Description
miND® Spike-In Controls are a ready-to-use mix of synthetic small RNAs added to total RNA before library prep. They have two core functions. First, they serve as a quality control that verifies the dynamic range and quantitative performance of the assay. Second, they enable absolute normalization of sequencing data, converting read counts to copies per microliter. This matters because small RNA sequencing from biofluids often varies in RNA content, making relative metrics like RPM (reads per million) potentially be misleading. By anchoring results in absolute units, miND Spike-Ins improve cross-sample and cross-batch comparability.
miND Spike-In Controls are comprised of seven
oligonucleotides with unique 13 nt core sequences flanked by randomized bases, supplied in defined ratios spanning typical small-RNA abundances. They are compatible with ligation-based small RNA-seq across species and matrices, including plasma, serum, urine, CSF, synovial fluid, cells, tissues, extracellular
vesicles, and non-vesicular fractions. The miND Spike-In Controls pair naturally with NEXTFLEX™ Small RNA kit as an accessory to standardize runs across studies and batches. miND Spike-In Controls calibrate and quality check small RNA-seq by adding defined small RNA standards to each sample before library prep. They verify the dynamic range of the assay and enable absolute
quantitation, converting reads into copies per microliter for more reliable cross-sample and cross-batch comparisons, especially in biofluids and other
low-input matrices.
Seven synthetic spike-ins supplied in defined ratios to span typical range of endogenous small-RNA abundances.
Unique 13-nt core with four randomized bases at both ends, designed to minimize ligation bias; 5′-phosphorylated.
Absolute quantitation via linear regression to output molecules/µL.
Simple integration: add 1 µL per RNA sample just before NEXTFLEX small RNA-seq library prep.
Broad compatibility across species and matrices including plasma, serum, urine, CSF, synovial fluid, EVs, cells, and tissues.
Simple format: 96 reaction lyophilized.
Built-in analysis support: TAmiRNA provides ready-to-use scripts and a Docker/Snakemake pipeline on GitHub to quantify miRNAs and the spike-ins, then fit a regression to report absolute unit