Mini Trans-Blot Module from Bio-Rad

Product Specs
ItemMini Trans-Blot Module
CompanyBio-Rad
Price $689.00Get Pricing
Catalog Number1703935
Quantity1EA
ApplicationsWestern Blot, Electrophoretic transfer
FormatWet
CapacityUp to two mini-gels

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Porcine serum maltase-glucoamylase: structure, kinetics, and inhibitionJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2026Ken Watanabe, Takayoshi Tagami, Chihiro Biwa, Masato Kawasaki, Naruhiko Adachi, Toshio Moriya, Toshiya Senda, Masayuki Okuyamasubjected to SDS-PAGE (8% T/2.7% C acrylamide gel) and transferred to a Sequi-Blot PVDF membrane using a Mini Trans-Blot cell (Bio-Rad, USA). Standard Towbin buffer (20 mM Tris, 192 mM glycine, pH 8.3, 0.1% SDS, and 20% (v/v)

(2) EB1010, a putative Christensenellaceae-derived microbial peptide, exhibits anti-inflammatory activity and therapeutic potential in ulcerative colitisGut MicrobesDecember 31, 2026Maria L. Orsini Delgado, Camille Baudesson de Chanville, Hélène Pfister, Laureen Bardouillet, William Farin, Philippe Rinaudo, Jessica Quessel, Francesco Strozzi, Hélène Pereira, Marc Maresca, et al.(Invitrogen™, ThermoFisher Scientific Inc). Proteins were transferred onto nitrocellulose membrane through a Mini Trans-Blot Cell (BioRad). Following blocking with 5% milk at RT for 1.5 h, membranes were incubated overnight at

(3) The nuclear envelope protein TMEM209 is an integral component of the nuclear pore complex and interacts with Nup210Journal of Cell ScienceJune 15, 2026David Kohlhause, Christiane Spillner, Violeta Alcalde Zapata, Christof Lenz, Henning Urlaub, Tobias Kohl, Stephan E. Lehnart, Larry Gerace, Ralph H. KehlenbachTris-HCl pH 9.0 anode buffer, respectively. Proteins were transferred to a PVDF membrane (Millipore) in a Mini Trans-Blot® Cell (Bio-Rad) using transfer buffer (25 mM Tris, 0.193 M glycine, 0.02% SDS, 20% ethanol) for 90 min

(4) Ablation of ACSS2 drives alteration to cardiac and hepatic proteomic landscapes in a tissue- and sex-specific mannerJournal of ProteomicsMay 30, 2026Alexis M. Winters, Jessica Wohlfahrt, Tiara Wolf, Ankita Sarkar, Suraj J. Patel, Jennifer Guergues, Brant R. Burkhardt, Stanley M. Stevens100 V until resolved. Proteins were transferred to methanol activated PVDF membranes with use of the Mini Trans-Blot Cell (Bio-Rad, Hercules, CA) for wet transfer at 200 mA for 2 h. After confirmation of protein transfer

(5) Guinea pig δβγ-ENaC is locked in an open state and uncoupled from regulation by proteasesPflugers ArchApril 25, 2026Rene Yufenyuy Lawong, Etang Collins Etang, Fabian May, Philipp Vorrat, Oliver Rauh, Mike Althaustransferred onto polyvinylidine difluoride (PVDF) membranes using either the wet blotting method with the Mini Trans-Blot cell (Bio-Rad Laboratories) or the semi dry blotting method with the Trans-Blot Turbo Transfer System

(6) ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate developmentNucleic Acids ResApril 20, 2026Cecile Otten, Marin Kutnjak, Christine Supina-Pavic, Marija Pranjic, Ivan Anticevic, Vanna Medved, Marta Popovictransferred to Polyvinylidene difluoride (PVDF) membranes (Roche, 03010040001) by wet transfer using a Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5% low-fat milk in TBST buffer [20 mM Tris–HCl (pH 7.5),

(7) Quercetin Attenuates Oxidative Stress and Immune Inflammation via Modulating Heme and ROS Pathways in Rats Fed Protein-Oxidized Soybean MealAntioxidants (Basel)April 18, 2026Zhiyong Wang, Peng Wang, Yanmin Zhou, Leli Wang, Su Zhuang(Thermo Fisher Scientific, USA). Electrophoresis was carried out using the Mini-PROTEAN electrophoresis system and Mini Trans-Blot transfer system (Bio-Rad, Hercules, CA, USA). Protein samples were separated by SDS-PAGE, with 8–12%

(8) Two-Step Engineering of Food-Grade Aspergillus oryzae via Endogenous Signal Peptides and Vesicle Trafficking Proteins to Enhance Carrier-Free Protein SecretionJ Fungi (Basel)April 18, 2026Sarocha Panchanawaporn, Nakul Rattanaphan, Sukanya Jeennor, Jutamas Anantayanon, Weerapong Woraprayote, Laphaslada Pumpuang, Thipphiya Karirat, Nuttamon Prompakdee, Kobkul Laoteng, Chanikul Chutrakulapparatus (Bio-Rad Laboratories). Proteins were transferred onto 0.2-μm nitrocellulose membranes using a Mini Trans-Blot system (Bio-Rad Laboratories). Membranes were blocked with OneStep Blocker (Bio-Helix) and incubated

(9) Enzymatically Controlled Release of Proteins and Peptides (ENCOREP): A Promising Alternative Secretion ApproachiScienceApril 17, 2026Daniel Ivanusic, Gregor Zipf, Josef Maier, Hubert Bernauer, Norbert Bannert(Carl Roth) and 10 g/L SDS pellets (Carl Roth). Separated proteins were transferred to a 0.2 μm PVDF Mini trans-blot membrane (Bio-Rad, München, Germany) and then blocked for 1 h with 10% low fat milk powder (Carl Roth)

(10) Regorafenib-Induced Stress Response Alters the Bioenergetic Profile of Osteosarcoma Cells and Modulates Gene Expression Associated with Metabolic regulation-a Potential Mechanism of Osteosarcoma Treatment-Related AdaptationCancer Manag ResApril 16, 2026Klaudia Marcinkowska, Anna Raciborska, Bożena Obmińska-Mrukowicz, Agnieszka Śmieszektransferred onto a PVDF membrane using 1× Tris-glycine buffer (Bio-Rad, Hercules, CA, USA) via the Mini Trans-Blot® system (Bio-Rad, Hercules, CA, USA) at 100 V for 60 minutes. The membranes were blocked for 1 hour

(11) Exploration of the In Vitro and In Vivo Neuroprotective Effects of Several Polyphenolics on LPS-Induced NeuroinflammationBiochem Res IntApril 13, 2026Mona Elkhatieb, Rasha A. Radwan, Doaa Abou El-Ezz, Christine Adel Sedky, Ulrike Breitinger, Nabila Hamdi, Amira Emad Abdelaziz, Sarah Atef Fahim, Ahmed M. Hafezdetermined using western blotting using the Bio‐Rad gel electrophoresis system, Mini‐PROTEAN Tetra Cell by Mini Trans‐Blot, and Bio‐Rad ChemiDoc gel documentation imaging system according to literature [53]. Caspase‐3 and GAPDH

(12) Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategyeLifeApril 9, 2026Lori B Koch, Tiasha Ghosh, Christos Spanos, Adele L MarstonAmersham-GE Healthcare) in transfer buffer (25 mM Tris, 1.5% glycine, 0.02% SDS, 10% methanol) in a Bio-Rad Mini Trans-Blot system or a semi-dry Amersham TE70 transfer unit. Membranes were blocked in 5% milk in PBS with 0.01%

(13) Generation of Nano-Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular VesiclesJ Extracell BiolApril 7, 2026Alexis Dogliani, Laura Fradale, Céline Valeille, Christian Cambillau, Emmanuelle Jacquin-Joly, Alain Roussel, Josiane Kassis-Sahyounanalysis, the samples were electrotransferred onto a nitrocellulose membrane (Cytiva #10600006) using the Mini Trans‐Blot Cell system (Bio‐Rad) at 200 mA for 1 h. The membrane was blocked with 3% (w/v) BSA in TBS + 0.1% (v/v)

(14) Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transferMicrobiology SpectrumApril 7, 2026Lucas G. Castellani, María Delfina Cabrera, Abril Luchetti, Juliet F. Nilsson, Julieta Pérez-Giménez, Luis Alfredo Bañuelos-Vazquez, Alma Alva, Daniel Wibberg, Tobias Busche, Jörn Kalinowski, et al.reducing SDS-PAGE and transferred onto polyvinylidene difluoride membranes by Western blotting, using a Mini Trans‐Blot transfer Cell (BioRad). Proteins were detected on the membrane using GFP antibody (3H9) (Chromotek).

(15) Synaptotagmin isoforms differentially regulate glutamate and GABA release in the lateral habenulabioRxivApril 4, 2026Dustin N. White, J. Keenan Kushner, Kelly E. Winther, Dillon J. McGovern, Tamara Basta, Zoe R. Donaldson, Charles A. Hoeffer, David H. Root, Michael H. B. Stowell150V/50mA/gel for 90 min) followed by a transfer to nitrocellulose membranes using wet transfer system (Mini Trans Blot Cell) at low intensity conditions (30V, 90mA, 16h, 4°C) in 1xSDS running buffer and 20% methanol. Transfer

(16) Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr 9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma modelNeural regeneration researchApril 1, 2026Li F, Li Z, Li S, Zhou H, Guo Y, Wang Y, Lei B, Miao Y, Wang Z.and then transferred to a polyvinylidene fluoride (PVDF) membrane (R&D, Minneapolis, MN, USA) using a Mini Transblot electrophoretic transfer system (Bio-Rad). To block non-specific adsorption, the PVDF membranes were

(17) BTP2, a store-operated calcium channel inhibitor, attenuates morphine antinociceptive tolerance in ratsFront NeurosciApril 1, 2026Weibo Xiao, Feng Xiao, Yingying Zhang, Lei Zengin a 10% SDS-PAGE and transferred to nitrocellulose (NC) membranes (Millipore, USA) using a Bio-Rad Mini Trans-Blot apparatus. The membranes were blocked with 5% non-fat milk in Tris-buffered saline with Tween 20 (TBST)

(18) Granulin loss and TMEM106B risk converge on lysosomal C-terminal fragment pathology in frontotemporal dementiabioRxivMarch 31, 2026Yi Zeng, Jian Xiong, Anastasiia Lovchykova, Thao Phuong Nguyen, Abigail Song, Semma W. Gitler, Monther Abu-Remaileh, Aaron D. Gitlerdifluoride membranes (Bio-Rad, 162–0115) at 250 mA for 2 hours using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell, 170–3930). Membranes were blocked in EveryBlot Blocking Buffer (Bio-Rad, 12010020)

(19) Genome-wide DNA supercoiling arises from transcription and SMC activity and mediates transcriptional negative feedbackbioRxivMarch 28, 2026Linying Zhu, Qian Yao, Charan Vemuri, Chongyi Chen1610158) for electrophoresis, followed by transfer to a LF-PVDF membrane (Bio-Rad, 1620261) using the Mini Trans-Blot Cell system (Bio-Rad). LF-PVDF membranes were then blocked by 5% nonfat milk for 1 h and incubated overnight

(20) Activation of the protective arm of renin-angiotensin system enhances mitochondrial turnover improving respiration and decreasing integrated stress response in a human Complex III deficiency modelbioRxivMarch 27, 2026Lucía Fernández-del-Río, Andrea Eastes, David Rincón Fernández Rincón Fernández Pacheco, Nikole Scillitani, Jasmine Garza, Matthew Dugan, Matheus Pinto Pinto de Oliveira, Pradnya Kadam, Iman Gauhar, Karel Erion, et al.V. Immunoblotting Proteins were transferred to a methanol-activated PVDF membrane (Thermo Fisher Scientific, 88520) in a Mini Trans-Blot cell (Bio-Rad) at a constant voltage of 100 V for 75 min on ice. In blots involving cellular fractions,

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(1)

8/14/2014Great Transfer Apparatus Smita SI use this apparatus to transfer proteins from SDS-PAGE gels to PVDF membranes for immunoblotting.