RNasin Ribonuclease Inhibitor from Promega

Product Specs
ItemRNasin Ribonuclease Inhibitor
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2115
Quantity10,000u
Concentration20 - 40 U/µL
SourceHuman Placenta

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Activity-dependent protein synthesis in neurons requires microglial-metabolic couplingCell MetabolismAugust 4, 2026Drew Adler, Alejandro Martín-Ávila, Evan Cheng, Mauricio M. Oliveira, Muxian Zhang, Harrison T. Evans, Deliang Yuan, Richard Sam, Nicole D. Zhang, Maria Clara Selles, et al.mM sucrose (Ambion), 10 mg ml–1 BSA (Ambion), 5 mM MgCl2 (Ambion), 0.12 U μl–1 RNasin Plus (Promega, N2115), and 1× Complete Protease Inhibitor Cocktail (Roche, 11697498001)) using a pre-chilled tissue grinder.

(2) Integrated Single-Nucleus Multi-Omics Atlases Reveal Lineage Plasticity and Regulatory Networks of Luminal Epithelial Cells During Mammary Gland Lactation and InvolutionAdv Sci (Weinh)July 27, 2026Xiaoru Yan, Xiaoyu Mi, Guanghui Tan, Xinmei Li, Zhenliang Zhu, Zhenyu Wei, Huimei Fan, Yamei Wu, Tao Shi, Lingzhao Fang, et al.10 mg/mL bovine serum albumin (BSA, Ambion), 5 mm MgCl2 (Ambion), 0.12 U/µL RNasin Plus (Promega, #N2115) and 1 × Complete Protease Inhibitor Cocktail (Roche, #11697498001) using a homogenizer. To ensure uniformity,

(3) Germline sequence variation within the ribosomal DNA is associated with human complex traitsCell GenomicsJune 10, 2026Francisco Rodriguez-Algarra, Elliott Whittaker, Maia Cooper, Sergey Koren, Maria R. Conte, Adam M. Phillippy, Faraz K. Mardakheh, David M. Evans, Vardhman K. Rakyansupplemented with 1mM dithiothreitol (Sigma-Aldrich Cat. No. D0632), 40 U/mL RNase inhibitor (Promega Cat. No. N2115) and 1x Pierce Protease Inhibitor Mini tablet (Thermo Fisher Scientific Cat. No. A32953) just before

(4) Cross-species comparison of amniote single-cell transcriptomes reveals evolutionary conservation and divergence in the chicken immune systemNature CommunicationsMay 5, 2026Fei Wang, Jilong Ren, Yaqi Zhou, Wuqiang Huo, Huichao Liu, Mumu Qin, Yi Jing, Zhongtao Yin, Tao Shi, Huiquan Shan, et al.sucrose, 10 mg/mL bovine serum albumin, 5 mM MgCl2, 0.12 U/μL RNasin Ribonuclease Inhibitor (Promega, #N2115), and 1× cOmplete Protease Inhibitor Cocktail (Roche, #11697498001). After two additional rounds of

(5) Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cellsCell ResearchApril 6, 2026Huihui Liu, Shangfeng Liao, Xiaowei Li, Li Song, Mu-Ming Poo, Jing Zhao, Weijun Zhou, Ruijie Cai, Meijian Wang, Xiaotong Ma, et al.(Thermo Fisher Scientific, #87786), 0.1 mM DTT (Sigma, #646563), and 0.12 U/µL RNasin Plus (Promega, #N2115). After incubation on ice for 5 min, the mixture was homogenized with 25 strokes of the loose Dounce

(6) Gestational choline supplementation regulates hippocampal granule neuron development and emotion-like behaviorCommunications BiologyApril 2, 2026Xiaohui Shi, Yuanyuan Li, Mengjiao Wang, Xinlan Zong, Min Liang, Quan Li, Weiwei Xiao, Zhongsheng Sun, Yan Wangcontaining 250 mM sucrose (Sigma-Aldrich, S8501), 10 mg mL–1 BSA, 5 mM MgCl2, 0.12 U μL–1 RNasin (Promega, N2115) and 1× Complete Protease Inhibitor Cocktail (Roche, 11697498001). Frozen tissues were kept in an ice

(7) Integrating single-nucleus RNA sequencing and gene perturbation reveals regulatory networks of intramuscular fat deposition in bovine skeletal muscleAnimal BioscienceMarch 11, 2026Fengying Ma, Mingjuan Gu, Lin Zhu, Risu Na, Caixia Shi, Guiting Han, Chencheng Chang, Le Zhou, Yanchun Bao, Shuai Li, et al.ice-cold homogenization buffer (250 mM sucrose, 10 mg/mL BSA, 5 mM MgCl2, 0.12 U/μL RNasin Plus [Promega, N2115], and 1× complete Protease Inhibitor Cocktail [Roche, 11697498001]) using 25–50 gentle strokes with

(8) Single‐Cell Transcriptomic Atlases of Camels and Cattle Unravel Molecular Evolution of Digestive and Metabolic SystemsAdvanced ScienceFebruary 3, 2026Tao Shi, Huiquan Shan, Haoping Wang, Xi Guo, Houcheng Li, Bo Han, Senlin Zhu, Fei Wang, Guanghui Tan, Zhannur Niyazbekova, et al.(Ambion), 10 mg/mL bovine serum albumin (Ambion), 5 mM MgCl2 (Ambion), 0.12 U/µL RNasin Plus (Promega, #N2115), and 1 × Complete Protease Inhibitor Cocktail (Roche, #11697498001). The tissue underwent two additional

(9) Microglia coordinate activity-dependent protein synthesis in neurons through metabolic couplingbioRxivJanuary 16, 2026Drew Adler, Alejandro Martín-Ávila, Evan Cheng, Mauricio M. Oliveira, Muxian Zhang, Harrison T. Evans, Deliang Yuan, Richard Sam, Nicole D. Zhang, Maria Clara Selles, et al.mM sucrose (Ambion), 10 mg ml–1 BSA (Ambion), 5 mM MgCl2 (Ambion), 0.12 U μl–1 RNasin Plus (Promega, N2115), 0.12 U μl–1 RNasein (Promega, N2115) and 1× Complete Protease Inhibitor Cocktail (Roche, 11697498001))

(10) A role for the cholinergic neuron circadian clock in RNA metabolism and mediating neurodegenerationLife Sci AllianceDecember 15, 2025Sharon B Tam, Nathan J Waldeck, Matthew Wright, Jelena Mojsilovic-Petrovic, Erin M Baker, Evangelos Kiskinis, Joseph Bass, Robert G Kalb12 mM MgCl2, 1% Tergitol (NP-40), 1 mM dithiothreitol (DTT), 200 U/ml RNasin Ribonuclease Inhibitor (N2115; Promega), 1 mg/ml heparin, 100 μg/ml cycloheximide, and Protease Inhibitor Cocktail (P8340; Sigma-Aldrich)

(11) Analyzing the Translatome of Lymphatic and Venous Endothelial Cells In Vivo via Translating Ribosome Affinity Purification (TRAP)Bio ProtocDecember 5, 2025Olamide Olayinka, Leia Zarinebaf, Hyun Min Jung23200) 6. 1 mM DTT (Sigma, catalog number: 646563) 7. 200 units/mL RNAsin (Promega, catalog number: N2115) 8. 1 mg/mL heparin (Sigma, catalog number: H3393-10KU) 9. 10% NP-40 (Roche, catalog number: 11-332-473-001) 10.

(12) Analyzing the Translatome of Lymphatic and Venous Endothelial Cells In Vivo via Translating Ribosome Affinity Purification (TRAP)BIO-PROTOCOLDecember 5, 2025Olamide Olayinka, Leia Zarinebaf, Hyun Min Jung23200) 6. 1 mM DTT (Sigma, catalog number: 646563) 7. 200 units/mL RNAsin (Promega, catalog number: N2115) 8. 1 mg/mL heparin (Sigma, catalog number: H3393-10KU) 9. 10% NP-40 (Roche, catalog number: 11-332-473-001) 10.

(13) A noncanonical cytoplasmic role for BUB1 restrains DNA damage-induced dsRNA accumulation and viral mimicry within stress granulesScience ImmunologyAugust 8, 2025Mengjie Hu, Dong Pan, Meng Jiao, Xuhui Bao, Xinjian Liu, Fang Li, Chuan-Yuan Liethylenediaminetetraacetic acid, containing protease inhibitors) with 100 U/ml RNase inhibitor (Promega N2115). After being kept on ice for 30 min, the cell lysate was centrifuged at 13,000 rpm for 15 min, and

(14) In vivo profiling of the endothelium using 'AngioTag' zebrafishAngiogenesisJuly 4, 2025Mayumi F. Miller, Leah J. Greenspan, Derek E. Gildea, Kathryn Monzo, Gennady Margolin, Van N. Pham, Keith K. Ameyaw, Lisa Price, Natalie Aloi, Amber N. Stratman, et al.(Sigma, Cat. #646563), 1 × Protease inhibitors (Sigma, Cat. #P8340), 200 units/mL RNAsin (Promega, Cat. #N2115), 100ug/mL Cycloheximide (Sigma Cat. #7698), 1mg/mL Heparin (Sigma, Cat. #H3393-10KU). Lysates were

(15) RiboTag-based RNA profiling uncovers oligodendroglial lineage-specific inflammation in autoimmune encephalomyelitis: implications for pathogenesisJ NeuroinflammationMay 21, 2025Yuhang Wang, Sudeep Ghimire, Ashutosh Mangalam, Zizhen Kang100 µg/ml cycloheximide, a protease inhibitor cocktail, 1 mg/ml heparin, RNase inhibitors (Promega, #N2115), and 1 mM dithiothreitol (DTT).The resulting homogenates underwent centrifugation, and the supernatant

(16) Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growthFront Cell Infect MicrobiolMay 2, 2025Xiaoqian Liu, Ningjian Cai, Youchao Dai, Xinchun Chen, Xiaobin Zeng1% TritonX-100, 1 mM DTT) supplemented with protease inhibitors and an RNase inhibitor (Promega, cat#N2115). The lysate was centrifuged, and the supernatant was collected. Protein concentrations in the lysate

(17) Herpes simplex virus 1 encodes a STING antagonist that can be therapeutically targetedCell Reports MedicineApril 15, 2025An Wang, Qianqian Peng, Huidi Fan, Wenting Ji, Jing Lou, Xi Zhou, Yujie RenEN0521 Protease inhibitor cocktail,MCE,Cat# HY-K0010 ECL,4A Biotech,Cat# BN01036 RNase inhibitor,Promega,Cat# N2115 Protein A/G Magnetic Beads,MCE,Cat# HY-K0202 Streptavidin Magnetic beads,MCE,Cat# 563175 qPCR SYBR

(18) Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cellsFront OncolApril 14, 2025Yue He, Jian-Qing Xu, Jing-Jing Zhang, Zhen-You Liu, Chen Ji, Yang Liu, Yun-Fan Wang, Ming Wang, Yu-Mei Wu, Yan WangPu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-Loop™ miRNA qPCR Primer Set (Guangzhou Ruibo Company),

(19) Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered BreathingInt J Mol SciApril 11, 2025Bettina Seydel, Philipp Hegner, Anna-Maria Lauerer, Sönke Schildt, Fatma Bayram, Maria Tafelmeier, Dominik Wermers, Leopold Rupprecht, Christof Schmid, Stefan Wagner, et al.PCR nucleotide mix (Promega, catalog number C1145), RNasin® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog number M170B), and reverse transcriptase 5x reaction buffer

(20) Post-mortem interval leads to loss of disease-specific signatures in brain tissueeNeuroFebruary 27, 2025Kimberly C. Olney, Katelin A. Gibson, Mika P. Cadiz, Negin Rahimzadeh, Vivek Swarup, John D. Fryer(Miltenyi Biotec, #130-128-024), followed by Miltenyi debris removal. Promega RNase Inhibitor (Promega, #PR-N2115) was included in all buffers and wash steps at a final concentration of 0.2 U/µl to prevent RNA degradation.

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