Description
RiboLace®
Pro is an affinity-based capture system for ribosome profiling (Ribo-seq) and translatome analysis. It uses a proprietary biotinylated puromycin derivative (3P) to selectively bind translation-engaged ribosomes, enabling magnetic enrichment of ribosome-protected fragments (RPFs) for sequencing. This targeted enrichment focuses footprint libraries on actively translated transcripts, reducing background from inactive or non-translating ribosomal complexes.
RiboLace Pro is suitable for eukaryotic cell lines and tissue samples, and the resulting ribosome-protected fragments are compatible with common RPF library preparation methods used for Ribo-seq. Revvity has validated compatibility with the NEXTFLEX™ Small RNA-Seq Kit. The enrichment step can also be integrated with other small RNA-style library preparation approaches.
RiboLace Pro enables selective enrichment of actively translating ribosomes for ribosome profiling (Ribo-seq) experiments. The technology uses a proprietary puromycin derivative (3P) coupled to magnetic beads to capture translation-engaged ribosomal complexes and the ribosome-protected fragments (RPFs) they contain following nuclease digestion. The antibody-free, tag-free enrichment workflow provides an alternative to gradient ultracentrifugation-based approaches and supports Ribo-seq sample preparation from primary or immortalized eukaryotic cell lines (fresh or frozen) as well as tissue samples. The recovered footprints are compatible with standard RPF library preparation workflows. Compatibility with the NEXTFLEX Small RNA-Seq Kit has been shown while remaining compatible with other common Ribo-seq library preparation approaches.
Key features:
Selective capture of actively translating ribosomes using 3P-functionalized magnetic beads
Antibody-free, tag-free enrichment designed to avoid ultracentrifugation-based workflows
Supports primary or immortalized eukaryotic cell lines (fresh or frozen) and tissue samples
Enriches ribosome-protected fragments (RPFs) suitable for ribosome profiling and footprint sequencing
Validated compatibility with the NEXTFLEX Small RNA-Seq Kit