Description
Our team offers plasmid DNA library construction and design services for both bacteria libraries and phage libraries. Depending on your research application, our team can help design parameters and produce the library that makes sense for your research. If you already know your plasmid DNA library requirements, you can reach out to our team to begin your production. If you are unsure about your requirements and design parameters, our team has assembled resources below and outlined our service capabilities. You can always contact our plasmid DNA team for additional guidance.
Bacteria Library Construction Services - Biological carrier: Bacteria (e.g., E. coli)
- Genetic material: Plasmids or vectors inside bacteria
- Display: No display on surface; gene inside bacteria
- Screening type: DNA-based cloning and expression
- Library Clone Production Maximum: 100,000
Bacteria Library Construction Sample Submission Requirements - The complete sequence of the vector, backbone type, plasmid map (if possible)
- Library parameters including strategy, target diversity, and coverage level. Our facility can produce up to 104 variants for a bacteria library.
- 3 µg of wildtype plasmid
Bacteria Library Construction Deliverable - 2-4 µg of research grade of the plasmid library
- Electronic project report with sequence alignments, original sequencing data (if requested), and sample selection sequence verifications
Phage Library Construction Services - Biological carrier: Bacteriophage (phage particles)
- Genetic material: DNA inserted into phage genome encoding displayed peptides
- Display: Peptides/proteins displayed on phage coat
- Screening type: Binding and interaction via displayed proteins
- Library Clone Production Maximum: 1,000,000
Phage Library Construction Sample Submission Requirements - The complete sequence of the vector, backbone type, plasmid map (if possible)
- Library parameters including strategy, target diversity, and coverage level. Our facility can produce up to 105 variants for a bacteria library.
- 3 µg of wildtype plasmid
Phage Library Construction Deliverable - 2-4 µg of research grade of the plasmid library
- Electronic project report with sequence alignments, original sequencing data (if requested), and sample selection sequence verifications
Your plasmid delivery package will include: - High purity plasmid of the requested volume
- Project report with quality control report
- Optional plasmid scale-up services available, including midiprep, maxiprep, and gigaprep