T4 DNA Ligase from New England Biolabs

Product Specs
ItemT4 DNA Ligase
CompanyNew England Biolabs
Price $68.00 Inquire
Catalog NumberM0202S
Quantity20000 units
ApplicationsDNA Manipulation
Concentration400 u/µl
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One NEB unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of Lambda DNA in 30 minutes at 16°C in 20 µl of the above assay mixture and a 5´ DNA terminiconcentration of 0.12 µM (300 µg/ml).

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Arrayed dual-gRNA CRISPR Screening Platform for C9orf72 Repeat Expansion Excision in Patient iPSCsMolecular Therapy AdvancesJune 11, 2026Olubankole Aladesuyi Arogundade, Katie Jing Kay Kay Lam, Katherine A. Brown, Tanya Jain, Patrick O. Issagholian-Lewin, Cerianne Huang, Taylor Rae-Hudson, Kevin Briseno, Stacia K. Wyman, Netravathi Krishnappa, et al.buffer (4 M guanidine thiocyanate, 40 mM TRIS, 17.6 mM EDTA, pH 8.0, and TRIS 1 M pH 8). T4 Ligase (NEB M0202L) and HiFi HotStart Uracil+ Ready Mix (Kapa Biosystems KK2802) were used to repair gaps generated in

(2) Development of bicistronic plasmids and fusion proteins for clinical translation of tumor immune reprogrammingMolecular Therapy AdvancesJune 11, 2026Joanna Yang, Sabrina S. Chen, Ethan Idnani, Sydney R. Shannon, Kathryn Luly, Charina S. Fabilane, D. Scott Wilson, Jamie B. Spangler, Stephany Y. Tzeng, Jordan J. Greencatalog no. pUNO1-mcs) was digested with NheI and SalI. Ligation was performed using T4 DNA ligase (NEB, M0202S). Sequences of plasmids were verified via Sanger sequencing. Polymer synthesis PBAEs were synthesized

(3) De novo promoters emerge more readily from random DNA than from genomic DNASci AdvMay 29, 2026Timothy Fuqua, Andreas Wagnerand downstream of the adenylated genomic DNA, we used a T4 ligase master mix (NEB, USA, product no. M0202), with 3 μl of 10× T4 buffer, 1 μl of the upstream annealed adaptor (100 μM), 1 μl of the downstream

(4) Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expressionNucleic Acids ResMay 26, 2026Zhiyun Yang, Zongchao JiaZnCl2, 1 mM DTT, and 10 mM NaCl at 20°C for 30 min. Followed by adding 600 units of T4 DNA ligase (NEB, #M0202L) and incubating for 10 min at room temperature. After incubation, 30 mM EDTA, 1× NEB loading dye (NEB,

(5) Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stressSci AdvMay 22, 2026Michael C. Mazzola, Ting Zhao, Anna Kiem, Trine A. Kristiansen, Karin Gustafsson, Lai Ping Wong, Emily Scott-Solomon, Marissa D. Fahlberg, Christina Mayerhofer, Ernst Mayerhofer, et al.digestion on the aforementioned constructs, followed by blunting (NEB, #E1201) and T4 DNA ligation (NEB, M0202), per the manufacturer’s instructions. Lentivirus was prepared by transfecting low-passage HEK293T with

(6) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi ChenR0146S, Ipswich, MA, USA), followed by ligation using T4 DNA ligase (New England Biolabs Inc., Cat. No.: M0202S, Ipswich, MA, USA) to generate the recombinant plasmids pcDNA3.1-CTR1, pcDNA3.1-CDK4, and pcDNA3.1-ASH1L.

(7) Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene SilencingBio ProtocMay 20, 2026Angelica Severino, Concetta Lauro, Marzia Calvanese, Ermenegilda Parrilli, Maria Luisa Tutino(New England BioLabs, catalog number: N0447) 20. T4 DNA ligase (New England BioLabs, catalog number: M0202) 21. Genomic DNA of PhTAC125 (available upon request at this laboratory) 22. NotI-HF (New England BioLabs,

(8) Tollip antagonizes ESCRT-III-mediated plasma membrane repair and cell recoveryCell RegenMay 19, 2026Julia M. Ferrick, Xinan Meng, Emily Morgan, Anthony Kalvi, Zhen Li, Chaoqun Li, Chao-Yuan Chang, Suhong Xu, Yi-Nan Gongdigested products was purified (QIAGEN, Cat No. 28,706), and ligated using T4 DNA Ligase (NEB, Cat No. M0202S). Competent cells (NEB, Cat No. C3019H) were transformed with ligation products, and colonies were screened

(9) Cellular-state control using ribozyme-scaffolded miRNA-sensing and CRISPR-mediated actuationCell Reports MethodsMay 18, 2026Taek Kang, Leonidas BlerisMolecular cloning Restriction enzymes, Q5 High-Fidelity 2X Master Mix (NEB, catalog #M0492) and T4 DNA Ligase (NEB, catalog #M0202S) were purchased from New England Biolabs. All primers were synthesized by Sigma Aldrich. PCR and Gel

(10) A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signalingSci AdvMay 15, 2026Miroslav Micka, Jitender Kumar, Petra Paclíková, Zuzana Hayek, Kateřina Hanáková, Cherine Bechara, Hana Plešingerová, Ondrej Šedo, Sara Bologna, Elise Del Nero, et al.Antarctic phosphatase (M0289, NEB), and the DVL, CK1ε, or CK1δ constructs were ligated using T4 DNA Ligase (M0202, NEB). The pETM11 and pET-Z vectors contain a kanamycin resistance marker, and the pCDF-11 vector contains

(11) Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivoPLoS PathogMay 13, 2026Minetaro Aritakit (Takara Bio, #740609.250). T4 DNA ligase buffer (NEB, #B0202S) (3 μL) and T4 DNA ligase (NEB, #M0202L) (800 U, 2 μL) were added to the purified RT-PCR products (25 μL). The samples were incubated at room

(12) A multiplex, prime editing framework for identifying drug resistance variants at scaleCell GenomicsMay 13, 2026Florence M.C. Abadie, Chase C. Suiter, Nahum T. Smith, Riza M. Daza, Mary C. Rominger, Phoebe Parrish, Troy A. McDiarmid, Jean-Benoît Lalanne, Beth Martin, Diego Calderon, et al.BsaI-HFv2 (New England Biolabs, Cat. No. R3733S), 0.5 μL of T4 DNA ligase (New England Biolabs, Cat. No. M0202S) and 1 μL of 10X T4 DNA ligase reaction buffer (New England Biolabs, Cat. No. B0202S) in a final volume

(13) Functional chromatin signatures premark future lineage-specific enhancersCell GenomicsMay 13, 2026Julian Pulecio, Zakieh Tayyebi, Dingyu Liu, Wilfred Wong, Renhe Luo, Jeyaram R. Damodaran, Samuel J. Kaplan, Nan Hu, Hyein S. Cho, Jielin Yan, et al.ligation buffer (NEB, B0202), ATP (NEB, P0756S), 10% Triton X-100, 20mg/mL BSA and T4 DNA Ligase (NEB, M0202) ON at 16°C (gently rocking) followed by 30min at RT. Then, ligated samples were treated with proteinase

(14) Intracellular GRP78-Directed Delivery of Rapamycin by Biomolecular Condensates of Hydra-Elastin-like PolypeptidesBiomacromoleculesMay 11, 2026Sara Aly Attia, Sambid Adhikari, Amy S. Lee, John Andrew MacKayrespectively. The L-peptide oligo with sticky ends was then inserted using T4 DNA ligase (New England Biolabs #M0202) into the 5FV linear plasmid at a 3:1 molar ratio, and this insertion was incubated overnight at 4 °C

(15) In Vivo Massively Parallel Reporter Assay Reveals Sequence Determinants of mRNA Localization in AstrocytesbioRxivMay 4, 2026Sarah K Koester, Kristina Sakers, Gareth M Rurak, Stephen P Plassmeyer, K. McFarland McFarland White, S. Alves Ferreira Alves Ferreira Dias, Peter Baird, Dina J Kornbluth, Joseph DoughertyMini Kit (Macherey-Nagal, 740609.50). Constructs were ligated into plasmid with T4 DNA Ligase (NEB, M0202). All plasmids were verified by sequencing through Plasmidsaurus. Postnatal Astrocyte Labeling by Electroporation

(16) Archaeal family B DNA polymerase facilitates lagging strand DNA replication in the ThermococcalesNucleic Acids ResearchMay 4, 2026Geraldy Lie Stefanus Liman, Marina D Black, Vladimir Potapov, Brett W Burkhart, Katherine Levine, Brenda Baker, Jaylin L Mandley, Sarah C Davidson, Andrew F Gardner, Thomas J Santangelo, et al.Subsequently, 45 µl of 10× T4 DNA ligase buffer (NEB B0202), 8 µl of water, and 30 µl of T4 DNA ligase (NEB M0202) were added for a final volume of 450 µl and incubated at 16°C overnight. The reaction products were

(17) Spatially Resolved in vivo CRISPR Screen Sequencing via Perturb-DBiTbioRxivMay 3, 2026Alev Baysoy, Xiaolong Tian, Feifei Zhang, Paul Renauer, Zhiliang Bai, Hao Shi, Haikuo Li, Bo Tao, Mingyu Yang, Archibald Enninful, et al.,Thermo Fisher Scientific,R0192 NEBuffer 3.1,New England Biolabs,B6003S T4 DNA ligase,New England Biolabs,M0202L SDS,Sigma-Aldrich,71736-500ML "Proteinase K, recombinant, PCR grade",Thermo Fisher Scientific,EO0491 Dynabead

(18) Defining a Midgestational Window for In Utero Genome Editing of the Fetal Murine CortexbioRxivMay 1, 2026Cameron R. Jackson, Máté Borsos, Nathan Appling, Carrie R. Jackson, Gerard M. Coughlin, Viviana Gradinarufragments, annealed, and ligated into expression cassettes using T4 DNA Ligase (New England Biolabs, M0202). Self-complementary pAAVs were generated from pscAAV-CAG-GFP, a gift from Mark Kay (Addgene, 83279). pAAV2/1

(19) PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assemblySci AdvApril 29, 2026Richa B. Shah, Yuanyuan Li, Ashley Person, Léonie Frigon, Nina Ðukić, Ela Kini, Raymond Chen, John M. Pascal, Michael S. Cohen, Ivan Ahel, et al.lentiCRISPR v2 plasmid (50 ng) was ligated with 1 μM final concentration oligo duplex using T4 ligase (NEB, M0202) according to the manufacturer’s recommendations and incubated overnight at 4°C. XL-1 blue competent

(20) OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopyNucleic Acids ResApril 28, 2026Sara De Bragança, Clara Aicart-Ramos, Ángel Rivera-Calzada, Raquel Arribas-Bosacoma, Mark S Dillingham, Oscar Llorca, Fernando Moreno-Herrerowithout further purification, ligated with the fragment using T4 DNA ligase (New England Biolabs, #M0202S). Escherichia coli DH5α competent cells (Thermo Fisher Scientific, #18265–017) were transformed with

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(1)

4/18/2016DNA Ligation Kit Benjamin GerovacThis is a good kit for DNA ligation. Good for ligating sticky and blunt ends with overnight incubation. Ligated DNA successfully transforms into bacteria.

Production of a Subtracted cDNA Library
July 01, 2001
Ligation of Linkers or Adapters to Double- Stranded cDNA
January 01, 1991

T4 DNA Ligase

T4 DNA Ligase from New England Biolabs

Specifications/Features

New England Biolabs

Citations (2482)

Reviews

Protocols

Production of a Subtracted cDNA Library

Ligation of Linkers or Adapters to Double- Stranded cDNA

T4 DNA Ligase from New England Biolabs

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