DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(161) Early maturation and hyperexcitability is a shared phenotype of cortical neurons derived from different ASD-associated mutationsTranslational PsychiatryJuly 6, 2023Yara Hussein, Utkarsh Tripathi, Ashwani Choudhary, Ritu Nayak, David Peles, Idan Rosh, Tatiana Rabinski, Jose Djamus, Gad David Vatine, Ronen Spiegel, et al.solution overnight at 4 °C. On the next day, they were washed in DPBS and incubated with DAPI (Abcam, ab228549, 1:1000) and corresponding secondary antibodies (Abcam, ab150084, ab150117, ab175711, 1:250) for 60

(162) Immunohistologic Features of Cerebral Venous Thrombosis Due to Vaccine-Induced Immune Thrombotic ThrombocytopeniaNeurology(R) neuroimmunology & neuroinflammationJuly 1, 2023Geeraerts T, Guilbeau-Frugier C, Garcia C, Memier V, Raposo N, Bonneville F, Gales C, Darcourt J, Voisin S, Ribes A, et al.temperature. In some instances, wheat germ agglutinin was used. Sections were then stained with DAPI (Abcam, ab228549) for 15 minutes. Sections were labeled with secondary antibodies (Oregon-Green-488 goat antirabbit IgG,

(163) Restoration of normal blood flow in atherosclerotic arteries promotes plaque stabilizationiScienceJune 16, 2023Schake MA, McCue IS, Curtis ET, Ripperda TJ Jr, Harvey S, Hackfort BT, Fitzwater A, Chatzizisis YS, Kievit FM, Pedrigi RM.mg),Apotex Corp.,Cat# 60505-2579-09 Prolong Gold antifade reagent,Invitrogen,Cat#P36930 DAPI,Abcam,Cat#ab228549 Hydrochloric Acid,Sigma-Aldrich,Cat#32031-500ML Ethanol,Decon Laboratories Inc.,Cat#2701 Acetone,Fisher

(164) Chitosomes Loaded with Docetaxel as a Promising Drug Delivery System to Laryngeal Cancer Cells: An In Vitro Cytotoxic StudyInternational Journal of Molecular SciencesJune 8, 2023Christian R. Moya-Garcia, Nicole Y. K. Li-Jessen, Maryam Tabrizian(cat. # ab65393), ALEXA647/EGFR (cat. # ab192982), ALEXA488/TUBIII (cat. # ab195879), and DAPI (cat. # ab228549) from Abcam (Cambridge, UK) were used to visualize the cell membrane, cytoskeleton, and nucleus, respectively.

(165) Enhancement of Collagen-I Levels in Human Gingival Fibroblasts by Small Molecule Activation of HIF-1αJournal of Agricultural and Food ChemistryMay 24, 2023Lucia Adriana Lifshits, Miryam Rabin, Ran Tohar, Francesca Netti, Matan Gabay, Marina Sova, Daniel Z. Bar, Evgeny Weinberg, Lihi Adler-Abramovich, Maayan Gal(ab150077, Abcam)), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (ab150115, Abcam), and DAPI Staining Solution (ab228549, Abcam). Cellular viability was assessed using a live/dead viability assay (04511, Merck) and CellTiter

(166) Zika virus triggers autophagy to exploit host lipid metabolism and drive viral replicationCell Communication and SignalingMay 19, 2023Gloria Stoyanova, Sidra Jabeen, Joselyn Landazuri Vinueza, Sounak Ghosh Roy, Richard A. Lockshin, Zahra Zakeriwashed with 1X PBS for 5 min and then stained with 4, 6 -diamidino-2-phenylindole (DAPI) (1 mM) (# ab228549, ABCAM) for 8 min. Cells were washed twice with 1 × PBS, mounted, and embedded in Fluoromount® and observed

(167) High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seqNature CommunicationsMay 12, 2023Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, et al.aliquot of nuclei was stained with DAPI (4′,6-diamidino-2-phenylindole) staining solution (Abcam, Cat # ab228549), loaded on a hemocytometer and observed under an inverted fluorescence microscope (Nikon Eclipse, Ts2-FL).

(168) Advanced cell-based products generated via automated and manual manufacturing platforms under the quality by design principle: Are they equivalent or different?HeliyonMay 3, 2023Duc M. Hoang, Quyen T. Nguyen, Trang T.K. Phan, Anh T.L. Ngo, Phuong T. Pham, Trung Q. Bach, Phuong T.T. Le, Hoa T.P. Bui, Liem Nguyen Thanhwere fixed with 4% paraformaldehyde (PFA) and stained with DAPI staining solution (1:5000, Abcam, #ab228549) after the assay. The ImageXpress® Micro Confocal High-content Imaging System (Molecular Devices) was

(169) The mitochondrial Ca 2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationbioRxivMay 3, 2023Emily Fernández García, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Jason R. Pitarresi, Anil K. Rustgi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin FoskettScientific, R37174), according to manufacturer’s instructions. After 15-min incubation, 4 mM DAPI (Abcam, ab228549) was added to the suspension and cells were analyzed by FACS with emission filters 530 and 440 nm for

(170) Lipid Deposition Profiles Influence Foreign Body ResponsesAdvanced materials (Deerfield Beach, Fla.)May 1, 2023Schreib CC, Jarvis MI, Terlier T, Goell J, Mukherjee S, Doerfert MD, Wilson TA, Beauregard M, Martins KN, Lee J, et al.PBST for 3 washes (5 minutes per wash), the mounted slides were incubated with DAPI (1:1000; Abcam ab228549) in 1x PBST at room temperature for 5 minutes. After final washing steps with 1x PBST for 3 washes (5

(171) Selective inhibition of somatostatin-positive dentate hilar interneurons induces age-related cellular changes and cognitive dysfunctionPNAS NexusMay 1, 2023Lyu J, Nagarajan R, Kambali M, Wang M, Rudolph U.microscope. To verify viral placements and immunofluorescence labeling of mCherry, DAPI stain (Abcam, cat # ab228549) was added to free-floating 30-µm coronal sections using the concentration of 1 µm in the dark. The

(172) Acetaldehyde via CGRP receptor and TRPA1 in Schwann cells mediates ethanol-evoked periorbital mechanical allodynia in mice: relevance for migraineJournal of Biomedical ScienceApril 26, 2023Lorenzo Landini, Daniel Souza Monteiro de Araujo, Martina Chieca, Gaetano De Siena, Elisa Bellantoni, Pierangelo Geppetti, Romina Nassini, Francesco De Loguat RT. Slides were then coverslipped with mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, #ab228549, Abcam). Fluorescence images were obtained using an AxioImager 2 microscope (Carl Zeiss). The fluorescence

(173) Insulin Sensitization by PPARγ and GLUT-4 Overexpression/Translocation Mediates the Antidiabetic Effect of Plantago australis †Pharmaceuticals (Basel)April 3, 2023Samuel Estrada-Soto, Kathia Ornelas-Mendoza, Gabriel Navarrete-Vázquez, Fabiola Chávez-Silva, Julio Cesar Almanza-Pérez, Rafael Villalobos-Molina, Erandi Ortiz-Barragán, Hilda Loza-Rodríguez, Julio César Rivera-Leyva, Angélica Flores-Flores, et al.assembly of PBS/glycerol/DAPI (Inorganic Polyphosphate Stores by 4′,6-diamidino-2-phenyl-indole) (Abcam, ab228549) was used as a staining reagent for DNA detection and nuclear integrity. Cell images were taken with

(174) Label-free macrophage phenotype classification using machine learning methodsScientific reportsMarch 30, 2023Hourani T, Perez-Gonzalez A, Khoshmanesh K, Luwor R, Achuthan AA, Baratchi S, O'Brien-Simpson NM, Al-Hourani A.#PKH67GL) as per the manufacturer instructions, and cell nuclei were stained with 0.5 μg/mL DAPI (Abcam, # ab228549) for 5 min at room temperature. Cells were washed again with PBS and the immunofluorescent pictures

(175) TNF-α-Mediated Endothelial Cell Apoptosis Is Rescued by Hydrogen SulfideAntioxidants (Basel)March 16, 2023Lorena Diaz Sanchez, Lissette Sanchez-Aranguren, Keqing Wang, Corinne M. Spickett, Helen R. Griffiths, Irundika H. K. DiasAfter treatments, fresh medium containing 2.5 µM SF7-AM was added and incubated at 37 °C for 30 min. DAPI (#ab228549, Abcam, Cambridge, UK), as a counterstain for nuclear morphology, was incubated at 37 °C for 10 min.

(176) Emodin alleviates lung ischemia–reperfusion injury by suppressing gasdermin D‐mediated pyroptosis in ratsThe Clinical Respiratory JournalMarch 1, 2023Tao Jin, Fen Ai, Jin Zhou, Lin Kong, Zhangming Xiong, Dingping Wang, Ruilin Lu, Zhen Chen, Muxi Zhangwith secondary antibody (Alexa Fluor 488; Beyotime) for 1 h at room temperature in the dark. DAPI (ab228549; Abcam) was used for nuclear staining. The staining pattern was imaged using a fluorescence microscope

(177) A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensingJournal of cell scienceMarch 1, 2023Di Pizio A, Marvaldi L, Birling MC, Okladnikov N, Dupuis L, Fainzilber M, Rishal I.donkey anti-rabbit-IgG conjugated to Alexa Fluor 647 (1:1000; Jackson ImmunoResearch) and DAPI (Abcam, ab228549, 1:5000). After three washes in 1× PBS, coverslips were rinsed in ddH2O and mounted with Fluoromount-GTM. Immunohistochemistry

(178) Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissuesAPL BioengineeringMarch 1, 2023Jiawen Chen, Seyed Mohammad Mir, Maria R. Hudock, Meghan R. Pinezich, Panpan Chen, Matthew Bacchetta, Gordana Vunjak-Novakovic, Jinho Kimantibody, the tissues samples were incubated with DAPI solution (4′,6-diamidino-2-phenylindole; Cat. No. ab228549, Abcam) for 10 min to stain cell nuclei. Finally, the stained tissues were placed onto a glass slide

(179) Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in HypoxiaTranslational Vision Science & TechnologyFebruary 10, 2023Victoria Messerschmidt, Wen Ren, Michael Tsipursky, Joseph Irudayarajcontrol and cells treated with TACS-nuclease were treated as positive controls. DAPI staining solution (ab228549, Abcam, Cambridge, UK) was used to stain the nuclei following the manufacturer's instructions. DAPI

(180) p15INK4B is an alternative marker of senescent tumor cells in colorectal cancerHeliyonFebruary 1, 2023Soon Sang Park, Young-Kyoung Lee, So Hyun Park, Su Bin Lim, Yong Won Choi, Jun Sang Shin, Young Hwa Kim, Jang-Hee Kim, Tae Jun Parkstabilization (48 h). To check heterochromatin foci in senescence models, 1:10,000 diluted DAPI solution (ab228549, Abcam, Cambridge, UK) was stained and cells were observed under fluorescence microscopy (AXIO Imager.M1,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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