RNasin Ribonuclease Inhibitor from Promega

Product Specs
ItemRNasin Ribonuclease Inhibitor
CompanyPromega
Price Get Pricing Get Quote for this Product
Catalog NumberN2111
Quantity2,500u
Concentration20 - 40 U/µL
SourceHuman Placenta

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cellsiScienceMay 15, 2026Yong-Dae Gwon, Sandra Haider, Martin Knöfler, Matthew Bradley, Johan Henriksson, Magnus Evander12605010 Deoxyribonuclease I (Dnase I) from bovine pancreas,Merch,Cat# DN25-100MG RNase inhibitor,Promega,Cat# N2111 Recombinant IFN-λ protein,R&D Systems,Cat# 1598-IL "UltraPure™ 0.5M EDTA, pH 8.0",Thermo Scientific,Cat#

(2) Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionCell RepApril 7, 2026Manuel Garcia-Moreno, Azman Embarc-Buh, Robin Truman, Marko Noerenberg, Louisa Iselin, Honglin Chen, Caroline E. Lenz, Jeffrey Y. Lee, Kate Dicker, Snehith Dyavari Shetty, et al.alkaline phosphatase (Thermo Fisher Scientific, #EF0654), 0.5 U TurboDNase and 20 U RNasin (Promega, #N2111). Beads were washed once with 500 μL cold PNK wash buffer, twice with the same volume of cold high-salt

(3) ENDOU-1-induced cytoplasmic HnRNPA3 recognizes m6A methylation on the upstream reading frame of human CHOP transcripts to achieve maximal CHOP translationCellular and Molecular Life SciencesMarch 28, 2026Hung-Chieh Lee, Yi-Hsin Huang, Chi-Cheng Hsieh, Yi-Nan Ke, Huai-Jen Tsai500 ng of protein extracts were incubated with 2 µg of biotinylated probes and 100U RNasin (Promega, N2111) at 4℃ for 1 h with gentle rocking. Then, 20 µL of Streptavidin beads (ThermoFisher, 20359) were added

(4) Regulation of oncogenic C-terminal truncated p53β protein isoform expression by SRSF3–UPF1 splicing and surveillance axisCell & BioscienceMarch 23, 2026Jiwon Jeong, Dawon Hong, Tae Young Park, Jung Hur, Taeyoung Koo, Sunjoo Jeong20 mM Tris–HCl at pH 7.9, 75 mM NaCl, 0.5 mM EDTA, 0.85 mM DTT, PPI, and 100 U of RNasin (Promega, #N2111). Next, 900 μL of buffer 2A—20 mM HEPES at pH 7.6, 300 mM NaCl, 0.2 mM EDTA, 1 mM DTT, 7.5 mM MgCl2,

(5) Protocol to uncover the protein interactome of small non-coding vault RNAs through RNA antisense purification coupled to mass spectrometrySTAR ProtocMarch 20, 2026Sander B. van der Kooij, Jorn E. Stok, Annemarthe G. van der Veen,Thermo Fisher Scientific,J60767.AD cOmplete protease inhibitor,Roche,11836153001 RNasin (RNase inhibitor),Promega,N2111 TURBO DNase,Invitrogen,AM2238 TURBO DNase buffer,Invitrogen,AM2238 RLT buffer,Qiagen,79216 UltraPure™

(6) SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasisbioRxivFebruary 3, 2026Shanshan Liu, Mark Gad, Caifan Li, Kevin Cho, Yuyang Liu, Khando Wangdu, Viktor Belay, Alon Millet, Hiroyuki Kojima, Henry Sanford, et al.RNA extraction For RNA extraction, ER-IP was performed in the presence of RNAese inhibitor (Promega, N2111, 1:400). For whole cell RNA extraction, cell pellets collected before ER purification were lysed with

(7) A non-canonical role for HIF-1α: redirecting DGCR8 to the RNA exosome for snoRNA degradation and translational modulationNucleic Acids ResFebruary 2, 2026Jie-Ning Li, Ming-Yang Wang, Chiao Lo, Laising Yen, Chien-Hung Yu, Yu-Jhen Lyu, Pai-Sheng Chenheated to 65°C for 5 min. Then, 4 µl 5X buffer, 0.6 µl reverse transcriptase, 0.4 µl RNasin® (Promega, N2111), and nuclease–free water were added to 20 µl total. Reactions were incubated at 25°C for 5 min, 37°C

(8) BCLAF1 links RNA splicing to ATF4-dependent metabolic adaptation in acute myeloid leukemiabioRxivJanuary 25, 2026Laura López-Hernández, Stephanie J. Crowley, Sara Cea-Sánchez, Ricardo de Arellano, Neetij Krishnan, Patrick Toolan-Kerr, Lynn White, Domenico Ignoti, Emily Soto-Hidalgo, Stefano Gustincich, et al.(Sigma-Aldrich, 11697498001), PMSF (Millipore, 52332) and 5 μL RNasin® Ribonuclease Inhibitor (Promega, N2111) every 1 mL of buffer). The IP was set using 300 μL NT2 buffer (50 mM Tris-HCl pH 7.5, 150 nM NaCl,

(9) Chronic stress antagonizes formation of stress granulesiScienceJanuary 16, 2026Yuichiro Adachi, Allison M. Williams, Masashi Masuda, Yutaka Taketani, Paul J. Anderson, Pavel IvanovExpress,HY-18982 Emetine,Sigma-Aldrich,E2375 Cycloheximide,Sigma-Aldrich,C7698 PureLink RNase A,Invitrogen,12091021 RNasin Ribonuclease Inhibitor,Promega,N2111 HEPES,Gibco,15630080 Superscript IV first-strand synthesis kit for RT-qPCR,Invitrogen,18091050 iQ™ SYBR® Green Supermix

(10) Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domainsMolecular CellJanuary 8, 2026Lin Guo, Jacob R. Mann, Jocelyn C. Mauna, Katie E. Copley, Hejia Wang, Jack D. Rubien, Cristian A. Bergmann, Jenny L. Carey, Jessica Merjane, Marilyn Ngo, et al.salt,Sigma-Aldrich,H3149-10KU Animal-Free Recombinant Human EGF,PeproTech,AF-100-15 RNasin® Ribonuclease Inhibitor,Promega,N2111 Recombinant Human FGF-basic (154 a.a.),PeproTech,100-18B GST-FUS,This paper,N/A GST-FUSC428A:C433A:C444A:C447A

(11) Mechanical forces regulate the composition and fate of stalled nascent chainsMolecular CellJanuary 8, 2026Danish Khan, Ananya A. Vinayak, Cole S. Sitron, Onn Brandman,Thermo Fisher Scientific,AC403160050 Coomassie Brilliant Blue,Bio-Rad,1610406 RNAsein® Ribonuclease Inhibitor,Promega,N2111 Critical commercial assays,, 4x NuPage LDS Sample Buffer,Thermo Fisher Scientific,NP0007 NEBuilder® HiFi DNA Assembly Master Mix

(12) A prelimbic molecular clock of protein synthesis for memory persistencebioRxivJanuary 5, 2026J. Iqbal, S. Kim, S. Lawal, A. Shah, L. Punepalle, H. Sanghvi, A. Gallagher, A. Wilson, B. Xu, P ShresthaKCl, 10 mM MgCl2, 1% NP-40, 0.5 mM DTT, 100 mg/ml cycloheximide, and 10 μl/ml RNasin (Promega Catalog# N2111) and 10 μl/ml SUPERase-In (Fisher Scientific Catalog # AM2696) RNase inhibitors on ice. The samples

(13) Double stranded RNA sensing is silenced during early embryonic developmentNat CommunDecember 11, 2025Jeroen Witteveldt, Zicong Liu, Ana Ariza-Cosano, Christian Ramirez, Jessica L. Walters, Pilar G. Marchante, Lars Maas, Elias T. Friman, Alasdair Ivens, Toma Tebaldi, et al.(S8820, Sigma-Aldrich). For each 1 ml of lysate, 10 μl DNAse (M6101, Promega) and 2 μl RNAse inhibitor (N2111, Promega) was added and incubated on ice for 30 min after which the lysate was spun at 13krpm, 4 °C

(14) Extended In Vitro Maturation Enhances Oocyte Developmental Competence but Alters Gene Expression in Bovine Embryos Derived From Oocytes With Slow‐Predicted Nuclear Maturation SpeedMolecular Reproduction and DevelopmentNovember 18, 2025Thomas Chia‐Tang Ho, Takashi Tanida, Takashi Fujii, Keisuke KoyamaIgepal [ICN Biomedical], 5 mM Dithiothreitol [P1171, Invitrogen], and 1 U/μL Ribonuclease Inhibitor [N2111, Promega]). The lysed samples containing blastocyst RNA were snap‐frozen at least 3 times in liquid

(15) Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditionsJ Tissue EngOctober 27, 2025Isaura Borges-Silva, Marluce da Cunha Mantovani, Minh Danh Anh Luu, Alan Gorter, Theo Borghuis, Naschla Gasaly, Mari Cleide Sogayar, Paul deVos, Marina Trombetta-LimaTranscriptase set (M-MLV RT; Promega, cat#M1701) and RNasin® Ribonuclease Inhibitor (Promega, Netherlands cat#N2111) were added, and samples were run at 25°C for 10 min, 37°C for 50 min and 70°C for 15 min. Gene expressions

(16) Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditionsJournal of Tissue EngineeringOctober 27, 2025Isaura Borges-Silva, Marluce da Cunha Mantovani, Minh Danh Anh Luu, Alan Gorter, Theo Borghuis, Naschla Gasaly, Mari Cleide Sogayar, Paul deVos, Marina Trombetta-LimaTranscriptase set (M-MLV RT; Promega, cat#M1701) and RNasin® Ribonuclease Inhibitor (Promega, Netherlands cat#N2111) were added, and samples were run at 25°C for 10 min, 37°C for 50 min and 70°C for 15 min. Gene expressions

(17) Intersection of transient cell states with stable cell types in hippocampusbioRxivOctober 27, 2025Jack A Olmstead, Lauren E King, Brenda L Bloodgoodused during nucleus isolation contained recombinant RNAsin to preserve RNA integrity (Promega, cat. #N2111), and actinomycin D to prevent artifactual transcription stimulated by the isolation protocol (Tocris,

(18) HIV proviral transcription and infectivity are enhanced by neddylationJournal of VirologyOctober 23, 2025Cristina C. Vaca, Hannah Hudson, Isabelle Clerc, Chisu Song, Richard T. D'Aquila#210212), 10 µL 5× One-Step buffer, 10 µL 0.3% Triton-x (Fisher BP151-500), 0.25 µL 40 U/µL RNAsin (Promega N2111), 2 µL dNTPs (10 mm each), 1 µL 20 µM forward primers (tat1.4 5′ TGGCAGGAAGAAGCGGAG 3′, 1 µL 20 µM reverse

(19) Rapid remodeling of NTP levels enables immediate translational adaptation to energy stress in yeastMolecular CellOctober 2, 2025Katherine Bexley, Michaela Ristová, Sushma Sharma, Christos Spanos, Andrei Chabes, David TollerveyCartridge,Waters Sverige AB,#186002492 RNase A/T1 mix,Thermo,#EN0551 Rnasin Ribonuclease Inhibitor,Promega,#N2111 RQ1 RNase-Free DNase,Promega,#M6101 "Sucrose, Extra Pure",Thermo Scientific,#S-8560-60 Trichloroacetic acid

(20) Telomere‐Related Gene Networks in the Ovary Shift Across Environmental FactorsJournal of Experimental Zoology Part A: Ecological and Integrative PhysiologyOctober 1, 2025Sarah E. Wolf, Elizabeth M. George, Jess Dong, Kimberly A. RosvallSuperscript III reverse transcriptase (Invitrogen), treated with DNAase (Promega, Madison, WI) and RNase inhibitor (RNAsin N2111, Promega). cDNA was stored at −20°C. We then performed quantitative PCR (qPCR) to measure relative abundance

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