DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(181) Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanomaNature CommunicationsDecember 1, 2022Claudia Capparelli et al.secondary antibody, Phalloidin-TRITC (Sigma, P1951, 1:500) to visualize the cytoplasm, and DAPI (Abcam, ab228549, 1:1000) to visualize nuclei. Coverslips were mounted and imaged using a 40X objective lens on a Nikon

(182) Spatially resolved proteomics via tissue expansionNature CommunicationsNovember 30, 2022Lu Li, Cuiji Sun, Yaoting Sun, Zhen Dong, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, et al.23 °C. The sections were then washed three times with PBS and counterstained with DAPI (5 μM in PBS; ab228549; Abcam, US), before being mounted with Prolong Gold antifade reagent (ThermoFisher; US). Tissue expansion

(183) Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue ConstructsACS Applied Materials & InterfacesNovember 23, 2022Shabir Hassan, Eduardo Gomez-Reyes, Eduardo Enciso-Martinez, Kun Shi, Jorge Gonzalez Campos, Oscar Yael Perez Soria, Eder Luna-Cerón, Myung Chul Lee, Isaac Garcia-Reyes, Joshua Steakelum, et al.Millipore Sigma. Primary antibodies for VE-cadherin (ab33168), MyHC (ab1242015), F-actin (ab205), and DAPI (ab228549) were purchased from Abcam (Cambridge, U.K.) and used according to the manufacturer’s protocol. PECAM-1

(184) Salidroside attenuates HALI via IL-17A-mediated ferroptosis of alveolar epithelial cells by regulating Act1-TRAF6-p38 MAPK pathwayCell communication and signaling : CCSNovember 21, 2022Guo B, Zuo Z, Di X, Huang Y, Gong G, Xu B, Wang L, Zhang X, Liang Z, Hou Y, et al.antibody (E032620, 1:400, Earthox) for 2 h at room temperature. Counterstaining of nuclei with DAPI (ab228549, Abcam) was also performed. Then slides were observed, and the images were captured with a fluorescence microscope

(185) Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortexActa neuropathologica communicationsOctober 29, 2022Laszlo ZI, Hindley N, Sanchez Avila A, Kline RA, Eaton SL, Lamont DJ, Smith C, Spires-Jones TL, Wishart TM, Henstridge CM.1:50 donkey anti-rabbit Alexa fluor 594–ab150076, Abcam, respectively) for 30 min and DAPI (1:1000, ab228549, Abcam) for 5 min. Samples were then mounted onto slides with Immumount mounting media. Images were

(186) Underwater instant adhesion mechanism of self-assembled amphiphilic hemostatic granular hydrogel from Andrias davidianus skin secretioniScienceOctober 21, 2022Yuqing Liu, Yinghao Li, Haitao Shang, Wen Zhong, Quan Wang, Kibret Mequanint, Chuhong Zhu, Malcolm Xing, Hong WeiFluor 568-conjugated secondary antibodies,Abcam,"Abcam Cat# ab175471, RRID:AB_2576207" DAPI,Abcam,ab228549 "Sprague Dawley (SD) rats, 6-week-old",The Animal Experimental Center of Third Military Medical University,N/A ,, "Chemicals,

(187) Small molecule SJ572946 activates BAK to initiate apoptosisiScienceOctober 21, 2022Sekar G, Singh G, Qin X, Guibao CD, Schwam B, Inde Z, Grace CR, Zhang W, Slavish PJ, Lin W, et al.HY-15141 AlexaFluor488-conjugated Annexin V,Abcam,Cat# ab219916 Propidium iodide,Abcam,Cat# ab14083 DAPI,Abcam,Cat# ab228549 AlexaFluor488-conjugated anti-cytochrome c antibody clone 6H2.B4,BioLegend,Cat# 612310 BMH crosslinker,Thermo Fisher Scientific

(188) Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic MiceeneuroOctober 20, 2022Pei He, Binjun He, Shu Li, Wen Chai, Wei Rao, Yu Zhu, Wenzhi Chen, Ping Zhang, Xiong Zhang, Haili Pan, et al.IgM/IgG H&L 1:100 (A10680, Life Tech Ltd.) conjugated to Alexa Fluor 488 for 2 h at RT and DAPI (Blue; ab228549, Abcam, Hong Kong Ltd.). The sections were then stained, extensively washed, mounted with antifade medium,

(189) Electrical Stimulation of Human Adipose-Derived Mesenchymal Stem Cells on O2 Plasma-Treated ITO Glass Promotes Osteogenic DifferentiationInternational Journal of Molecular SciencesOctober 18, 2022Seungho Baek, Heekyung Park, Fatma Dilara Igci, Donghyun Lee(Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), ab150113 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647), ab150083) and DAPI staining solution (ab228549) used in immunohistochemistry experiments were purchased from Abcam (Cambridge, UK). 3.2. Experimental

(190) Polydatin Ameliorates High Fructose-Induced Podocyte Oxidative Stress via Suppressing HIF-1α/NOX4 PathwayPharmaceuticsOctober 16, 2022Ding H, Tang C, Wang W, Pan Y, Jiao R, Kong L.Gaithersburg, MD, USA) for 1 h at room temperature, and then stained with DAPI (4’,6-diamidino-2-phenylindole, ab228549, 1:2000, Abcam, Cambridge, MA, USA), at room temperature for 10 min. Finally, the sections were examined

(191) GPC3-Unc5 receptor complex structure and role in cell migrationCellOctober 13, 2022Akkermans O, Delloye-Bourgeois C, Peregrina C, Carrasquero-Ordaz M, Kokolaki M, Berbeira-Santana M, Chavent M, Reynaud F, Raj R, Agirre J, et al.,Fisher Scientific,Cat#12891660 Fetal Bovine Serum (FBS),GIBCO,Cat#10437028 Dapi Staining Solution,Abcam,Cat#ab228549 High Capacity Streptavidin Agarose Resin,Thermo Fisher Scientific,Cat#10733315 "Magnesium Sulfate,

(192) BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiencyNature communicationsOctober 5, 2022Wang F, Liu Y, Ni F, Jin J, Wu Y, Huang Y, Ye X, Shen X, Ying Y, Chen J, et al.oocytes were incubated for 1 h at room temperature with secondary antibodies (Thermo Fisher) and DAPI (ab228549, Abcam, U.K.). Negative controls included parallel oocytes that were treated without primary antibodies.

(193) SMPDL3b modulates radiation-induced DNA damage response in renal podocytesFASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 1, 2022Francis M, Ahmad A, Bodgi L, Azzam P, Youssef T, Abou Daher A, Eid AA, Fornoni A, Pollack A, Marples B, et al.BSA in TBS-T. After three wash with TBS-T, sections were stained with DAPI Staining Solution (cat # ab228549, Abcam, USA) for 30 min. Sections were washed three times with TBS with mild agitation. Finally, sections

(194) A Nesprin-4/kinesin-1 cargo model for nuclear positioning in cochlear outer hair cellsFrontiers in Cell and Developmental BiologySeptember 23, 2022Shahar Taiber, Oren Gozlan, Roie Cohen, Leonardo R. Andrade, Ellen F. Gregory, Daniel A. Starr, Yehu Moran, Rebecca Hipp, Matthew W. Kelley, Uri Manor, et al.myosin VIIa (Proteus Biosciences, 25-6790) 1:250, rabbit anti-FLAG (Abcam, ab205606) 1:100, DAPI (Abcam ab228549) 1:1,000, and goat anti-rabbit Alexa Fluor 488 (Cell Signaling 4412s) 1:250. Image analysis All data

(195) Tackling recalcitrant Pseudomonas aeruginosa infections in critical illness via anti-virulence monotherapyNature communicationsAugust 30, 2022Singh VK, Almpani M, Maura D, Kitao T, Ferrari L, Fontana S, Bergamini G, Calcaterra E, Pignaffo C, Negri M, et al.anti-rabbit (Catalog# ab150061; final concentration: 1:500; Abcam, Cambridge, MA, USA) and DAPI (Catalog# ab228549; Abcam, Cambridge, MA, USA) were added, and incubated 1 h at room temperature. The sections were then

(196) A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular EntrymBioAugust 30, 2022Liangqin Tong et al.room temperature. The nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam catalog no. ab228549). Images were examined by a Zeiss LSM 880 Meta confocal microscope. IC50 quantification. Vero cells

(197) Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosisExperimental & Molecular MedicineAugust 23, 2022Ran Gao et al.secondary antibodies. The nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, Cat#: ab228549, Britain). Images were photographed using a fluorescence microscope (Nikon, digital sight DS—Fi2, Japan).

(198) Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3iScienceAugust 19, 2022Ramirez MA, Ninoyu Y, Miller C, Andrade LR, Edassery S, Bomba-Warczak E, Ortega B, Manor U, Rutherford MA, Friedman RA, et al.were then rinsed three times in PBS for 15 minutes and stained with DAPI Staining Solution (Abcam, ab228549) for 5 minutes prior to mounting in ProLong Gold Antifade Mountant (Thermo Scientific, cat. P10144).

(199) Comparison of the different isoforms of vitamin e against amyloid beta-induced neurodegenerationTurkish Journal of BiologyAugust 16, 2022ALP YİĞİT ÖZDEMİR, ESİN ÇETİN, MEHMET ALİ ONUR(Cat number: A3890401) were purchased from Thermo Fisher Scientific, MA, USA. DAPI staining solution (ab228549), goat anti-mouse IgG H&L (Texas Red ®) (ab6787), and anti-MAP2 antibody [AP-20] (ab11268) were purchased

(200) SARS-CoV-2 Omicron Induces Enhanced Mucosal Interferon Response Compared to other Variants of Concern, Associated with Restricted Replication in Human Lung TissuesVirusesJuly 21, 2022Or Alfi et al.with PBST, and incubated with 4′,6-diamidino-2-phenylindole (DAPI, 10 uM, Abcam, Cambridge, UK, Cat# ab228549) as a nuclear stain. The following primary antibodies were used: α-E-Cadherin (Mouse monoclonal, 1:100,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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