Recombinant RNasin Ribonuclease Inhibitor from Promega

Product Specs
ItemRecombinant RNasin Ribonuclease Inhibitor
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2515
Quantity10,000u
Concentration20 – 40 U/µL
SourceRecombinant E. Coli

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.DTT, M-MLV reverse transcriptase (Invitrogen, 28025-021) and RNAs in ribonuclease inhibitor (Promega, N2515) (10 min, 25 °C; 50 min, 37 °C; 15 min, 70 °C). qPCR reactions were performed with 25x diluted cDNA,

(2) The unconventional G-protein AtYchF1 interacts with the ribosomal protein AtRPS7 to modulate selective translation for balancing plant growth and stress response in ArabidopsisPlant CommunicationsJuly 13, 2026Sau-Shan Cheng, Jacky Chi-Ki Chi-Ki Ngo, Yee-Shan Ku, Zhixia Xiao, Ming-Yan Cheung, Hon-Ming Lamml−1 cycloheximide, 100 μg ml−1 chloramphenicol, 40 U ml−1 RNasin ribonuclease inhibitor [cat. no. N2515, Promega, Madison, WI, USA], and 1× Halt Protease Inhibitor Cocktail [cat. no. 78430, Thermo Fisher

(3) CRISPR-mediated precise large fragment insertion in zygotes enables rapid generation of humanized immunoglobulin heavy-chain miceImmunityJuly 9, 2026Usha Nair, Madhav Akauliya, John E. Warner, Shivang Parikh, Larance Ronsard, Maria Ssozi, Larissa Rays Rays Wahba, Abigail G. Esposito, Brendon Kelley, Caitlin McCarthy, et al.Biosystems,Cat#KK2602 HotStarTaq DNA Polymerase,Qiagen,Cat#203205 RNasin® Ribonuclease Inhibitors (Recombinant),Promega,Cat#N2515 "CountBright™ Absolute Counting Beads, for flow cytometry",Thermo Fisher Scientific,Cat#C36950 SIGMAFAST™

(4) BxbI-mediated insertion of a 77kb human RET sensitive haplotype into the mouse genome to generate a humanized model of Hirschsprung diseasebioRxivJuly 6, 2026Ryan D. Fine, Benjamin E. Low, Jarod Rollins, Jon M. Laurent, Michael V. Wiles, Aamir Zuberi, Jef D. Boeke, Aravinda ChakravartipRDF90–1 (two concentrations tested: 0.5 and 30ng/μl), and RNasin® Ribonuclease Inhibitor (Promega, cat# N2515; at 0.2U/μl). pRDF90–1 was shipped at 4°C on wet ice in BAC buffer supplemented with polyamines (10

(5) Mature adipocytes lack functional Aryl Hydrocarbon Receptor - a study investigating its role in diet-induced obesity in miceWellcome Open ResJune 24, 2026Julia Vlachaki Walker, Benjamin Wiggins, Alice Burke, Marian Dore, Alessandro Sardini, Chris Schieringbuffer,Heparin,Fisher Scientific,BP2524–100,1 mg/ml IP buffer,DTT,Sigma,D0632-10G,2 mM IP buffer,RNAsin,Promega,N2515,0.2 U/μl IP buffer,Protease inhibitor,Sigma,P8340-5ML,1× High Salt Wash Buffer,"Tris, pH 7.5",Life

(6) Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalizationCell Commun SignalMay 18, 2026Marlon Alexander Gancino Guevara, Arefeh Kardani, Annika Schomisch, Sari Rasheed, Vida Mashayekhi, Emely Saccon, Nurzhan Abdukarimov, Nikolay Krasimirov Kirilov, Sabryna Junker, Agnes-Valencia Weiss, et al.(200 U/µL, cat. no. 18064022, Thermo Fisher Scientific), 0.4 µL RNAse inhibitor (40 U/µL, cat. no. N2515, Promega), 5 × 10− 3 M dithiothreitol (DTT), 1 M Betaine, 6 × 10− 3 M MgCl2, and 1 × 10− 6 M TSO (B-AAGCAGTGGTATCAACGCAGAGTACAT997,

(7) mTOR-NAA10-C7orf50 axis senses nutritional status to coordinate ribosome biogenesis and autophagySci AdvMay 15, 2026Jingyu Sun, Mei Yang, Qian Li, Jiawei Liang, Haiping Feng, Lijie Gao, Yun Wang, Hongmei Liu, Caixia Guo, Tie-Shan TangEDTA-free protease inhibitor cocktail, CHX (100 μg/ml), 1 mM DTT, RNase inhibitor (40 U/ml; Promega, N2515), and 1% Triton X-100]. The lysates were centrifuged at 12,000g for 10 min at 4°C. Then, equal amounts

(8) An active RNA transport mechanism into plant vacuolesNucleic Acids ResMay 14, 2026Brice E Floyd, Ang-Yu Liu, Elakkiya A Venkatesan, Min May Wong, Stephanie C Morriss, Yosia Mugume, Zakayo Kazibwe, Victoria Ridout, Xinjuan Luo, Gustavo C MacIntosh, et al.Drosophila RNA outside the vacuole. Ten microliters of Recombinant RNasein© Ribonuclease inhibitor (N2515, Promega) was added to inhibit RNaseA activity and samples were flash frozen in liquid nitrogen until

(9) Whole-protein screening and multi-modal profiling of antigen-specific CD4+ T cells at single-cell resolutionNat CommunMay 12, 2026Rongyu Zhang, Jingqi Qi, Michaela McKasson, Jongchan Choi, Vanessa Gutierrez, Conor Brennan, Sunga Hong, William Chour, Rachel H. Ng, Jingyi Xie, et al.96-well plates containing 12 uL of 1x lysis buffer (Takara, 635013) with RNAse inhibitor (Promega, N2515). The plates were spun down immediately after sorting and flash frozen on dry ice. When ready for sequencing

(10) Comparative Transcriptomics Reveals an Extracellular Worm Argonaute as an Ancestral Regulator of LTR RetrotransposonsGenome Biol EvolMay 8, 2026Isaac Martinez-Ugalde, Kyriaki Neophytou, Yenetzi Villagrana-Pacheco, Adriana Orrego Durañona, Lewis Stevens, Xiaochen Du, Rowan Bancroft, Jessica L Hall, Amy B Pedersen, Mark Blaxter, et al.then lysed in 1 ml of cold lysis buffer as above supplemented with 200 U/ml RNase inhibitors (Promega, N2515). Unlysed material was removed by centrifugation (16,100 rcf, 10 min, 4 °C), and the supernatant lysate

(11) Sequestration of growth cone surface proteins by cytoplasmic Lrrtm2 induces de novo amygdala innervation by cerebral cortex associative neuronsbioRxivApril 12, 2026Dustin E. Tillman, Omer Durak, Priya Veeraraghavan, John E. Froberg, Garrett Wheeler, Bogdan Budnik, Jeffrey D. MacklisHEPES, (Thermo #15630106), Halt protease inhibitor (Thermo #78442), and RNasin RNase inhibitor (Promega #N2515), then homogenized at 900 RPM in a glass-Teflon tissue grinder. Post-nuclear homogenate (PNH) was generated

(12) Dietary restriction shapes intergenerational ribosome abundance and early growth of Caenorhabditis elegans offspringPLoS BiolApril 9, 2026Sigma Pradhan, Klement Stojanovski, Ferdinand Dellemann, Sacha Psalmon, Joel Tuomaala, Nicholas E. Stroustrup, Benjamin D. Towbin1% Triton X100, 0.5mM DTT, 100 μg/ml CHX, 100U/ml RNase inhibitor (RNasin RNase inhibitor, Promega, N2515), and 10 μl/ml protease inhibitor cocktail (Sigma Aldrich, P8340) at 5cps in a SPEX 6750 Freezer/Mill

(13) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyApril 2, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.DTT, M-MLV reverse transcriptase (Invitrogen, 28025-021) and RNAs in ribonuclease inhibitor (Promega, N2515) (10 min, 25 °C; 50 min, 37 °C; 15 min, 70 °C). qPCR reactions were performed with 25x diluted cDNA,

(14) Optineurin is a gatekeeper of mitochondrial health and proteostasis in Alzheimer’s disease vulnerable neuronsbioRxivMarch 18, 2026Christina Tsagkogianni, Matteo Trivisonno, Johanna Sophie Willner, Carlos Garcia-Molinero, Yingyu Tang, Beatrice Mattina, Maria Latorre-Leal, Wei Wang, Jean-Pierre Roussarie, Patricia Rodriguez-Rodriguezsupplemented with protease inhibitors (#A32965, ThermoFisher) and RNAse inhibitors (40U/ml RNAsin, #N2515, Promega and 20U/ml Superasin, #AM2696, ThermoFisher). After centrifugation, the supernatant was incubated

(15) Local antibody feedback enforces a checkpoint on affinity maturation in the germinal center and promotes epitope spreadingImmunityMarch 10, 2026Yu Yan (颜雨), Xuesong Wang (王雪松), Zhenfei Xie, Daniel L. V. Bader, Ryan H. Lim, Krystal M. Ma, Christopher A. Cottrell, Jon M. Steichen, Liling Xu, Paula M. Villavicencio, et al.Cocktail",MilliporeSigma,Cat#: 12352204 RNasin® Ribonuclease Inhibitors (Recombinant),Promega,Cat#: N2515 "CountBright™ Absolute Counting Beads, for flow cytometry",Thermo Fisher Scientific,Cat#: C36950 SIGMAFAST™

(16) Dynamic subtype- and context-specific subcellular RNA regulation in growth cones of developing neurons of the cerebral cortexNature NeuroscienceMarch 1, 2026Priya Veeraraghavan, Anne K. Engmann, John J. Hatch, Yasuhiro Itoh, Duane Nguyen, Thomas Addison, Jeffrey D. Mackliswith 4mM HEPES, HALT protease and phosphatase inhibitors (#78442, Thermo), and 1U/mL RNAse inhibitor (#N2515, Promega). CPN GCs at P1 were collected from the corpus callosum in the ipsilateral cortical hemisphere,

(17) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.supplemented with 350 mM 2-mercaptoethanol (bME, Sigma Adlrich, M3148) and 2.5 µL RNAsin (Promega, N2515) in a RNAse-free 0.5 mL Eppendorf tube, immediately frozen at dry ice and subsequently subjected to

(18) Mechanistic insights into RNA chaperoning by Ro60 and La autoantigensCellFebruary 19, 2026Hyeyeon Nam, Justin C. Deme, Soyeong Sim, Marco Boccitto, Susan M. Lea, Sandra L. Wolinpre-5S rRNA; Table S3), 1X RNA ligase buffer (New England Biolabs), 1 U/μl RNase inhibitor (Promega N2515), and 20 U/μl T4 DNA ligase (New England Biolabs M0202S) in a volume of 10 μl, and was incubated 1 h

(19) The Intracellular C5a–mtC5aR1 Axis Promotes Necroptosis in Dry Eye Through DRP1-Mediated Mitochondrial DysfunctionInvestigative Ophthalmology & Visual ScienceFebruary 18, 2026Limei Wang, Haijing Yan, Yetao Shen, Songlin Xin, Chenyu Dong, Jiuxiao Li, Yueping Ren, Wei Chen, Qinxiang ZhengM-MLV reverse transcriptase (M1701; Promega), and Recombinant RNasin Ribonuclease Inhibitor (37°C, 1 hour; 95°C, 5 minutes) (N2515; Promega). Quantitative RT-PCR was conducted using ChamQ Universal SYBR qPCR Master Mix (Q711-03; Vazyme,

(20) Maternal SETDB1 enables development beyond cleavage stages by extinguishing the MERVL-driven 2-cell totipotency transcriptional program in the mouse embryoeLifeFebruary 16, 2026Tie-Bo Zeng, Zhen Fu, Mary F Majewski, Ji Liao, Marie Adams, Piroska E Szabókit,RNA-Bee,Amsbio,Cat. No. CS-105B, "Peptide, recombinant protein",RNasin ribonuclease inhibitor,Promega,Cat. No. N2515, Commercial assay or kit,DNA-free Kit,Ambion,Cat. No. AM1906, Commercial assay or kit,Agilent High

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