carcinoma Cells: ROS-Mediated Cytotoxicity and Real-Time Morphological AnalysisMoleculesApril 7, 2026Bianca Voicu Balasea, Miruna-Silvia Stan, Radu Radulescu, Ana Cernega, Kersti Alm, Monica Musteanu, Florentina Rus, Alexandra Ripszky, Silviu Mirel Pituruoxygen species, was measured using the ROS-Glo™ H2O2 Assay Kit (Promega, Madison, WI, USA; Cat. No. G8820) in HGEpiC and OECM-1 cells following treatment. The ROS-Glo™ reagent was added to the culture medium

(9) Targeting ubiquitin signaling vulnerabilities in KEAP1-inactivated lung cancerThe EMBO JournalMarch 20, 2026Varun Jayeshkumar Shah, Oliver Hartmann, Martin Wegner, Cristian Prieto-Garcia, Rubina Kazi, Viktoria von Heyl zu Herrnsheim, Amin Wanli, Igor Mačinković, Bianka Bohnacker, Koraljka Husnjak, et al.software,v.0.6.0,https://qupath.github.io/ ImageJ,2.17.0,https://imagej.net/ij/download.html Other,Other,Other CellTiter-Glo® Luminescent Cell Viability Assay,Promega,G7570 ROS-Glo™ H₂O₂ Assay,Promega,G8820 NADP/NADPH-Glo™ Assay,Promega,G9081 NAD/NADH-Glo™ Assay,Promega,G9071 Seahorse XFe96/XF Pro FluxPak

(10) Differential Effects of Apigenin on Normal and Squamous Oral Epithelial Cells Reveal Redox–Autophagy Signaling Vulnerabilities in OSCCInternational Journal of Molecular SciencesFebruary 24, 2026Bianca Voicu Balasea, Miruna-Silvia Stan, Miruna Dinescu, Marina Imre, Radu Radulescu, Ana Cernega, Monica Musteanu, Alexandra Ripszky, Silviu Mirel Pituruoxygen species, was determined using the ROS-Glo™ H2O2 Assay Kit (Promega, Madison, WI, USA; Cat. No. G8820) after treatment with apigenin in the two oral cell lines: normal and tumoral. After 24 and 48 h of

(11) Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profilesCell Reports MethodsFebruary 23, 2026Marie Sejberg Øhlenschlæger, Pia Jensen, Jesper Foged Havelund, Sissel Ida Schmidt, Fadumo Abdullahi Mohamed, Magdalena Sutcliffe, Sofie Blomberg Elmkvist, Lucrezia Criscuolo, Steven W. Wingett, Ilaria Chiaradia, et al.assays,Critical commercial assays ,, LDH-Glo™ Cytotoxicity Assay,Promega,J2380 ROS-Glo® H2O2 Assay,Promega,G8820 Seahorse XF Real-Time ATP Rate Assay Starter Pack,Agilent,103677–100 Seahorse XFe96 FluxPak,Agilent,102601–100 P3

(12) In Vitro Investigations on the Antioxidant Effects of Vitamin D in a Panel of Cancer Cell LinesBiomedicinesFebruary 20, 2026Lina Elsalem, Farah A. Shobaki, Nosayba Al-Azzam, Abrar A. Aleikish, Haneen A. Basheeron ROS production was evaluated using the ROS-Glo™ H2O2 assay (Promega, Madison, WI, USA, Cat. No. G8820) according to the manufacturer’s instructions. Cells were seeded at a density of 8.5 × 104 cells per

(13) Molecular and Cellular Determinants of Human Iron Overload CardiomyopathybioRxiv : the preprint server for biologyFebruary 4, 2026Modak SS, Greenberg L, Stump WT, Greenberg AE, Huebsch N, Greenberg MJ.levels were measured using the ROS-Glo H2O2 kit according to manufacturer’s instructions (Promega, Cat # G8820). 80,000 cells/well in RPMI with insulin medium were seeded into 96 well plates. On the day of the assay,

(14) Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effectsGenome BiologyJanuary 31, 2026Majid Pahlevan Kakhki, Fatemeh Rangani, Ewoud Ewing, Chiara Starvaggi Cucuzza, Galina Zheleznyakova, Maria Kalomoiri, Lea Kenny, Anika Raghavan, Chandana Rao Prakash, Gabe van den Hoeven, et al.measurement Extracellular hydrogen peroxide (H₂O₂) levels were quantified using the ROS-Glo™ H₂O₂ Assay Kit (Promega, Cat. #G8820) according to the manufacturer’s protocol. A 10 mM H₂O₂ substrate stock was diluted in H₂O₂ Substrate

(15) Hyperbranched Polymer Dendrimers Embedded in Electrospun Nanofibers for Safe and Sustainable Antibacterial Filtration MaterialsPolymers (Basel)January 30, 2026Matej Buzgo, Baturalp Yalcinkaya, Miroslav Doupník, Radmila Žižková, Viktorie Rockova, Kristyna Vrbova, Michaela Sobotkova, Alena Milcova, Anezka Vimrova, Michal Šíma, et al.oxidative stress. Production of H2O2 in extracts was analysed using ROS-Glo™ H2O2 Assay (Promega, Cat. No. G8820, Madison, WJ, USA). 80 µL of the extract, the control medium (negative control), or the medium containing

(16) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.using a fluorescent microscope. ROS detection To quantify ROS, the ROS-Glo H2O2 Assay Kit (Promega #G8820) was used according to the manufacturer’s instructions. Briefly, 20 mg of tissue was lysed with Passive Lysis 5X Buffer

(17) Baicalin Alleviates Chronic Restraint Stress-Induced Depression-like Behavior by Suppressing ROS/H2O2 Generation via a BDNF-Associated Mechanism in MiceAntioxidants (Basel)January 21, 2026Yu-Ning Teng, Tien-Wei Hsu, Wei-Hao Peng, Cheng-Chun Wu, Tian-Huei Chu, Yung-Kuo Lee, Ming Tatt Lee, Yu-Cheng Hoexperiments were performed according to the ROS-Glo™ H2O2 Assay manual (Promega, Madison, WI, USA, Cat. #G8820). Briefly, cells were seeded into a 96-well white plate at a density of 1 × 105 cells per well and incubated

(18) RAS/PI3K pathway mutations sensitise epithelial ovarian cancer cells to a PARP/NAMPT inhibitor combinationCommun BiolDecember 19, 2025Michael Gruet, Yitao Xu, Lyutong An, Yurui Ma, Cristina Balcells, Katie Tyson, Laila C. Evangelista, Sarah Spear, Yuewei Xu, Flora McKinney, et al.drug treatment. After 24- (human) or 48-h (ID8), H2O2 was measured using ROS-Glo H2O2 assay (Promega, G8820) according to the manufacturer’s manual. Briefly, 6-h before the endpoint is reached, 20 μL of combined

(19) Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferationNat CommunDecember 13, 2025Aristeidis P. Sfakianos, Rebecca M. Raven, Tom Smith, Xiao-Ming Sun, Thomas E. Mulroney, Mariavittoria Pizzinga, Veronica Dezi, Angela Rubio Tenor, Mark Stoneley, Cameron H. Cole, et al.described previously. ROS detection assay For ROS detection we used ROS-Glo™ H2O2 Assay (Promega, G8820), according to manufacturer’s protocol. Menadione (Supplementary Table 1) was used as a positive control. ATP

(20) Targeted Inhibition of ELANE Expression Using Adenine Base Editing to Treat Severe Congenital NeutropeniaMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Betül Findik, Benjamin Dannenmann, Franka Bernhard, Masako Monika Kaufmann, Sandra Ammann, Sergey Kandabarau, Maksim Klimiankou, Fabian Mauch, Patrick Münzer, Oliver Borst, et al.a form of Reactive Oxygen Species (ROS), were measured using the ROS-Glo H2O2 assay kit (Promega, #G8820) on a GloMax multidetection system plate reader, following the manufacturer’s protocol. Phagocytosis

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