DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(121) Antibody–Drug Conjugate Sacituzumab Govitecan Enables a Sequential TOP1/PARP Inhibitor Therapy Strategy in Patients with Breast CancerClin Cancer ResMay 6, 2024Aditya Bardia, Sheng Sun, Nayana Thimmiah, James T. Coates, Bogang Wu, Rachel O. Abelman, Laura Spring, Beverly Moy, Phoebe Ryan, Mark N. Melkonyan, et al.for 1 hour. The cells were then washed twice with 2% BSA in PBS and counterstained with DAPI (Abcam, #ab228549). For flow cytometry detection of apoptotic cells, 1 × 106 cells were collected, and the staining was

(122) Acupuncture Ameliorates Depression-Like Behaviors Through Modulating the Neuroinflammation Mediated by TLR4 Signaling Pathway in Rats Exposed to Chronic Restraint StressMolecular NeurobiologyMay 1, 2024Huili Jiang, Xianqi Long, Yu Wang, Xuhui Zhang, Lu Chen, Xinjing Yang, Bingcong Zhao, Ye Zhang, Yemao Chai, Tuya Baowashed five times with PBS. And the nucleus was restained with DAPI staining solution (1:1000, Abcam, ab228549, USA) and then incubated at room temperature for 5 min. The slides were washed with PBS for 3 times,

(123) An integrated analysis revealing the angiogenic function of TP53I11 in tumor microenvironmentHeliyonApril 30, 2024Bai W, Ren JS, Li KR, Jiang Q.HUVECs were stained with rhodamine-conjugated phalloidin (Abcam, ab235138, 1:1000) and DAPI (Abcam, ab228549, 1:1000) to visualize the actin cytoskeleton and nuclei, respectively. 2.14 Western blot Proteins

(124) Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healingNature communicationsApril 23, 2024Han X, Saengow C, Ju L, Ren W, Ewoldt RH, Irudayaraj J.Secondary Antibody (1:50). ROS/Superoxide detection assay kit (ab139476), calcein AM (ab141420), DAPI (ab228549) were purchased from Abcam. Human mesenchymal stem cell marker antibody panel was purchased from R&D systems

(125) Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assemblyJournal of Cell BiologyApril 1, 2024S. John Calise, Audrey G. O’Neill, Anika L. Burrell, Miles S. Dickinson, Josephine Molfino, Charlie Clarke, Joel Quispe, David Sokolov, Rubén M. Buey, Justin M. Kollmanthen with secondary antibodies diluted in PBS for 1 h at RT in the dark, and finally with 5 µM DAPI (ab228549; Abcam) in PBS for 20 min at RT in the dark. The slide was mounted with ProLong Glass Antifade Mountant

(126) Mitochondrial fusion and altered beta-oxidation drive muscle wasting in a Drosophila cachexia modelEMBO ReportsMarch 1, 2024Callum Dark, Nashia Ali, Sofya Golenkina, Vaibhav Dhyani, Ronnie Blazev, Benjamin L Parker, Kate T Murphy, Gordon S Lynch, Tarosi Senapati, S Sean Millard, et al.antibodies conjugated to Alexa 488 and Alexa 555 (Molecular Probes) were used at 1:200. DAPI 405 (Abcam, #ab228549) was used at 1:10,000, Phalloidin 647 (Abcam, #ab176759) was used at 1:10,000, HCS LipidTOXTM Red Neutral

(127) Nature-inspired peptide of MtDef4 C-terminus tail enables protein delivery in mammalian cellsScientific ReportsFebruary 26, 2024Lucia Adriana Lifshits, Yoav Breuer, Marina Sova, Sumit Gupta, Dar Kadosh, Evgeny Weinberg, Zvi Hayouka, Daniel Z. Bar, Maayan Galthe PFA. All samples were stained with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI Staining Solution ab228549, Abcam). Cav-1 (ab2910, Abcam), β-tubulin (05-661, Merck). Images were taken directly from the 96-well

(128) MiR-155 promotes compensatory lung growth by inhibiting JARID2 activation of CD34+ endothelial progenitor cellsPLOS ONEFebruary 23, 2024Li Zhao, Jing Peng, Li Zhuang, Zhiling Yan, Fei Liao, Yifan Wang, Shihao Shao, Weiwei Wangincubated for 1 hour at 37°C, and washed 3 times with PBS and then 1 time with distilled water. DAPI (ab228549, Abcam, UK) was added to the stained specimen, 50% buffered glycerol was added to mount the specimen,

(129) Genomic and epigenomic basis of breast invasive lobular carcinomas lacking CDH1 genetic alterationsnpj Precision OncologyFebruary 12, 2024Higinio Dopeso, Andrea M. Gazzo, Fatemeh Derakhshan, David N. Brown, Pier Selenica, Sahar Jalali, Arnaud Da Cruz Paula, Antonio Marra, Edaise M. da Silva, Thais Basili, et al.labeled with Alexa-488 (E-Cadherin (#3199) Cell signaling, MA, USA; dilution 1:200). DAPI (Abcam # ab228549) was used as nuclear counterstaining. Fluorescent images were acquired using a TCS SP5 inverted confocal

(130) Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In VivoACS OmegaFebruary 6, 2024Zahra Khosrowpour, Seyed Mahmoud Hashemi, Samira Mohammadi-Yeganeh, Sara Simorgh, Behnaz Sadat Eftekhari, Peiman Brouki Milan, Subhas C. Kundu, Mazaher Gholipourmalekabadiovernight. The samples were then incubated with a secondary antibody (ab6785, Abcam) for 1 h at RT. DAPI (ab228549, Abcam) was used to counterstain the cells’ nuclei. The cells were viewed under a fluorescence microscope

(131) The Fuzzy planar cell polarity protein (FUZ), necessary for primary cilium formation, is essential for pituitary developmentJournal of AnatomyFebruary 1, 2024Emily J. Lodge, William B. Barrell, Karen J. Liu, Cynthia L. Andoniadousheep serum for 1 h and then washed again. Finally, all slides were incubated in DAPI (1:5000, Abcam ab228549) in PBS for 10 min, then washed in PBS and coverslips were mounted with Vectashield Antifade Mounting

(132) The Lnc‐ENST00000602558/IGF1 axis as a predictor of response to treatment with tripterygium glycosides in rheumatoid arthritis patientsImmunity, Inflammation and DiseaseJanuary 16, 2024Yang Gao, Xiaoyue Wang, Yanfeng Gao, Jian Bai, Yanpeng Zhao, Renyi Wang, Hanzhou Wang, Guangzhao Zhu, Xixi Wang, Xiaochen Han, et al.conjugated fluorescent dye (Abcam, ab150129, 1:1000) at 37° for 1 h. After counterstaining with DAPI (Abcam, ab228549, 1:5000), images were captured with a Nikon Eclipse TS100 microscope equipped with Micro‐Manager 1.4.22

(133) Biotin-cGMP and -cAMP are able to permeate through the gap junctions of some amacrine cells in the mouse retina despite their large sizeFront Ophthalmol (Lausanne)January 15, 2024Chunxu Yuan, Luca Gerhards, Ilia A. Solov’yov, Karin DedekRRID: AB_2535792). To visualize cell nuclei, incubation in 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, ab228549, Abcam) followed. After several washing steps, retinas were mounted on slides and coverslipped with

(134) Acute seizure activity in neonatal inflammation-sensitized hypoxia-ischemia in micePLOS ONEJanuary 11, 2024Angelina June, Weronika Matysik, Maria Marlicz, Emily Zucker, Pravin K. Wagley, Chia-Yi Kuan, Jennifer Burnsedon free-floating 40μm coronal sections [28]. 4’,6-diamidino-2-phenylindole (DAPI staining solution; ab228549; Abcam, Cambridge, United Kingdom) staining nuclear DNA was utilized to visualize anatomy and tissue

(135) CDK4/6 inhibitors display a class effect in inducing differentiation of neuroblastoma cellsWellcome open researchJanuary 1, 2024Ferguson KM, Abou Grealy FMY, Philpott A.blocking solution (1 hr, RT) and washed again. Nuclear counterstaining was performed using DAPI (Abcam, ab228549) (1:10000 in PBST, 15 min, RT). Imaging was performed on a Leica DMI 6000B Matrix microscope with Leica

(136) The effect of human PBMCs immobilization on their Аβ42 aggregates-dependent proinflammatory state on a cellular model of Alzheimer''s diseaseFrontiers in neuroscienceJanuary 1, 2024Kot K, Kot Y, Kurbanov R, Andriiash H, Tigunova O, Blume Y, Shulga S.cell morphology was assessed by confocal microscopy after staining nuclear DNA with DAPI dye (Abcam, ab228549, λEx – 405 nm, λEm = 461 nm). The hPBMCs studied included all mononuclear blood cells, such as lymphocytes,

(137) Cellular and molecular characterization of peripheral glia in the lung and other organsPloS oneJanuary 1, 2024Hall S, Liu S, Liang I, Schulz S, Ezran C, Tan M, Kuo CS.antibodies (Table 2). Sections were incubated with 4′, 6-diamidino-2-phenylindole (DAPI) (1:10,000, ab228549; Abcam, USA) for 1hr, fixed for 1h in 4% paraformaldehyde, washed with PBS, and placed in Cubic 1 solution

(138) Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancerJournal of food and drug analysisDecember 15, 2023Khoo SH, Wu PR, Yeh KT, Hsu SL, Wu CH.Scientific Hoechst 33342,62249,Thermo Fisher Propidium iodide (PI),P3566,Thermo Fisher Scientific DAPI,ab228549,Abcam HMGB1 recombinant protein,ab167718,Abcam Listed of chemicals used in this study

(139) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun Wang(#A-11008, Invitrogen) for 1 h. Tissues were mounted in PBT buffer and stained with DAPI Staining Solution (#ab228549, Abcam). A FLUOVIEW FV3000 confocal laser scanning microscope (Olympus, Shinjuku, Japan) was used to

(140) Single-cell profiling reveals transcriptomic signatures of vascular endothelial cells in non-healing diabetic foot ulcersFront Endocrinol (Lausanne)December 1, 2023Yangzhou Lu, Xiaogang Liu, Jingling Zhao, Fan Bie, Yiling Liu, Julin Xie, Peng Wang, Junyou Zhu, Yahui Xiong, Shitian Qin, et al.Biosciences,Servicebio,Servicebio,Abcam Catalog Number,AF6191,AF0931,DF6191,AF1009,AF5176,S0001,G1222,G1223,ab228549 Dilution,1:200,1:200,1:100,1:200,1:100,1:200,1:500,1:500,1:10000 Serum sPD-L1 changes before and

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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