DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(201) Small molecule SJ572946 activates BAK to initiate apoptosisiScienceOctober 21, 2022Sekar G, Singh G, Qin X, Guibao CD, Schwam B, Inde Z, Grace CR, Zhang W, Slavish PJ, Lin W, et al.HY-15141 AlexaFluor488-conjugated Annexin V,Abcam,Cat# ab219916 Propidium iodide,Abcam,Cat# ab14083 DAPI,Abcam,Cat# ab228549 AlexaFluor488-conjugated anti-cytochrome c antibody clone 6H2.B4,BioLegend,Cat# 612310 BMH crosslinker,Thermo Fisher Scientific

(202) Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic MiceeneuroOctober 20, 2022Pei He, Binjun He, Shu Li, Wen Chai, Wei Rao, Yu Zhu, Wenzhi Chen, Ping Zhang, Xiong Zhang, Haili Pan, et al.IgM/IgG H&L 1:100 (A10680, Life Tech Ltd.) conjugated to Alexa Fluor 488 for 2 h at RT and DAPI (Blue; ab228549, Abcam, Hong Kong Ltd.). The sections were then stained, extensively washed, mounted with antifade medium,

(203) Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic MiceeNeuroOctober 20, 2022Pei He, Binjun He, Shu Li, Wen Chai, Wei Rao, Yu Zhu, Wenzhi Chen, Ping Zhang, Xiong Zhang, Haili Pan, et al.IgM/IgG H&L 1:100 (A10680, Life Tech Ltd.) conjugated to Alexa Fluor 488 for 2 h at RT and DAPI (Blue; ab228549, Abcam, Hong Kong Ltd.). The sections were then stained, extensively washed, mounted with antifade medium,

(204) Electrical Stimulation of Human Adipose-Derived Mesenchymal Stem Cells on O2 Plasma-Treated ITO Glass Promotes Osteogenic DifferentiationInternational Journal of Molecular SciencesOctober 18, 2022Seungho Baek, Heekyung Park, Fatma Dilara Igci, Donghyun Lee(Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), ab150113 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647), ab150083) and DAPI staining solution (ab228549) used in immunohistochemistry experiments were purchased from Abcam (Cambridge, UK). 3.2. Experimental

(205) Polydatin Ameliorates High Fructose-Induced Podocyte Oxidative Stress via Suppressing HIF-1α/NOX4 PathwayPharmaceuticsOctober 16, 2022Ding H, Tang C, Wang W, Pan Y, Jiao R, Kong L.Gaithersburg, MD, USA) for 1 h at room temperature, and then stained with DAPI (4’,6-diamidino-2-phenylindole, ab228549, 1:2000, Abcam, Cambridge, MA, USA), at room temperature for 10 min. Finally, the sections were examined

(206) GPC3-Unc5 receptor complex structure and role in cell migrationCellOctober 13, 2022Akkermans O, Delloye-Bourgeois C, Peregrina C, Carrasquero-Ordaz M, Kokolaki M, Berbeira-Santana M, Chavent M, Reynaud F, Raj R, Agirre J, et al.,Fisher Scientific,Cat#12891660 Fetal Bovine Serum (FBS),GIBCO,Cat#10437028 Dapi Staining Solution,Abcam,Cat#ab228549 High Capacity Streptavidin Agarose Resin,Thermo Fisher Scientific,Cat#10733315 "Magnesium Sulfate,

(207) BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiencyNature communicationsOctober 5, 2022Wang F, Liu Y, Ni F, Jin J, Wu Y, Huang Y, Ye X, Shen X, Ying Y, Chen J, et al.oocytes were incubated for 1 h at room temperature with secondary antibodies (Thermo Fisher) and DAPI (ab228549, Abcam, U.K.). Negative controls included parallel oocytes that were treated without primary antibodies.

(208) SMPDL3b modulates radiation-induced DNA damage response in renal podocytesFASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 1, 2022Francis M, Ahmad A, Bodgi L, Azzam P, Youssef T, Abou Daher A, Eid AA, Fornoni A, Pollack A, Marples B, et al.BSA in TBS-T. After three wash with TBS-T, sections were stained with DAPI Staining Solution (cat # ab228549, Abcam, USA) for 30 min. Sections were washed three times with TBS with mild agitation. Finally, sections

(209) A Nesprin-4/kinesin-1 cargo model for nuclear positioning in cochlear outer hair cellsFrontiers in Cell and Developmental BiologySeptember 23, 2022Shahar Taiber, Oren Gozlan, Roie Cohen, Leonardo R. Andrade, Ellen F. Gregory, Daniel A. Starr, Yehu Moran, Rebecca Hipp, Matthew W. Kelley, Uri Manor, et al.myosin VIIa (Proteus Biosciences, 25-6790) 1:250, rabbit anti-FLAG (Abcam, ab205606) 1:100, DAPI (Abcam ab228549) 1:1,000, and goat anti-rabbit Alexa Fluor 488 (Cell Signaling 4412s) 1:250. Image analysis All data

(210) A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular EntrymBioAugust 30, 2022Liangqin Tong et al.room temperature. The nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam catalog no. ab228549). Images were examined by a Zeiss LSM 880 Meta confocal microscope. IC50 quantification. Vero cells

(211) Tackling recalcitrant Pseudomonas aeruginosa infections in critical illness via anti-virulence monotherapyNature communicationsAugust 30, 2022Singh VK, Almpani M, Maura D, Kitao T, Ferrari L, Fontana S, Bergamini G, Calcaterra E, Pignaffo C, Negri M, et al.anti-rabbit (Catalog# ab150061; final concentration: 1:500; Abcam, Cambridge, MA, USA) and DAPI (Catalog# ab228549; Abcam, Cambridge, MA, USA) were added, and incubated 1 h at room temperature. The sections were then

(212) Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosisExperimental & Molecular MedicineAugust 23, 2022Ran Gao et al.secondary antibodies. The nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, Cat#: ab228549, Britain). Images were photographed using a fluorescence microscope (Nikon, digital sight DS—Fi2, Japan).

(213) Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3iScienceAugust 19, 2022Ramirez MA, Ninoyu Y, Miller C, Andrade LR, Edassery S, Bomba-Warczak E, Ortega B, Manor U, Rutherford MA, Friedman RA, et al.were then rinsed three times in PBS for 15 minutes and stained with DAPI Staining Solution (Abcam, ab228549) for 5 minutes prior to mounting in ProLong Gold Antifade Mountant (Thermo Scientific, cat. P10144).

(214) Comparison of the different isoforms of vitamin e against amyloid beta-induced neurodegenerationTurkish Journal of BiologyAugust 16, 2022ALP YİĞİT ÖZDEMİR, ESİN ÇETİN, MEHMET ALİ ONUR(Cat number: A3890401) were purchased from Thermo Fisher Scientific, MA, USA. DAPI staining solution (ab228549), goat anti-mouse IgG H&L (Texas Red ®) (ab6787), and anti-MAP2 antibody [AP-20] (ab11268) were purchased

(215) SARS-CoV-2 Omicron Induces Enhanced Mucosal Interferon Response Compared to other Variants of Concern, Associated with Restricted Replication in Human Lung TissuesVirusesJuly 21, 2022Or Alfi et al.with PBST, and incubated with 4′,6-diamidino-2-phenylindole (DAPI, 10 uM, Abcam, Cambridge, UK, Cat# ab228549) as a nuclear stain. The following primary antibodies were used: α-E-Cadherin (Mouse monoclonal, 1:100,

(216) Targeting Adiponectin Receptor 1 Phosphorylation Against Ischemic Heart FailureCirculation ResearchJuly 8, 2022Di Zhu, Zhen Zhang, Jianli Zhao, Demin Liu, Lu Gan, Wayne Bond Lau, Dina Xie, Zhijun Meng, Peng Yao, Jumpei Tsukuda, et al.kit,Sigma-Aldrich,https://www.sigmaaldrich.com/US/en/product/sigma/duo94001 DAPI,Abcam,https://www.abcam.com/dapi-staining-solution-ab228549.html In-Fusion HD cloning kit,Takara,https://www.takarabio.com/search-results?term=in-Fusion+HD+cloning+kit&tab=product Superfect

(217) Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal RouteVirusesJune 26, 2022Minyue Qiu et al.(Servicebio, Wuhan, China) for 2 h. Finally, sections were stained with 4,6-diamidino-2-phenylindole (DAPI) (ab228549, Abcam, Cambridge, UK) for 4 min. Representative sections were scanned using a Pannoramic DESK digital

(218) Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanicsSTAR protocolsJune 17, 2022Deliorman M, Glia A, Qasaimeh MA.monoclonal CK5 antibodies (Alexa 647),Abcam,ab28106 "4′,6-diamidino-2-phenylindole (DAPI) dye",Abcam,ab228549 ,, Other,Other,Other ,, Pre-cleaned glass slides (75 mm × 25 mm × 1 mm),J. Melvin Freed,301M Sterile

(219) Involvement of NLRP3-inflammasome pathway in noise-induced hearing lossNeural Regeneration ResearchJune 6, 2022Wei-Wei Guo, Yuan-Yuan Yang, Long Sai, Yuan-Yuan Yang, Qing-Qing Jiang, Wei-Wei Liu, Qing-Qing Jianghour at room temperature, and for 10 minutes with a propidium iodide (Sigma, P4170) or DAPI (Abcam, ab228549) counterstain (5 µg/mL in PBS). Six cochleae were stained for caspase-1 and IL-1β in the noise exposure

(220) miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1Journal of Immunology ResearchJune 2, 2022Yongtao Yang, Xiaolan Sun, Minghua Li, Limei Li, Shanshan Wang, Yaomin Zhureflect the efficacy of chemotherapeutic drugs and was detected by immunofluorescence. Ki-67 antibody (ab228549, Abcam, USA) was used to label the active cells in each group cultured in 2.2. After incubating in the

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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